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Q814U0

- KITH_BACCR

UniProt

Q814U0 - KITH_BACCR

Protein

Thymidine kinase

Gene

tdk

Organism
Bacillus cereus (strain ATCC 14579 / DSM 31)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 76 (01 Oct 2014)
      Sequence version 1 (01 Jun 2003)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + thymidine = ADP + thymidine 5'-phosphate.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei89 – 891Proton acceptorUniRule annotation
    Binding sitei120 – 1201Substrate; via amide nitrogen
    Metal bindingi145 – 1451Zinc
    Metal bindingi148 – 1481Zinc
    Binding sitei179 – 1791SubstrateBy similarity
    Metal bindingi183 – 1831Zinc
    Metal bindingi186 – 1861Zinc

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi15 – 228ATPUniRule annotation
    Nucleotide bindingi88 – 914ATPUniRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-HAMAP
    2. thymidine kinase activity Source: UniProtKB-HAMAP
    3. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. DNA replication Source: UniProtKB-KW

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    DNA synthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciBCER226900:GJEU-5322-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Thymidine kinaseUniRule annotation (EC:2.7.1.21UniRule annotation)
    Gene namesi
    Name:tdkUniRule annotation
    Ordered Locus Names:BC_5330
    OrganismiBacillus cereus (strain ATCC 14579 / DSM 31)
    Taxonomic identifieri226900 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillusBacillus cereus group
    ProteomesiUP000001417: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 195195Thymidine kinasePRO_0000174955Add
    BLAST

    Proteomic databases

    PRIDEiQ814U0.

    Interactioni

    Subunit structurei

    Homotetramer.1 PublicationUniRule annotation

    Protein-protein interaction databases

    STRINGi226900.BC5330.

    Structurei

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2JA1X-ray2.80A1-195[»]
    ProteinModelPortaliQ814U0.
    SMRiQ814U0. Positions 1-191.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ814U0.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni170 – 1745Substrate binding

    Sequence similaritiesi

    Belongs to the thymidine kinase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG1435.
    KOiK00857.
    OMAiQAICLSC.
    OrthoDBiEOG69D3J2.

    Family and domain databases

    HAMAPiMF_00124. Thymidine_kinase.
    InterProiIPR027417. P-loop_NTPase.
    IPR001267. Thymidine_kinase.
    IPR020633. Thymidine_kinase_CS.
    IPR020634. Thymidine_kinase_subgr.
    [Graphical view]
    PANTHERiPTHR11441. PTHR11441. 1 hit.
    PfamiPF00265. TK. 1 hit.
    [Graphical view]
    PIRSFiPIRSF035805. TK_cell. 1 hit.
    SUPFAMiSSF52540. SSF52540. 1 hit.
    PROSITEiPS00603. TK_CELLULAR_TYPE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q814U0-1 [UniParc]FASTAAdd to Basket

    « Hide

    MYLINQNGWI EVICGSMFSG KSEELIRRVR RTQFAKQHAI VFKPCIDNRY    50
    SEEDVVSHNG LKVKAVPVSA SKDIFEHITE ELDVIAIDEV QFFDGDIVEV 100
    VQVLANRGYR VIVAGLDQDF RGLPFGQVPQ LMAIAEHVTK LQAVCSVCGS 150
    PASRTQRLID GEPAAFDDPI ILVGASESYE PRCRHCHAVP ANKDK 195
    Length:195
    Mass (Da):21,696
    Last modified:June 1, 2003 - v1
    Checksum:i937F6D890890302E
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE016877 Genomic DNA. Translation: AAP12193.1.
    RefSeqiNP_834992.1. NC_004722.1.

    Genome annotation databases

    EnsemblBacteriaiAAP12193; AAP12193; BC_5330.
    GeneIDi1207670.
    KEGGibce:BC5330.
    PATRICi32606864. VBIBacCer54481_5503.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE016877 Genomic DNA. Translation: AAP12193.1 .
    RefSeqi NP_834992.1. NC_004722.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2JA1 X-ray 2.80 A 1-195 [» ]
    ProteinModelPortali Q814U0.
    SMRi Q814U0. Positions 1-191.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 226900.BC5330.

    Proteomic databases

    PRIDEi Q814U0.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAP12193 ; AAP12193 ; BC_5330 .
    GeneIDi 1207670.
    KEGGi bce:BC5330.
    PATRICi 32606864. VBIBacCer54481_5503.

    Phylogenomic databases

    eggNOGi COG1435.
    KOi K00857.
    OMAi QAICLSC.
    OrthoDBi EOG69D3J2.

    Enzyme and pathway databases

    BioCyci BCER226900:GJEU-5322-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei Q814U0.

    Family and domain databases

    HAMAPi MF_00124. Thymidine_kinase.
    InterProi IPR027417. P-loop_NTPase.
    IPR001267. Thymidine_kinase.
    IPR020633. Thymidine_kinase_CS.
    IPR020634. Thymidine_kinase_subgr.
    [Graphical view ]
    PANTHERi PTHR11441. PTHR11441. 1 hit.
    Pfami PF00265. TK. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF035805. TK_cell. 1 hit.
    SUPFAMi SSF52540. SSF52540. 1 hit.
    PROSITEi PS00603. TK_CELLULAR_TYPE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 14579 / DSM 31.
    2. "Structural studies of thymidine kinases from Bacillus anthracis and Bacillus cereus provide insights into quaternary structure and conformational changes upon substrate binding."
      Kosinska U., Carnrot C., Sandrini M.P., Clausen A.R., Wang L., Piskur J., Eriksson S., Eklund H.
      FEBS J. 274:727-737(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.80 ANGSTROMS) IN COMPLEX WITH ZINC IONS AND TTP, SUBUNIT.

    Entry informationi

    Entry nameiKITH_BACCR
    AccessioniPrimary (citable) accession number: Q814U0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 14, 2003
    Last sequence update: June 1, 2003
    Last modified: October 1, 2014
    This is version 76 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3