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Protein

Neuronal membrane glycoprotein M6-a

Gene

Gpm6a

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in neuronal differentiation, including differentiation and migration of neuronal stem cells (By similarity). Plays a role in neuronal plasticity and is involved in neurite and filopodia outgrowth, filopodia motility and probably synapse formation. Gpm6a-induced filopodia formation involves mitogen-activated protein kinase (MAPK) and Src signaling pathways. May be involved in neuronal NGF-dependent Ca2+ influx. May be involved in regulation of endocytosis and intracellular trafficking of G-protein-coupled receptors (GPCRs); enhances internalization and recycling of mu-type opioid receptor.By similarity6 Publications

GO - Molecular functioni

  • calcium channel activity Source: RGD

GO - Biological processi

  • neural retina development Source: UniProtKB
  • neuron migration Source: UniProtKB
  • neuron projection morphogenesis Source: UniProtKB
  • positive regulation of filopodium assembly Source: UniProtKB
  • stem cell differentiation Source: UniProtKB
  • synapse assembly Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Neurogenesis

Names & Taxonomyi

Protein namesi
Recommended name:
Neuronal membrane glycoprotein M6-a
Short name:
M6a
Gene namesi
Name:Gpm6a
Synonyms:m6a
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 16

Organism-specific databases

RGDi631368. Gpm6a.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 22CytoplasmicSequence analysisAdd BLAST22
Transmembranei23 – 43HelicalSequence analysisAdd BLAST21
Topological domaini44 – 84ExtracellularSequence analysisAdd BLAST41
Transmembranei85 – 105HelicalSequence analysisAdd BLAST21
Topological domaini106 – 127CytoplasmicSequence analysisAdd BLAST22
Transmembranei128 – 148HelicalSequence analysisAdd BLAST21
Topological domaini149 – 213Extracellular1 PublicationAdd BLAST65
Transmembranei214 – 234HelicalSequence analysisAdd BLAST21
Topological domaini235 – 278CytoplasmicSequence analysisAdd BLAST44

GO - Cellular componenti

  • axonal growth cone Source: UniProtKB
  • dendritic spine Source: UniProtKB-SubCell
  • extracellular exosome Source: Ensembl
  • filopodium Source: UniProtKB
  • integral component of membrane Source: UniProtKB-KW
  • neuronal cell body Source: UniProtKB
  • neuron projection Source: UniProtKB
  • plasma membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cell projection, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi10T → A: Reduces motility of Gpm6a-induced filopodia; when associated with A-256, A-267 and A-268. 1 Publication1
Mutagenesisi162C → A: Abolishess cell surface expression. 1 Publication1
Mutagenesisi174C → A: Impairs Gpm6a-induced filopodium formation. 1 Publication1
Mutagenesisi174C → A: Impairs synaptic density in primary hippocampal neurons; when associated with A-192. 1 Publication1
Mutagenesisi192C → A: Impairs Gpm6a-induced filopodium formation. 1 Publication1
Mutagenesisi192C → A: Impairs synaptic density in primary hippocampal neurons; when associated with A-174. 1 Publication1
Mutagenesisi202C → A: Abolishess cell surface expression. 1 Publication1
Mutagenesisi256S → A: Reduces motility of Gpm6a-induced filopodia; when associated with A-10, A-267 and A-268. 1 Publication1
Mutagenesisi267S → A: Reduces motility of Gpm6a-induced filopodia; when associated with A-10, A-256 and A-268. 1 Publication1
Mutagenesisi268T → A: Reduces motility of Gpm6a-induced filopodia; when associated with A-10, A-256 and A-267. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004180161 – 278Neuronal membrane glycoprotein M6-aAdd BLAST278

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Glycosylationi164N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi174 ↔ 1921 Publication
Glycosylationi208N-linked (GlcNAc...)Sequence analysis1
Modified residuei256PhosphoserineCombined sources1
Modified residuei278PhosphothreonineCombined sources1

Keywords - PTMi

Acetylation, Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ812E9.
PRIDEiQ812E9.

PTM databases

iPTMnetiQ812E9.
PhosphoSitePlusiQ812E9.
SwissPalmiQ812E9.

Expressioni

Tissue specificityi

Expressed in hippocampus (at protein level). Isoform 1 is the predominant isoform expressed in brain, specifically in hippocampus. Isoform 2 is expressed at low levels in brain and kidney.2 Publications

Inductioni

Isoform 1 is down-regulated by chronic stress in dentate gyrus granule neurons and CA3 pyramidal neurons whereas isoform 2 is up-regulated in the medial prefrontal cortex.1 Publication

Gene expression databases

BgeeiENSRNOG00000010731.
GenevisibleiQ812E9. RN.

Interactioni

Subunit structurei

Interacts with OPRM1.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
Oprm1P335357EBI-6113756,EBI-4392569

Protein-protein interaction databases

BioGridi258452. 1 interactor.
IntActiQ812E9. 1 interactor.
STRINGi10116.ENSRNOP00000014312.

Family & Domainsi

Sequence similaritiesi

Belongs to the myelin proteolipid protein family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG4800. Eukaryota.
ENOG4110EPW. LUCA.
GeneTreeiENSGT00390000006915.
HOGENOMiHOG000231338.
HOVERGENiHBG000096.
InParanoidiQ812E9.
OMAiEEKKVCT.
OrthoDBiEOG091G0IM5.
PhylomeDBiQ812E9.
TreeFamiTF315162.

Family and domain databases

InterProiIPR001614. Myelin_PLP.
IPR018237. Myelin_PLP_CS.
[Graphical view]
PANTHERiPTHR11683. PTHR11683. 1 hit.
PfamiPF01275. Myelin_PLP. 1 hit.
[Graphical view]
PRINTSiPR00214. MYELINPLP.
SMARTiSM00002. PLP. 1 hit.
[Graphical view]
PROSITEiPS00575. MYELIN_PLP_1. 1 hit.
PS01004. MYELIN_PLP_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q812E9-1) [UniParc]FASTAAdd to basket
Also known as: Ib

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEENMEEGQT QKGCFECCIK CLGGIPYASL IATILLYAGV ALFCGCGHEA
60 70 80 90 100
LSGTVNILQT YFEMARTAGD TLDVFTMIDI FKYVIYGIAA AFFVYGILLM
110 120 130 140 150
VEGFFTTGAI KDLYGDFKIT TCGRCVSAWF IMLTYLFMLA WLGVTAFTSL
160 170 180 190 200
PVYMYFNVWT ICRNTTLVEG ANLCLDLRQF GIVTIGEEKK ICTVSENFLR
210 220 230 240 250
MCESTELNMT FHLFIVALAG AGAAVIAMVH YLMVLSANWA YVKDACRMQK
260 270
YEDIKSKEEQ ELHDIHSTRS KERLNAYT
Length:278
Mass (Da):31,196
Last modified:June 1, 2003 - v1
Checksum:iD2EDAF98C0E8715D
GO
Isoform 2 (identifier: Q812E9-2) [UniParc]FASTAAdd to basket
Also known as: Ia

The sequence of this isoform differs from the canonical sequence as follows:
     1-12: MEENMEEGQTQK → M

Show »
Length:267
Mass (Da):29,891
Checksum:i9B3D31D6AFADF061
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0439571 – 12MEENM…GQTQK → M in isoform 2. 1 PublicationAdd BLAST12

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB089242 mRNA. Translation: BAC56699.1.
CH473995 Genomic DNA. Translation: EDL78956.1.
BC088862 mRNA. Translation: AAH88862.1.
DV216104 mRNA. No translation available.
RefSeqiNP_835206.1. NM_178105.2. [Q812E9-1]
XP_017455597.1. XM_017600108.1. [Q812E9-2]
UniGeneiRn.34370.

Genome annotation databases

EnsembliENSRNOT00000014312; ENSRNOP00000014312; ENSRNOG00000010731. [Q812E9-1]
ENSRNOT00000092971; ENSRNOP00000075962; ENSRNOG00000010731. [Q812E9-2]
GeneIDi306439.
KEGGirno:306439.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB089242 mRNA. Translation: BAC56699.1.
CH473995 Genomic DNA. Translation: EDL78956.1.
BC088862 mRNA. Translation: AAH88862.1.
DV216104 mRNA. No translation available.
RefSeqiNP_835206.1. NM_178105.2. [Q812E9-1]
XP_017455597.1. XM_017600108.1. [Q812E9-2]
UniGeneiRn.34370.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi258452. 1 interactor.
IntActiQ812E9. 1 interactor.
STRINGi10116.ENSRNOP00000014312.

PTM databases

iPTMnetiQ812E9.
PhosphoSitePlusiQ812E9.
SwissPalmiQ812E9.

Proteomic databases

PaxDbiQ812E9.
PRIDEiQ812E9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000014312; ENSRNOP00000014312; ENSRNOG00000010731. [Q812E9-1]
ENSRNOT00000092971; ENSRNOP00000075962; ENSRNOG00000010731. [Q812E9-2]
GeneIDi306439.
KEGGirno:306439.

Organism-specific databases

CTDi2823.
RGDi631368. Gpm6a.

Phylogenomic databases

eggNOGiKOG4800. Eukaryota.
ENOG4110EPW. LUCA.
GeneTreeiENSGT00390000006915.
HOGENOMiHOG000231338.
HOVERGENiHBG000096.
InParanoidiQ812E9.
OMAiEEKKVCT.
OrthoDBiEOG091G0IM5.
PhylomeDBiQ812E9.
TreeFamiTF315162.

Miscellaneous databases

PROiQ812E9.

Gene expression databases

BgeeiENSRNOG00000010731.
GenevisibleiQ812E9. RN.

Family and domain databases

InterProiIPR001614. Myelin_PLP.
IPR018237. Myelin_PLP_CS.
[Graphical view]
PANTHERiPTHR11683. PTHR11683. 1 hit.
PfamiPF01275. Myelin_PLP. 1 hit.
[Graphical view]
PRINTSiPR00214. MYELINPLP.
SMARTiSM00002. PLP. 1 hit.
[Graphical view]
PROSITEiPS00575. MYELIN_PLP_1. 1 hit.
PS01004. MYELIN_PLP_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGPM6A_RAT
AccessioniPrimary (citable) accession number: Q812E9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 13, 2012
Last sequence update: June 1, 2003
Last modified: November 30, 2016
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.