Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase A

Gene

Mgat4a

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Glycosyltransferase that participates in the transfer of N-acetylglucosamine (GlcNAc) to the core mannose residues of N-linked glycans. Catalyzes the formation of the GlcNAcbeta1-4 branch on the GlcNAcbeta1-2Manalpha1-3 arm of the core structure of N-linked glycans. Essential for the production of tri- and tetra-antennary N-linked sugar chains. Involved in glucose transport by mediating SLC2A2/GLUT2 glycosylation, thereby controlling cell-surface expression of SLC2A2 in pancreatic beta cells.1 Publication

Catalytic activityi

UDP-N-acetyl-D-glucosamine + 3-(2-(N-acetyl-beta-D-glucosaminyl)-alpha-D-mannosyl)-beta-D-mannosyl-R = UDP + 3-(2,4-bis(N-acetyl-beta-D-glucosaminyl)-alpha-D-mannosyl)-beta-D-mannosyl-R.

Cofactori

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

GO - Molecular functioni

GO - Biological processi

  • N-glycan processing Source: GO_Central
  • protein N-linked glycosylation Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Ligandi

Metal-binding

Enzyme and pathway databases

ReactomeiR-MMU-975577. N-Glycan antennae elongation.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT54. Glycosyltransferase Family 54.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase A (EC:2.4.1.145)
Alternative name(s):
N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase IVa
Short name:
GlcNAc-T IVa
Short name:
GnT-IVa
Short name:
N-acetylglucosaminyltransferase IVa
UDP-N-acetylglucosamine: alpha-1,3-D-mannoside beta-1,4-N-acetylglucosaminyltransferase IVa
Cleaved into the following chain:
Gene namesi
Name:Mgat4a
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:2662992. Mgat4a.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 66CytoplasmicSequence analysis
Transmembranei7 – 2721Helical; Signal-anchor for type II membrane proteinSequence analysisAdd
BLAST
Topological domaini28 – 535508LumenalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane, Secreted

Pathology & Biotechi

Disruption phenotypei

Mice display impaired beta cell glucose transport and insulin secretion causing type 2 diabete. This is due to defects in SLC2A2/Glut-2 glycosylation, provoking SLC2A2/Glut-2 endocytosis with redistribution into endosomes and lysosomes.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 535535Alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase APRO_0000288585Add
BLAST
Chaini93 – 535443Alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase A soluble formBy similarityPRO_0000288586Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi77 – 771N-linked (GlcNAc...)Sequence analysis
Glycosylationi458 – 4581N-linked (GlcNAc...)Sequence analysis
Modified residuei474 – 4741PhosphoserineBy similarity

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiQ812G0.
PaxDbiQ812G0.
PRIDEiQ812G0.

PTM databases

PhosphoSiteiQ812G0.

Expressioni

Gene expression databases

BgeeiENSMUSG00000026110.
ExpressionAtlasiQ812G0. baseline and differential.
GenevisibleiQ812G0. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000038894.

Structurei

3D structure databases

ProteinModelPortaliQ812G0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili28 – 6336Sequence analysisAdd
BLAST

Sequence similaritiesi

Belongs to the glycosyltransferase 54 family.Curated

Keywords - Domaini

Coiled coil, Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IEDC. Eukaryota.
ENOG410XPV0. LUCA.
GeneTreeiENSGT00550000074578.
HOGENOMiHOG000015085.
HOVERGENiHBG053194.
InParanoidiQ812G0.
KOiK00738.
OMAiLQVPNIY.
OrthoDBiEOG091G06KB.
PhylomeDBiQ812G0.
TreeFamiTF324570.

Family and domain databases

InterProiIPR006759. Glyco_transf_54.
[Graphical view]
PANTHERiPTHR12062. PTHR12062. 1 hit.
PfamiPF04666. Glyco_transf_54. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q812G0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRLRNGTVAT ALVFVTSFLT LSWYTTWQNG KEKLIAYQRE FLALKERLRV
60 70 80 90 100
AEHRISQRSS ELNTIVQQFR RAGAETNGSK TALSTISDNT IKLLKELTSK
110 120 130 140 150
KSLRVPSIYY HLPHLLQNER SLQPAVQIGS GRTGVSIVMG IPTVKREVKS
160 170 180 190 200
YLVETLHSLI DNLYPEEKLD CVIVVFIGET DLDYVHSVVA NLEKEFSREI
210 220 230 240 250
SSGLLEIISP PESYYPDLTN LKETFGDSKE RVRWRTKQNL DYCFLMMYAQ
260 270 280 290 300
EKGIYYIQLE DDIIVKQNYF NTIKNFALQL SSEEWMILEF SQLGFIGKMF
310 320 330 340 350
QAPDLALVVE FILMFYKEKP IDWLLDHILW VKVCNPEKDA KHCDRQKANL
360 370 380 390 400
RIRFRPSLFQ HVGLHSSLSG KIQKLTDKDY MKPLLLKVHV NPPAEVSTSL
410 420 430 440 450
KVYQGHTLEK TYMGEDFFWA ITPTAGDYIL FKFDKPVNVE SYLFHSGNQE
460 470 480 490 500
HPGDILLNTT VDVLPLKSDS LEISKETKDK RLEDGYFRIG KFEYGVAEGI
510 520 530
VDPGLNPISA FRLSVIQNSA VWAILNEIHI KKVTS
Length:535
Mass (Da):61,480
Last modified:June 1, 2003 - v1
Checksum:iD6E0CA37825795C2
GO
Isoform 2 (identifier: Q812G0-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     79-88: SKTALSTISD → N

Show »
Length:526
Mass (Da):60,590
Checksum:i352E5C85E588BA1A
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti487 – 4871F → S in BAE37301 (PubMed:16141072).Curated
Sequence conflicti504 – 5041G → A in BAE37301 (PubMed:16141072).Curated
Sequence conflicti522 – 5221W → R in BAE37301 (PubMed:16141072).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei79 – 8810SKTALSTISD → N in isoform 2. 1 PublicationVSP_025715

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB053217 mRNA. Translation: BAC55018.1.
AB053225 Genomic DNA. Translation: BAC55085.1.
AK163327 mRNA. Translation: BAE37301.1.
CCDSiCCDS14893.1. [Q812G0-1]
CCDS69882.1. [Q812G0-2]
RefSeqiNP_001277730.1. NM_001290801.1. [Q812G0-2]
NP_776295.1. NM_173870.3. [Q812G0-1]
XP_006496085.1. XM_006496022.2. [Q812G0-1]
XP_011236828.1. XM_011238526.1. [Q812G0-2]
UniGeneiMm.25861.
Mm.447744.

Genome annotation databases

EnsembliENSMUST00000042161; ENSMUSP00000038894; ENSMUSG00000026110. [Q812G0-1]
ENSMUST00000151952; ENSMUSP00000114175; ENSMUSG00000026110. [Q812G0-1]
ENSMUST00000154819; ENSMUSP00000121181; ENSMUSG00000026110. [Q812G0-2]
GeneIDi269181.
KEGGimmu:269181.
UCSCiuc007arr.2. mouse. [Q812G0-1]
uc007aru.2. mouse. [Q812G0-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB053217 mRNA. Translation: BAC55018.1.
AB053225 Genomic DNA. Translation: BAC55085.1.
AK163327 mRNA. Translation: BAE37301.1.
CCDSiCCDS14893.1. [Q812G0-1]
CCDS69882.1. [Q812G0-2]
RefSeqiNP_001277730.1. NM_001290801.1. [Q812G0-2]
NP_776295.1. NM_173870.3. [Q812G0-1]
XP_006496085.1. XM_006496022.2. [Q812G0-1]
XP_011236828.1. XM_011238526.1. [Q812G0-2]
UniGeneiMm.25861.
Mm.447744.

3D structure databases

ProteinModelPortaliQ812G0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000038894.

Protein family/group databases

CAZyiGT54. Glycosyltransferase Family 54.

PTM databases

PhosphoSiteiQ812G0.

Proteomic databases

MaxQBiQ812G0.
PaxDbiQ812G0.
PRIDEiQ812G0.

Protocols and materials databases

DNASUi269181.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000042161; ENSMUSP00000038894; ENSMUSG00000026110. [Q812G0-1]
ENSMUST00000151952; ENSMUSP00000114175; ENSMUSG00000026110. [Q812G0-1]
ENSMUST00000154819; ENSMUSP00000121181; ENSMUSG00000026110. [Q812G0-2]
GeneIDi269181.
KEGGimmu:269181.
UCSCiuc007arr.2. mouse. [Q812G0-1]
uc007aru.2. mouse. [Q812G0-2]

Organism-specific databases

CTDi11320.
MGIiMGI:2662992. Mgat4a.

Phylogenomic databases

eggNOGiENOG410IEDC. Eukaryota.
ENOG410XPV0. LUCA.
GeneTreeiENSGT00550000074578.
HOGENOMiHOG000015085.
HOVERGENiHBG053194.
InParanoidiQ812G0.
KOiK00738.
OMAiLQVPNIY.
OrthoDBiEOG091G06KB.
PhylomeDBiQ812G0.
TreeFamiTF324570.

Enzyme and pathway databases

UniPathwayiUPA00378.
ReactomeiR-MMU-975577. N-Glycan antennae elongation.

Miscellaneous databases

ChiTaRSiMgat4a. mouse.
PROiQ812G0.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000026110.
ExpressionAtlasiQ812G0. baseline and differential.
GenevisibleiQ812G0. MM.

Family and domain databases

InterProiIPR006759. Glyco_transf_54.
[Graphical view]
PANTHERiPTHR12062. PTHR12062. 1 hit.
PfamiPF04666. Glyco_transf_54. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMGT4A_MOUSE
AccessioniPrimary (citable) accession number: Q812G0
Secondary accession number(s): Q3TQT1, Q80UI0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: June 1, 2003
Last modified: September 7, 2016
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.