Q817I8 (SYN_BACCR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Asparagine--tRNA ligase
EC=6.1.1.22

Alternative name(s):
Asparaginyl-tRNA synthetase
Short name=AsnRS

Gene names

Name:	asnS
Ordered Locus Names:	BC_4559

Organism

Bacillus cereus (strain ATCC 14579 / DSM 31)

Taxonomic identifier

226900 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length	463 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-asparagine + tRNA(Asn) = AMP + diphosphate + L-asparaginyl-tRNA(Asn). HAMAP MF_00534
Subunit structure	Homodimer By similarity. HAMAP MF_00534
Subcellular location	Cytoplasm HAMAP MF_00534.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	asparaginyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW asparagine-tRNA ligase activity Inferred from electronic annotation. Source: EC nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 463	463	Asparagine--tRNA ligase HAMAP MF_00534		PRO_0000176387

Sequences

	Sequence		Length	Mass (Da)
	Q817I8 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: AA56A17402CD1F60	FASTA	463	53,040
	10 20 30 40 50 60 MENTLVKSLY RDTEKYADQT VQVSGWIRNL RDSKAFGFIE LNDGSFFKSV QIVFDTELEN 70 80 90 100 110 120 FKEIAKLPLS SSVKVEGKVI ATPGAKQPFE IKAEKIDIEG LSDSDYPLQK KRHTFEYLRT 130 140 150 160 170 180 IAHLRPRTNA FSATFRVRSI AAFAIHQFFQ ERGFVHVHTP IITGSDTEGA GEMFRVTTQD 190 200 210 220 230 240 LNNLPKGEDG KVDESKDFFG KETNLTVSGQ LNAEAYALAF RDVYTFGPTF RAENSNTTRH 250 260 270 280 290 300 AAEFWMVEPE IAFAELGDVM NLTEDMLKYA MKYVLEHAPE EMEFFNSFVD KTVLERMNNV 310 320 330 340 350 360 INSDFGRITY TEAIKVLQES GADFKYPVEW GIDLQTEHER YLSEEIFKRP VFVTDYPKDI 370 380 390 400 410 420 KAFYMRLNED GKTVAATDLL VPGIGELIGG SQREERMDVL VDRIKELGMN EEDYWWYLEL 430 440 450 460 RKYGGTKHAG FGLGFERFLM YITGMANIRD VIPFPRTPGS SEF

References

[1]

"Genome sequence of Bacillus cereus and comparative analysis with Bacillus anthracis." Ivanova N., Sorokin A., Anderson I., Galleron N., Candelon B., Kapatral V., Bhattacharyya A., Reznik G., Mikhailova N., Lapidus A., Chu L., Mazur M., Goltsman E., Larsen N., D'Souza M., Walunas T., Grechkin Y., Pusch G. , Haselkorn R., Fonstein M., Ehrlich S.D., Overbeek R., Kyrpides N.C. Nature 423:87-91(2003) [PubMed: 12721630] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 14579 / DSM 31.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE016877 Genomic DNA. Translation: AAP11466.1.
RefSeq	NP_834265.1. NC_004722.1.
3D structure databases
ProteinModelPortal	Q817I8.
ModBase	Search...
Protein-protein interaction databases
STRING	Q817I8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000032503; EBBACP00000031709; EBBACG00000032494.
GeneID	1206904.
GenomeReviews	Gene locus BC_4559 in contig AE016877_GR.
KEGG	bce:BC4559.
PATRIC	32605241. VBIBacCer54481_4719.
Phylogenomic databases
eggNOG	COG0017.
GeneTree	EBGT00050000000858.
HOGENOM	HBG745843.
OMA	AIHRFFH.
PhylomeDB	Q817I8.
ProtClustDB	PRK03932.
Enzyme and pathway databases
BioCyc	BCER226900:BC_4559-MONOMER.
Family and domain databases
HAMAP	MF_00534. Asn_tRNA_synth. [Tree]
InterPro	IPR004364. aa-tRNA-synt_II. IPR018150. aa-tRNA-synt_II-like. IPR006195. aa-tRNA-synth_II. IPR004522. Asn-tRNA-synth_IIb. IPR002312. Asp/Asn-tRNA-synth_IIb. IPR012340. NA-bd_OB-fold. IPR016027. NA-bd_OB-fold-like. IPR004365. NA-bd_OB_tRNA-helicase. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
KO	K01893.
PANTHER	PTHR22594. aa-tRNA-synt_II. 1 hit. PTHR22594:SF6. PTHR22594:SF6. 1 hit.
Pfam	PF00152. tRNA-synt_2. 1 hit. PF01336. tRNA_anti. 1 hit. [Graphical view]
PRINTS	PR01042. TRNASYNTHASP.
SUPFAM	SSF50249. Nucleic_acid_OB. 1 hit.
TIGRFAMs	TIGR00457. AsnS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYN_BACCR

Accession

Primary (citable) accession number: Q817I8

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 31, 2003
Last sequence update:	June 1, 2003
Last modified:	January 25, 2012
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program