Reviewed, UniProtKB/Swiss-Prot Q814T6 (MURA2_BACCR)

Names and origin

Protein names

Recommended name:
    UDP-N-acetylglucosamine 1-carboxyvinyltransferase 2
    EC=2.5.1.7
Alternative name(s):
    Enoylpyruvate transferase 2
    UDP-N-acetylglucosamine enolpyruvyl transferase 2
      Short name=EPT 2

Gene names

Name:	murA2
Ordered Locus Names:	BC_5334

Organism

Bacillus cereus (strain ATCC 14579 / DSM 31) [Complete proteome] [HAMAP]

Taxonomic identifier

226900 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length	429 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Cell wall formation. Adds enolpyruvyl to UDP-N-acetylglucosamine By similarity.
Catalytic activity	Phosphoenolpyruvate + UDP-N-acetyl-D-glucosamine = phosphate + UDP-N-acetyl-3-O-(1-carboxyvinyl)-D-glucosamine. HAMAP MF_00111
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00111
Subcellular location	Cytoplasm Probable.
Sequence similarities	Belongs to the EPSP synthase family. MurA subfamily.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	UDP-N-acetylgalactosamine biosynthetic process Inferred from electronic annotation. Source: InterPro cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	UDP-N-acetylglucosamine 1-carboxyvinyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 429	429	UDP-N-acetylglucosamine 1-carboxyvinyltransferase 2 HAMAP MF_00111		PRO_0000231156
Sites
	Active site	117	1	Proton donor By similarity
	Binding site	117	1	Phosphoenolpyruvate (covalent) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q814T6-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 0B9349FCA20E64FD	FASTA	429	45,839
	10 20 30 40 50 60 MEKLLIEGGR ALNGTIRVSG AKNSAVALIP TTILADTPVT IGGVPNISDV KMLGDLLEEI 70 80 90 100 110 120 GGKVTYGQEE EMVVDPSNMV AMPLPNGKVK KLRASYYLMG AMLGRFKKAV IGLPGGCHLG 130 140 150 160 170 180 PRPIDQHIKG FEALGAHVTN EQGAIYLRAD ELRGARIYLD VVSVGATINI MLAAVRAKGR 190 200 210 220 230 240 TVIENAAKEP EIIDVATLLT SMGARIKGAG TDVIRIDGVD SLHGCHHTII PDRIEAGTYM 250 260 270 280 290 300 ILGAASGGEV TVDNVIPQHL ESVTAKLREA GVQVETNDDQ ITVNGNRKLK VVDIKTLVYP 310 320 330 340 350 360 GFPTDLQQPF TTLLTKAHGT GVVTDTIYGA RFKHIDELRR MNAQIKVEGR SAIVTGPVLL 370 380 390 400 410 420 QGAKVKASDL RAGAALVIAG LMADGITEVT GLEHIDRGYE NIVDKLKGLG ANIWREQMTK QEIEEMKNA

References

[1]

"Genome sequence of Bacillus cereus and comparative analysis with Bacillus anthracis." Ivanova N., Sorokin A., Anderson I., Galleron N., Candelon B., Kapatral V., Bhattacharyya A., Reznik G., Mikhailova N., Lapidus A., Chu L., Mazur M., Goltsman E., Larsen N., D'Souza M., Walunas T., Grechkin Y., Pusch G. , Haselkorn R., Fonstein M., Ehrlich S.D., Overbeek R., Kyrpides N.C. Nature 423:87-91(2003) [PubMed: 12721630] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE016877 Genomic DNA. Translation: AAP12197.1.
RefSeq	NP_834996.1.
3D structure databases
HSSP	HSSP built from PDB template 1DLG based on UniProtKB P33038.
ModBase	Search...
Protein-protein interaction databases
STRING	Q814T6.
Genome annotation databases
GeneID	1207674.
GenomeReviews	Gene locus BC_5334 in contig AE016877_GR.
KEGG	bce:BC5334.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q814T6.
OMA	DNVIPTH.
Enzyme and pathway databases
BioCyc	BCER226900:BC_5334-MON.
Family and domain databases
HAMAP	MF_00111. [Tree]
InterPro	IPR001986. EPSP_synthase_core. IPR005750. UDP_GlcNAc_COvinyl_MurA. [Graphical view]
Gene3D	G3DSA:3.65.10.10. EPSP_synthase. 2 hits.
PANTHER	PTHR21090:SF4. AcGlu_Tran_MurA. 1 hit.
Pfam	PF00275. EPSP_synthase. 1 hit. [Graphical view]
ProDom	PD001867. EPSP_synth. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01072. murA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MURA2_BACCR

Accession

Primary (citable) accession number: Q814T6

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 4, 2006
Last sequence update:	June 1, 2003
Last modified:	November 3, 2009
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)