Q812X2 (TRMD_BACCR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
tRNA (guanine-N(1)-)-methyltransferase
EC=2.1.1.228

Alternative name(s):
M1G-methyltransferase
tRNA [GM37] methyltransferase

Gene names

Name:	trmD
Ordered Locus Names:	BC_3839

Organism

Bacillus cereus (strain ATCC 14579 / DSM 31)

Taxonomic identifier

226900 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length	244 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Specifically methylates guanosine-37 in various tRNAs By similarity. HAMAP MF_00605
Catalytic activity	S-adenosyl-L-methionine + guanine(37) in tRNA = S-adenosyl-L-homocysteine + N(1)-methylguanine(37) in tRNA. HAMAP MF_00605
Subunit structure	Homodimer By similarity. HAMAP MF_00605
Subcellular location	Cytoplasm Potential HAMAP MF_00605.
Sequence similarities	Belongs to the RNA methyltransferase TrmD family.

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	RNA binding Inferred from electronic annotation. Source: InterPro tRNA (guanine-N1-)-methyltransferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 244	244	tRNA (guanine-N(1)-)-methyltransferase HAMAP MF_00605		PRO_0000060321
Regions
	Region	133 – 138	6	S-adenosyl-L-methionine binding By similarity
Sites
	Binding site	113	1	S-adenosyl-L-methionine; via amide nitrogen By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q812X2 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 1DCDCC356382949E	FASTA	244	27,966
	10 20 30 40 50 60 MKIDILTLFP DMFTGVFGSS ILKKAQEKEA VNLRVVNFRD YTTSKHNSVD DYPYGGGAGM 70 80 90 100 110 120 VLTPQPIFDA VEDLTKETER KPRVVLMCPQ GERFTQKKAE ELAGEEHLIF VCGHYEGYDE 130 140 150 160 170 180 RIREHLVTDE ISIGDYVLTG GELASMVITD SVVRLLPGVL GNHASQVEDS FSTGLLEHPH 190 200 210 220 230 240 YTRPADFRGM KVPDVLMSGN HKNIDEWRHK ESLRRTYTRR PDLLDERELS KQEKKWLEEI KREQ

References

[1]

"Genome sequence of Bacillus cereus and comparative analysis with Bacillus anthracis." Ivanova N., Sorokin A., Anderson I., Galleron N., Candelon B., Kapatral V., Bhattacharyya A., Reznik G., Mikhailova N., Lapidus A., Chu L., Mazur M., Goltsman E., Larsen N., D'Souza M., Walunas T., Grechkin Y., Pusch G. , Haselkorn R., Fonstein M., Ehrlich S.D., Overbeek R., Kyrpides N.C. Nature 423:87-91(2003) [PubMed: 12721630] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 14579 / DSM 31.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE016877 Genomic DNA. Translation: AAP10761.1.
RefSeq	NP_833560.1. NC_004722.1.
3D structure databases
ProteinModelPortal	Q812X2.
ModBase	Search...
Protein-protein interaction databases
STRING	Q812X2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000031280; EBBACP00000030486; EBBACG00000031271.
GeneID	1206184.
GenomeReviews	Gene locus BC_3839 in contig AE016877_GR.
KEGG	bce:BC3839.
PATRIC	32603709. VBIBacCer54481_3958.
Phylogenomic databases
eggNOG	COG0336.
GeneTree	EBGT00050000001149.
HOGENOM	HBG285805.
OMA	VCGRFEG.
PhylomeDB	Q812X2.
ProtClustDB	PRK00026.
Enzyme and pathway databases
BioCyc	BCER226900:BC_3839-MONOMER.
Family and domain databases
HAMAP	MF_00605. TrmD. [Tree]
InterPro	IPR016009. tRNA_m1G_MeTrfase. IPR002649. tRNA_m1G_MeTrfase_bac. IPR023148. tRNA_m1G_MeTrfase_C. [Graphical view]
Gene3D	G3DSA:1.10.1270.20. tRNA_m1G_MeTrfase_C. 1 hit.
KO	K00554.
PANTHER	PTHR10056. PTHR10056. 1 hit.
Pfam	PF01746. tRNA_m1G_MT. 1 hit. [Graphical view]
PIRSF	PIRSF000386. tRNA_mtase. 1 hit.
TIGRFAMs	TIGR00088. TrmD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TRMD_BACCR

Accession

Primary (citable) accession number: Q812X2

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 7, 2003
Last sequence update:	June 1, 2003
Last modified:	January 25, 2012
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program