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Q811Q9 (PCY1B_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 84. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Choline-phosphate cytidylyltransferase B

EC=2.7.7.15
Alternative name(s):
CCT-beta
CTP:phosphocholine cytidylyltransferase B
Short name=CCT B
Short name=CT B
Phosphorylcholine transferase B
Gene names
Name:Pcyt1b
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length369 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Controls phosphatidylcholine synthesis. Ref.1 Ref.4

Catalytic activity

CTP + phosphocholine = diphosphate + CDP-choline.

Pathway

Phospholipid metabolism; phosphatidylcholine biosynthesis; phosphatidylcholine from phosphocholine: step 1/2.

Subcellular location

Endoplasmic reticulum By similarity.

Tissue specificity

Highly expressed in brain (at protein level). Expressed at lower levels in lung and gonads. Ref.1 Ref.4

Developmental stage

Expression is low at E7, reaches a maximum at E15 and decreases at E17. Ref.1

Disruption phenotype

Female mice lacking isoform 1 have reduced fecundity due to failure in ovary maturation. Male mice lacking isoform 1 have reduced fecundity due to testicular degeneration. Ref.4

Sequence similarities

Belongs to the cytidylyltransferase family.

Sequence caution

The sequence BAC27658.1 differs from that shown. Reason: Erroneous initiation.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q811Q9-1)

Also known as: CCTbeta2;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q811Q9-2)

Also known as: CCTbeta3;

The sequence of this isoform differs from the canonical sequence as follows:
     1-24: Missing.
     25-39: ETMEEIEHTCPQPRL → MDKDEFSRK
Note: Enzymatic activity identical to isoform 1. Not expressed in embryo. Expression starts at 5 weeks.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 369369Choline-phosphate cytidylyltransferase B
PRO_0000247762

Regions

Nucleotide binding84 – 929CTP By similarity
Nucleotide binding168 – 1692CTP By similarity
Nucleotide binding196 – 2005CTP By similarity

Sites

Binding site1221CTP By similarity
Binding site1221Substrate By similarity
Binding site1511Substrate By similarity
Binding site1731CTP By similarity

Amino acid modifications

Modified residue3151Phosphoserine Ref.5
Modified residue3191Phosphoserine Ref.5
Modified residue3621Phosphoserine By similarity

Natural variations

Alternative sequence1 – 2424Missing in isoform 2.
VSP_020041
Alternative sequence25 – 3915ETMEE…PQPRL → MDKDEFSRK in isoform 2.
VSP_020042

Experimental info

Sequence conflict731V → G in AAO39004. Ref.1
Sequence conflict731V → G in AAO39005. Ref.1
Sequence conflict771V → I in BAE28801. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 (CCTbeta2) [UniParc].

Last modified July 25, 2006. Version 2.
Checksum: 841B0E2E9EE22615

FASTA36941,900
        10         20         30         40         50         60 
MPVLTTDAES ETGIPKSLSN EPPSETMEEI EHTCPQPRLT LTAPAPFADE SSCQCQAPHE 

        70         80         90        100        110        120 
KLTVAQARLG TPVDRPVRVY ADGIFDLFHS GHARALMQAK TLFPNSYLLV GVCSDDLTHK 

       130        140        150        160        170        180 
FKGFTVMNEA ERYEALRHCR YVDEVIRDAP WTLTPEFLEK HKIDFVAHDD IPYSSAGSDD 

       190        200        210        220        230        240 
VYKHIKEAGM FVPTQRTEGI STSDIITRIV RDYDVYARRN LQRGYTAKEL NVSFINEKKY 

       250        260        270        280        290        300 
RFQNQVDKMK EKVKNVEERS KEFVNRVEEK SHDLIQKWEE KSREFIGNFL ELFGPDGAWK 

       310        320        330        340        350        360 
QMFQERSSRM LQALSPKQSP VSSPTRSRSP SRSPSPTFSW LPNKTSPPSS PKAASASISS 


MSEGDEDEK 

« Hide

Isoform 2 (CCTbeta3) [UniParc].

Checksum: 2B02AF5565877EBE
Show »

FASTA33938,760

References

« Hide 'large scale' references
[1]"Gene structure, expression and identification of a new CTP:phosphocholine cytidylyltransferase beta isoform."
Karim M., Jackson P., Jackowski S.
Biochim. Biophys. Acta 1633:1-12(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, FUNCTION.
Strain: BALB/c.
Tissue: Brain.
[2]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 18-369 (ISOFORM 1).
Strain: C57BL/6J.
Tissue: Eye, Medulla oblongata and Retina.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Olfactory epithelium.
[4]"Disruption of CCTbeta2 expression leads to gonadal dysfunction."
Jackowski S., Rehg J.E., Zhang Y.-M., Wang J., Miller K., Jackson P., Karim M.A.
Mol. Cell. Biol. 24:4720-4733(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.
[5]"Phosphoproteomic analysis of the developing mouse brain."
Ballif B.A., Villen J., Beausoleil S.A., Schwartz D., Gygi S.P.
Mol. Cell. Proteomics 3:1093-1101(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-315 AND SER-319, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Embryonic brain.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY189949 mRNA. Translation: AAO39004.1.
AY189950 mRNA. Translation: AAO39005.1.
AK032027 mRNA. Translation: BAC27658.1. Different initiation.
AK053577 mRNA. Translation: BAC35435.1.
AK149304 mRNA. Translation: BAE28801.1.
BC048917 mRNA. Translation: AAH48917.1.
CCDSCCDS30274.1. [Q811Q9-1]
CCDS41061.1. [Q811Q9-2]
RefSeqNP_808214.1. NM_177546.2. [Q811Q9-2]
NP_997593.1. NM_211138.1. [Q811Q9-1]
UniGeneMm.166467.

3D structure databases

ProteinModelPortalQ811Q9.
SMRQ811Q9. Positions 41-295.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid231814. 1 interaction.

Proteomic databases

PaxDbQ811Q9.
PRIDEQ811Q9.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000045898; ENSMUSP00000044280; ENSMUSG00000035246. [Q811Q9-1]
ENSMUST00000113933; ENSMUSP00000109566; ENSMUSG00000035246. [Q811Q9-2]
GeneID236899.
KEGGmmu:236899.
UCSCuc009tsw.1. mouse. [Q811Q9-1]

Organism-specific databases

CTD9468.
MGIMGI:2147987. Pcyt1b.

Phylogenomic databases

eggNOGCOG0615.
GeneTreeENSGT00390000000269.
HOGENOMHOG000230945.
HOVERGENHBG053531.
InParanoidQ811Q9.
KOK00968.
OMALEHTCPH.
OrthoDBEOG7X9G6S.
PhylomeDBQ811Q9.
TreeFamTF106336.

Enzyme and pathway databases

UniPathwayUPA00753; UER00739.

Gene expression databases

BgeeQ811Q9.
GenevestigatorQ811Q9.

Family and domain databases

Gene3D3.40.50.620. 1 hit.
InterProIPR004821. Cyt_trans-like.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamPF01467. CTP_transf_2. 1 hit.
[Graphical view]
TIGRFAMsTIGR00125. cyt_tran_rel. 1 hit.
ProtoNetSearch...

Other

NextBio383139.
PROQ811Q9.
SOURCESearch...

Entry information

Entry namePCY1B_MOUSE
AccessionPrimary (citable) accession number: Q811Q9
Secondary accession number(s): Q3UEW0 expand/collapse secondary AC list , Q80Y63, Q811Q8, Q8BKD2, Q8C085
Entry history
Integrated into UniProtKB/Swiss-Prot: July 25, 2006
Last sequence update: July 25, 2006
Last modified: July 9, 2014
This is version 84 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot