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Protein

Ribonuclease 3

Gene

rnc

Organism
Bacillus cereus (strain ATCC 14579 / DSM 31)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Digests double-stranded RNA. Involved in the processing of primary rRNA transcript to yield the immediate precursors to the large and small rRNAs (23S and 16S). Processes some mRNAs, and tRNAs when they are encoded in the rRNA operon. Processes pre-crRNA and tracrRNA of type II CRISPR loci if present in the organism.UniRule annotation

Catalytic activityi

Endonucleolytic cleavage to 5'-phosphomonoester.UniRule annotation

Cofactori

Mg2+UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi61 – 611MagnesiumUniRule annotation
Active sitei65 – 651UniRule annotation
Metal bindingi134 – 1341MagnesiumUniRule annotation
Active sitei137 – 1371UniRule annotation
Metal bindingi137 – 1371MagnesiumUniRule annotation

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. ribonuclease III activity Source: UniProtKB-HAMAP
  3. rRNA binding Source: UniProtKB-KW

GO - Biological processi

  1. mRNA processing Source: UniProtKB-HAMAP
  2. rRNA catabolic process Source: InterPro
  3. rRNA processing Source: UniProtKB-HAMAP
  4. tRNA processing Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

mRNA processing, rRNA processing, tRNA processing

Keywords - Ligandi

Magnesium, Metal-binding, RNA-binding, rRNA-binding

Enzyme and pathway databases

BioCyciBCER226900:GJEU-3843-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonuclease 3UniRule annotation (EC:3.1.26.3UniRule annotation)
Alternative name(s):
Ribonuclease IIIUniRule annotation
Short name:
RNase IIIUniRule annotation
Gene namesi
Name:rncUniRule annotation
Ordered Locus Names:BC_3847
OrganismiBacillus cereus (strain ATCC 14579 / DSM 31)
Taxonomic identifieri226900 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillusBacillus cereus group
ProteomesiUP000001417 Componenti: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 245245Ribonuclease 3PRO_0000180373Add
BLAST

Proteomic databases

PRIDEiQ819V8.

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi226900.BC3847.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini19 – 148130RNase IIIUniRule annotationAdd
BLAST
Domaini174 – 24370DRBMUniRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 DRBM (double-stranded RNA-binding) domain.UniRule annotation
Contains 1 RNase III domain.UniRule annotation

Phylogenomic databases

eggNOGiCOG0571.
KOiK03685.
OMAiPAHNKEF.
OrthoDBiEOG6T1WVS.

Family and domain databases

Gene3Di1.10.1520.10. 1 hit.
3.30.160.20. 1 hit.
HAMAPiMF_00104. RNase_III.
InterProiIPR014720. dsRNA-bd_dom.
IPR011907. RNase_III.
IPR000999. RNase_III_dom.
[Graphical view]
PANTHERiPTHR11207. PTHR11207. 1 hit.
PfamiPF00035. dsrm. 1 hit.
PF14622. Ribonucleas_3_3. 1 hit.
[Graphical view]
SMARTiSM00358. DSRM. 1 hit.
SM00535. RIBOc. 1 hit.
[Graphical view]
SUPFAMiSSF69065. SSF69065. 1 hit.
TIGRFAMsiTIGR02191. RNaseIII. 1 hit.
PROSITEiPS50137. DS_RBD. 1 hit.
PS00517. RNASE_3_1. 1 hit.
PS50142. RNASE_3_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q819V8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPYRKYREKK YETKYREAFK VFQEKIGITF TDEKLLIQAF THSSYVNEHR
60 70 80 90 100
KKPHEDNERL EFLGDAVLEL TVSQYLFQKY PTMSEGELTK LRAAIVCEPS
110 120 130 140 150
LVRFANELSF GSLVLLGKGE EMTGGRERPA LLADVFEAFI GALYLDQGLE
160 170 180 190 200
TVWEFLKEIV YPKINEGAFS HVMDYKSQLQ ELIQRDGSGN VEYQILQEKG
210 220 230 240
PAHNREFVSR VTLNNVALGL GSGKSKKEAE QQAAAEALKK LKEQL
Length:245
Mass (Da):28,037
Last modified:May 31, 2003 - v1
Checksum:i5667F6DB726C8FFF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016877 Genomic DNA. Translation: AAP10769.1.
RefSeqiNP_833568.1. NC_004722.1.

Genome annotation databases

EnsemblBacteriaiAAP10769; AAP10769; BC_3847.
GeneIDi1206192.
KEGGibce:BC3847.
PATRICi32603725. VBIBacCer54481_3966.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016877 Genomic DNA. Translation: AAP10769.1.
RefSeqiNP_833568.1. NC_004722.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi226900.BC3847.

Proteomic databases

PRIDEiQ819V8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAP10769; AAP10769; BC_3847.
GeneIDi1206192.
KEGGibce:BC3847.
PATRICi32603725. VBIBacCer54481_3966.

Phylogenomic databases

eggNOGiCOG0571.
KOiK03685.
OMAiPAHNKEF.
OrthoDBiEOG6T1WVS.

Enzyme and pathway databases

BioCyciBCER226900:GJEU-3843-MONOMER.

Family and domain databases

Gene3Di1.10.1520.10. 1 hit.
3.30.160.20. 1 hit.
HAMAPiMF_00104. RNase_III.
InterProiIPR014720. dsRNA-bd_dom.
IPR011907. RNase_III.
IPR000999. RNase_III_dom.
[Graphical view]
PANTHERiPTHR11207. PTHR11207. 1 hit.
PfamiPF00035. dsrm. 1 hit.
PF14622. Ribonucleas_3_3. 1 hit.
[Graphical view]
SMARTiSM00358. DSRM. 1 hit.
SM00535. RIBOc. 1 hit.
[Graphical view]
SUPFAMiSSF69065. SSF69065. 1 hit.
TIGRFAMsiTIGR02191. RNaseIII. 1 hit.
PROSITEiPS50137. DS_RBD. 1 hit.
PS00517. RNASE_3_1. 1 hit.
PS50142. RNASE_3_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 14579 / DSM 31.

Entry informationi

Entry nameiRNC_BACCR
AccessioniPrimary (citable) accession number: Q819V8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 13, 2003
Last sequence update: May 31, 2003
Last modified: March 31, 2015
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.