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Protein

Choline-phosphate cytidylyltransferase B

Gene

Pcyt1b

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Controls phosphatidylcholine synthesis.2 Publications

Catalytic activityi

CTP + phosphocholine = diphosphate + CDP-choline.By similarity

Pathwayi: phosphatidylcholine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes phosphatidylcholine from phosphocholine.By similarity
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Choline-phosphate cytidylyltransferase A (Pcyt1a), Choline-phosphate cytidylyltransferase B (Pcyt1b)
  2. Cholinephosphotransferase 1 (Chpt1), Choline/ethanolaminephosphotransferase 1 (Cept1)
This subpathway is part of the pathway phosphatidylcholine biosynthesis, which is itself part of Phospholipid metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes phosphatidylcholine from phosphocholine, the pathway phosphatidylcholine biosynthesis and in Phospholipid metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei122CTPBy similarity1
Binding sitei122SubstrateBy similarity1
Binding sitei151SubstrateBy similarity1
Binding sitei173CTPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi84 – 92CTPBy similarity9
Nucleotide bindingi168 – 169CTPBy similarity2
Nucleotide bindingi196 – 200CTPBy similarity5

GO - Molecular functioni

GO - Biological processi

  • ovarian follicle development Source: MGI
  • spermatogenesis Source: MGI

Keywordsi

Molecular functionNucleotidyltransferase, Transferase
Biological processLipid biosynthesis, Lipid metabolism, Phospholipid biosynthesis, Phospholipid metabolism

Enzyme and pathway databases

BRENDAi2.7.7.15 3474
ReactomeiR-MMU-1483191 Synthesis of PC
UniPathwayiUPA00753; UER00739

Names & Taxonomyi

Protein namesi
Recommended name:
Choline-phosphate cytidylyltransferase B (EC:2.7.7.15By similarity)
Alternative name(s):
CCT-beta
CTP:phosphocholine cytidylyltransferase B
Short name:
CCT B
Short name:
CT B
Phosphorylcholine transferase B
Gene namesi
Name:Pcyt1b
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome X

Organism-specific databases

MGIiMGI:2147987 Pcyt1b

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Endoplasmic reticulum

Pathology & Biotechi

Disruption phenotypei

Female mice lacking isoform 1 have reduced fecundity due to failure in ovary maturation. Male mice lacking isoform 1 have reduced fecundity due to testicular degeneration.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002477621 – 369Choline-phosphate cytidylyltransferase BAdd BLAST369

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei315PhosphoserineCombined sources1
Modified residuei319PhosphoserineCombined sources1
Modified residuei322PhosphoserineBy similarity1
Modified residuei323PhosphoserineCombined sources1
Modified residuei329PhosphoserineBy similarity1
Modified residuei331PhosphoserineBy similarity1
Modified residuei335PhosphoserineBy similarity1
Modified residuei345PhosphothreonineBy similarity1
Modified residuei346PhosphoserineBy similarity1
Modified residuei349PhosphoserineBy similarity1
Modified residuei350PhosphoserineBy similarity1
Modified residuei355PhosphoserineBy similarity1
Modified residuei360PhosphoserineCombined sources1
Modified residuei362PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ811Q9
PeptideAtlasiQ811Q9
PRIDEiQ811Q9

PTM databases

iPTMnetiQ811Q9
PhosphoSitePlusiQ811Q9

Expressioni

Tissue specificityi

Highly expressed in brain (at protein level). Expressed at lower levels in lung and gonads.2 Publications

Developmental stagei

Expression is low at E7, reaches a maximum at E15 and decreases at E17.1 Publication

Gene expression databases

BgeeiENSMUSG00000035246
GenevisibleiQ811Q9 MM

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000044280

Structurei

3D structure databases

ProteinModelPortaliQ811Q9
SMRiQ811Q9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cytidylyltransferase family.Curated

Phylogenomic databases

eggNOGiKOG2804 Eukaryota
COG0615 LUCA
GeneTreeiENSGT00390000000269
HOGENOMiHOG000230945
HOVERGENiHBG053531
InParanoidiQ811Q9
KOiK00968
OMAiLEHTCPH
OrthoDBiEOG091G0DDW
PhylomeDBiQ811Q9
TreeFamiTF106336

Family and domain databases

Gene3Di3.40.50.620, 1 hit
InterProiView protein in InterPro
IPR004821 Cyt_trans-like
IPR014729 Rossmann-like_a/b/a_fold
PfamiView protein in Pfam
PF01467 CTP_transf_like, 1 hit
TIGRFAMsiTIGR00125 cyt_tran_rel, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q811Q9-1) [UniParc]FASTAAdd to basket
Also known as: CCTbeta2

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MPVLTTDAES ETGIPKSLSN EPPSETMEEI EHTCPQPRLT LTAPAPFADE
60 70 80 90 100
SSCQCQAPHE KLTVAQARLG TPVDRPVRVY ADGIFDLFHS GHARALMQAK
110 120 130 140 150
TLFPNSYLLV GVCSDDLTHK FKGFTVMNEA ERYEALRHCR YVDEVIRDAP
160 170 180 190 200
WTLTPEFLEK HKIDFVAHDD IPYSSAGSDD VYKHIKEAGM FVPTQRTEGI
210 220 230 240 250
STSDIITRIV RDYDVYARRN LQRGYTAKEL NVSFINEKKY RFQNQVDKMK
260 270 280 290 300
EKVKNVEERS KEFVNRVEEK SHDLIQKWEE KSREFIGNFL ELFGPDGAWK
310 320 330 340 350
QMFQERSSRM LQALSPKQSP VSSPTRSRSP SRSPSPTFSW LPNKTSPPSS
360
PKAASASISS MSEGDEDEK
Length:369
Mass (Da):41,900
Last modified:July 25, 2006 - v2
Checksum:i841B0E2E9EE22615
GO
Isoform 2 (identifier: Q811Q9-2) [UniParc]FASTAAdd to basket
Also known as: CCTbeta3

The sequence of this isoform differs from the canonical sequence as follows:
     1-24: Missing.
     25-39: ETMEEIEHTCPQPRL → MDKDEFSRK

Note: Enzymatic activity identical to isoform 1. Not expressed in embryo. Expression starts at 5 weeks.
Show »
Length:339
Mass (Da):38,760
Checksum:i2B02AF5565877EBE
GO

Sequence cautioni

The sequence BAC27658 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti73V → G in AAO39004 (PubMed:12842190).Curated1
Sequence conflicti73V → G in AAO39005 (PubMed:12842190).Curated1
Sequence conflicti77V → I in BAE28801 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0200411 – 24Missing in isoform 2. 2 PublicationsAdd BLAST24
Alternative sequenceiVSP_02004225 – 39ETMEE…PQPRL → MDKDEFSRK in isoform 2. 2 PublicationsAdd BLAST15

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY189949 mRNA Translation: AAO39004.1
AY189950 mRNA Translation: AAO39005.1
AK032027 mRNA Translation: BAC27658.1 Different initiation.
AK053577 mRNA Translation: BAC35435.1
AK149304 mRNA Translation: BAE28801.1
BC048917 mRNA Translation: AAH48917.1
CCDSiCCDS30274.1 [Q811Q9-1]
CCDS41061.1 [Q811Q9-2]
RefSeqiNP_808214.1, NM_177546.2 [Q811Q9-2]
NP_997593.1, NM_211138.1 [Q811Q9-1]
UniGeneiMm.166467

Genome annotation databases

EnsembliENSMUST00000045898; ENSMUSP00000044280; ENSMUSG00000035246 [Q811Q9-1]
ENSMUST00000113933; ENSMUSP00000109566; ENSMUSG00000035246 [Q811Q9-2]
GeneIDi236899
KEGGimmu:236899
UCSCiuc009tsv.1 mouse [Q811Q9-2]
uc009tsw.1 mouse [Q811Q9-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiPCY1B_MOUSE
AccessioniPrimary (citable) accession number: Q811Q9
Secondary accession number(s): Q3UEW0
, Q80Y63, Q811Q8, Q8BKD2, Q8C085
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 25, 2006
Last sequence update: July 25, 2006
Last modified: March 28, 2018
This is version 109 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families
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Main funding by: National Institutes of Health