Reviewed, UniProtKB/Swiss-Prot Q819Q4 (MURB1_BACCR)

Names and origin

Protein names

Recommended name:
    UDP-N-acetylenolpyruvoylglucosamine reductase 1
    EC=1.1.1.158
Alternative name(s):
    UDP-N-acetylmuramate dehydrogenase 1

Gene names

Name:	murB1
Ordered Locus Names:	BC_3909

Organism

Bacillus cereus (strain ATCC 14579 / DSM 31) [Complete proteome] [HAMAP]

Taxonomic identifier

226900 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length	301 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Cell wall formation By similarity.
Catalytic activity	UDP-N-acetylmuramate + NADP+ = UDP-N-acetyl-3-O-(1-carboxyvinyl)-D-glucosamine + NADPH. HAMAP MF_00037
Cofactor	FAD By similarity.
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00037
Subcellular location	Cytoplasm Probable.
Sequence similarities	Belongs to the murB family. Contains 1 FAD-binding PCMH-type domain.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	FAD Flavoprotein NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	FAD binding Inferred from electronic annotation. Source: InterPro UDP-N-acetylmuramate dehydrogenase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 301	301	UDP-N-acetylenolpyruvoylglucosamine reductase 1 HAMAP MF_00037		PRO_0000179174
Regions
	Domain	29 – 196	168	FAD-binding PCMH-type
Sites
	Active site	174	1	By similarity
	Active site	225	1	Proton donor By similarity
	Active site	295	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q819Q4-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: BD1C9BB7F9E331BD	FASTA	301	32,984
	10 20 30 40 50 60 MEQLVNELIE ADVGRVLVNE PLARYTTMKI GGPADILIVP KHVAGIEKTL QLVKQYKTKW 70 80 90 100 110 120 TVIGRGSNLL VSDQGIEGVV IRLGEGLDHL EVEKHKVRVG SGYPLIKLST LLSRQGLAGL 130 140 150 160 170 180 EFASGIPGSV GGAVYMNAGA HKSDISSVLS KALILFEDGA IDWLTNKELE FSYRASVLQT 190 200 210 220 230 240 KRPGIVLEAV FQLQAGKREE IVRSMQNNKD YRRETQPWNH PCAGSVFRNP IPHFAGDLVE 250 260 270 280 290 300 KAGLRGYRIG GAQISEMHGN FIVNTGGASA QDVLSLIELI KHTIKDKFDV DMHTEVEIIG R

References

[1]

"Genome sequence of Bacillus cereus and comparative analysis with Bacillus anthracis." Ivanova N., Sorokin A., Anderson I., Galleron N., Candelon B., Kapatral V., Bhattacharyya A., Reznik G., Mikhailova N., Lapidus A., Chu L., Mazur M., Goltsman E., Larsen N., D'Souza M., Walunas T., Grechkin Y., Pusch G. , Haselkorn R., Fonstein M., Ehrlich S.D., Overbeek R., Kyrpides N.C. Nature 423:87-91(2003) [PubMed: 12721630] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE016877 Genomic DNA. Translation: AAP10830.1.
RefSeq	NP_833629.1.
3D structure databases
HSSP	HSSP built from PDB template 1HSK based on UniProtKB Q93G02.
ModBase	Search...
Protein-protein interaction databases
STRING	Q819Q4.
Genome annotation databases
GeneID	1206254.
GenomeReviews	Gene locus BC_3909 in contig AE016877_GR.
KEGG	bce:BC3909.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q819Q4.
OMA	GPADILI.
Enzyme and pathway databases
BioCyc	BCER226900:BC_3909-MON.
Family and domain databases
HAMAP	MF_00037. [Tree]
InterPro	IPR016169. CO_DH_flavot_FAD-bd_sub2. IPR016166. FAD-bd_2. IPR016167. FAD-bd_2_sub1. IPR003170. MurB. IPR011601. MurB_C. IPR006094. Oxid_FAD_bind_N. [Graphical view]
Gene3D	G3DSA:3.30.465.10. CO_DH_flavoprot_FAD-bd_sub2. 1 hit. G3DSA:3.30.43.10. FAD-binding_2_sub1. 1 hit. G3DSA:3.90.78.10. MurB_C. 1 hit.
PANTHER	PTHR21071. MurB. 1 hit.
Pfam	PF01565. FAD_binding_4. 1 hit. PF02873. MurB_C. 1 hit. [Graphical view]
TIGRFAMs	TIGR00179. murB. 1 hit.
PROSITE	PS51387. FAD_PCMH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MURB1_BACCR

Accession

Primary (citable) accession number: Q819Q4

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 24, 2004
Last sequence update:	June 1, 2003
Last modified:	November 3, 2009
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)