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Protein

Glutamine-dependent NAD(+) synthetase

Gene

Nadsyn1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

ATP + deamido-NAD+ + L-glutamine + H2O = AMP + diphosphate + NAD+ + L-glutamate.

Pathwayi: NAD(+) biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes NAD(+) from deamido-NAD(+) (L-Gln route).
Proteins known to be involved in this subpathway in this organism are:
  1. Glutamine-dependent NAD(+) synthetase (Nadsyn1)
This subpathway is part of the pathway NAD(+) biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes NAD(+) from deamido-NAD(+) (L-Gln route), the pathway NAD(+) biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei357 – 3571PROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi355 – 3628ATPBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Ligandi

ATP-binding, NAD, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00253; UER00334.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamine-dependent NAD(+) synthetase (EC:6.3.5.1)
Alternative name(s):
NAD(+) synthase [glutamine-hydrolyzing]
NAD(+) synthetase
Gene namesi
Name:Nadsyn1
Synonyms:Qns1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi727801. Nadsyn1.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 725725Glutamine-dependent NAD(+) synthetasePRO_0000237580Add
BLAST

Proteomic databases

PaxDbiQ812E8.
PRIDEiQ812E8.

Interactioni

Subunit structurei

Homohexamer.By similarity

Protein-protein interaction databases

BioGridi261873. 1 interaction.
STRINGi10116.ENSRNOP00000028175.

Structurei

3D structure databases

ProteinModelPortaliQ812E8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini5 – 299295CN hydrolasePROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni325 – 706382LigaseBy similarityAdd
BLAST

Sequence similaritiesi

In the C-terminal section; belongs to the NAD synthetase family.Curated
Contains 1 CN hydrolase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG2303. Eukaryota.
COG0171. LUCA.
COG0388. LUCA.
HOVERGENiHBG082007.
InParanoidiQ812E8.
KOiK01950.
PhylomeDBiQ812E8.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
3.60.110.10. 1 hit.
InterProiIPR003010. C-N_Hydrolase.
IPR014445. Gln-dep_NAD_synthase.
IPR022310. NAD/GMP_synthase.
IPR003694. NAD_synthase.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00795. CN_hydrolase. 1 hit.
PF02540. NAD_synthase. 1 hit.
[Graphical view]
PIRSFiPIRSF006630. NADS_GAT. 1 hit.
SUPFAMiSSF56317. SSF56317. 1 hit.
TIGRFAMsiTIGR00552. nadE. 1 hit.
PROSITEiPS50263. CN_HYDROLASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q812E8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGRKVTVATC ALNQWALDFE GNFQRILKSI QIAKGKGARY RLGPELEICG
60 70 80 90 100
YGCWDHYHES DTLLHSLQVL AALLDAPATQ DIICDVGMPI MHRNVRYNCL
110 120 130 140 150
VIFLNRKILL IRPKMALANE GNYRELRWFT PWARSRQTEE YVLPRMLQDL
160 170 180 190 200
TKQETVPFGD VVLATQDTCI GSEICEELWT PCSPHVNMGL DGVEIITNAS
210 220 230 240 250
GSHHVLRKAH TRVDLVTMAT SKNGGIYLLA NQKGCDGHLL YYDGCAMIAM
260 270 280 290 300
NGSIFAQGTQ FSLDDVEVLT ATLDLEDVRS YRAKISSRNL EATRVNPYPR
310 320 330 340 350
VTVDFALSVS EDLLEPVSEP VEWTYHRPEE EISLGPACWL WDFLRRNNQA
360 370 380 390 400
GFFLPLSGGV DSAASACVVY SMCCLVCEAV KSGNQQVLTD VQNLVDESSY
410 420 430 440 450
TPQDPRELCG RLLTTCYMAS ENSSQETHNR ATELAQQIGS YHISLNIDPA
460 470 480 490 500
VKAILGIFSL VTGKFPRFSA HGGSSRENLA LQNVQARIRM VLAYLFAQLS
510 520 530 540 550
LWSRGARGSL LVLGSANVDE SLLGYLTKYD CSSADINPIG GISKTDLRAF
560 570 580 590 600
VQLCAERFQL PVLQAILSAP ATAELEPLAD GQVSQMDEED MGMTYTELSI
610 620 630 640 650
FGRLRKVAKA GPYSMFCKLL NMWKDSCTPR QVAEKVKRFF SKYSINRHKM
660 670 680 690 700
TTLTPAYHAE NYSPDDNRFD LRPFLYNTRW PWQFLCIDNQ VVQLERKTSQ
710 720
TLEEQIQEHF KEPSPIWKQL LPKDP
Length:725
Mass (Da):81,511
Last modified:June 1, 2003 - v1
Checksum:i4CA8EAFCE183871A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB090805 mRNA. Translation: BAC57897.1.
RefSeqiNP_852145.1. NM_181480.1.
UniGeneiRn.22886.

Genome annotation databases

GeneIDi353255.
KEGGirno:353255.
UCSCiRGD:727801. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB090805 mRNA. Translation: BAC57897.1.
RefSeqiNP_852145.1. NM_181480.1.
UniGeneiRn.22886.

3D structure databases

ProteinModelPortaliQ812E8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi261873. 1 interaction.
STRINGi10116.ENSRNOP00000028175.

Proteomic databases

PaxDbiQ812E8.
PRIDEiQ812E8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi353255.
KEGGirno:353255.
UCSCiRGD:727801. rat.

Organism-specific databases

CTDi55191.
RGDi727801. Nadsyn1.

Phylogenomic databases

eggNOGiKOG2303. Eukaryota.
COG0171. LUCA.
COG0388. LUCA.
HOVERGENiHBG082007.
InParanoidiQ812E8.
KOiK01950.
PhylomeDBiQ812E8.

Enzyme and pathway databases

UniPathwayiUPA00253; UER00334.

Miscellaneous databases

NextBioi672643.
PROiQ812E8.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
3.60.110.10. 1 hit.
InterProiIPR003010. C-N_Hydrolase.
IPR014445. Gln-dep_NAD_synthase.
IPR022310. NAD/GMP_synthase.
IPR003694. NAD_synthase.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00795. CN_hydrolase. 1 hit.
PF02540. NAD_synthase. 1 hit.
[Graphical view]
PIRSFiPIRSF006630. NADS_GAT. 1 hit.
SUPFAMiSSF56317. SSF56317. 1 hit.
TIGRFAMsiTIGR00552. nadE. 1 hit.
PROSITEiPS50263. CN_HYDROLASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Rat NAD+ synthetase."
    Miura Y., Yamashita N., Suda Y.
    Submitted (AUG-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Entry informationi

Entry nameiNADE_RAT
AccessioniPrimary (citable) accession number: Q812E8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2006
Last sequence update: June 1, 2003
Last modified: May 11, 2016
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.