Q811U3 (RB6I2_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
ELKS/Rab6-interacting/CAST family member 1
Short name=ERC-1

Alternative name(s):
CAZ-associated structural protein 2
Short name=CAST2
Rab6-interacting protein 2

Gene names

Name:	Erc1
Synonyms:	Cast2, Elks, Rab6ip2

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	948 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Regulatory subunit of the IKK complex. Probably recruits IkappaBalpha/NFKBIA to the complex By similarity. May be involved in the organization of the cytomatrix at the nerve terminals active zone (CAZ) which regulates neurotransmitter release. May be involved in vesicle trafficking at the CAZ. May be involved in Rab-6 regulated endosomes to Golgi transport.
Subunit structure	Interacts with the GTB-bound forms of RAB6A isoform 1 and isoform 2 and with RAB6B. The interaction was strongest with RAB6B, followed by RAB6A isoform 2 and weakest with RAB6A isoform 1 By similarity. Part of a complex with CHUK, IKBKB and IKBKG. Interacts with CHUK, IKBKB and IKBKG. The interaction with IKBKG is independent of CHUK and IKBKB. Interacts with NFKBIA By similarity. Isoform 1 interacts through its C-terminus with the PDZ domains of RIMS1 and RIMS2. Interacts with ERC2/CAST1. Ref.1 Ref.2 Ref.3
Subcellular location	Cytoplasm By similarity. Membrane; Peripheral membrane protein By similarity. Golgi apparatus membrane; Peripheral membrane protein By similarity. Note: In neurons, isoform 1 is localized to the cytoplasm and to synapses where it is detected in presynaptic nerve terminals close to the active zone. Recruited on Golgi membranes by RAB6A in a GTP-dependent manner By similarity. Ref.2
Tissue specificity	Isoform 1 is specifically expressed in brain. A further probable isoform is widely expressed outside of brain It is referred to as ERC1a by Ref.2 and characterized by a C-terminus identical to that of isoforms 1 in human and mouse. Ref.2

Ontologies

Keywords
Biological process	Protein transport Transport
Cellular component	Cytoplasm Golgi apparatus Membrane
Coding sequence diversity	Alternative splicing
Domain	Coiled coil
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	protein transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	PDZ domain binding Inferred from direct assay Ref.2. Source: RGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
Rims1	Q9JIR4	3	EBI-3507502,EBI-3507436

Alternative products

This entry describes 1 isoform produced by alternative splicing. [Select]

Note: Additional isoforms seem to exist.

Isoform 1 (identifier: Q811U3-1)

Also known as: CAST2a; ERC1b;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 948	948	ELKS/Rab6-interacting/CAST family member 1		PRO_0000097178
Regions
	Coiled coil	144 – 920	777	Potential
Amino acid modifications
	Modified residue	17	1	Phosphoserine By similarity
	Modified residue	21	1	Phosphoserine By similarity
	Modified residue	37	1	Phosphoserine By similarity
	Modified residue	75	1	Phosphoserine By similarity
	Modified residue	94	1	Phosphoserine By similarity
	Modified residue	252	1	Phosphoserine By similarity
Experimental info
	Mutagenesis	943	1	E → K or A: No effect on RIMS1 and RIMS2 binding.
	Mutagenesis	944	1	E → K or A: No effect on RIMS1 and RIMS2 binding.
	Mutagenesis	945	1	G → D or A: Abolishes RIMS1 and RIMS2 binding.
	Mutagenesis	946	1	I → D or A: Abolishes RIMS1 and RIMS2 binding.
	Mutagenesis	947	1	W → D or A: Abolishes RIMS1 and RIMS2 binding.
	Mutagenesis	948	1	A → D: Abolishes RIMS1 and RIMS2 binding.
	Mutagenesis	948	1	A → L: Weakens RIMS1 and abolishes RIMS2 binding.
	Sequence conflict	771	1	N → S in AAN39293. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (CAST2a) (ERC1b) [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 7CC15D7B1939561C	FASTA	948	108,818
	10 20 30 40 50 60 MYGSARSVGK VEPSSQSPGR SPRLPRSPRL GHRRTNSTGG SSGNSVGGGS GKTLSMENIQ 70 80 90 100 110 120 SLNAAYATSG PMYLSDHENV GAETPKSTMT LGRSGGRLPY GVRMTAMGSS PNIASSGVAS 130 140 150 160 170 180 DTIAFGEHHL PPVSMASTVP HSLRQARDNT IMDLQTQLKE VLRENDLLRK DVEVKESKLS 190 200 210 220 230 240 SSMNSIKTFW SPELKKERAL RKDEASKITI WKEQYRVVQE ENQHMQMTIQ ALQDELRIQR 250 260 270 280 290 300 DLNQLFQQDS SSRTGEPCVA ELTEENFQRL HAEHERQAKE LFLLRKTLEE MELRIETQKQ 310 320 330 340 350 360 TLNARDESIK KLLEMLQSKG LSAKATEEDH ERTRRLAEAE MHVHHLESLL EQKEKENNML 370 380 390 400 410 420 REEMHRRFEN APDSAKTKAL QTVIEMKDSK ISSMERGLRD LEEEIQMLKS NGALSTEERE 430 440 450 460 470 480 EEMKQMEVYR SHSKFMKNKI GQVKQELSRK DTELLALQTK LETLTNQFSD SKQHIEVLKE 490 500 510 520 530 540 SLTAKEQRAA ILQTEVDALR LRLEEKETML NKKTKQIQDM AEEKGTQAGE IHDLKDMLDV 550 560 570 580 590 600 KERKVNVLQK KIENLQEQLR DKEKQMSSLK ERVKSLQADT TNTDTALTTL EEALADKERT 610 620 630 640 650 660 IERLKEQRDR DEREKQEEID TYKKDLKDLK EKVSLLQGDL SEKEASLLDL KEHASSLASS 670 680 690 700 710 720 GLKKDSRLKT LEIALEQKKE ECLKMESQLK KAHEATLEAR ASPEMSDRIQ QLEREIARYK 730 740 750 760 770 780 DESSKAQTEV DRLLEILKEV ENEKNDKDKK IAELESLTSR QVKDQNKKVA NLKHKEQVEK 790 800 810 820 830 840 KKSAQMLEEA RRREDSLSDS SQQLQVEELL MAMEKVKQEL ESMKAKLSST QQSLAEKETH 850 860 870 880 890 900 LTNLRAERRK HLEEVLEMKQ EALLAAISEK DANIALLELS SSKKKTQEEV AALKREKDRL 910 920 930 940 VQQLKQQTQN RMKLMADNYE DDHFRSSRSN QTNHKPSPDQ DEEEGIWA

References

[1]	"CAST2: identification and characterization of a protein structurally related to the presynaptic cytomatrix protein CAST." Deguchi-Tawarada M., Inoue E., Takao-Rikitsu E., Inoue M., Ohtsuka T., Takai Y. Genes Cells 9:15-23(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], INTERACTION WITH RIMS1 AND CAST1. Strain: Sprague-Dawley.
[2]	"A family of RIM-binding proteins regulated by alternative splicing: Implications for the genesis of synaptic active zones." Wang Y., Liu X., Biederer T., Suedhof T.C. Proc. Natl. Acad. Sci. U.S.A. 99:14464-14469(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], ALTERNATIVE SPLICING, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, INTERACTION WITH RIMS1 AND RIMS2, MUTAGENESIS OF 943-GLU--ALA-948.
[3]	"Solution structure of the RIM1alpha PDZ domain in complex with an ELKS1b C-terminal peptide." Lu J., Li H., Wang Y., Sudhof T.C., Rizo J. J. Mol. Biol. 352:455-466(2005) [PubMed] [Europe PMC] [Abstract] Cited for: STRUCTURE BY NMR OF 939-948 IN COMPLEX WITH RIMS1, INTERACTION WITH RIMS1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AY174115 mRNA. Translation: AAO25554.1.
AF541926 mRNA. Translation: AAN39293.1.

IPI

IPI00201791.

RefSeq

NP_740769.2. NM_170788.2.

UniGene

Rn.207836.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1ZUB	NMR	-	B	939-948	[»]

ProteinModelPortal

Q811U3.

ModBase

Search...

Protein-protein interaction databases

IntAct

Q811U3. 1 interaction.

MINT

MINT-1784084.

STRING

10116.ENSRNOP00000046625.

PTM databases

PhosphoSite

Q811U3.

Proteomic databases

PaxDb

Q811U3.

PRIDE

Q811U3.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

GeneID

266806.

KEGG

rno:266806.

UCSC

RGD:628733. rat.

Organism-specific databases

CTD

23085.

RGD

628733. Erc1.

Phylogenomic databases

eggNOG

NOG12793.

HOGENOM

HOG000236353.

HOVERGEN

HBG051496.

KO

K16072.

Gene expression databases

ArrayExpress

Q811U3.

Genevestigator

Q811U3.

GermOnline

ENSRNOG00000009264. Rattus norvegicus.

Family and domain databases

InterPro

IPR019323. CAZ_cplx_RIM-bd_prot.
[Graphical view]

Pfam

PF10174. Cast. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

Q811U3.

NextBio

624721.

Entry information

Entry name

RB6I2_RAT

Accession

Primary (citable) accession number: Q811U3
Secondary accession number(s): Q8CIY9

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 31, 2004
Last sequence update:	June 1, 2003
Last modified:	April 3, 2013
This is version 78 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program