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Q812A5 (PRR5_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 55. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Proline-rich protein 5
Alternative name(s):
Protein observed with Rictor-1
Short name=Protor-1
Gene names
Name:Prr5
Synonyms:Protor1
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length387 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Subunit of mTORC2, which regulates cell growth and survival in response to hormonal signals. mTORC2 is activated by growth factors, but, in contrast to mTORC1, seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422'. mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657'. PRR5 plays an important role in regulation of PDGFRB expression and in modulation of platelet-derived growth factor signaling. May act as a tumor suppressor in breast cancer By similarity.

Subunit structure

Part of the mammalian target of rapamycin complex 2 (mTORC2) which contains MTOR, MLST8, PRR5, RICTOR, MAPKAP1 and DEPTOR By similarity. Contrary to mTORC1, mTORC2 does not bind to and is not sensitive to FKBP12-rapamycin. Binds directly to MTOR and RICTOR within the TORC2 complex By similarity. Interacts with MTOR. Ref.5

Tissue specificity

Ubiquitously expressed. Expressed at high levels in kidney. Ref.1

Sequence similarities

Belongs to the PROTOR family.

Caution

Was originally thought (Ref.1) to be the sequence of Arhgap8 but is actually the sequence of Prr5.

Sequence caution

The sequence BAC32586.1 differs from that shown. Reason: Frameshift at position 296.

Ontologies

Keywords
   Biological processCell cycle
   Coding sequence diversityAlternative splicing
   DiseaseTumor suppressor
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcell cycle

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 Ref.1 Ref.2 (identifier: Q812A5-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 Ref.2 Ref.3 (identifier: Q812A5-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-9: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 387387Proline-rich protein 5
PRO_0000308163

Regions

Region10 – 9687Interaction with RICTOR By similarity UniProtKB P85299
Region189 – 21931Interaction with RICTOR By similarity UniProtKB P85299

Natural variations

Alternative sequence1 – 99Missing in isoform 2. Ref.2 Ref.3
VSP_052580

Experimental info

Sequence conflict491N → K in BAC38171. Ref.2
Sequence conflict301 – 3033GQG → ATR in AAO39849. Ref.1
Sequence conflict3051S → F in AAO39849. Ref.1
Sequence conflict3401S → P in AAO39849. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified October 23, 2007. Version 2.
Checksum: CDF8984591BE2102

FASTA38742,523
        10         20         30         40         50         60 
MRTLRRLKFM SSPSLSDLGK REPGAAGTDE RGTQQRRACA NATWNSIHNG VIAVFQRKGL 

        70         80         90        100        110        120 
PDQELFILNE GVRQLLKTEL GSFFTEYLQN QLLTKGMVIL RDKIRFYEGQ KLLDSLAETW 

       130        140        150        160        170        180 
DFFFSDVLPT LQAIFYPVQG KEPSVRQLAL LHFRNTITLS VKLEDALARS HARVPPAIAQ 

       190        200        210        220        230        240 
MLLVLQGVHE SRGVTEDYLR LETLIQKVVS PYLGTYGLYS NEGPCTHSCI LEKRFLRRSR 

       250        260        270        280        290        300 
SGDILAKNPV VRSKSYNTPL LNPVAEHEAE GTAASGTSIR RHSVSEMTSC PEPQGFVDTP 

       310        320        330        340        350        360 
GQGPSGTFRS SPTPHSGPCP SRLYPPAHSP EQGPGHGSPS TSSPETLVDQ ILESADSDSE 

       370        380 
GIFIDFGRGS RSSVSDFEAP GGRPSVV

« Hide

Isoform 2 [UniParc].

Checksum: 4856C69398AC9BA8
Show »

FASTA37841,321

References

« Hide 'large scale' references
[1]"Identification and characterization of a gene encoding a putative mouse Rho GTPase activating protein gene 8, Arhgap8."
Shan Z., Haaf T., Popescu N.C.
Gene 303:55-61(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY.
[2]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
Strain: C57BL/6J.
Tissue: Corpora quadrigemina and Corpus striatum.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Strain: FVB/N.
Tissue: Kidney.
[4]"PRR5 encodes a conserved proline-rich protein predominant in kidney: analysis of genomic organization, expression, and mutation status in breast and colorectal carcinomas."
Johnstone C.N., Castellvi-Bel S., Chang L.M., Sung R.K., Bowser M.J., Pique J.M., Castells A., Rustgi A.K.
Genomics 85:338-351(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION.
[5]"Tel2 structure and function in the Hsp90-dependent maturation of mTOR and ATR complexes."
Takai H., Xie Y., de Lange T., Pavletich N.P.
Genes Dev. 24:2019-2030(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH MTOR.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AF482997 mRNA. Translation: AAO39849.1.
AK046057 mRNA. Translation: BAC32586.1. Frameshift.
AK081231 mRNA. Translation: BAC38171.1.
BC024991 mRNA. Translation: AAH24991.1.
BK005634 mRNA. Translation: DAA05653.1.
IPIIPI00330041.
IPI00620749.
RefSeqNP_666173.4. NM_146061.4.
UniGeneMm.291372.

3D structure databases

ProteinModelPortalQ812A5.
ModBaseSearch...

PTM databases

PhosphoSiteQ812A5.

Proteomic databases

PRIDEQ812A5.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000065499; ENSMUSP00000066396; ENSMUSG00000036106.
ENSMUST00000171460; ENSMUSP00000127890; ENSMUSG00000036106.
GeneID109270.
KEGGmmu:109270.
UCSCuc007xcd.1. mouse.
uc007xcf.1. mouse.

Organism-specific databases

CTD55615.
MGIMGI:1924714. Prr5.

Phylogenomic databases

eggNOGNOG46045.
GeneTreeENSGT00530000063981.
HOGENOMHOG000290687.
HOVERGENHBG067971.
InParanoidQ812A5.
OMACILEKRF.
OrthoDBEOG4CNQRX.

Gene expression databases

BgeeQ812A5.
CleanExMM_PRR5.
GenevestigatorQ812A5.

Family and domain databases

InterProIPR013745. HbrB.
[Graphical view]
PfamPF08539. HbrB. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio361861.
SOURCESearch...

Entry information

Entry namePRR5_MOUSE
AccessionPrimary (citable) accession number: Q812A5
Secondary accession number(s): Q5EAK2 expand/collapse secondary AC list , Q8BIE7, Q8BIK1, Q8R1A0
Entry history
Integrated into UniProtKB/Swiss-Prot: October 23, 2007
Last sequence update: October 23, 2007
Last modified: April 3, 2013
This is version 55 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents