Reviewed, UniProtKB/Swiss-Prot Q818R9 (DXS_BACCR)

Names and origin

Protein names

Recommended name:
    1-deoxy-D-xylulose-5-phosphate synthase
    EC=2.2.1.7
Alternative name(s):
    1-deoxyxylulose-5-phosphate synthase
      Short name=DXP synthase
      Short name=DXPS

Gene names

Name:	dxs
Ordered Locus Names:	BC_4176

Organism

Bacillus cereus (strain ATCC 14579 / DSM 31) [Complete proteome] [HAMAP]

Taxonomic identifier

226900 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length	630 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the acyloin condensation reaction between C atoms 2 and 3 of pyruvate and glyceraldehyde 3-phosphate to yield 1-deoxy-D-xylulose-5-phosphate (DXP) By similarity.
Catalytic activity	Pyruvate + D-glyceraldehyde 3-phosphate = 1-deoxy-D-xylulose 5-phosphate + CO2. HAMAP MF_00315
Cofactor	Binds 1 thiamine pyrophosphate per subunit By similarity.
Pathway	Metabolic intermediate biosynthesis; 1-deoxy-D-xylulose 5-phosphate biosynthesis; 1-deoxy-D-xylulose 5-phosphate from D-glyceraldehyde 3-phosphate and pyruvate: step 1/1. HAMAP MF_00315
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the transketolase family. DXPS subfamily.

Ontologies

Keywords
Biological process	Isoprene biosynthesis Thiamine biosynthesis
Ligand	Thiamine pyrophosphate
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	terpenoid biosynthetic process Inferred from electronic annotation. Source: HAMAP thiamin biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	1-deoxy-D-xylulose-5-phosphate synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 630	630	1-deoxy-D-xylulose-5-phosphate synthase HAMAP MF_00315		PRO_0000189084

Sequences

	Sequence		Length	Mass (Da)
	Q818R9-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: A2D5568D6E170671	FASTA	630	69,411
	10 20 30 40 50 60 MDLTQIQNPS FLKDMSISEL EGLSEDIRKF LIEELSQTGG HIAPNLGVVE LTIALHKLFD 70 80 90 100 110 120 SPQDKFLWDV GHQSYVHKIL TGRAKEFGTL RQYQGLCGFP KRCESEHDVW ETGHSSTSLS 130 140 150 160 170 180 AAMGMALARD LKKTKEYVIP IIGDGALTGG MALEALNHIG HEKTDMIVIL NDNEMSIAPN 190 200 210 220 230 240 VGALHNVLGR LRTAGKYHWV KDELEYILKK IPAVGGKVAA TAEKIKDSLK YLLVSGVFFE 250 260 270 280 290 300 ELGFTYLGPV DGHDYEKLFE TLQYAKKTKG PVLVHVITKK GKGYKPAESD VIGTWHGTGP 310 320 330 340 350 360 YKIESGDFVK PKEVAPAWSA VVSETVLKLA RADERIVAIT PAMPVGSKLE KFQKEFPDRM 370 380 390 400 410 420 IDVGIAEQHA TTMAAGMATQ GMKPFLAIYS TFLQRAYDQV VHDICRQNLN VFIGIDRSGL 430 440 450 460 470 480 VGADGETHQG VFDISFLRHL PNMVLMMPKD ENEGQHLVYT AMQYEDGPIA LRYARGNGLG 490 500 510 520 530 540 VHMDEELKAI PIGTWETLKE GTQAAILTFG TTIPMAMEAA ERLEKAGVSV KVVNARFIKP 550 560 570 580 590 600 MDEAYLHDLL GKNIPILTIE EACLIGGFGT GVVEFASENG YHSALVERMG IPDRFIEHGS 610 620 630 VTKLLEEIGL TTDAVVDRIH TMIPSKQKRA

References

[1]

"Genome sequence of Bacillus cereus and comparative analysis with Bacillus anthracis." Ivanova N., Sorokin A., Anderson I., Galleron N., Candelon B., Kapatral V., Bhattacharyya A., Reznik G., Mikhailova N., Lapidus A., Chu L., Mazur M., Goltsman E., Larsen N., D'Souza M., Walunas T., Grechkin Y., Pusch G. , Haselkorn R., Fonstein M., Ehrlich S.D., Overbeek R., Kyrpides N.C. Nature 423:87-91(2003) [PubMed: 12721630] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE016877 Genomic DNA. Translation: AAP11091.1.
RefSeq	NP_833890.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q818R9.
Genome annotation databases
GeneID	1206521.
GenomeReviews	Gene locus BC_4176 in contig AE016877_GR.
KEGG	bce:BC4176.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q818R9.
OMA	QRFPDRY.
Enzyme and pathway databases
BioCyc	BCER226900:BC_4176-MON.
Family and domain databases
HAMAP	MF_00315. [Tree]
InterPro	IPR005477. Dxylulose-5-P_synthase. IPR015941. Transketolase-like_C. IPR005475. Transketolase-like_Pyr-bd. IPR005476. Transketolase_C. IPR005474. Transketolase_N. [Graphical view]
Gene3D	G3DSA:3.40.50.920. Transketo_C_like. 1 hit.
Pfam	PF02779. Transket_pyr. 1 hit. PF02780. Transketolase_C. 1 hit. [Graphical view]
TIGRFAMs	TIGR00204. dxs. 1 hit.
PROSITE	PS00801. TRANSKETOLASE_1. 1 hit. PS00802. TRANSKETOLASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DXS_BACCR

Accession

Primary (citable) accession number: Q818R9

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 16, 2004
Last sequence update:	June 1, 2003
Last modified:	November 3, 2009
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)