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Q811Q9

- PCY1B_MOUSE

UniProt

Q811Q9 - PCY1B_MOUSE

Protein

Choline-phosphate cytidylyltransferase B

Gene

Pcyt1b

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 86 (01 Oct 2014)
      Sequence version 2 (25 Jul 2006)
      Previous versions | rss
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    Functioni

    Controls phosphatidylcholine synthesis.2 Publications

    Catalytic activityi

    CTP + phosphocholine = diphosphate + CDP-choline.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei122 – 1221CTPBy similarity
    Binding sitei122 – 1221SubstrateBy similarity
    Binding sitei151 – 1511SubstrateBy similarity
    Binding sitei173 – 1731CTPBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi84 – 929CTPBy similarity
    Nucleotide bindingi168 – 1692CTPBy similarity
    Nucleotide bindingi196 – 2005CTPBy similarity

    GO - Molecular functioni

    1. choline-phosphate cytidylyltransferase activity Source: UniProtKB-EC

    GO - Biological processi

    1. ovarian follicle development Source: MGI
    2. spermatogenesis Source: MGI

    Keywords - Molecular functioni

    Nucleotidyltransferase, Transferase

    Keywords - Biological processi

    Lipid biosynthesis, Lipid metabolism, Phospholipid biosynthesis, Phospholipid metabolism

    Enzyme and pathway databases

    ReactomeiREACT_207335. Synthesis of PC.
    UniPathwayiUPA00753; UER00739.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Choline-phosphate cytidylyltransferase B (EC:2.7.7.15)
    Alternative name(s):
    CCT-beta
    CTP:phosphocholine cytidylyltransferase B
    Short name:
    CCT B
    Short name:
    CT B
    Phosphorylcholine transferase B
    Gene namesi
    Name:Pcyt1b
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome X

    Organism-specific databases

    MGIiMGI:2147987. Pcyt1b.

    Subcellular locationi

    Endoplasmic reticulum By similarity

    GO - Cellular componenti

    1. endoplasmic reticulum Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Endoplasmic reticulum

    Pathology & Biotechi

    Disruption phenotypei

    Female mice lacking isoform 1 have reduced fecundity due to failure in ovary maturation. Male mice lacking isoform 1 have reduced fecundity due to testicular degeneration.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 369369Choline-phosphate cytidylyltransferase BPRO_0000247762Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei315 – 3151Phosphoserine1 Publication
    Modified residuei319 – 3191Phosphoserine1 Publication
    Modified residuei362 – 3621PhosphoserineBy similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiQ811Q9.
    PRIDEiQ811Q9.

    Expressioni

    Tissue specificityi

    Highly expressed in brain (at protein level). Expressed at lower levels in lung and gonads.2 Publications

    Developmental stagei

    Expression is low at E7, reaches a maximum at E15 and decreases at E17.1 Publication

    Gene expression databases

    BgeeiQ811Q9.
    GenevestigatoriQ811Q9.

    Interactioni

    Protein-protein interaction databases

    BioGridi231814. 1 interaction.

    Structurei

    3D structure databases

    ProteinModelPortaliQ811Q9.
    SMRiQ811Q9. Positions 41-295.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the cytidylyltransferase family.Curated

    Phylogenomic databases

    eggNOGiCOG0615.
    GeneTreeiENSGT00390000000269.
    HOGENOMiHOG000230945.
    HOVERGENiHBG053531.
    InParanoidiQ811Q9.
    KOiK00968.
    OMAiLEHTCPH.
    OrthoDBiEOG7X9G6S.
    PhylomeDBiQ811Q9.
    TreeFamiTF106336.

    Family and domain databases

    Gene3Di3.40.50.620. 1 hit.
    InterProiIPR004821. Cyt_trans-like.
    IPR014729. Rossmann-like_a/b/a_fold.
    [Graphical view]
    PfamiPF01467. CTP_transf_2. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00125. cyt_tran_rel. 1 hit.

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q811Q9-1) [UniParc]FASTAAdd to Basket

    Also known as: CCTbeta2

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MPVLTTDAES ETGIPKSLSN EPPSETMEEI EHTCPQPRLT LTAPAPFADE    50
    SSCQCQAPHE KLTVAQARLG TPVDRPVRVY ADGIFDLFHS GHARALMQAK 100
    TLFPNSYLLV GVCSDDLTHK FKGFTVMNEA ERYEALRHCR YVDEVIRDAP 150
    WTLTPEFLEK HKIDFVAHDD IPYSSAGSDD VYKHIKEAGM FVPTQRTEGI 200
    STSDIITRIV RDYDVYARRN LQRGYTAKEL NVSFINEKKY RFQNQVDKMK 250
    EKVKNVEERS KEFVNRVEEK SHDLIQKWEE KSREFIGNFL ELFGPDGAWK 300
    QMFQERSSRM LQALSPKQSP VSSPTRSRSP SRSPSPTFSW LPNKTSPPSS 350
    PKAASASISS MSEGDEDEK 369
    Length:369
    Mass (Da):41,900
    Last modified:July 25, 2006 - v2
    Checksum:i841B0E2E9EE22615
    GO
    Isoform 2 (identifier: Q811Q9-2) [UniParc]FASTAAdd to Basket

    Also known as: CCTbeta3

    The sequence of this isoform differs from the canonical sequence as follows:
         1-24: Missing.
         25-39: ETMEEIEHTCPQPRL → MDKDEFSRK

    Note: Enzymatic activity identical to isoform 1. Not expressed in embryo. Expression starts at 5 weeks.

    Show »
    Length:339
    Mass (Da):38,760
    Checksum:i2B02AF5565877EBE
    GO

    Sequence cautioni

    The sequence BAC27658.1 differs from that shown. Reason: Erroneous initiation.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti73 – 731V → G in AAO39004. (PubMed:12842190)Curated
    Sequence conflicti73 – 731V → G in AAO39005. (PubMed:12842190)Curated
    Sequence conflicti77 – 771V → I in BAE28801. (PubMed:16141072)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 2424Missing in isoform 2. 2 PublicationsVSP_020041Add
    BLAST
    Alternative sequencei25 – 3915ETMEE…PQPRL → MDKDEFSRK in isoform 2. 2 PublicationsVSP_020042Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY189949 mRNA. Translation: AAO39004.1.
    AY189950 mRNA. Translation: AAO39005.1.
    AK032027 mRNA. Translation: BAC27658.1. Different initiation.
    AK053577 mRNA. Translation: BAC35435.1.
    AK149304 mRNA. Translation: BAE28801.1.
    BC048917 mRNA. Translation: AAH48917.1.
    CCDSiCCDS30274.1. [Q811Q9-1]
    CCDS41061.1. [Q811Q9-2]
    RefSeqiNP_808214.1. NM_177546.2. [Q811Q9-2]
    NP_997593.1. NM_211138.1. [Q811Q9-1]
    UniGeneiMm.166467.

    Genome annotation databases

    EnsembliENSMUST00000045898; ENSMUSP00000044280; ENSMUSG00000035246. [Q811Q9-1]
    ENSMUST00000113933; ENSMUSP00000109566; ENSMUSG00000035246. [Q811Q9-2]
    GeneIDi236899.
    KEGGimmu:236899.
    UCSCiuc009tsw.1. mouse. [Q811Q9-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY189949 mRNA. Translation: AAO39004.1 .
    AY189950 mRNA. Translation: AAO39005.1 .
    AK032027 mRNA. Translation: BAC27658.1 . Different initiation.
    AK053577 mRNA. Translation: BAC35435.1 .
    AK149304 mRNA. Translation: BAE28801.1 .
    BC048917 mRNA. Translation: AAH48917.1 .
    CCDSi CCDS30274.1. [Q811Q9-1 ]
    CCDS41061.1. [Q811Q9-2 ]
    RefSeqi NP_808214.1. NM_177546.2. [Q811Q9-2 ]
    NP_997593.1. NM_211138.1. [Q811Q9-1 ]
    UniGenei Mm.166467.

    3D structure databases

    ProteinModelPortali Q811Q9.
    SMRi Q811Q9. Positions 41-295.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 231814. 1 interaction.

    Proteomic databases

    PaxDbi Q811Q9.
    PRIDEi Q811Q9.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000045898 ; ENSMUSP00000044280 ; ENSMUSG00000035246 . [Q811Q9-1 ]
    ENSMUST00000113933 ; ENSMUSP00000109566 ; ENSMUSG00000035246 . [Q811Q9-2 ]
    GeneIDi 236899.
    KEGGi mmu:236899.
    UCSCi uc009tsw.1. mouse. [Q811Q9-1 ]

    Organism-specific databases

    CTDi 9468.
    MGIi MGI:2147987. Pcyt1b.

    Phylogenomic databases

    eggNOGi COG0615.
    GeneTreei ENSGT00390000000269.
    HOGENOMi HOG000230945.
    HOVERGENi HBG053531.
    InParanoidi Q811Q9.
    KOi K00968.
    OMAi LEHTCPH.
    OrthoDBi EOG7X9G6S.
    PhylomeDBi Q811Q9.
    TreeFami TF106336.

    Enzyme and pathway databases

    UniPathwayi UPA00753 ; UER00739 .
    Reactomei REACT_207335. Synthesis of PC.

    Miscellaneous databases

    NextBioi 383139.
    PROi Q811Q9.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q811Q9.
    Genevestigatori Q811Q9.

    Family and domain databases

    Gene3Di 3.40.50.620. 1 hit.
    InterProi IPR004821. Cyt_trans-like.
    IPR014729. Rossmann-like_a/b/a_fold.
    [Graphical view ]
    Pfami PF01467. CTP_transf_2. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00125. cyt_tran_rel. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Gene structure, expression and identification of a new CTP:phosphocholine cytidylyltransferase beta isoform."
      Karim M., Jackson P., Jackowski S.
      Biochim. Biophys. Acta 1633:1-12(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, FUNCTION.
      Strain: BALB/c.
      Tissue: Brain.
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 18-369 (ISOFORM 1).
      Strain: C57BL/6J.
      Tissue: Eye, Medulla oblongata and Retina.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Tissue: Olfactory epithelium.
    4. "Disruption of CCTbeta2 expression leads to gonadal dysfunction."
      Jackowski S., Rehg J.E., Zhang Y.-M., Wang J., Miller K., Jackson P., Karim M.A.
      Mol. Cell. Biol. 24:4720-4733(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.
    5. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-315 AND SER-319, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Embryonic brain.

    Entry informationi

    Entry nameiPCY1B_MOUSE
    AccessioniPrimary (citable) accession number: Q811Q9
    Secondary accession number(s): Q3UEW0
    , Q80Y63, Q811Q8, Q8BKD2, Q8C085
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 25, 2006
    Last sequence update: July 25, 2006
    Last modified: October 1, 2014
    This is version 86 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3