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Protein

Alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase B

Gene

Mgat4b

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Glycosyltransferase that participates in the transfer of N-acetylglucosamine (GlcNAc) to the core mannose residues of N-linked glycans. Catalyzes the formation of the GlcNAcbeta1-4 branch on the GlcNAcbeta1-2Manalpha1-3 arm of the core structure of N-linked glycans. Essential for the production of tri- and tetra-antennary N-linked sugar chains. Has lower affinities for donors or acceptors than MGAT4A, suggesting that, under physiological conditions, it is not the main contributor in N-glycan biosynthesis (By similarity).By similarity

Catalytic activityi

UDP-N-acetyl-D-glucosamine + 3-(2-(N-acetyl-beta-D-glucosaminyl)-alpha-D-mannosyl)-beta-D-mannosyl-R = UDP + 3-(2,4-bis(N-acetyl-beta-D-glucosaminyl)-alpha-D-mannosyl)-beta-D-mannosyl-R.

Cofactori

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

GO - Molecular functioni

GO - Biological processi

  • protein N-linked glycosylation Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Ligandi

Metal-binding

Enzyme and pathway databases

ReactomeiR-MMU-975577. N-Glycan antennae elongation.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT54. Glycosyltransferase Family 54.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase B (EC:2.4.1.145)
Alternative name(s):
N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase IVb
Short name:
GlcNAc-T IVb
Short name:
GnT-IVb
Short name:
N-acetylglucosaminyltransferase IVb
UDP-N-acetylglucosamine: alpha-1,3-D-mannoside beta-1,4-N-acetylglucosaminyltransferase IVb
Gene namesi
Name:Mgat4b
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:2143974. Mgat4b.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 77CytoplasmicSequence analysis
Transmembranei8 – 2821Helical; Signal-anchor for type II membrane proteinSequence analysisAdd
BLAST
Topological domaini29 – 548520LumenalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 548548Alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase BPRO_0000288594Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi87 – 871N-linked (GlcNAc...)Sequence analysis
Glycosylationi103 – 1031N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiQ812F8.
PaxDbiQ812F8.
PRIDEiQ812F8.

PTM databases

iPTMnetiQ812F8.
PhosphoSiteiQ812F8.

Expressioni

Gene expression databases

BgeeiQ812F8.
ExpressionAtlasiQ812F8. baseline and differential.
GenevisibleiQ812F8. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000043346.

Structurei

3D structure databases

ProteinModelPortaliQ812F8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili36 – 8348Sequence analysisAdd
BLAST

Sequence similaritiesi

Belongs to the glycosyltransferase 54 family.Curated

Keywords - Domaini

Coiled coil, Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IEDC. Eukaryota.
ENOG410XPV0. LUCA.
GeneTreeiENSGT00550000074578.
HOGENOMiHOG000015085.
HOVERGENiHBG053194.
InParanoidiQ812F8.
KOiK00738.
OMAiLKLWNIT.
OrthoDBiEOG70ZZN3.
PhylomeDBiQ812F8.
TreeFamiTF324570.

Family and domain databases

InterProiIPR006759. Glyco_transf_54.
[Graphical view]
PANTHERiPTHR12062. PTHR12062. 1 hit.
PfamiPF04666. Glyco_transf_54. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q812F8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRLRNGTFLT LLLFCLCAFL SLSWYAALSG QKGDVVDIYQ REFLALRDRL
60 70 80 90 100
HAAEQESLKR SKELNLVLEE IKRAVSERQA LRDGEGNRTW GRLTEDPRLK
110 120 130 140 150
PWNVSHRHVL HLPTVFHHLP HLLAKESSLQ PAVRVGQGRT GVSVVMGIPS
160 170 180 190 200
VRREVHSYLT DTLHSLISEL SPQEKEDSVI VVLIAETDPQ YTSAVTENIK
210 220 230 240 250
ALFPTEIHSG LLEVISPSPH FYPDFSRLRE SFGDPKERVR WRTKQNLDYC
260 270 280 290 300
FLMMYAQSKG IYYVQLEDDI VAKPNYLSTM KNFALQQPSE DWMILEFSQL
310 320 330 340 350
GFIGKMFKSL DLSLIVEFIL MFYRDKPIDW LLDHILWVKV CNPEKDAKHC
360 370 380 390 400
DRQKANLRIR FKPSLFQHVG THSSLAGKIQ KLKDKDFGKH ALRKEHVNPP
410 420 430 440 450
AEVSTSLKTY QHFTLEKAYL REDFFWAFTP AAGDFIRFRF FQPLRLERFF
460 470 480 490 500
FRSGNIEHPE DKLFNTSVEV LPFDNPQSEK EALQEGRSAT LRYPRSPDGY
510 520 530 540
LQIGSFYKGV AEGEVDPAFG PLEALRLSIQ TDSPVWVILS EIFLKKAD
Length:548
Mass (Da):63,302
Last modified:June 1, 2003 - v1
Checksum:iA66F88F4A05FBF27
GO

Sequence cautioni

The sequence AAH26638.1 differs from that shown. Reason: Erroneous initiation. Curated
The sequence AAH31613.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti395 – 3962EH → SY in BAC55019 (PubMed:10372966).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB053218 mRNA. Translation: BAC55019.1.
AB053219 Genomic DNA. Translation: BAC55020.1.
AL627187 Genomic DNA. Translation: CAI25119.1.
AK155336 mRNA. Translation: BAE33202.1.
BC026638 mRNA. Translation: AAH26638.1. Different initiation.
BC031613 mRNA. Translation: AAH31613.1. Different initiation.
CCDSiCCDS24630.1.
RefSeqiNP_666038.3. NM_145926.3.
UniGeneiMm.86759.

Genome annotation databases

EnsembliENSMUST00000041725; ENSMUSP00000043346; ENSMUSG00000036620.
GeneIDi103534.
KEGGimmu:103534.
UCSCiuc007iry.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB053218 mRNA. Translation: BAC55019.1.
AB053219 Genomic DNA. Translation: BAC55020.1.
AL627187 Genomic DNA. Translation: CAI25119.1.
AK155336 mRNA. Translation: BAE33202.1.
BC026638 mRNA. Translation: AAH26638.1. Different initiation.
BC031613 mRNA. Translation: AAH31613.1. Different initiation.
CCDSiCCDS24630.1.
RefSeqiNP_666038.3. NM_145926.3.
UniGeneiMm.86759.

3D structure databases

ProteinModelPortaliQ812F8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000043346.

Protein family/group databases

CAZyiGT54. Glycosyltransferase Family 54.

PTM databases

iPTMnetiQ812F8.
PhosphoSiteiQ812F8.

Proteomic databases

MaxQBiQ812F8.
PaxDbiQ812F8.
PRIDEiQ812F8.

Protocols and materials databases

DNASUi103534.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000041725; ENSMUSP00000043346; ENSMUSG00000036620.
GeneIDi103534.
KEGGimmu:103534.
UCSCiuc007iry.2. mouse.

Organism-specific databases

CTDi11282.
MGIiMGI:2143974. Mgat4b.

Phylogenomic databases

eggNOGiENOG410IEDC. Eukaryota.
ENOG410XPV0. LUCA.
GeneTreeiENSGT00550000074578.
HOGENOMiHOG000015085.
HOVERGENiHBG053194.
InParanoidiQ812F8.
KOiK00738.
OMAiLKLWNIT.
OrthoDBiEOG70ZZN3.
PhylomeDBiQ812F8.
TreeFamiTF324570.

Enzyme and pathway databases

UniPathwayiUPA00378.
ReactomeiR-MMU-975577. N-Glycan antennae elongation.

Miscellaneous databases

ChiTaRSiMgat4b. mouse.
NextBioi355989.
PROiQ812F8.
SOURCEiSearch...

Gene expression databases

BgeeiQ812F8.
ExpressionAtlasiQ812F8. baseline and differential.
GenevisibleiQ812F8. MM.

Family and domain databases

InterProiIPR006759. Glyco_transf_54.
[Graphical view]
PANTHERiPTHR12062. PTHR12062. 1 hit.
PfamiPF04666. Glyco_transf_54. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A novel second isoenzyme of the human UDP-N-acetylglucosamine:alpha1,3-D-mannoside beta1,4-N-acetylglucosaminyltransferase family: cDNA cloning, expression, and chromosomal assignment."
    Yoshida A., Minowa M.T., Takamatsu S., Hara T., Ikenaga H., Takeuchi M.
    Glycoconj. J. 15:1115-1123(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
    Strain: 129/SvJ.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-495.
    Strain: NOD.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 208-548.
    Strain: FVB/N.
    Tissue: Colon.

Entry informationi

Entry nameiMGT4B_MOUSE
AccessioniPrimary (citable) accession number: Q812F8
Secondary accession number(s): Q3U2D9, Q812F9, Q8R0L4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: June 1, 2003
Last modified: May 11, 2016
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.