Q819S7 (PYRE_BACCR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Orotate phosphoribosyltransferase
Short name=OPRT
Short name=OPRTase
EC=2.4.2.10

Gene names

Name:	pyrE
Ordered Locus Names:	BC_3882

Organism

Bacillus cereus (strain ATCC 14579 / DSM 31)

Taxonomic identifier

226900 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length	210 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP) By similarity. HAMAP MF_01208
Catalytic activity	Orotidine 5'-phosphate + diphosphate = orotate + 5-phospho-alpha-D-ribose 1-diphosphate. HAMAP MF_01208
Cofactor	Magnesium By similarity. HAMAP MF_01208
Pathway	Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from orotate: step 1/2. HAMAP MF_01208
Subunit structure	Homodimer By similarity. HAMAP MF_01208
Sequence similarities	Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily.

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis
Ligand	Magnesium
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	nucleoside metabolic process Inferred from electronic annotation. Source: InterPro pyrimidine nucleotide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	orotate phosphoribosyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 210	210	Orotate phosphoribosyltransferase HAMAP MF_01208		PRO_0000110668
Regions
	Region	120 – 128	9	5-phosphoribose 1-diphosphate binding By similarity
Sites
	Binding site	94	1	5-phosphoribose 1-diphosphate; shared with dimeric partner By similarity
	Binding site	98	1	5-phosphoribose 1-diphosphate; shared with dimeric partner By similarity
	Binding site	100	1	5-phosphoribose 1-diphosphate; shared with dimeric partner By similarity
	Binding site	124	1	Orotate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q819S7 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 46DD75A86C934695	FASTA	210	22,715
	10 20 30 40 50 60 MKKEIASHLL EIGAVFLQPN DPFTWSSGMK SPIYCDNRLT LSYPKVRQAI AAGLEELIKE 70 80 90 100 110 120 HFPTVEIIAG TATAGIAHAA WVSDRMDLPM CYVRSKAKGH GKGNQIEGKA EKGQKVVVVE 130 140 150 160 170 180 DLISTGGSAI TCVEALREAG CEVLGIVSIF TYELEAGKEK LEAANVASYS LSDYSALTEV 190 200 210 AAEKGMIGQA ETKKLQEWRK NPANEAWITA

References

[1]

"Genome sequence of Bacillus cereus and comparative analysis with Bacillus anthracis." Ivanova N., Sorokin A., Anderson I., Galleron N., Candelon B., Kapatral V., Bhattacharyya A., Reznik G., Mikhailova N., Lapidus A., Chu L., Mazur M., Goltsman E., Larsen N., D'Souza M., Walunas T., Grechkin Y., Pusch G. , Haselkorn R., Fonstein M., Ehrlich S.D., Overbeek R., Kyrpides N.C. Nature 423:87-91(2003) [PubMed: 12721630] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 14579 / DSM 31.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE016877 Genomic DNA. Translation: AAP10803.1.
RefSeq	NP_833602.1. NC_004722.1.
3D structure databases
ProteinModelPortal	Q819S7.
SMR	Q819S7. Positions 1-204.
ModBase	Search...
Protein-protein interaction databases
STRING	Q819S7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000035240; EBBACP00000034446; EBBACG00000035231.
GeneID	1206227.
GenomeReviews	Gene locus BC_3882 in contig AE016877_GR.
KEGG	bce:BC3882.
PATRIC	32603801. VBIBacCer54481_4004.
Phylogenomic databases
eggNOG	COG0461.
GeneTree	EBGT00050000002725.
HOGENOM	HBG404341.
OMA	LPMTYVR.
PhylomeDB	Q819S7.
ProtClustDB	PRK00455.
Enzyme and pathway databases
BioCyc	BCER226900:BC_3882-MONOMER.
Family and domain databases
HAMAP	MF_01208. PyrE. [Tree]
InterPro	IPR004467. Or_phspho_trans_clade-1. IPR023031. Orotate_PribosylTferase. IPR000836. PRibTrfase. [Graphical view]
KO	K00762.
Pfam	PF00156. Pribosyltran. 1 hit. [Graphical view]
TIGRFAMs	TIGR00336. PyrE. 1 hit.
PROSITE	PS00103. PUR_PYR_PR_TRANSFER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PYRE_BACCR

Accession

Primary (citable) accession number: Q819S7

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 16, 2004
Last sequence update:	June 1, 2003
Last modified:	January 25, 2012
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program