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Q812D1 (PSIP1_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 80. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
PC4 and SFRS1-interacting protein
Alternative name(s):
Lens epithelium-derived growth factor
Gene names
Name:Psip1
Synonyms:Ledgf
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length528 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Transcriptional coactivator involved in neuroepithelial stem cell differentiation and neurogenesis. Involved in particular in lens epithelial cell gene regulation and stress responses. May play an important role in lens epithelial to fiber cell terminal differentiation. May play a protective role during stress-induced apoptosis By similarity.

Subunit structure

Interacts with IFRD1/PC4. Interacts POGZ and CDCA7L By similarity.

Subcellular location

Nucleus By similarity.

Post-translational modification

Citrullinated by PADI4 By similarity.

Sequence similarities

Belongs to the HDGF family.

Contains 1 PWWP domain.

Ontologies

Keywords
   Biological processTranscription
Transcription regulation
   Cellular componentNucleus
   Coding sequence diversityAlternative splicing
   DomainCoiled coil
   LigandDNA-binding
   PTMCitrullination
Phosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processmRNA 5'-splice site recognition

Inferred from sequence or structural similarity. Source: UniProtKB

positive regulation of transcription from RNA polymerase II promoter

Inferred from sequence or structural similarity. Source: UniProtKB

regulation of transcription, DNA-templated

Inferred from electronic annotation. Source: UniProtKB-KW

response to heat

Inferred from sequence or structural similarity. Source: UniProtKB

response to oxidative stress

Inferred from sequence or structural similarity. Source: UniProtKB

transcription, DNA-templated

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentnuclear heterochromatin

Inferred from direct assay PubMed 21345933. Source: UniProtKB

nuclear periphery

Inferred from sequence or structural similarity. Source: UniProtKB

nucleoplasm

Inferred from sequence or structural similarity. Source: UniProtKB

nucleus

Inferred from sequence or structural similarity. Source: UniProtKB

transcriptionally active chromatin

Inferred from direct assay PubMed 21345933. Source: UniProtKB

   Molecular_functionRNA polymerase II transcription coactivator activity

Inferred from sequence or structural similarity. Source: UniProtKB

activating transcription factor binding

Inferred from sequence or structural similarity. Source: UniProtKB

chromatin binding

Inferred from sequence or structural similarity. Source: UniProtKB

supercoiled DNA binding

Inferred from direct assay PubMed 21345933. Source: UniProtKB

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q812D1-1)

Also known as: Ledgfa;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q812D1-2)

Also known as: Ledgfb;

The sequence of this isoform differs from the canonical sequence as follows:
     324-331: QQTKDEGK → HQTTCNLQ
     332-528: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 528528PC4 and SFRS1-interacting protein
PRO_0000191710

Regions

Domain7 – 6458PWWP
Coiled coil304 – 33229 Potential
Coiled coil369 – 39325 Potential
Motif146 – 15611Nuclear localization signal By similarity

Amino acid modifications

Modified residue1021Phosphoserine By similarity
Modified residue1061Phosphoserine By similarity
Modified residue1221Phosphothreonine By similarity
Modified residue1291Phosphoserine By similarity
Modified residue1411Phosphothreonine By similarity
Modified residue1761Phosphoserine By similarity
Modified residue2051Phosphoserine By similarity
Modified residue2721Phosphoserine By similarity
Modified residue2741Phosphoserine By similarity
Modified residue4321Phosphoserine By similarity
Modified residue4351Phosphothreonine By similarity
Modified residue4411Phosphoserine By similarity
Modified residue5151Citrulline By similarity
Modified residue5201Phosphoserine By similarity
Modified residue5251Phosphothreonine By similarity

Natural variations

Alternative sequence324 – 3318QQTKDEGK → HQTTCNLQ in isoform 2.
VSP_014301
Alternative sequence332 – 528197Missing in isoform 2.
VSP_014302

Experimental info

Sequence conflict2731D → Y in AAP97282. Ref.1
Sequence conflict2911H → N in AAP97282. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 (Ledgfa) [UniParc].

Last modified June 1, 2003. Version 1.
Checksum: 8E0C2887842F6125

FASTA52859,639
        10         20         30         40         50         60 
MTRDFKPGDL IFAKMKGYPH WPARVDEVPD GAVKPPTNKL PIFFFGTHET AFLGPKDIFP 

        70         80         90        100        110        120 
YSENKEKYGK PNKRKGFNEG LWEIDNNPKV KFSSQQVSTK QSNASSDVEA EEKETSVSKE 

       130        140        150        160        170        180 
DTDQEEKASN EDVTKAADIT TPKAARRGRK RKAEKQVDTE EAGVVTAATA SNVKASPKRG 

       190        200        210        220        230        240 
RPAATEVKIP KPRGRPKVVK QPCPSESDMV IDEDKSKKKG PEEKPPKKQL KKEEEGQKEE 

       250        260        270        280        290        300 
EKPRKEPDKK EGKKEVESKR KNLAKPGVTS TSDSEEDDDQ EGEKKRKGGR HFQAAHRRNM 

       310        320        330        340        350        360 
LKGQHEKEAA DRKRKQEEQM ETEQQTKDEG KKPEVKKVEK KRETSMDSRL QRIHAEIKNS 

       370        380        390        400        410        420 
LKIDNLDVNR CIEALDELAS LQVTMQQAQK HTEMITTLKK IRRFKVSQVI MEKSTMLYNK 

       430        440        450        460        470        480 
FKNMFLVGEG DSVITQVLNK SLAEQRQHEE ANKTKDQGKK GPNKKLEKEQ TGTKSLNGGS 

       490        500        510        520 
DAQESNHPQH NGDSAEESKD SREAGSKTKT PGEEREAEVS LKESTLDN 

« Hide

Isoform 2 (Ledgfb) [UniParc].

Checksum: E46592E1BDE22D94
Show »

FASTA33137,389

References

[1]Yu L.
Submitted (JAN-2001) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2).
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF339084 mRNA. Translation: AAO32951.1.
AF372090 mRNA. Translation: AAP97282.1.
RefSeqNP_786941.1. NM_175765.2. [Q812D1-1]
UniGeneRn.93075.

3D structure databases

ProteinModelPortalQ812D1.
SMRQ812D1. Positions 4-87, 345-427.
ModBaseSearch...
MobiDBSearch...

PTM databases

PhosphoSiteQ812D1.

Proteomic databases

PaxDbQ812D1.
PRIDEQ812D1.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID313323.
KEGGrno:313323.
UCSCRGD:631439. rat. [Q812D1-1]

Organism-specific databases

CTD11168.
RGD631439. Psip1.

Phylogenomic databases

eggNOGNOG316844.
HOGENOMHOG000230488.
HOVERGENHBG108300.
InParanoidQ812D1.
PhylomeDBQ812D1.

Gene expression databases

GenevestigatorQ812D1.

Family and domain databases

InterProIPR021567. LEDGF.
IPR000313. PWWP_dom.
IPR017859. Treacle-like_TCS.
[Graphical view]
PfamPF11467. LEDGF. 1 hit.
PF00855. PWWP. 1 hit.
[Graphical view]
PRINTSPR01503. TREACLE.
SMARTSM00293. PWWP. 1 hit.
[Graphical view]
PROSITEPS50812. PWWP. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio665985.
PROQ812D1.

Entry information

Entry namePSIP1_RAT
AccessionPrimary (citable) accession number: Q812D1
Secondary accession number(s): Q7TNY0
Entry history
Integrated into UniProtKB/Swiss-Prot: July 5, 2005
Last sequence update: June 1, 2003
Last modified: May 14, 2014
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families