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Q81487

- POLG_HCVTR

UniProt

Q81487 - POLG_HCVTR

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Protein

Genome polyprotein

Gene
N/A
Organism
Hepatitis C virus genotype 3b (isolate Tr-Kj) (HCV)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Core protein packages viral RNA to form a viral nucleocapsid, and promotes virion budding. Modulates viral translation initiation by interacting with HCV IRES and 40S ribosomal subunit. Also regulates many host cellular functions such as signaling pathways and apoptosis. Prevents the establishment of cellular antiviral state by blocking the interferon-alpha/beta (IFN-alpha/beta) and IFN-gamma signaling pathways and by inducing human STAT1 degradation. Thought to play a role in virus-mediated cell transformation leading to hepatocellular carcinomas. Interacts with, and activates STAT3 leading to cellular transformation. May repress the promoter of p53, and sequester CREB3 and SP110 isoform 3/Sp110b in the cytoplasm. Also represses cell cycle negative regulating factor CDKN1A, thereby interrupting an important check point of normal cell cycle regulation. Targets transcription factors involved in the regulation of inflammatory responses and in the immune response: suppresses NK-kappaB activation, and activates AP-1. Could mediate apoptotic pathways through association with TNF-type receptors TNFRSF1A and LTBR, although its effect on death receptor-induced apoptosis remains controversial. Enhances TRAIL mediated apoptosis, suggesting that it might play a role in immune-mediated liver cell injury. Seric core protein is able to bind C1QR1 at the T-cell surface, resulting in down-regulation of T-lymphocytes proliferation. May transactivate human MYC, Rous sarcoma virus LTR, and SV40 promoters. May suppress the human FOS and HIV-1 LTR activity. Alters lipid metabolism by interacting with hepatocellular proteins involved in lipid accumulation and storage. Core protein induces up-regulation of FAS promoter activity, and thereby probably contributes to the increased triglyceride accumulation in hepatocytes (steatosis) By similarity.
E1 and E2 glycoproteins form a heterodimer that is involved in virus attachment to the host cell, virion internalization through clathrin-dependent endocytosis and fusion with host membrane. E1/E2 heterodimer binds to human LDLR, CD81 and SCARB1/SR-BI receptors, but this binding is not sufficient for infection, some additional liver specific cofactors may be needed. The fusion function may possibly be carried by E1. E2 inhibits human EIF2AK2/PKR activation, preventing the establishment of an antiviral state. E2 is a viral ligand for CD209/DC-SIGN and CLEC4M/DC-SIGNR, which are respectively found on dendritic cells (DCs), and on liver sinusoidal endothelial cells and macrophage-like cells of lymph node sinuses. These interactions allow capture of circulating HCV particles by these cells and subsequent transmission to permissive cells. DCs act as sentinels in various tissues where they entrap pathogens and convey them to local lymphoid tissue or lymph node for establishment of immunity. Capture of circulating HCV particles by these SIGN+ cells may facilitate virus infection of proximal hepatocytes and lymphocyte subpopulations and may be essential for the establishment of persistent infection By similarity.
P7 seems to be a heptameric ion channel protein (viroporin) and is inhibited by the antiviral drug amantadine. Also inhibited by long-alkyl-chain iminosugar derivatives. Essential for infectivity By similarity.
Protease NS2-3 is a cysteine protease responsible for the autocatalytic cleavage of NS2-NS3. Seems to undergo self-inactivation following maturation By similarity.
NS3 displays three enzymatic activities: serine protease, NTPase and RNA helicase. NS3 serine protease, in association with NS4A, is responsible for the cleavages of NS3-NS4A, NS4A-NS4B, NS4B-NS5A and NS5A-NS5B. NS3/NS4A complex also prevents phosphorylation of human IRF3, thus preventing the establishment of dsRNA induced antiviral state. NS3 RNA helicase binds to RNA and unwinds dsRNA in the 3' to 5' direction, and likely RNA stable secondary structure in the template strand. Cleaves and inhibits the host antiviral protein MAVS By similarity.
NS4B induces a specific membrane alteration that serves as a scaffold for the virus replication complex. This membrane alteration gives rise to the so-called ER-derived membranous web that contains the replication complex By similarity.
NS5A is a component of the replication complex involved in RNA-binding. Its interaction with Human VAPB may target the viral replication complex to vesicles. Down-regulates viral IRES translation initiation. Mediates interferon resistance, presumably by interacting with and inhibiting human EIF2AK2/PKR. Seems to inhibit apoptosis by interacting with BIN1 and FKBP8. The hyperphosphorylated form of NS5A is an inhibitor of viral replication By similarity.
NS5B is an RNA-dependent RNA polymerase that plays an essential role in the virus replication By similarity.

Catalytic activityi

Hydrolysis of four peptide bonds in the viral precursor polyprotein, commonly with Asp or Glu in the P6 position, Cys or Thr in P1 and Ser or Ala in P1'.
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
NTP + H2O = NDP + phosphate.
ATP + H2O = ADP + phosphate.

Cofactori

Binds 1 zinc ion per NS3 protease domain By similarity.
Binds 1 zinc ion per NS5A N-terminal domain By similarity.

Enzyme regulationi

Activity of auto-protease NS2-3 is dependent on zinc ions and completely inhibited by EDTA. Serine protease NS3 is also activated by zinc ions By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei177 – 1782Cleavage; by host signal peptidase By similarity
Sitei?191 – ?1922Cleavage; by host signal peptidase Reviewed prediction
Sitei384 – 3852Cleavage; by host signal peptidase Reviewed prediction
Sitei754 – 7552Cleavage; by host signal peptidase By similarity
Sitei817 – 8182Cleavage; by host signal peptidase By similarity
Active sitei960 – 9601For protease NS2-3 activity; shared with dimeric partner By similarity
Active sitei980 – 9801For protease NS2-3 activity; shared with dimeric partner By similarity
Active sitei1001 – 10011For protease NS2-3 activity; shared with dimeric partner By similarity
Sitei1034 – 10352Cleavage; by protease NS2-3 Reviewed prediction
Active sitei1091 – 10911Charge relay system; for serine protease NS3 activity By similarity
Active sitei1115 – 11151Charge relay system; for serine protease NS3 activity By similarity
Metal bindingi1131 – 11311Zinc By similarity
Metal bindingi1133 – 11331Zinc By similarity
Active sitei1173 – 11731Charge relay system; for serine protease NS3 activity By similarity
Metal bindingi1179 – 11791Zinc By similarity
Metal bindingi1183 – 11831Zinc By similarity
Sitei1665 – 16662Cleavage; by serine protease NS3 Reviewed prediction
Sitei1719 – 17202Cleavage; by serine protease NS3 Reviewed prediction
Sitei1980 – 19812Cleavage; by serine protease NS3 Reviewed prediction
Metal bindingi2019 – 20191Zinc By similarity
Metal bindingi2037 – 20371Zinc By similarity
Metal bindingi2039 – 20391Zinc By similarity
Metal bindingi2060 – 20601Zinc By similarity
Sitei2432 – 24332Cleavage; by serine protease NS3 Reviewed prediction

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi1238 – 12458ATP Reviewed prediction

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. ATP-dependent helicase activity Source: InterPro
  3. cysteine-type endopeptidase activity Source: InterPro
  4. ion channel activity Source: UniProtKB-KW
  5. RNA binding Source: UniProtKB-KW
  6. RNA-directed RNA polymerase activity Source: UniProtKB-KW
  7. serine-type endopeptidase activity Source: InterPro
  8. serine-type exopeptidase activity Source: InterPro
  9. structural molecule activity Source: InterPro
  10. zinc ion binding Source: InterPro

GO - Biological processi

  1. apoptotic process Source: UniProtKB-KW
  2. clathrin-mediated endocytosis of virus by host cell Source: UniProtKB-KW
  3. fusion of virus membrane with host endosome membrane Source: UniProtKB-KW
  4. induction by virus of host autophagy Source: UniProtKB-KW
  5. modulation by virus of host G1/S transition checkpoint Source: UniProtKB-KW
  6. pore formation by virus in membrane of host cell Source: UniProtKB-KW
  7. protein oligomerization Source: UniProtKB-KW
  8. regulation of transcription, DNA-templated Source: UniProtKB-KW
  9. suppression by virus of host MAVS activity Source: UniProtKB-KW
  10. suppression by virus of host STAT1 activity Source: UniProtKB-KW
  11. suppression by virus of host TRAF activity Source: UniProtKB-KW
  12. suppression by virus of host type I interferon-mediated signaling pathway Source: UniProtKB-KW
  13. transcription, DNA-templated Source: UniProtKB-KW
  14. transformation of host cell by virus Source: InterPro
  15. viral RNA genome replication Source: InterPro
  16. virion attachment to host cell Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase, Ion channel, Nucleotidyltransferase, Protease, Ribonucleoprotein, RNA-directed RNA polymerase, Serine protease, Thiol protease, Transferase, Viral ion channel

Keywords - Biological processi

Activation of host autophagy by virus, Apoptosis, Clathrin-mediated endocytosis of virus by host, Fusion of virus membrane with host endosomal membrane, Fusion of virus membrane with host membrane, G1/S host cell cycle checkpoint dysregulation by virus, Host-virus interaction, Inhibition of host innate immune response by virus, Inhibition of host interferon signaling pathway by virus, Inhibition of host MAVS by virus, Inhibition of host RLR pathway by virus, Inhibition of host STAT1 by virus, Inhibition of host TRAFs by virus, Interferon antiviral system evasion, Ion transport, Modulation of host cell cycle by virus, Transcription, Transcription regulation, Transport, Viral attachment to host cell, Viral immunoevasion, Viral penetration into host cytoplasm, Viral RNA replication, Virus endocytosis by host, Virus entry into host cell

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, RNA-binding, Viral nucleoprotein, Zinc

Protein family/group databases

MEROPSiS29.001.

Names & Taxonomyi

Protein namesi
Recommended name:
Genome polyprotein
Cleaved into the following 11 chains:
Alternative name(s):
Capsid protein C
p21
Alternative name(s):
gp32
gp35
Alternative name(s):
NS1
gp68
gp70
Protease NS2-3 (EC:3.4.22.-)
Short name:
p23
Alternative name(s):
Hepacivirin
NS3P
p70
Alternative name(s):
p8
Alternative name(s):
p27
Alternative name(s):
p56
Alternative name(s):
NS5B
p68
OrganismiHepatitis C virus genotype 3b (isolate Tr-Kj) (HCV)
Taxonomic identifieri357355 [NCBI]
Taxonomic lineageiVirusesssRNA positive-strand viruses, no DNA stageFlaviviridaeHepacivirus
Virus hostiHomo sapiens (Human) [TaxID: 9606]
ProteomesiUP000007534: Genome

Subcellular locationi

Chain Core protein p21 : Host endoplasmic reticulum membrane; Single-pass membrane protein By similarity. Host mitochondrion membrane; Single-pass type I membrane protein By similarity. Host lipid droplet By similarity
Note: The C-terminal transmembrane domain of core protein p21 contains an ER signal leading the nascent polyprotein to the ER membrane. Only a minor proportion of core protein is present in the nucleus and an unknown proportion is secreted.1 Publication
Chain Core protein p19 : Virion By similarity. Host cytoplasm By similarity. Host nucleus By similarity. Secreted By similarity 1 Publication
Chain Envelope glycoprotein E1 : Virion membrane; Single-pass type I membrane protein Reviewed prediction. Host endoplasmic reticulum membrane; Single-pass type I membrane protein By similarity
Note: The C-terminal transmembrane domain acts as a signal sequence and forms a hairpin structure before cleavage by host signal peptidase. After cleavage, the membrane sequence is retained at the C-terminus of the protein, serving as ER membrane anchor. A reorientation of the second hydrophobic stretch occurs after cleavage producing a single reoriented transmembrane domain. These events explain the final topology of the protein. ER retention of E1 is leaky and, in overexpression conditions, only a small fraction reaches the plasma membrane.1 Publication
Chain Envelope glycoprotein E2 : Virion membrane; Single-pass type I membrane protein Reviewed prediction. Host endoplasmic reticulum membrane; Single-pass type I membrane protein By similarity
Note: The C-terminal transmembrane domain acts as a signal sequence and forms a hairpin structure before cleavage by host signal peptidase. After cleavage, the membrane sequence is retained at the C-terminus of the protein, serving as ER membrane anchor. A reorientation of the second hydrophobic stretch occurs after cleavage producing a single reoriented transmembrane domain. These events explain the final topology of the protein. ER retention of E2 is leaky and, in overexpression conditions, only a small fraction reaches the plasma membrane.1 Publication
Chain p7 : Host endoplasmic reticulum membrane; Multi-pass membrane protein By similarity. Host cell membrane By similarity
Note: The C-terminus of p7 membrane domain acts as a signal sequence. After cleavage by host signal peptidase, the membrane sequence is retained at the C-terminus of the protein, serving as ER membrane anchor. Only a fraction localizes to the plasma membrane.1 Publication
Chain Protease NS2-3 : Host endoplasmic reticulum membrane; Multi-pass membrane protein Reviewed prediction 1 Publication
Chain Serine protease NS3 : Host endoplasmic reticulum membrane; Peripheral membrane protein By similarity
Note: NS3 is associated to the ER membrane through its binding to NS4A.1 Publication
Chain Non-structural protein 4A : Host endoplasmic reticulum membrane; Single-pass type I membrane protein Reviewed prediction
Note: Host membrane insertion occurs after processing by the NS3 protease.1 Publication
Chain Non-structural protein 4B : Host endoplasmic reticulum membrane; Multi-pass membrane protein By similarity 1 Publication
Chain Non-structural protein 5A : Host endoplasmic reticulum membrane; Peripheral membrane protein By similarity. Host cytoplasmhost perinuclear region By similarity. Host mitochondrion By similarity
Note: Host membrane insertion occurs after processing by the NS3 protease.1 Publication
Chain RNA-directed RNA polymerase : Host endoplasmic reticulum membrane; Single-pass type I membrane protein Reviewed prediction
Note: Host membrane insertion occurs after processing by the NS3 protease.1 Publication

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini2 – 168167Cytoplasmic Reviewed predictionAdd
BLAST
Transmembranei169 – 18921Helical; Reviewed predictionAdd
BLAST
Topological domaini190 – 359170Lumenal Reviewed predictionAdd
BLAST
Transmembranei360 – 38021Helical; Reviewed predictionAdd
BLAST
Topological domaini381 – 733353Lumenal Reviewed predictionAdd
BLAST
Transmembranei734 – 75421Helical; Reviewed predictionAdd
BLAST
Topological domaini755 – 76511Lumenal Reviewed predictionAdd
BLAST
Transmembranei766 – 78621Helical; Reviewed predictionAdd
BLAST
Topological domaini787 – 7904Cytoplasmic Reviewed prediction
Transmembranei791 – 81121Helical; Reviewed predictionAdd
BLAST
Topological domaini812 – 82110Lumenal Reviewed prediction
Transmembranei822 – 84221Helical; Reviewed predictionAdd
BLAST
Topological domaini843 – 88947Cytoplasmic Reviewed predictionAdd
BLAST
Transmembranei890 – 91021Helical; Reviewed predictionAdd
BLAST
Topological domaini911 – 93626Lumenal Reviewed predictionAdd
BLAST
Transmembranei937 – 95721Helical; Reviewed predictionAdd
BLAST
Topological domaini958 – 1665708Cytoplasmic Reviewed predictionAdd
BLAST
Transmembranei1666 – 168621Helical; Reviewed predictionAdd
BLAST
Topological domaini1687 – 1813127Cytoplasmic Reviewed predictionAdd
BLAST
Transmembranei1814 – 183421Helical; Reviewed predictionAdd
BLAST
Topological domaini1835 – 18362Lumenal Reviewed prediction
Transmembranei1837 – 185721Helical; Reviewed predictionAdd
BLAST
Topological domaini1858 – 18581Cytoplasmic Reviewed prediction
Transmembranei1859 – 187921Helical; Reviewed predictionAdd
BLAST
Topological domaini1880 – 188910Lumenal Reviewed prediction
Transmembranei1890 – 191021Helical; Reviewed predictionAdd
BLAST
Topological domaini1911 – 198070Cytoplasmic Reviewed predictionAdd
BLAST
Intramembranei1981 – 201030 By similarityAdd
BLAST
Topological domaini2011 – 3002992Cytoplasmic Reviewed predictionAdd
BLAST
Transmembranei3003 – 302321Helical; By similarityAdd
BLAST

GO - Cellular componenti

  1. host cell endoplasmic reticulum membrane Source: UniProtKB-SubCell
  2. host cell lipid particle Source: UniProtKB-SubCell
  3. host cell mitochondrial membrane Source: UniProtKB-SubCell
  4. host cell nucleus Source: UniProtKB-SubCell
  5. host cell perinuclear region of cytoplasm Source: UniProtKB-SubCell
  6. host cell plasma membrane Source: UniProtKB-SubCell
  7. integral component of membrane Source: UniProtKB-KW
  8. integral to membrane of host cell Source: UniProtKB-KW
  9. ribonucleoprotein complex Source: UniProtKB-KW
  10. viral envelope Source: UniProtKB-KW
  11. viral nucleocapsid Source: UniProtKB-KW
  12. virion membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Capsid protein, Host cell membrane, Host cytoplasm, Host endoplasmic reticulum, Host lipid droplet, Host membrane, Host mitochondrion, Host nucleus, Membrane, Secreted, Viral envelope protein, Virion

Pathology & Biotechi

Keywords - Diseasei

Oncogene

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed; by host By similarity
Chaini2 – 191190Core protein p21 Reviewed predictionPRO_0000045688Add
BLAST
Chaini2 – 177176Core protein p19 By similarityPRO_0000045689Add
BLAST
Propeptidei178 – 19114ER anchor for the core protein, removed in mature form by host signal peptidase By similarityPRO_0000045690Add
BLAST
Chaini192 – 384193Envelope glycoprotein E1 Reviewed predictionPRO_0000045691Add
BLAST
Chaini385 – 754370Envelope glycoprotein E2 Reviewed predictionPRO_0000045692Add
BLAST
Chaini755 – 81763p7 By similarityPRO_0000045693Add
BLAST
Chaini818 – 1034217Protease NS2-3 Reviewed predictionPRO_0000045694Add
BLAST
Chaini1035 – 1665631Serine protease NS3 Reviewed predictionPRO_0000045695Add
BLAST
Chaini1666 – 171954Non-structural protein 4A Reviewed predictionPRO_0000045696Add
BLAST
Chaini1720 – 1980261Non-structural protein 4B Reviewed predictionPRO_0000045697Add
BLAST
Chaini1981 – 2432452Non-structural protein 5A Reviewed predictionPRO_0000045698Add
BLAST
Chaini2433 – 3023591RNA-directed RNA polymerase Reviewed predictionPRO_0000045699Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserine; by host By similarity
Modified residuei53 – 531Phosphoserine; by host By similarity
Modified residuei116 – 1161Phosphoserine; by host PKA By similarity
Glycosylationi196 – 1961N-linked (GlcNAc...); by host Reviewed prediction
Glycosylationi209 – 2091N-linked (GlcNAc...); by host Reviewed prediction
Glycosylationi235 – 2351N-linked (GlcNAc...); by host Reviewed prediction
Glycosylationi236 – 2361N-linked (GlcNAc...); by host Reviewed prediction
Glycosylationi306 – 3061N-linked (GlcNAc...); by host Reviewed prediction
Glycosylationi418 – 4181N-linked (GlcNAc...); by host Reviewed prediction
Glycosylationi424 – 4241N-linked (GlcNAc...); by host Reviewed prediction
Glycosylationi431 – 4311N-linked (GlcNAc...); by host Reviewed prediction
Glycosylationi449 – 4491N-linked (GlcNAc...); by host Reviewed prediction
Glycosylationi497 – 4971N-linked (GlcNAc...); by host Reviewed prediction
Glycosylationi534 – 5341N-linked (GlcNAc...); by host Reviewed prediction
Glycosylationi558 – 5581N-linked (GlcNAc...); by host Reviewed prediction
Glycosylationi583 – 5831N-linked (GlcNAc...); by host Reviewed prediction
Glycosylationi631 – 6311N-linked (GlcNAc...); by host Reviewed prediction
Glycosylationi653 – 6531N-linked (GlcNAc...); by host Reviewed prediction
Lipidationi1980 – 19801S-palmitoyl cysteine; by host By similarity
Disulfide bondi2122 ↔ 2170 By similarity
Modified residuei2202 – 22021Phosphoserine; by host; in p56 By similarity
Modified residuei2205 – 22051Phosphoserine; by host; in p58 By similarity
Modified residuei2209 – 22091Phosphoserine; by host; in p58 By similarity
Modified residuei2212 – 22121Phosphoserine; by host; in p58 By similarity

Post-translational modificationi

Specific enzymatic cleavages in vivo yield mature proteins. The structural proteins, core, E1, E2 and p7 are produced by proteolytic processing by host signal peptidases. The core protein is synthesized as a 21 kDa precursor which is retained in the ER membrane through the hydrophobic signal peptide. Cleavage by the signal peptidase releases the 19 kDa mature core protein. The other proteins (p7, NS2-3, NS3, NS4A, NS4B, NS5A and NS5B) are cleaved by the viral proteases By similarity.
Envelope E1 and E2 glycoproteins are highly N-glycosylated By similarity.
Core protein is phosphorylated by host PKC and PKA By similarity.
NS5A is phosphorylated in a basal form termed p56. p58 is a hyperphosphorylated form of p56. p56 and p58 coexist in the cell in roughly equivalent amounts. Hyperphosphorylation is dependent on the presence of NS4A. Human AKT1, RPS6KB1/p70S6K, MAP2K1/MEK1, MAP2K6/MKK6 and CSNK1A1/CKI-alpha kinases may be responsible for NS5A phosphorylation By similarity.
NS4B is palmitoylated. This modification may play a role in its polymerization or in protein-protein interactions By similarity.
The N-terminus of a fraction of NS4B molecules seems to be relocated post-translationally from the cytoplasm to the ER lumen, with a 5th transmembrane segment. The C-terminus of NS2 may be lumenal with a fourth transmembrane segment By similarity.
Core protein is ubiquitinated; mediated by UBE3A and leading to core protein subsequent proteasomal degradation By similarity.

Keywords - PTMi

Acetylation, Disulfide bond, Glycoprotein, Lipoprotein, Palmitate, Phosphoprotein, Ubl conjugation

Interactioni

Subunit structurei

Core protein is a homomultimer that binds the C-terminal part of E1 and interacts with numerous cellular proteins. Interaction with human STAT1 SH2 domain seems to result in decreased STAT1 phosphorylation, leading to decreased IFN-stimulated gene transcription. In addition to blocking the formation of phosphorylated STAT1, the core protein also promotes ubiquitin-mediated proteasome-dependent degradation of STAT1. Interacts with, and constitutively activates human STAT3. Associates with human LTBR and TNFRSF1A receptors and possibly induces apoptosis. Binds to human SP110 isoform 3/Sp110b, HNRPK, C1QR1, YWHAE, UBE3A/E6AP, DDX3X, APOA2 and RXRA proteins. Interacts with human CREB3 nuclear transcription protein, triggering cell transformation. May interact with human p53. Also binds human cytokeratins KRT8, KRT18, KRT19 and VIM (vimentin). E1 and E2 glycoproteins form a heterodimer that binds to human LDLR, CLDN1, CD81 and SCARB1 receptors. E2 binds and inhibits human EIF2AK2/PKR. Also binds human CD209/DC-SIGN and CLEC4M/DC-SIGNR. p7 forms a homoheptamer in vitro. NS2 forms a homodimer containing a pair of composite active sites at the dimerization interface. NS2 seems to interact with all other non-structural (NS) proteins. NS4A interacts with NS3 serine protease and stabilizes its folding. NS3-NS4A complex is essential for the activation of the latter and allows membrane anchorage of NS3. NS3 interacts with human TANK-binding kinase TBK1 and MAVS. NS4B and NS5A form homodimers and seem to interact with all other non-structural (NS) proteins. NS5A also interacts with human EIF2AK2/PKR, FKBP8, GRB2, BIN1, PIK3R1, SRCAP, VAPB and with most Src-family kinases. NS5B is a homooligomer and interacts with human VAPB, HNRNPA1 and SEPT6 By similarity.1 Publication

Structurei

3D structure databases

ProteinModelPortaliQ81487.
SMRiQ81487. Positions 2-45, 910-1034, 1037-1665, 1981-2011, 2016-2178, 2433-2998.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini907 – 1034128Peptidase C18Add
BLAST
Domaini1225 – 1377153Helicase ATP-bindingAdd
BLAST
Domaini1387 – 1546160Helicase C-terminalAdd
BLAST
Domaini2646 – 2764119RdRp catalyticAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni2 – 5958Interaction with DDX3X By similarityAdd
BLAST
Regioni2 – 2322Interaction with STAT1 By similarityAdd
BLAST
Regioni122 – 17352Interaction with APOA2 By similarityAdd
BLAST
Regioni150 – 15910Mitochondrial targeting signal By similarity
Regioni164 – 1674Important for lipid droplets localization By similarity
Regioni266 – 29732Fusion peptide Reviewed predictionAdd
BLAST
Regioni386 – 41227HVR1 By similarityAdd
BLAST
Regioni484 – 49613CD81-binding 1 Reviewed predictionAdd
BLAST
Regioni524 – 55532CD81-binding 2 Reviewed predictionAdd
BLAST
Regioni668 – 67912PKR/eIF2-alpha phosphorylation homology domain (PePHD) By similarityAdd
BLAST
Regioni1687 – 169812NS3-binding (by NS4A) Reviewed predictionAdd
BLAST
Regioni2128 – 2340213Transcriptional activation Reviewed predictionAdd
BLAST
Regioni2128 – 221689FKBP8-binding Reviewed predictionAdd
BLAST
Regioni2208 – 225851Basal phosphorylation By similarityAdd
BLAST
Regioni2218 – 228366PKR-binding Reviewed predictionAdd
BLAST
Regioni2257 – 231458NS4B-binding Reviewed predictionAdd
BLAST
Regioni2359 – 243274Basal phosphorylation By similarityAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi5 – 139Nuclear localization signal Reviewed prediction
Motifi38 – 436Nuclear localization signal Reviewed prediction
Motifi58 – 647Nuclear localization signal Reviewed prediction
Motifi66 – 716Nuclear localization signal Reviewed prediction
Motifi1324 – 13274DECH box By similarity
Motifi2330 – 23334SH3-binding Reviewed prediction
Motifi2335 – 23439Nuclear localization signal Reviewed prediction

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi831 – 8344Poly-Leu
Compositional biasi2286 – 233550Pro-richAdd
BLAST
Compositional biasi3004 – 30118Poly-Leu

Domaini

The transmembrane regions of envelope E1 and E2 glycoproteins are involved in heterodimer formation, ER localization, and assembly of these proteins. Envelope E2 glycoprotein contain a highly variable region called hypervariable region 1 (HVR1). E2 also contains two segments involved in CD81-binding. HVR1 is implicated in the SCARB1-mediated cell entry. CD81-binding regions may be involved in sensitivity and/or resistance to IFN-alpha therapy By similarity.
The N-terminus of NS5A acts as membrane anchor. The central part of NS5A seems to be intrinsically disordered and interacts with NS5B and host PKR By similarity.
The SH3-binding domain of NS5A is involved in the interaction with human Bin1, GRB2 and Src-family kinases By similarity.
The N-terminal one-third of serine protease NS3 contains the protease activity. This region contains a zinc atom that does not belong to the active site, but may play a structural rather than a catalytic role. This region is essential for the activity of protease NS2-3, maybe by contributing to the folding of the latter. The helicase activity is located in the C-terminus of NS3 By similarity.

Sequence similaritiesi

Keywords - Domaini

SH3-binding, Transmembrane, Transmembrane helix

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR011492. DEAD_Flavivir.
IPR002521. HCV_core_C.
IPR002522. HCV_core_N.
IPR002519. HCV_env.
IPR002531. HCV_NS1.
IPR002518. HCV_NS2.
IPR000745. HCV_NS4a.
IPR001490. HCV_NS4b.
IPR002868. HCV_NS5a.
IPR013193. HCV_NS5a_1B_dom.
IPR024350. HCV_NS5a_C.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR013192. NS5A_1a.
IPR027417. P-loop_NTPase.
IPR004109. Peptidase_S29.
IPR007094. RNA-dir_pol_PSvirus.
IPR002166. RNA_pol_HCV.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamiPF07652. Flavi_DEAD. 1 hit.
PF01543. HCV_capsid. 1 hit.
PF01542. HCV_core. 1 hit.
PF01539. HCV_env. 1 hit.
PF01560. HCV_NS1. 1 hit.
PF01538. HCV_NS2. 1 hit.
PF01006. HCV_NS4a. 1 hit.
PF01001. HCV_NS4b. 1 hit.
PF01506. HCV_NS5a. 1 hit.
PF08300. HCV_NS5a_1a. 1 hit.
PF08301. HCV_NS5a_1b. 1 hit.
PF12941. HCV_NS5a_C. 1 hit.
PF02907. Peptidase_S29. 1 hit.
PF00998. RdRP_3. 1 hit.
[Graphical view]
ProDomiPD001388. HCV_env. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00487. DEXDc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
SSF52540. SSF52540. 2 hits.
PROSITEiPS51693. HCV_NS2_PRO. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS50507. RDRP_SSRNA_POS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q81487-1 [UniParc]FASTAAdd to Basket

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MSTLPKPKRQ TKRNTLRRPK NVKFPAGGQI VGEVYVLPRR GPQLGVREVR     50
KTSERSQPRG RRQPTPKARP REGRSWAQPG YPWPLYGNEG CGWAGWLLPP 100
RGSRPSWGQN DPRRRSRNLG KVIDTLTCGF ADLMGYIPLI GAPVGGVARA 150
LAHGVRALED GVNYATGNLP GCSFSIFLLA LFSCLTCPAS SLEYRNASGL 200
YLLTNDCSNR SIVYEADDVI LHLPGCVPCV ETDNNNTSCW TPISPTVAVK 250
HPGVTTASIR NHVNMLVAPP TLCSALYVED AFGAVSLVGQ AFTFRPRQHK 300
TVQTCNCSIY PGHVSGHRMA WDMMMNWSPA IGLVISHLMR LPQTFFDLVV 350
GAHWGVMAGL AYFSMQGNWA KVVIVLIMFS GVDATTHTTG GSAAQATAGF 400
TSFFTRGPSQ NLQLVNSNGS WHINSTALNC NDSLNTGFIA GLFYYHKFNS 450
SGCPERMSSC KPITYFNQGW GPLTDANING PSEDRPYCWH YPPRPCNITK 500
PLNVCGPVYC FTPSPVVVGT TDIKGLPTYR FGVNESDVFL LTSLRPPQGR 550
WFGCVWMNST GFVKTCGAPP CNIYGGMKDI EANQTHLKCP TDCFRKHHDA 600
TFTRCGSGPW LTPRCLVDYP YRLWHYPCTV NFSIFKVRMF VGGHEHRFSA 650
ACNWTRGERC DLEDRDRSEQ QPLLHSTTDS LILPCSFTPM RRLSTGLIHL 700
HQNIVDVQYL YGVGSAVVGW ALKWEFVVLV FLLLADARVC VALWMMLLIS 750
QAEAAMENLV MLNALSAAGQ QGYVWYLVAF CAAWHIRGKL VPLITYGLTG 800
LWPLALLDLL LPQRAYAWTG EDDATIGAGV LLLLGFFTLS PWYKHWIGRL 850
IWWNQYAICR GEAALQVWVP PLLVRGSRDS VILLASLLYP SLIFDITKLL 900
IAVLGPLYLI QAALTSTPYF VRAHVLIRIC MLVRSAMGGK YVQMAVLTVG 950
RWFNTYLYDH LSPIQDWAAE GLKGLAVATE PVIFSPMEIK VITWGADTAA 1000
CGDILCGLPV SARLGRELLL GPADDYKKMG WRLLSPISAY AQQTRGLFGT 1050
IVTSLTGRDK NVVTGEVQVL STATQTFLGT TVGGVMWTVY HGAGSRTLAG 1100
NKRPALQMYT NVDQDLVGWP APAGTKSLDP CTCGSSDLYL VTREADVLPA 1150
RRRGDSTASL LSTRPLSCLK GSSGGPVMCP SGHVVGIFRA AVCTRGVAKA 1200
LQFIPVETLS TQVRSPSFSD NSTPPAVPES YQVGYLHAPT GSGKSTKVPA 1250
AYVAQGYSVL VLNPSVAATL GFGTYMSKAY GIDPNIRTGT RTITTGAKLT 1300
YSTYGKFLAD GGCSGGAYDV IICDECHAQD ATSILGIGTV LDQAETAGVR 1350
LTVLATATPP GSITVPHPNI EEVGLTSDGE IPFYGKALPL AMIKGGRHLV 1400
FCHSKEKCDE LASKLRGMGV NAVAFYRGLD VSVIPVSGDV VVCATDALMT 1450
GYTGDFDTVI DCNVAVEQYV DFSLDPTFSI ETRTVPQDAV SRSQRRGRTG 1500
RGRPGIYRFV TPGERPSGMF DSVVLCECYD AGCSWYDLQP AETTVRLRAY 1550
LSTPGLPVCQ DHLDFWERVF TGLTHIDAHF LSQAKQQGLN FAYLVAYQAT 1600
VCARAKASPP CWDEMWKCLI RLKPTLQGPT PLLYRLGAIQ NDICMTHPIT 1650
KYIMACMSAD LEVTTSAWVL VGGVLAALAA YCLSVGCVVI VGHIELGGKP 1700
ALVPDRQVLY QQYDEMEECS QSAPYIEQAQ AIAQQFKDKV LGLLQRASQQ 1750
EAEIRPIVQS QWQKAEAFWQ QHMWNFVSGI QYLAGLSTLP GNPAVASLMA 1800
FTASVTSPLT TNQTMFFNIL GGWVATHLAG PAASSAFVVS GLAGAAVGGI 1850
GIGRVLLDVL AGYGAGVSGA LVAFKIMGGE LPTTEDMVNL LPAILSPGAL 1900
VVGVICAAVL RRHVGPGEGA VQWMNRLIAF ASRGNHVSPT HYVPESDAAA 1950
KVTALLSSLT VTRLLRRLHQ WINEDYPSPC NGDWLHDIWD WVCIVLSDFK 2000
TWLSAKIMPK VPGIPFLSCQ KGYKGVWRGD GVMTTRCPCG EDFTGHVRNG 2050
SMRIAGSGLC ANMWHGTFPI NEYTTGPSTP VPAHNYSRAL WRVTSDSYVE 2100
VRRVGDTHYV VGATNDGLKI PCQVPAPEFF TELDGVRLHR YAPPCKPLLR 2150
DEITFSVGLH SYANGSQLSC EPEPDVAVLT SMLRDPAHIT AATAARRLAR 2200
GSPPSEASSS ASQLSAPSLK ATCQTHRPHP DAELIDANLL WRQEMGSNIT 2250
RVESETKVVI LDSFEPLRAE EDDTELSIPA ECFKKPPKYP PALPIWARPD 2300
YNPPLLPSWK DPTYEPPAVH GCALPPTRPA PVPPPRRKRT IKLDGSNVSA 2350
ALLALAERSF PSTKPEGTGT SSSGVGTEST AESGDSPETG EESDVESYSS 2400
MPPLEGEPGD PDLDADSWST VSDSEEQSVV CCSMSYSWTG AIITPCSAEE 2450
EKLPISPLSN SLLRHHNLVY STSSRSAAAR QKKVTFDRLQ VLDDHYKNVL 2500
KEVKERASGV KGRLLSFEEA CSLVPPHSGR SKYGYSAKDV RSLSSKAMNQ 2550
IRSVWEDLLE DNSTPIPTTI MAKNEVFSVN PAKGGRKPAR LIVYPDLGVR 2600
VCEKRALYDV IQKLSIATMG PAYGFQYSPK QRVEHLLKMW TSKKTPLGFS 2650
YDTRCFDSTV TEHDIRTEEG IYQCCDLEPE ARKAISALTE RLYIGGPMYN 2700
SKGLQCGYRR CRASGVLPTS FGNTITCYIK ATAASRAAGL KNPSFLVCGD 2750
DLVVISESCG VEEDRTALRA FTEAMTRYSA PPGDAPQPTY DLELISSCSS 2800
NVSVACDGAG KRYYYLTRDP ETPLARAAWE TARHTPVNSW LGNIIMFAPT 2850
IWVRMVLITH FFSILQAQEQ LERALDFEMY GATYSVTPLD LPAIIERLHG 2900
LSAFSLHGYS PTELNRVAGA LRKLGIPPLR AWRHRARAVR AKLIAQGGKA 2950
RICGLYLFNW AVRTKTKLTP LPTAGQLDLS SWFTVGVGGN DIYHSVSRAR 3000
TRHLLLCLLL LTVGVGIFLL PAR 3023
Length:3,023
Mass (Da):329,739
Last modified:January 23, 2007 - v3
Checksum:i5268D1EC410AC545
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D49374 Genomic RNA. Translation: BAA08372.1.

Cross-referencesi

Web resourcesi

euHCVdb

The European HCV database

Virus Pathogen Resource

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D49374 Genomic RNA. Translation: BAA08372.1 .

3D structure databases

ProteinModelPortali Q81487.
SMRi Q81487. Positions 2-45, 910-1034, 1037-1665, 1981-2011, 2016-2178, 2433-2998.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

MEROPSi S29.001.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Organism-specific databases

euHCVdbi D49374.

Family and domain databases

Gene3Di 3.40.50.300. 2 hits.
InterProi IPR011492. DEAD_Flavivir.
IPR002521. HCV_core_C.
IPR002522. HCV_core_N.
IPR002519. HCV_env.
IPR002531. HCV_NS1.
IPR002518. HCV_NS2.
IPR000745. HCV_NS4a.
IPR001490. HCV_NS4b.
IPR002868. HCV_NS5a.
IPR013193. HCV_NS5a_1B_dom.
IPR024350. HCV_NS5a_C.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR013192. NS5A_1a.
IPR027417. P-loop_NTPase.
IPR004109. Peptidase_S29.
IPR007094. RNA-dir_pol_PSvirus.
IPR002166. RNA_pol_HCV.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view ]
Pfami PF07652. Flavi_DEAD. 1 hit.
PF01543. HCV_capsid. 1 hit.
PF01542. HCV_core. 1 hit.
PF01539. HCV_env. 1 hit.
PF01560. HCV_NS1. 1 hit.
PF01538. HCV_NS2. 1 hit.
PF01006. HCV_NS4a. 1 hit.
PF01001. HCV_NS4b. 1 hit.
PF01506. HCV_NS5a. 1 hit.
PF08300. HCV_NS5a_1a. 1 hit.
PF08301. HCV_NS5a_1b. 1 hit.
PF12941. HCV_NS5a_C. 1 hit.
PF02907. Peptidase_S29. 1 hit.
PF00998. RdRP_3. 1 hit.
[Graphical view ]
ProDomi PD001388. HCV_env. 1 hit.
[Graphical view ] [Entries sharing at least one domain ]
SMARTi SM00487. DEXDc. 1 hit.
[Graphical view ]
SUPFAMi SSF50494. SSF50494. 1 hit.
SSF52540. SSF52540. 2 hits.
PROSITEi PS51693. HCV_NS2_PRO. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS50507. RDRP_SSRNA_POS. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Nucleotide sequence of hepatitis C virus (type 3b) isolated from a Japanese patient with chronic hepatitis C."
    Chayama K., Tsubota A., Koida I., Arase Y., Saitoh S., Ikeda K., Kumada H.
    J. Gen. Virol. 75:3623-3628(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  2. "Properties of the hepatitis C virus core protein: a structural protein that modulates cellular processes."
    McLauchlan J.
    J. Viral Hepat. 7:2-14(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVIEW.
  3. Cited for: REVIEW, SUBCELLULAR LOCATION.
  4. "An RNA-binding protein, hnRNP A1, and a scaffold protein, septin 6, facilitate hepatitis C virus replication."
    Kim C.S., Seol S.K., Song O.-K., Park J.H., Jang S.K.
    J. Virol. 81:3852-3865(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH HNRNPA1 AND SEPT6.

Entry informationi

Entry nameiPOLG_HCVTR
AccessioniPrimary (citable) accession number: Q81487
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 10, 2006
Last sequence update: January 23, 2007
Last modified: May 14, 2014
This is version 118 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Miscellaneous

Cell culture adaptation of the virus leads to mutations in NS5A, reducing its inhibitory effect on replication By similarity.
Core protein exerts viral interference on hepatitis B virus when HCV and HBV coinfect the same cell, by suppressing HBV gene expression, RNA encapsidation and budding By similarity.

Caution

The core gene probably also codes for alternative reading frame proteins (ARFPs). Many functions depicted for the core protein might belong to the ARFPs.

Keywords - Technical termi

Complete proteome, Multifunctional enzyme

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi