Q817I6 (SYFA_BACCR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phenylalanine--tRNA ligase alpha chain
EC=6.1.1.20

Alternative name(s):
Phenylalanyl-tRNA synthetase alpha chain
Short name=PheRS

Gene names

Name:	pheS
Ordered Locus Names:	BC_4561

Organism

Bacillus cereus (strain ATCC 14579 / DSM 31)

Taxonomic identifier

226900 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length	344 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + L-phenylalanyl-tRNA(Phe). HAMAP MF_00281
Cofactor	Binds 2 magnesium ions per tetramer By similarity. HAMAP MF_00281
Subunit structure	Tetramer of two alpha and two beta chains By similarity.
Subcellular location	Cytoplasm HAMAP MF_00281.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 1 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	phenylalanyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW phenylalanine-tRNA ligase activity Inferred from electronic annotation. Source: EC tRNA binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 344	344	Phenylalanine--tRNA ligase alpha chain HAMAP MF_00281		PRO_0000126660
Sites
	Metal binding	256	1	Magnesium By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q817I6 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 9B2BC5DB2CD28D1E	FASTA	344	38,976
	10 20 30 40 50 60 MEARLKELKQ KALELIEEAK ELKGLNDVRV AYLGKKGPIT EVLRGMGKLS AEERPRMGAL 70 80 90 100 110 120 VNEVREAIQT RLDDKISNLE KAVIEAKLAT ETIDVTLPGR PVETGCHHPL TAVVEQIEDV 130 140 150 160 170 180 FIGMGYEVAE GTEVEKDYYN FEALNLPKDH PARDMQDTFY ITEETLLRTH TSSVQARTME 190 200 210 220 230 240 NNKEKGPIKI ICPGKVYRRD DDDATHSHQF MQIEGLVIDK NIRMSDLKGT LQVFVKKMFG 250 260 270 280 290 300 EDREIRLRPS FFPFTEPSVE MDISCMMCHG KGCGTCKGTG WIEILGAGMV HPNVLEMAGY 310 320 330 340 DSKEYQGFAF GMGAERIAML KYGVDDIRHF YTNDVRFLQQ FKRA

References

[1]

"Genome sequence of Bacillus cereus and comparative analysis with Bacillus anthracis." Ivanova N., Sorokin A., Anderson I., Galleron N., Candelon B., Kapatral V., Bhattacharyya A., Reznik G., Mikhailova N., Lapidus A., Chu L., Mazur M., Goltsman E., Larsen N., D'Souza M., Walunas T., Grechkin Y., Pusch G. , Haselkorn R., Fonstein M., Ehrlich S.D., Overbeek R., Kyrpides N.C. Nature 423:87-91(2003) [PubMed: 12721630] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 14579 / DSM 31.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE016877 Genomic DNA. Translation: AAP11468.1.
RefSeq	NP_834267.1. NC_004722.1.
3D structure databases
ProteinModelPortal	Q817I6.
SMR	Q817I6. Positions 84-342.
ModBase	Search...
Protein-protein interaction databases
STRING	Q817I6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000034256; EBBACP00000033462; EBBACG00000034247.
GeneID	1206906.
GenomeReviews	Gene locus BC_4561 in contig AE016877_GR.
KEGG	bce:BC4561.
PATRIC	32605245. VBIBacCer54481_4721.
Phylogenomic databases
eggNOG	COG0016.
GeneTree	EBGT00050000002461.
HOGENOM	HBG284353.
OMA	FRASYFP.
PhylomeDB	Q817I6.
ProtClustDB	PRK00488.
Enzyme and pathway databases
BioCyc	BCER226900:BC_4561-MONOMER.
Family and domain databases
HAMAP	MF_00281. Phe_tRNA_synth_alpha1. [Tree]
InterPro	IPR006195. aa-tRNA-synth_II. IPR004529. Phe-tRNA-synth_IIc_asu. IPR004188. Phe-tRNA_synth_II_N. IPR022911. Phe_tRNA_synth_alpha1_bac. IPR002319. Phenylalanyl-tRNA_Synthase. IPR010978. tRNA-bd_arm. [Graphical view]
KO	K01889.
Pfam	PF02912. Phe_tRNA-synt_N. 1 hit. PF01409. tRNA-synt_2d. 1 hit. [Graphical view]
SUPFAM	SSF46589. tRNA_binding_arm. 1 hit.
TIGRFAMs	TIGR00468. PheS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYFA_BACCR

Accession

Primary (citable) accession number: Q817I6

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 16, 2004
Last sequence update:	June 1, 2003
Last modified:	January 25, 2012
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program