Reviewed, UniProtKB/Swiss-Prot Q816X6 (MURC_BACCR)

Names and origin

Protein names

Recommended name:
    UDP-N-acetylmuramate--L-alanine ligase
    EC=6.3.2.8
Alternative name(s):
    UDP-N-acetylmuramoyl-L-alanine synthetase

Gene names

Name:	murC
Ordered Locus Names:	BC_4684

Organism

Bacillus cereus (strain ATCC 14579 / DSM 31) [Complete proteome] [HAMAP]

Taxonomic identifier

226900 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length	436 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Cell wall formation By similarity.
Catalytic activity	ATP + UDP-N-acetylmuramate + L-alanine = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanine. HAMAP MF_00046
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00046
Subcellular location	Cytoplasm Probable.
Sequence similarities	Belongs to the murCDEF family.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP UDP-N-acetylmuramate-L-alanine ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 436	436	UDP-N-acetylmuramate--L-alanine ligase HAMAP MF_00046		PRO_0000182049
Regions
	Nucleotide binding	108 – 114	7	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	Q816X6-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 70C37FCFBCFFAE74	FASTA	436	49,200
	10 20 30 40 50 60 MTVYHFVGIK GTGMSSLAQI LHDMKHTVQG SDYEKRFFTQ TALEKRSISI LPFDKNNVEE 70 80 90 100 110 120 GQVIIAGNAF PDTHEEIVAA KELNIPVHRY HHFLGDLMSQ YTSVAVTGAH GKTSTTGLLA 130 140 150 160 170 180 HVMQGAHPTS YLIGDGTGHG VENSKYFVFE ACEYRRHFLS YNPDYAIMTN IDFDHPDYFA 190 200 210 220 230 240 DINDVFSAFQ EMALQVKKGI IACGDDEELQ KIQAKVPVIF YGFGEDNDFQ ARNIQKRTDG 250 260 270 280 290 300 TIFDVFVRNT YYDTFKITGY GNHSVLNALA VIALCHYENV DVEAVKHQLT TFEGVKRRFN 310 320 330 340 350 360 EKPMGEQVII DDYAHHPTEI NATIEAARQK HPEREIVAVF QPHTFSRTEK FLDEFAESLS 370 380 390 400 410 420 KADQVYLCDI FGSARENKGE LTIEDLQKRI DGAELITDTT TDVLKKHKNG VLIFMGAGDI 430 QKFEAAYVKE VQVAEK

References

[1]

"Genome sequence of Bacillus cereus and comparative analysis with Bacillus anthracis." Ivanova N., Sorokin A., Anderson I., Galleron N., Candelon B., Kapatral V., Bhattacharyya A., Reznik G., Mikhailova N., Lapidus A., Chu L., Mazur M., Goltsman E., Larsen N., D'Souza M., Walunas T., Grechkin Y., Pusch G. , Haselkorn R., Fonstein M., Ehrlich S.D., Overbeek R., Kyrpides N.C. Nature 423:87-91(2003) [PubMed: 12721630] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE016877 Genomic DNA. Translation: AAP11591.1.
RefSeq	NP_834390.1.
3D structure databases
HSSP	HSSP built from PDB template 1J6U based on UniProtKB Q9WY73.
ModBase	Search...
Protein-protein interaction databases
STRING	Q816X6.
Genome annotation databases
GeneID	1207029.
GenomeReviews	Gene locus BC_4684 in contig AE016877_GR.
KEGG	bce:BC4684.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q816X6.
OMA	EWMVVEA.
Enzyme and pathway databases
BioCyc	BCER226900:BC_4684-MON.
Family and domain databases
HAMAP	MF_00046. [Tree]
InterPro	IPR004101. Mur_ligase_C. IPR013221. Mur_ligase_cen. IPR000713. Mur_ligase_N. IPR016040. NAD(P)-bd_dom. IPR005758. UDP-N-AcMur_Ala_ligase. [Graphical view]
Gene3D	G3DSA:3.90.190.20. Mur_ligase_C. 1 hit. G3DSA:3.40.1190.10. Mur_ligase_cen. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF01225. Mur_ligase. 1 hit. PF02875. Mur_ligase_C. 1 hit. PF08245. Mur_ligase_M. 1 hit. [Graphical view]
TIGRFAMs	TIGR01082. murC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MURC_BACCR

Accession

Primary (citable) accession number: Q816X6

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 25, 2003
Last sequence update:	June 1, 2003
Last modified:	November 3, 2009
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)