Reviewed, UniProtKB/Swiss-Prot Q814I1 (GUAC_BACCR)

Names and origin

Protein names

Recommended name:
    GMP reductase
    EC=1.7.1.7
Alternative name(s):
    Guanosine 5'-monophosphate oxidoreductase
      Short name=Guanosine monophosphate reductase

Gene names

Name:	guaC
Ordered Locus Names:	BC_5452

Organism

Bacillus cereus (strain ATCC 14579 / DSM 31) [Complete proteome] [HAMAP]

Taxonomic identifier

226900 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length	328 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the irreversible NADPH-dependent deamination of GMP to IMP. It functions in the conversion of nucleobase, nucleoside and nucleotide derivatives of G to A nucleotides, and in maintaining the intracellular balance of A and G nucleotides By similarity. HAMAP MF_01511
Catalytic activity	Inosine 5'-phosphate + NH3 + NADP+ = guanosine 5'-phosphate + NADPH. HAMAP MF_01511
Sequence similarities	Belongs to the IMPDH/GMPR family. GuaC type 2 subfamily.

Ontologies

Keywords
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	'de novo' pyrimidine base biosynthetic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW purine nucleotide metabolic process Inferred from electronic annotation. Source: HAMAP
Molecular function	GMP reductase activity Inferred from electronic annotation. Source: HAMAP dihydroorotate dehydrogenase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 328	328	GMP reductase HAMAP MF_01511		PRO_0000093748
Regions
	Nucleotide binding	205 – 228	24	NADP By similarity
Sites
	Active site	176	1	Thioimidate intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q814I1-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 10CD18A4A129E81D	FASTA	328	36,306
	10 20 30 40 50 60 MMENVFDYED IQLIPAKCIV NSRSECDTTV TLGKHKFKLP VVPANMQTII DERIATYLAE 70 80 90 100 110 120 NNYFYIMHRF QPEKRISFIR DMQSRGLIAS ISVGVKEDEY EFVQQLAAEQ LTPEYITIDI 130 140 150 160 170 180 AHGHSNAVIN MIQHIKKHLP ESFVIAGNVG TPEAVRELEN AGADATKVGI GPGKVCITKI 190 200 210 220 230 240 KTGFGTGGWQ LAALRWCAKA ASKPIIADGG IRTHGDVAKS IRFGATMVMI GSLFAGHEES 250 260 270 280 290 300 PGETIERDGK LYKEYFGSAS EFQKGEKKNV EGKKMFVEHK GSLEDTLIEM EQDLQSSISY 310 320 AGGTKLDAIR TVDYVVVKNS IFNGDKVY

References

[1]

"Genome sequence of Bacillus cereus and comparative analysis with Bacillus anthracis." Ivanova N., Sorokin A., Anderson I., Galleron N., Candelon B., Kapatral V., Bhattacharyya A., Reznik G., Mikhailova N., Lapidus A., Chu L., Mazur M., Goltsman E., Larsen N., D'Souza M., Walunas T., Grechkin Y., Pusch G. , Haselkorn R., Fonstein M., Ehrlich S.D., Overbeek R., Kyrpides N.C. Nature 423:87-91(2003) [PubMed: 12721630] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE016877 Genomic DNA. Translation: AAP12313.1.
RefSeq	NP_835112.1.
3D structure databases
SMR	Q814I1. Positions 2-322.
ModBase	Search...
Protein-protein interaction databases
STRING	Q814I1.
Genome annotation databases
GeneID	1207792.
GenomeReviews	Gene locus BC_5452 in contig AE016877_GR.
KEGG	bce:BC5452.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0516.
HOGENOM	HBG298985.
OMA	FAGHEES.
Enzyme and pathway databases
BioCyc	BCER226900:BC_5452-MONOMER.
Family and domain databases
HAMAP	MF_01511. GMP_reduct_type2. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR001295. Dihydroorotate_DH_CS. IPR005994. GMP_reduct2. IPR015875. IMP_DH/GMP_Rdtase_CS. IPR001093. IMP_DH_GMPRt. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
Pfam	PF00478. IMPDH. 1 hit. [Graphical view]
PIRSF	PIRSF036500. GMP_red_Firmic. 1 hit.
TIGRFAMs	TIGR01306. GMP_reduct_2. 1 hit.
PROSITE	PS00487. IMP_DH_GMP_RED. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GUAC_BACCR

Accession

Primary (citable) accession number: Q814I1

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2004
Last sequence update:	June 1, 2003
Last modified:	February 9, 2010
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)