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Protein

NAD kinase 2

Gene

nadK2

Organism
Bacillus cereus (strain ATCC 14579 / DSM 31)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in the regulation of the intracellular balance of NAD and NADP, and is a key enzyme in the biosynthesis of NADP. Catalyzes specifically the phosphorylation on 2'-hydroxyl of the adenosine moiety of NAD to yield NADP.UniRule annotation

Catalytic activityi

ATP + NAD+ = ADP + NADP+.UniRule annotation

Cofactori

a divalent metal cationUniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei52 – 521Proton acceptorUniRule annotation
Binding sitei151 – 1511NADUniRule annotation
Binding sitei153 – 1531NADUniRule annotation
Binding sitei188 – 1881NAD; via carbonyl oxygenUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi52 – 532NADUniRule annotation
Nucleotide bindingi124 – 1252NADUniRule annotation
Nucleotide bindingi164 – 1696NADUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. metal ion binding Source: UniProtKB-HAMAP
  3. NAD+ kinase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. NAD metabolic process Source: InterPro
  2. NADP biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, NAD, NADP, Nucleotide-binding

Enzyme and pathway databases

BioCyciBCER226900:GJEU-4638-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
NAD kinase 2UniRule annotation (EC:2.7.1.23UniRule annotation)
Alternative name(s):
ATP-dependent NAD kinase 2UniRule annotation
Gene namesi
Name:nadK2UniRule annotation
Ordered Locus Names:BC_4642
OrganismiBacillus cereus (strain ATCC 14579 / DSM 31)
Taxonomic identifieri226900 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillusBacillus cereus group
ProteomesiUP000001417 Componenti: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 267267NAD kinase 2PRO_0000120594Add
BLAST

Proteomic databases

PRIDEiQ817B5.

Interactioni

Protein-protein interaction databases

STRINGi226900.BC4642.

Family & Domainsi

Sequence similaritiesi

Belongs to the NAD kinase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0061.
KOiK00858.
OMAiSIRHSHE.
OrthoDBiEOG6PZXDR.

Family and domain databases

Gene3Di2.60.200.30. 1 hit.
3.40.50.10330. 1 hit.
HAMAPiMF_00361. NAD_kinase.
InterProiIPR017438. ATP-NAD_kinase_dom_1.
IPR016064. ATP-NAD_kinase_PpnK-typ.
IPR017437. ATP-NAD_kinase_PpnK-typ_all-b.
IPR002504. PolyP/ATP_NADK.
[Graphical view]
PANTHERiPTHR20275. PTHR20275. 1 hit.
PfamiPF01513. NAD_kinase. 1 hit.
[Graphical view]
SUPFAMiSSF111331. SSF111331. 1 hit.

Sequencei

Sequence statusi: Complete.

Q817B5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADRRNLFFF YGDDKATLVE KMKPIYRILE ENGFTILDHP KNANAIVSVG
60 70 80 90 100
DDATFLQAVR KTGFREDCLY AGISTKDEIS FYCDFHIDHV DTALQEITKN
110 120 130 140 150
EIEVRKYPTI QVDVDGSTSF HCLNEFSLRS SIIKTFVVDV HVDDLYFETF
160 170 180 190 200
RGDGLVVSTP TGSTAYNKSL HGAVVDPLIP CFQVSELASL NNNTYRTLGS
210 220 230 240 250
PFILNHERTL TLKLRPDGND YPVIGMDNEA LSIKQVEKAV VRLSDKQIKT
260
VKLKNNSFWE KVQRTFL
Length:267
Mass (Da):30,317
Last modified:June 1, 2003 - v1
Checksum:i47ABF8120C71E164
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016877 Genomic DNA. Translation: AAP11549.1.
RefSeqiNP_834348.1. NC_004722.1.

Genome annotation databases

EnsemblBacteriaiAAP11549; AAP11549; BC_4642.
GeneIDi1206987.
KEGGibce:BC4642.
PATRICi32605415. VBIBacCer54481_4806.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016877 Genomic DNA. Translation: AAP11549.1.
RefSeqiNP_834348.1. NC_004722.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi226900.BC4642.

Proteomic databases

PRIDEiQ817B5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAP11549; AAP11549; BC_4642.
GeneIDi1206987.
KEGGibce:BC4642.
PATRICi32605415. VBIBacCer54481_4806.

Phylogenomic databases

eggNOGiCOG0061.
KOiK00858.
OMAiSIRHSHE.
OrthoDBiEOG6PZXDR.

Enzyme and pathway databases

BioCyciBCER226900:GJEU-4638-MONOMER.

Family and domain databases

Gene3Di2.60.200.30. 1 hit.
3.40.50.10330. 1 hit.
HAMAPiMF_00361. NAD_kinase.
InterProiIPR017438. ATP-NAD_kinase_dom_1.
IPR016064. ATP-NAD_kinase_PpnK-typ.
IPR017437. ATP-NAD_kinase_PpnK-typ_all-b.
IPR002504. PolyP/ATP_NADK.
[Graphical view]
PANTHERiPTHR20275. PTHR20275. 1 hit.
PfamiPF01513. NAD_kinase. 1 hit.
[Graphical view]
SUPFAMiSSF111331. SSF111331. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 14579 / DSM 31.

Entry informationi

Entry nameiNADK2_BACCR
AccessioniPrimary (citable) accession number: Q817B5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 22, 2003
Last sequence update: June 1, 2003
Last modified: April 1, 2015
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.