Reviewed, UniProtKB/Swiss-Prot Q819Q2 (MURD_BACCR)

Names and origin

Protein names

Recommended name:
    UDP-N-acetylmuramoylalanine--D-glutamate ligase
    EC=6.3.2.9
Alternative name(s):
    UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase
    D-glutamic acid-adding enzyme

Gene names

Name:	murD
Ordered Locus Names:	BC_3912

Organism

Bacillus cereus (strain ATCC 14579 / DSM 31) [Complete proteome] [HAMAP]

Taxonomic identifier

226900 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length	450 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA) By similarity.
Catalytic activity	ATP + UDP-N-acetylmuramoyl-L-alanine + glutamate = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate. HAMAP MF_00639
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00639
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the murCDEF family.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP UDP-N-acetylmuramoylalanine-D-glutamate ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 450	450	UDP-N-acetylmuramoylalanine--D-glutamate ligase HAMAP MF_00639		PRO_0000108961
Regions
	Nucleotide binding	119 – 125	7	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	Q819Q2-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 5496CDAB0C92C08E	FASTA	450	48,954
	10 20 30 40 50 60 MKTVTEFQNK NILVLGIAKS GYAAATLLQK LGANVIVNDG KPLAENVLAA ELQAKGMDVV 70 80 90 100 110 120 CGGHPLELLE RNISLVVKNP GIPYSNPILV AAKEKQIPIV TEVELAYRIS EAPFVGITGS 130 140 150 160 170 180 NGKTTTTMLT FEMLKEGQKH PVIAGNIGTV ACEVAQDAKE NEVVVTELSS FQLMGVELFQ 190 200 210 220 230 240 PKIAAFLNLF EAHLDYHGTK KEYGLAKANI FKNQTENDYS VINADDADVM ALSAYSKGQK 250 260 270 280 290 300 ILFSTTKEIE DGACIKDNAL YFKGEKVIEV SDIVLPGQHN LENILAAMSI AKLLGTSNEA 310 320 330 340 350 360 ITVVLKRFTG VKHRLEYVTT INNRKFYNDS KATNMLATEK ALSAFTQPIV LLAGGLDRGN 370 380 390 400 410 420 EFDDLIPYFK NVKAIVTFGQ TAPKLVRAAE KAGLDIIESV DTLDEAVVKA YAHSKDGDVV 430 440 450 LLSPACASWD QFKTFEERGD IFIQAVHKLI

References

[1]

"Genome sequence of Bacillus cereus and comparative analysis with Bacillus anthracis." Ivanova N., Sorokin A., Anderson I., Galleron N., Candelon B., Kapatral V., Bhattacharyya A., Reznik G., Mikhailova N., Lapidus A., Chu L., Mazur M., Goltsman E., Larsen N., D'Souza M., Walunas T., Grechkin Y., Pusch G. , Haselkorn R., Fonstein M., Ehrlich S.D., Overbeek R., Kyrpides N.C. Nature 423:87-91(2003) [PubMed: 12721630] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE016877 Genomic DNA. Translation: AAP10833.1.
RefSeq	NP_833632.1.
3D structure databases
HSSP	HSSP built from PDB template 1EEH based on UniProtKB P14900.
ModBase	Search...
Protein-protein interaction databases
STRING	Q819Q2.
Genome annotation databases
GeneID	1206257.
GenomeReviews	Gene locus BC_3912 in contig AE016877_GR.
KEGG	bce:BC3912.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q819Q2.
OMA	SEDHLDR.
Enzyme and pathway databases
BioCyc	BCER226900:BC_3912-MON.
Family and domain databases
HAMAP	MF_00639. [Tree]
InterPro	IPR004101. Mur_ligase_C. IPR013221. Mur_ligase_cen. IPR016040. NAD(P)-bd_dom. IPR005762. UDP-N-AcMur-Glu_ligase. [Graphical view]
Gene3D	G3DSA:3.90.190.20. Mur_ligase_C. 1 hit. G3DSA:3.40.1190.10. Mur_ligase_cen. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF02875. Mur_ligase_C. 1 hit. PF08245. Mur_ligase_M. 1 hit. [Graphical view]
TIGRFAMs	TIGR01087. murD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MURD_BACCR

Accession

Primary (citable) accession number: Q819Q2

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 2005
Last sequence update:	June 1, 2003
Last modified:	November 3, 2009
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)