Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

ELKS/Rab6-interacting/CAST family member 1

Gene

Erc1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Regulatory subunit of the IKK complex. Probably recruits IkappaBalpha/NFKBIA to the complex (By similarity). May be involved in the organization of the cytomatrix at the nerve terminals active zone (CAZ) which regulates neurotransmitter release. May be involved in vesicle trafficking at the CAZ. May be involved in Rab-6 regulated endosomes to Golgi transport.By similarity

GO - Molecular functioni

  • PDZ domain binding Source: RGD
  • Rab GTPase binding Source: ParkinsonsUK-UCL

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
ELKS/Rab6-interacting/CAST family member 1
Short name:
ERC-1
Alternative name(s):
CAZ-associated structural protein 2
Short name:
CAST2
Rab6-interacting protein 2
Gene namesi
Name:Erc1
Synonyms:Cast2, Elks, Rab6ip2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi628733. Erc1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Golgi apparatus, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi943 – 9431E → K or A: No effect on RIMS1 and RIMS2 binding.
Mutagenesisi944 – 9441E → K or A: No effect on RIMS1 and RIMS2 binding.
Mutagenesisi945 – 9451G → D or A: Abolishes RIMS1 and RIMS2 binding.
Mutagenesisi946 – 9461I → D or A: Abolishes RIMS1 and RIMS2 binding.
Mutagenesisi947 – 9471W → D or A: Abolishes RIMS1 and RIMS2 binding.
Mutagenesisi948 – 9481A → D: Abolishes RIMS1 and RIMS2 binding.
Mutagenesisi948 – 9481A → L: Weakens RIMS1 and abolishes RIMS2 binding.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 948948ELKS/Rab6-interacting/CAST family member 1PRO_0000097178Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei10 – 101N6-acetyllysineBy similarity
Modified residuei17 – 171PhosphoserineBy similarity
Modified residuei21 – 211PhosphoserineBy similarity
Modified residuei37 – 371PhosphoserineBy similarity
Modified residuei38 – 381PhosphothreonineBy similarity
Modified residuei55 – 551PhosphoserineBy similarity
Modified residuei75 – 751PhosphoserineBy similarity
Modified residuei94 – 941PhosphoserineBy similarity
Modified residuei796 – 7961PhosphoserineBy similarity
Modified residuei937 – 9371PhosphoserineCombined sources

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ811U3.
PRIDEiQ811U3.

PTM databases

iPTMnetiQ811U3.
PhosphoSiteiQ811U3.

Expressioni

Tissue specificityi

Isoform 1 is specifically expressed in brain. A further probable isoform is widely expressed outside of brain It is referred to as ERC1a by PubMed:12391317 and characterized by a C-terminus identical to that of isoforms 1 in human and mouse.1 Publication

Interactioni

Subunit structurei

Interacts with the GTB-bound forms of RAB6A isoform 1 and isoform 2 and with RAB6B. The interaction was strongest with RAB6B, followed by RAB6A isoform 2 and weakest with RAB6A isoform 1 (By similarity). Part of a complex with CHUK, IKBKB and IKBKG. Interacts with CHUK, IKBKB and IKBKG. The interaction with IKBKG is independent of CHUK and IKBKB. Interacts with NFKBIA (By similarity). Isoform 1 interacts through its C-terminus with the PDZ domains of RIMS1 and RIMS2. Interacts with ERC2/CAST1.By similarity3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Rims1Q9JIR48EBI-3507502,EBI-3507436

GO - Molecular functioni

  • PDZ domain binding Source: RGD
  • Rab GTPase binding Source: ParkinsonsUK-UCL

Protein-protein interaction databases

IntActiQ811U3. 3 interactions.
MINTiMINT-1784084.
STRINGi10116.ENSRNOP00000012397.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1ZUBNMR-B939-948[»]
ProteinModelPortaliQ811U3.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ811U3.

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili144 – 920777Sequence analysisAdd
BLAST

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG4809. Eukaryota.
ENOG410Y4GU. LUCA.
HOGENOMiHOG000236353.
HOVERGENiHBG051496.
InParanoidiQ811U3.
KOiK16072.

Family and domain databases

InterProiIPR019323. ELKS/CAST.
[Graphical view]
PANTHERiPTHR18861. PTHR18861. 1 hit.
PfamiPF10174. Cast. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: Additional isoforms seem to exist.
Isoform 1 (identifier: Q811U3-1) [UniParc]FASTAAdd to basket
Also known as: CAST2a, ERC1b

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MYGSARSVGK VEPSSQSPGR SPRLPRSPRL GHRRTNSTGG SSGNSVGGGS
60 70 80 90 100
GKTLSMENIQ SLNAAYATSG PMYLSDHENV GAETPKSTMT LGRSGGRLPY
110 120 130 140 150
GVRMTAMGSS PNIASSGVAS DTIAFGEHHL PPVSMASTVP HSLRQARDNT
160 170 180 190 200
IMDLQTQLKE VLRENDLLRK DVEVKESKLS SSMNSIKTFW SPELKKERAL
210 220 230 240 250
RKDEASKITI WKEQYRVVQE ENQHMQMTIQ ALQDELRIQR DLNQLFQQDS
260 270 280 290 300
SSRTGEPCVA ELTEENFQRL HAEHERQAKE LFLLRKTLEE MELRIETQKQ
310 320 330 340 350
TLNARDESIK KLLEMLQSKG LSAKATEEDH ERTRRLAEAE MHVHHLESLL
360 370 380 390 400
EQKEKENNML REEMHRRFEN APDSAKTKAL QTVIEMKDSK ISSMERGLRD
410 420 430 440 450
LEEEIQMLKS NGALSTEERE EEMKQMEVYR SHSKFMKNKI GQVKQELSRK
460 470 480 490 500
DTELLALQTK LETLTNQFSD SKQHIEVLKE SLTAKEQRAA ILQTEVDALR
510 520 530 540 550
LRLEEKETML NKKTKQIQDM AEEKGTQAGE IHDLKDMLDV KERKVNVLQK
560 570 580 590 600
KIENLQEQLR DKEKQMSSLK ERVKSLQADT TNTDTALTTL EEALADKERT
610 620 630 640 650
IERLKEQRDR DEREKQEEID TYKKDLKDLK EKVSLLQGDL SEKEASLLDL
660 670 680 690 700
KEHASSLASS GLKKDSRLKT LEIALEQKKE ECLKMESQLK KAHEATLEAR
710 720 730 740 750
ASPEMSDRIQ QLEREIARYK DESSKAQTEV DRLLEILKEV ENEKNDKDKK
760 770 780 790 800
IAELESLTSR QVKDQNKKVA NLKHKEQVEK KKSAQMLEEA RRREDSLSDS
810 820 830 840 850
SQQLQVEELL MAMEKVKQEL ESMKAKLSST QQSLAEKETH LTNLRAERRK
860 870 880 890 900
HLEEVLEMKQ EALLAAISEK DANIALLELS SSKKKTQEEV AALKREKDRL
910 920 930 940
VQQLKQQTQN RMKLMADNYE DDHFRSSRSN QTNHKPSPDQ DEEEGIWA
Length:948
Mass (Da):108,818
Last modified:June 1, 2003 - v1
Checksum:i7CC15D7B1939561C
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti771 – 7711N → S in AAN39293 (PubMed:12391317).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY174115 mRNA. Translation: AAO25554.1.
AF541926 mRNA. Translation: AAN39293.1.
RefSeqiNP_740769.2. NM_170788.2. [Q811U3-1]
UniGeneiRn.207836.

Genome annotation databases

GeneIDi266806.
KEGGirno:266806.
UCSCiRGD:628733. rat. [Q811U3-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY174115 mRNA. Translation: AAO25554.1.
AF541926 mRNA. Translation: AAN39293.1.
RefSeqiNP_740769.2. NM_170788.2. [Q811U3-1]
UniGeneiRn.207836.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1ZUBNMR-B939-948[»]
ProteinModelPortaliQ811U3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ811U3. 3 interactions.
MINTiMINT-1784084.
STRINGi10116.ENSRNOP00000012397.

PTM databases

iPTMnetiQ811U3.
PhosphoSiteiQ811U3.

Proteomic databases

PaxDbiQ811U3.
PRIDEiQ811U3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi266806.
KEGGirno:266806.
UCSCiRGD:628733. rat. [Q811U3-1]

Organism-specific databases

CTDi23085.
RGDi628733. Erc1.

Phylogenomic databases

eggNOGiKOG4809. Eukaryota.
ENOG410Y4GU. LUCA.
HOGENOMiHOG000236353.
HOVERGENiHBG051496.
InParanoidiQ811U3.
KOiK16072.

Miscellaneous databases

EvolutionaryTraceiQ811U3.
PROiQ811U3.

Family and domain databases

InterProiIPR019323. ELKS/CAST.
[Graphical view]
PANTHERiPTHR18861. PTHR18861. 1 hit.
PfamiPF10174. Cast. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRB6I2_RAT
AccessioniPrimary (citable) accession number: Q811U3
Secondary accession number(s): Q8CIY9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 31, 2004
Last sequence update: June 1, 2003
Last modified: July 6, 2016
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.