Reviewed, UniProtKB/Swiss-Prot Q812Y1 (MURG3_BACCR)

Names and origin

Protein names

Recommended name:
    UDP-N-acetylglucosamine--N-acetylmuramyl-(pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase 3
    EC=2.4.1.227
Alternative name(s):
    Undecaprenyl-PP-MurNAc-pentapeptide-UDPGlcNAc GlcNAc transferase 3

Gene names

Name:	murG3
Ordered Locus Names:	BC_3748

Organism

Bacillus cereus (strain ATCC 14579 / DSM 31) [Complete proteome] [HAMAP]

Taxonomic identifier

226900 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length	354 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Cell wall formation. Catalyzes the transfer of a GlcNAc subunit on undecaprenyl-pyrophosphoryl-MurNAc-pentapeptide (lipid intermediate I) to form undecaprenyl-pyrophosphoryl-MurNAc-(pentapeptide)GlcNAc (lipid intermediate II) By similarity.
Catalytic activity	UDP-N-acetylglucosamine + Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol = UDP + GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol. HAMAP MF_00033
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00033
Subcellular location	Cell membrane; Peripheral membrane protein By similarity.
Sequence similarities	Belongs to the glycosyltransferase 28 family. MurG subfamily.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cell membrane Membrane
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW lipid glycosylation Inferred from electronic annotation. Source: HAMAP peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extrinsic to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: HAMAP
Molecular function	carbohydrate binding Inferred from electronic annotation. Source: InterPro undecaprenyldiphospho-muramoylpentapeptide beta-N-acetylglucosaminyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 354	354	UDP-N-acetylglucosamine--N-acetylmuramyl-(pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase 3 HAMAP MF_00033		PRO_0000225023

Sequences

	Sequence		Length	Mass (Da)
	Q812Y1-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 2F551753D9CD4512	FASTA	354	39,757
	10 20 30 40 50 60 MSKTILFTGG GTAGHVMINI VLIPKFIEKG WRVEYIGSKN GIEKSLVQNV KYNSVSTGKL 70 80 90 100 110 120 RRYWDWDNFK DPFKIIRGCL QSYNLIKKTK PDVIFSAGGF VSVPVAIGAW LNRVPIIIRE 130 140 150 160 170 180 PDSTLGLANK IALPFATKLC TTFPQTGENV SNEKKVYVGP IVRVEIEKGN VLRGRRYCEF 190 200 210 220 230 240 QQDKPVLLIM GGSQGAKWIN DMVRECLDTI LLNFNIIHIC GKGKVDPSIG MEGYMQFEYI 250 260 270 280 290 300 GDELPHILNM ASVVVSRAGS TAISELLFLK KPMLLIPLTN SSSRGDQVLN AEYFSRQGYA 310 320 330 340 350 EVILQDRVST NTFIHAVNKL YTNKEKYIQN MNGYKKTNDE GIHQIIDIIN EVVK

References

[1]

"Genome sequence of Bacillus cereus and comparative analysis with Bacillus anthracis." Ivanova N., Sorokin A., Anderson I., Galleron N., Candelon B., Kapatral V., Bhattacharyya A., Reznik G., Mikhailova N., Lapidus A., Chu L., Mazur M., Goltsman E., Larsen N., D'Souza M., Walunas T., Grechkin Y., Pusch G. , Haselkorn R., Fonstein M., Ehrlich S.D., Overbeek R., Kyrpides N.C. Nature 423:87-91(2003) [PubMed: 12721630] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE016877 Genomic DNA. Translation: AAP10674.1.
RefSeq	NP_833473.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q812Y1.
Protein family/group databases
CAZy	GT28. Glycosyltransferase Family 28.
Genome annotation databases
GeneID	1206093.
GenomeReviews	Gene locus BC_3748 in contig AE016877_GR.
KEGG	bce:BC3748.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q812Y1.
OMA	HILNMAS.
Enzyme and pathway databases
BioCyc	BCER226900:BC_3748-MON.
Family and domain databases
HAMAP	MF_00033. [Tree]
InterPro	IPR004276. Glyco_trans_28. IPR007235. Glyco_trans_28_C. [Graphical view]
Pfam	PF04101. Glyco_tran_28_C. 1 hit. PF03033. Glyco_transf_28. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MURG3_BACCR

Accession

Primary (citable) accession number: Q812Y1

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 7, 2006
Last sequence update:	June 1, 2003
Last modified:	November 3, 2009
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)