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Q811Q9

- PCY1B_MOUSE

UniProt

Q811Q9 - PCY1B_MOUSE

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Protein

Choline-phosphate cytidylyltransferase B

Gene

Pcyt1b

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Controls phosphatidylcholine synthesis.2 Publications

Catalytic activityi

CTP + phosphocholine = diphosphate + CDP-choline.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei122 – 1221CTPBy similarity
Binding sitei122 – 1221SubstrateBy similarity
Binding sitei151 – 1511SubstrateBy similarity
Binding sitei173 – 1731CTPBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi84 – 929CTPBy similarity
Nucleotide bindingi168 – 1692CTPBy similarity
Nucleotide bindingi196 – 2005CTPBy similarity

GO - Molecular functioni

  1. choline-phosphate cytidylyltransferase activity Source: UniProtKB-EC

GO - Biological processi

  1. ovarian follicle development Source: MGI
  2. spermatogenesis Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Biological processi

Lipid biosynthesis, Lipid metabolism, Phospholipid biosynthesis, Phospholipid metabolism

Enzyme and pathway databases

ReactomeiREACT_207335. Synthesis of PC.
UniPathwayiUPA00753; UER00739.

Names & Taxonomyi

Protein namesi
Recommended name:
Choline-phosphate cytidylyltransferase B (EC:2.7.7.15)
Alternative name(s):
CCT-beta
CTP:phosphocholine cytidylyltransferase B
Short name:
CCT B
Short name:
CT B
Phosphorylcholine transferase B
Gene namesi
Name:Pcyt1b
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome X

Organism-specific databases

MGIiMGI:2147987. Pcyt1b.

Subcellular locationi

Endoplasmic reticulum By similarity

GO - Cellular componenti

  1. endoplasmic reticulum Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum

Pathology & Biotechi

Disruption phenotypei

Female mice lacking isoform 1 have reduced fecundity due to failure in ovary maturation. Male mice lacking isoform 1 have reduced fecundity due to testicular degeneration.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 369369Choline-phosphate cytidylyltransferase BPRO_0000247762Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei315 – 3151Phosphoserine1 Publication
Modified residuei319 – 3191Phosphoserine1 Publication
Modified residuei362 – 3621PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ811Q9.
PaxDbiQ811Q9.
PRIDEiQ811Q9.

Expressioni

Tissue specificityi

Highly expressed in brain (at protein level). Expressed at lower levels in lung and gonads.2 Publications

Developmental stagei

Expression is low at E7, reaches a maximum at E15 and decreases at E17.1 Publication

Gene expression databases

BgeeiQ811Q9.
GenevestigatoriQ811Q9.

Interactioni

Protein-protein interaction databases

BioGridi231814. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliQ811Q9.
SMRiQ811Q9. Positions 41-295.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cytidylyltransferase family.Curated

Phylogenomic databases

eggNOGiCOG0615.
GeneTreeiENSGT00390000000269.
HOGENOMiHOG000230945.
HOVERGENiHBG053531.
InParanoidiQ811Q9.
KOiK00968.
OMAiLEHTCPH.
OrthoDBiEOG7X9G6S.
PhylomeDBiQ811Q9.
TreeFamiTF106336.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
InterProiIPR004821. Cyt_trans-like.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF01467. CTP_transf_2. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00125. cyt_tran_rel. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q811Q9-1) [UniParc]FASTAAdd to Basket

Also known as: CCTbeta2

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MPVLTTDAES ETGIPKSLSN EPPSETMEEI EHTCPQPRLT LTAPAPFADE
60 70 80 90 100
SSCQCQAPHE KLTVAQARLG TPVDRPVRVY ADGIFDLFHS GHARALMQAK
110 120 130 140 150
TLFPNSYLLV GVCSDDLTHK FKGFTVMNEA ERYEALRHCR YVDEVIRDAP
160 170 180 190 200
WTLTPEFLEK HKIDFVAHDD IPYSSAGSDD VYKHIKEAGM FVPTQRTEGI
210 220 230 240 250
STSDIITRIV RDYDVYARRN LQRGYTAKEL NVSFINEKKY RFQNQVDKMK
260 270 280 290 300
EKVKNVEERS KEFVNRVEEK SHDLIQKWEE KSREFIGNFL ELFGPDGAWK
310 320 330 340 350
QMFQERSSRM LQALSPKQSP VSSPTRSRSP SRSPSPTFSW LPNKTSPPSS
360
PKAASASISS MSEGDEDEK
Length:369
Mass (Da):41,900
Last modified:July 25, 2006 - v2
Checksum:i841B0E2E9EE22615
GO
Isoform 2 (identifier: Q811Q9-2) [UniParc]FASTAAdd to Basket

Also known as: CCTbeta3

The sequence of this isoform differs from the canonical sequence as follows:
     1-24: Missing.
     25-39: ETMEEIEHTCPQPRL → MDKDEFSRK

Note: Enzymatic activity identical to isoform 1. Not expressed in embryo. Expression starts at 5 weeks.

Show »
Length:339
Mass (Da):38,760
Checksum:i2B02AF5565877EBE
GO

Sequence cautioni

The sequence BAC27658.1 differs from that shown. Reason: Erroneous initiation.

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti73 – 731V → G in AAO39004. (PubMed:12842190)Curated
Sequence conflicti73 – 731V → G in AAO39005. (PubMed:12842190)Curated
Sequence conflicti77 – 771V → I in BAE28801. (PubMed:16141072)Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 2424Missing in isoform 2. 2 PublicationsVSP_020041Add
BLAST
Alternative sequencei25 – 3915ETMEE…PQPRL → MDKDEFSRK in isoform 2. 2 PublicationsVSP_020042Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY189949 mRNA. Translation: AAO39004.1.
AY189950 mRNA. Translation: AAO39005.1.
AK032027 mRNA. Translation: BAC27658.1. Different initiation.
AK053577 mRNA. Translation: BAC35435.1.
AK149304 mRNA. Translation: BAE28801.1.
BC048917 mRNA. Translation: AAH48917.1.
CCDSiCCDS30274.1. [Q811Q9-1]
CCDS41061.1. [Q811Q9-2]
RefSeqiNP_808214.1. NM_177546.2. [Q811Q9-2]
NP_997593.1. NM_211138.1. [Q811Q9-1]
UniGeneiMm.166467.

Genome annotation databases

EnsembliENSMUST00000045898; ENSMUSP00000044280; ENSMUSG00000035246. [Q811Q9-1]
ENSMUST00000113933; ENSMUSP00000109566; ENSMUSG00000035246. [Q811Q9-2]
GeneIDi236899.
KEGGimmu:236899.
UCSCiuc009tsw.1. mouse. [Q811Q9-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY189949 mRNA. Translation: AAO39004.1 .
AY189950 mRNA. Translation: AAO39005.1 .
AK032027 mRNA. Translation: BAC27658.1 . Different initiation.
AK053577 mRNA. Translation: BAC35435.1 .
AK149304 mRNA. Translation: BAE28801.1 .
BC048917 mRNA. Translation: AAH48917.1 .
CCDSi CCDS30274.1. [Q811Q9-1 ]
CCDS41061.1. [Q811Q9-2 ]
RefSeqi NP_808214.1. NM_177546.2. [Q811Q9-2 ]
NP_997593.1. NM_211138.1. [Q811Q9-1 ]
UniGenei Mm.166467.

3D structure databases

ProteinModelPortali Q811Q9.
SMRi Q811Q9. Positions 41-295.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 231814. 1 interaction.

Proteomic databases

MaxQBi Q811Q9.
PaxDbi Q811Q9.
PRIDEi Q811Q9.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000045898 ; ENSMUSP00000044280 ; ENSMUSG00000035246 . [Q811Q9-1 ]
ENSMUST00000113933 ; ENSMUSP00000109566 ; ENSMUSG00000035246 . [Q811Q9-2 ]
GeneIDi 236899.
KEGGi mmu:236899.
UCSCi uc009tsw.1. mouse. [Q811Q9-1 ]

Organism-specific databases

CTDi 9468.
MGIi MGI:2147987. Pcyt1b.

Phylogenomic databases

eggNOGi COG0615.
GeneTreei ENSGT00390000000269.
HOGENOMi HOG000230945.
HOVERGENi HBG053531.
InParanoidi Q811Q9.
KOi K00968.
OMAi LEHTCPH.
OrthoDBi EOG7X9G6S.
PhylomeDBi Q811Q9.
TreeFami TF106336.

Enzyme and pathway databases

UniPathwayi UPA00753 ; UER00739 .
Reactomei REACT_207335. Synthesis of PC.

Miscellaneous databases

NextBioi 383139.
PROi Q811Q9.
SOURCEi Search...

Gene expression databases

Bgeei Q811Q9.
Genevestigatori Q811Q9.

Family and domain databases

Gene3Di 3.40.50.620. 1 hit.
InterProi IPR004821. Cyt_trans-like.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view ]
Pfami PF01467. CTP_transf_2. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00125. cyt_tran_rel. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Gene structure, expression and identification of a new CTP:phosphocholine cytidylyltransferase beta isoform."
    Karim M., Jackson P., Jackowski S.
    Biochim. Biophys. Acta 1633:1-12(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, FUNCTION.
    Strain: BALB/c.
    Tissue: Brain.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 18-369 (ISOFORM 1).
    Strain: C57BL/6J.
    Tissue: Eye, Medulla oblongata and Retina.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Olfactory epithelium.
  4. "Disruption of CCTbeta2 expression leads to gonadal dysfunction."
    Jackowski S., Rehg J.E., Zhang Y.-M., Wang J., Miller K., Jackson P., Karim M.A.
    Mol. Cell. Biol. 24:4720-4733(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.
  5. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-315 AND SER-319, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic brain.

Entry informationi

Entry nameiPCY1B_MOUSE
AccessioniPrimary (citable) accession number: Q811Q9
Secondary accession number(s): Q3UEW0
, Q80Y63, Q811Q8, Q8BKD2, Q8C085
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 25, 2006
Last sequence update: July 25, 2006
Last modified: October 29, 2014
This is version 87 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3