Reviewed, UniProtKB/Swiss-Prot Q819Q1 (MRAY_BACCR)

Names and origin

Protein names

Recommended name:
    Phospho-N-acetylmuramoyl-pentapeptide-transferase
    EC=2.7.8.13
Alternative name(s):
    UDP-MurNAc-pentapeptide phosphotransferase

Gene names

Name:	mraY
Ordered Locus Names:	BC_3913

Organism

Bacillus cereus (strain ATCC 14579 / DSM 31) [Complete proteome] [HAMAP]

Taxonomic identifier

226900 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length	324 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	First step of the lipid cycle reactions in the biosynthesis of the cell wall peptidoglycan By similarity.
Catalytic activity	UDP-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala) + undecaprenyl phosphate = UMP + Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol. HAMAP MF_00038
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00038
Subcellular location	Cell membrane; Multi-pass membrane protein By similarity.
Sequence similarities	Belongs to the glycosyltransferase 4 family. MraY subfamily.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cell membrane Membrane
Domain	Transmembrane
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: HAMAP
Molecular function	phospho-N-acetylmuramoyl-pentapeptide-transferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 324	324	Phospho-N-acetylmuramoyl-pentapeptide-transferase HAMAP MF_00038		PRO_0000108776
Regions
	Transmembrane	5 – 25	21	Potential
	Transmembrane	52 – 72	21	Potential
	Transmembrane	77 – 97	21	Potential
	Transmembrane	122 – 142	21	Potential
	Transmembrane	149 – 169	21	Potential
	Transmembrane	176 – 196	21	Potential
	Transmembrane	201 – 221	21	Potential
	Transmembrane	227 – 247	21	Potential
	Transmembrane	253 – 273	21	Potential
	Transmembrane	302 – 322	21	Potential

Sequences

	Sequence		Length	Mass (Da)
	Q819Q1-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 2F1B78105FC3855C	FASTA	324	34,904
	10 20 30 40 50 60 MLEQGLLVTA GVAFLISVAL SPLFIPFLRK LKFGQSIRDE GPKSHQKKSG TPTMGGIVIY 70 80 90 100 110 120 VSMMVTSLIM AIKFNYLGAE VSLLLLVTFG YGLIGFLDDY IKVVKKRNLG LTSKQKLVGQ 130 140 150 160 170 180 LVIAIAFFLI GKGQAFHTYI MIPGTDVKFE LGWAYFVLVL FMLIGGSNAV NLTDGLDGLL 190 200 210 220 230 240 SGTAAIAFGA FSIIAVAQEQ FGVAIFCMAV VGAVLGFLVF NANPAKVFMG DTGSLALGGA 250 260 270 280 290 300 IAAVAILLKQ ELLLVIIGGV FVMETLSVII QVISFKTTGK RVFKMSPLHH HYELCGWSEW 310 320 RVVVTFWSAG FLLAVLGIYI GVWM

References

[1]

"Genome sequence of Bacillus cereus and comparative analysis with Bacillus anthracis." Ivanova N., Sorokin A., Anderson I., Galleron N., Candelon B., Kapatral V., Bhattacharyya A., Reznik G., Mikhailova N., Lapidus A., Chu L., Mazur M., Goltsman E., Larsen N., D'Souza M., Walunas T., Grechkin Y., Pusch G. , Haselkorn R., Fonstein M., Ehrlich S.D., Overbeek R., Kyrpides N.C. Nature 423:87-91(2003) [PubMed: 12721630] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE016877 Genomic DNA. Translation: AAP10834.1.
RefSeq	NP_833633.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q819Q1.
Genome annotation databases
GeneID	1206258.
GenomeReviews	Gene locus BC_3913 in contig AE016877_GR.
KEGG	bce:BC3913.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q819Q1.
OMA	HHHFEQK.
Enzyme and pathway databases
BioCyc	BCER226900:BC_3913-MON.
Family and domain databases
HAMAP	MF_00038. [Tree]
InterPro	IPR000715. Glycosyl_transferase_4. IPR018481. Glycosyl_Trfase_4_cons-reg. IPR003524. PNAcMuramoyl-5peptid_Trfase. IPR018480. PNAcMuramoyl-5peptid_Trfase_CS. [Graphical view]
PANTHER	PTHR22926. Glyco_trans_4. 1 hit. PTHR22926:SF3. PNAcPpept_trans. 1 hit.
Pfam	PF00953. Glycos_transf_4. 1 hit. PF10555. MraY_sig1. 1 hit. [Graphical view]
TIGRFAMs	TIGR00445. mraY. 1 hit.
PROSITE	PS01347. MRAY_1. False negative. PS01348. MRAY_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MRAY_BACCR

Accession

Primary (citable) accession number: Q819Q1

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2005
Last sequence update:	June 1, 2003
Last modified:	November 3, 2009
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)