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Q811W2 (CP26B_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 109. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cytochrome P450 26B1

EC=1.14.-.-
Alternative name(s):
Cytochrome P450 retinoic acid-inactivating 2
Short name=Cytochrome P450RAI-2
Gene names
Name:Cyp26b1
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length512 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in the metabolism of retinoic acid (RA), rendering this classical morphogen inactive through oxidation. Involved in the specific inactivation of all-trans-retinoic acid (all-trans-RA), with a preference for the following substrates: all-trans-RA > 9-cis-RA > 13-cis-RA. Generates several hydroxylated forms of RA, including 4-OH-RA, 4-oxo-RA, and 18-OH-RA. Esential for postnatal survival. Plays a central role in germ cell development: acts by degrading RA in the developing testis, preventing STRA8 expression, thereby leading to delay of meiosis. Required for the maintenance of the undifferentiated state of male germ cells during embryonic development in Sertoli cells, inducing arrest in G0 phase of the cell cycle and preventing meiotic entry. Plays a role in skeletal development, both at the level of patterning and in the ossification of bone and the establishment of some synovial joints. Ref.6 Ref.7 Ref.8 Ref.9

Cofactor

Heme group By similarity.

Subcellular location

Endoplasmic reticulum membrane; Peripheral membrane protein By similarity. Microsome membrane; Peripheral membrane protein By similarity.

Developmental stage

In the embryonic male gonad, expressed in somatic cells as early as E11.5 and persist throughout development. Expression is detected in peritubular myoepithelial cells in the postnatal testis, while expression is absent in developing and adult ovaries. Ref.6 Ref.7

Disruption phenotype

Germ cells enter meiosis precociously in embryonic testes, due to strong-up-regulation of Stra8. Ref.6

Sequence similarities

Belongs to the cytochrome P450 family.

Ontologies

Keywords
   Cellular componentEndoplasmic reticulum
Membrane
Microsome
   LigandHeme
Iron
Metal-binding
   Molecular functionMonooxygenase
Oxidoreductase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processbone morphogenesis

Inferred from electronic annotation. Source: Ensembl

cell fate determination

Inferred from mutant phenotype Ref.6. Source: MGI

cellular response to retinoic acid

Inferred from expression pattern PubMed 18816858. Source: UniProtKB

cornification

Inferred from mutant phenotype PubMed 22366455. Source: MGI

embryonic limb morphogenesis

Inferred from mutant phenotype PubMed 15030763. Source: MGI

establishment of skin barrier

Inferred from mutant phenotype PubMed 22366455. Source: MGI

male meiosis

Inferred from mutant phenotype Ref.6. Source: MGI

negative regulation of retinoic acid receptor signaling pathway

Inferred from mutant phenotype PubMed 22366455. Source: MGI

positive regulation of gene expression

Inferred from mutant phenotype PubMed 18816858. Source: UniProtKB

positive regulation of tongue muscle cell differentiation

Inferred from mutant phenotype PubMed 18816858. Source: UniProtKB

proximal/distal pattern formation

Inferred from mutant phenotype PubMed 15030763. Source: MGI

retinoic acid catabolic process

Inferred by curator PubMed 22366455. Source: MGI

retinoic acid metabolic process

Inferred by curator PubMed 15030763. Source: MGI

retinoic acid receptor signaling pathway

Inferred from mutant phenotype PubMed 18816858. Source: UniProtKB

spermatogenesis

Inferred from mutant phenotype Ref.6. Source: MGI

tongue morphogenesis

Inferred from mutant phenotype PubMed 18816858. Source: UniProtKB

   Cellular_componentendoplasmic reticulum membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionheme binding

Inferred from electronic annotation. Source: InterPro

iron ion binding

Inferred from electronic annotation. Source: InterPro

retinoic acid 4-hydroxylase activity

Inferred from electronic annotation. Source: Ensembl

retinoic acid binding

Inferred from electronic annotation. Source: Ensembl

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 512512Cytochrome P450 26B1
PRO_0000416905

Sites

Metal binding4411Iron (heme axial ligand) Potential

Experimental info

Sequence conflict67 – 682LQ → TR in BAE38630. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Q811W2 [UniParc].

Last modified June 1, 2003. Version 1.
Checksum: AAA063DE5718824E

FASTA51257,375
        10         20         30         40         50         60 
MLFEGLELVS ALATLAACLV SVTLLLAVSQ QLWQLRWAAT RDKSCKLPIP KGSMGFPLIG 

        70         80         90        100        110        120 
ETGHWLLQGS GFQSSRREKY GNVFKTHLLG RPLIRVTGAE NVRKILLGEH QLVSTEWPRS 

       130        140        150        160        170        180 
ARVLLGPNTV ANSIGDIHRN KRKVFSKIFS HEALESYLPK IQLVIQDTLR AWSSQPEAIN 

       190        200        210        220        230        240 
VYQEAQRLTF RMAVRVLLGF SIPEEDLGHL FEVYQQFVEN VFSLPVDLPF SGYRRGIQAR 

       250        260        270        280        290        300 
QILQKGLEKA IREKLQCTQG KDYSDALDIL IESSKEHGKE MTMQELKDGT LELIFAAYAT 

       310        320        330        340        350        360 
TASASTSLIM QLLKHPAVLE KLREELRAQG LLHGGGCPCE GTLRLDTLSS LRYLDCVIKE 

       370        380        390        400        410        420 
VMRLFTPVSG GYRTVLQTFE LDGFQIPKGW SVMYSIRDTH DTAPVFKDVN VFDPDRFSQA 

       430        440        450        460        470        480 
RSEDKDGRFH YLPFGGGVRT CLGKHLAKLF LKVLAVELAS TSRFELATRT FPRITLVPVL 

       490        500        510 
HPVDGLSVKF FGLDSNQNEI LPETEAMLSA TV 

« Hide

References

« Hide 'large scale' references
[1]"Sexually dimorphic gene expression in the developing mouse gonad."
Menke D.B., Page D.C.
Gene Expr. Patterns 2:359-367(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
Strain: C57BL/6.
Tissue: Testis.
[2]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J.
Tissue: Embryo and Mammary gland.
[3]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[4]Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6.
Tissue: Brain.
[6]"Retinoid signaling determines germ cell fate in mice."
Bowles J., Knight D., Smith C., Wilhelm D., Richman J., Mamiya S., Yashiro K., Chawengsaksophak K., Wilson M.J., Rossant J., Hamada H., Koopman P.
Science 312:596-600(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, DISRUPTION PHENOTYPE, DEVELOPMENTAL STAGE.
[7]"Retinoic acid regulates sex-specific timing of meiotic initiation in mice."
Koubova J., Menke D.B., Zhou Q., Capel B., Griswold M.D., Page D.C.
Proc. Natl. Acad. Sci. U.S.A. 103:2474-2479(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, DEVELOPMENTAL STAGE.
[8]"Cyp26b1 expression in murine Sertoli cells is required to maintain male germ cells in an undifferentiated state during embryogenesis."
Li H., MacLean G., Cameron D., Clagett-Dame M., Petkovich M.
PLoS ONE 4:E7501-E7501(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[9]"Craniosynostosis and multiple skeletal anomalies in humans and zebrafish result from a defect in the localized degradation of retinoic acid."
Laue K., Pogoda H.M., Daniel P.B., van Haeringen A., Alanay Y., von Ameln S., Rachwalski M., Morgan T., Gray M.J., Breuning M.H., Sawyer G.M., Sutherland-Smith A.J., Nikkels P.G., Kubisch C., Bloch W., Wollnik B., Hammerschmidt M., Robertson S.P.
Am. J. Hum. Genet. 89:595-606(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION IN SKELETAL DEVELOPMENT.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY134662 mRNA. Translation: AAN08613.1.
AK137124 mRNA. Translation: BAE23242.1.
AK166211 mRNA. Translation: BAE38630.1.
AC153606 Genomic DNA. No translation available.
CH466523 Genomic DNA. Translation: EDK99117.1.
BC059246 mRNA. Translation: AAH59246.1.
RefSeqNP_001171184.1. NM_001177713.1.
NP_780684.1. NM_175475.3.
UniGeneMm.255246.

3D structure databases

ProteinModelPortalQ811W2.
SMRQ811W2. Positions 47-493.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10090.ENSMUSP00000076886.

PTM databases

PhosphoSiteQ811W2.

Proteomic databases

PRIDEQ811W2.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000077705; ENSMUSP00000076886; ENSMUSG00000063415.
ENSMUST00000168003; ENSMUSP00000128391; ENSMUSG00000063415.
GeneID232174.
KEGGmmu:232174.
UCSCuc009coy.2. mouse.

Organism-specific databases

CTD56603.
MGIMGI:2176159. Cyp26b1.

Phylogenomic databases

GeneTreeENSGT00660000095370.
HOGENOMHOG000220829.
HOVERGENHBG051099.
InParanoidQ811W2.
KOK12664.
OMAGIQARQT.
OrthoDBEOG7F24SP.
PhylomeDBQ811W2.
TreeFamTF105093.

Gene expression databases

BgeeQ811W2.
GenevestigatorQ811W2.

Family and domain databases

Gene3D1.10.630.10. 1 hit.
InterProIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002403. Cyt_P450_E_grp-IV.
[Graphical view]
PfamPF00067. p450. 1 hit.
[Graphical view]
PRINTSPR00465. EP450IV.
PR00385. P450.
SUPFAMSSF48264. SSF48264. 1 hit.
PROSITEPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio380970.
PROQ811W2.
SOURCESearch...

Entry information

Entry nameCP26B_MOUSE
AccessionPrimary (citable) accession number: Q811W2
Secondary accession number(s): Q3TM12
Entry history
Integrated into UniProtKB/Swiss-Prot: April 18, 2012
Last sequence update: June 1, 2003
Last modified: April 16, 2014
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot