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Q814U0

- KITH_BACCR

UniProt

Q814U0 - KITH_BACCR

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Protein
Thymidine kinase
Gene
tdk, BC_5330
Organism
Bacillus cereus (strain ATCC 14579 / DSM 31)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + thymidine = ADP + thymidine 5'-phosphate.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei89 – 891Proton acceptor Reviewed prediction
Binding sitei120 – 1201Substrate; via amide nitrogen
Metal bindingi145 – 1451Zinc
Metal bindingi148 – 1481Zinc
Binding sitei179 – 1791Substrate By similarity
Metal bindingi183 – 1831Zinc
Metal bindingi186 – 1861Zinc

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi15 – 228ATP By similarity
Nucleotide bindingi88 – 914ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. thymidine kinase activity Source: UniProtKB-HAMAP
  3. zinc ion binding Source: UniProtKB-HAMAP
Complete GO annotation...

GO - Biological processi

  1. DNA replication Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

DNA synthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciBCER226900:GJEU-5322-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Thymidine kinase (EC:2.7.1.21)
Gene namesi
Name:tdk
Ordered Locus Names:BC_5330
OrganismiBacillus cereus (strain ATCC 14579 / DSM 31)
Taxonomic identifieri226900 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillusBacillus cereus group
ProteomesiUP000001417: Chromosome

Subcellular locationi

Cytoplasm Reviewed prediction UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 195195Thymidine kinaseUniRule annotation
PRO_0000174955Add
BLAST

Proteomic databases

PRIDEiQ814U0.

Interactioni

Subunit structurei

Homotetramer.1 Publication

Protein-protein interaction databases

STRINGi226900.BC5330.

Structurei

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2JA1X-ray2.80A1-195[»]
ProteinModelPortaliQ814U0.
SMRiQ814U0. Positions 1-191.

Miscellaneous databases

EvolutionaryTraceiQ814U0.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni170 – 1745Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the thymidine kinase family.

Phylogenomic databases

eggNOGiCOG1435.
KOiK00857.
OMAiQAICLSC.
OrthoDBiEOG69D3J2.

Family and domain databases

HAMAPiMF_00124. Thymidine_kinase.
InterProiIPR027417. P-loop_NTPase.
IPR001267. Thymidine_kinase.
IPR020633. Thymidine_kinase_CS.
IPR020634. Thymidine_kinase_subgr.
[Graphical view]
PANTHERiPTHR11441. PTHR11441. 1 hit.
PfamiPF00265. TK. 1 hit.
[Graphical view]
PIRSFiPIRSF035805. TK_cell. 1 hit.
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00603. TK_CELLULAR_TYPE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q814U0-1 [UniParc]FASTAAdd to Basket

« Hide

MYLINQNGWI EVICGSMFSG KSEELIRRVR RTQFAKQHAI VFKPCIDNRY    50
SEEDVVSHNG LKVKAVPVSA SKDIFEHITE ELDVIAIDEV QFFDGDIVEV 100
VQVLANRGYR VIVAGLDQDF RGLPFGQVPQ LMAIAEHVTK LQAVCSVCGS 150
PASRTQRLID GEPAAFDDPI ILVGASESYE PRCRHCHAVP ANKDK 195
Length:195
Mass (Da):21,696
Last modified:June 1, 2003 - v1
Checksum:i937F6D890890302E
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE016877 Genomic DNA. Translation: AAP12193.1.
RefSeqiNP_834992.1. NC_004722.1.

Genome annotation databases

EnsemblBacteriaiAAP12193; AAP12193; BC_5330.
GeneIDi1207670.
KEGGibce:BC5330.
PATRICi32606864. VBIBacCer54481_5503.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE016877 Genomic DNA. Translation: AAP12193.1 .
RefSeqi NP_834992.1. NC_004722.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2JA1 X-ray 2.80 A 1-195 [» ]
ProteinModelPortali Q814U0.
SMRi Q814U0. Positions 1-191.
ModBasei Search...

Protein-protein interaction databases

STRINGi 226900.BC5330.

Proteomic databases

PRIDEi Q814U0.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAP12193 ; AAP12193 ; BC_5330 .
GeneIDi 1207670.
KEGGi bce:BC5330.
PATRICi 32606864. VBIBacCer54481_5503.

Phylogenomic databases

eggNOGi COG1435.
KOi K00857.
OMAi QAICLSC.
OrthoDBi EOG69D3J2.

Enzyme and pathway databases

BioCyci BCER226900:GJEU-5322-MONOMER.

Miscellaneous databases

EvolutionaryTracei Q814U0.

Family and domain databases

HAMAPi MF_00124. Thymidine_kinase.
InterProi IPR027417. P-loop_NTPase.
IPR001267. Thymidine_kinase.
IPR020633. Thymidine_kinase_CS.
IPR020634. Thymidine_kinase_subgr.
[Graphical view ]
PANTHERi PTHR11441. PTHR11441. 1 hit.
Pfami PF00265. TK. 1 hit.
[Graphical view ]
PIRSFi PIRSF035805. TK_cell. 1 hit.
SUPFAMi SSF52540. SSF52540. 1 hit.
PROSITEi PS00603. TK_CELLULAR_TYPE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 14579 / DSM 31.
  2. "Structural studies of thymidine kinases from Bacillus anthracis and Bacillus cereus provide insights into quaternary structure and conformational changes upon substrate binding."
    Kosinska U., Carnrot C., Sandrini M.P., Clausen A.R., Wang L., Piskur J., Eriksson S., Eklund H.
    FEBS J. 274:727-737(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.80 ANGSTROMS) IN COMPLEX WITH ZINC IONS AND TTP, SUBUNIT.

Entry informationi

Entry nameiKITH_BACCR
AccessioniPrimary (citable) accession number: Q814U0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 14, 2003
Last sequence update: June 1, 2003
Last modified: May 14, 2014
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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