Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Chloride intracellular channel protein 6

Gene

Clic6

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May insert into membranes and form chloride ion channels. May play a critical role in water-secreting cells, possibly through the regulation of chloride ion transport (By similarity).By similarity

GO - Molecular functioni

  • chloride channel activity Source: GO_Central
  • D2 dopamine receptor binding Source: RGD
  • D3 dopamine receptor binding Source: RGD
  • D4 dopamine receptor binding Source: RGD
  • protein C-terminus binding Source: RGD
  • protein homodimerization activity Source: RGD
  • voltage-gated ion channel activity Source: UniProtKB-KW

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Chloride channel, Ion channel, Voltage-gated channel

Keywords - Biological processi

Ion transport, Transport

Keywords - Ligandi

Chloride

Names & Taxonomyi

Protein namesi
Recommended name:
Chloride intracellular channel protein 6
Gene namesi
Name:Clic6
Synonyms:Clic6b
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi727938. Clic6.

Subcellular locationi

  • Cytoplasm By similarity
  • Cell membrane 1 Publication; Single-pass membrane protein 1 Publication

  • Note: Predominantly cytoplasmic. Upon chloride ion efflux from the cell, it is translocated to the plasma membrane (By similarity). Colocalizes with DRD3 at the plasma membrane.By similarity

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei397 – 41721Helical; Note=After insertion into the membraneSequence analysisAdd
BLAST

GO - Cellular componenti

  • chloride channel complex Source: UniProtKB-KW
  • cytoplasm Source: GO_Central
  • plasma membrane Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 612612Chloride intracellular channel protein 6PRO_0000144220Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei40 – 401PhosphoserineCombined sources
Modified residuei264 – 2641PhosphoserineCombined sources
Modified residuei303 – 3031PhosphoserineCombined sources
Modified residuei321 – 3211PhosphoserineCombined sources
Modified residuei368 – 3681PhosphoserineCombined sources

Post-translational modificationi

Phosphorylated.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ811Q2.
PRIDEiQ811Q2.

PTM databases

iPTMnetiQ811Q2.

Expressioni

Tissue specificityi

Predominanlty expressed in brain, pituitary and stomach. In adult brain, it is restricted to the choroid plexus, the striatal proliferative subventricular zone and the cerebellum where it colocalizes with the D3R in the Purkinje cells of the lobules IX and X.1 Publication

Interactioni

Subunit structurei

Interacts with dopamine receptors DRD2, DRD3 and DRD4.1 Publication

GO - Molecular functioni

  • D2 dopamine receptor binding Source: RGD
  • D3 dopamine receptor binding Source: RGD
  • D4 dopamine receptor binding Source: RGD
  • protein C-terminus binding Source: RGD
  • protein homodimerization activity Source: RGD

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000033206.

Structurei

3D structure databases

ProteinModelPortaliQ811Q2.
SMRiQ811Q2. Positions 375-611.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini464 – 612149GST C-terminalAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi134 – 370237Glu-richAdd
BLAST

Domaini

Members of this family may change from a globular, soluble state to a state where the N-terminal domain is inserted into the membrane and functions as chloride channel. A conformation change of the N-terminal domain is thought to expose hydrophobic surfaces that trigger membrane insertion (By similarity).By similarity

Sequence similaritiesi

Belongs to the chloride channel CLIC family.Curated
Contains 1 GST C-terminal domain.Curated

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1422. Eukaryota.
ENOG410ZRK6. LUCA.
HOGENOMiHOG000013008.
HOVERGENiHBG050996.
InParanoidiQ811Q2.
KOiK05026.
PhylomeDBiQ811Q2.

Family and domain databases

Gene3Di1.20.1050.10. 1 hit.
3.40.30.10. 1 hit.
InterProiIPR002946. CLIC.
IPR030263. CLIC-6.
IPR010987. Glutathione-S-Trfase_C-like.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PANTHERiPTHR11260:SF142. PTHR11260:SF142. 2 hits.
PRINTSiPR01263. INTCLCHANNEL.
SUPFAMiSSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 1 hit.
TIGRFAMsiTIGR00862. O-ClC. 1 hit.
PROSITEiPS50405. GST_CTER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q811Q2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAEATEPKEV SSGSQGQPEG AVIEGPGEPG AADLEGREAS EGAAEAPRDL
60 70 80 90 100
GEGAEAMASG KEEGGCGQDG EIGEVQAQDP RPGPGTETPG TSGAPGEAEA
110 120 130 140 150
AECDSEGALI PQSGGGAKRQ QVQGTSSGLD AQGEAPEVPE DARREPEDPK
160 170 180 190 200
ASEAGEEAES GQEALGGSAP ESQINPEVQG PVGDNMDTEA PAGEPQGSEG
210 220 230 240 250
EPQGGGESSP QPQDEAIEIA AAEVGGHEPG ELAGASAADA KGEGETLRKD
260 270 280 290 300
GFEEAAPEEA RVDSGENGFE EAAPEEARVD SGENRDQGRL QEETGEEEAR
310 320 330 340 350
PESGLKGPCE EAIQEKAPDG SLDGEEAKST GHEESQVELS NHLAEETSAQ
360 370 380 390 400
GGEELGRVNG RRENGPASEE GDLGQEHDIT LFVKAGSDGE SIGNCPFSQR
410 420 430 440 450
LFMILWLKGV IFNVTTVDLK RKPADLQNLA PGTNPPFMTF DGEVKTDVNK
460 470 480 490 500
IEEFLEEKLV PPRYPKLGTQ HPESNSAGND VFAKFSAFIK NTKKDANDIY
510 520 530 540 550
EKNLLRALKK LDSYLNSPLP DEIDAYSTED VTVSQRKFLD GDELTLADCN
560 570 580 590 600
LLPKLHIIKI VAKKYRGFEF PSEMTGIWRY LNNAYARDEF TNTCPADQEI
610
EHAYSDAAKR MK
Length:612
Mass (Da):64,687
Last modified:June 1, 2003 - v1
Checksum:iE9868AB4BDEF58D0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY191249 mRNA. Translation: AAO38057.1.
RefSeqiNP_788267.1. NM_176078.2.
UniGeneiRn.214050.

Genome annotation databases

GeneIDi304081.
KEGGirno:304081.
UCSCiRGD:727938. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY191249 mRNA. Translation: AAO38057.1.
RefSeqiNP_788267.1. NM_176078.2.
UniGeneiRn.214050.

3D structure databases

ProteinModelPortaliQ811Q2.
SMRiQ811Q2. Positions 375-611.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000033206.

PTM databases

iPTMnetiQ811Q2.

Proteomic databases

PaxDbiQ811Q2.
PRIDEiQ811Q2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi304081.
KEGGirno:304081.
UCSCiRGD:727938. rat.

Organism-specific databases

CTDi54102.
RGDi727938. Clic6.

Phylogenomic databases

eggNOGiKOG1422. Eukaryota.
ENOG410ZRK6. LUCA.
HOGENOMiHOG000013008.
HOVERGENiHBG050996.
InParanoidiQ811Q2.
KOiK05026.
PhylomeDBiQ811Q2.

Miscellaneous databases

PROiQ811Q2.

Family and domain databases

Gene3Di1.20.1050.10. 1 hit.
3.40.30.10. 1 hit.
InterProiIPR002946. CLIC.
IPR030263. CLIC-6.
IPR010987. Glutathione-S-Trfase_C-like.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PANTHERiPTHR11260:SF142. PTHR11260:SF142. 2 hits.
PRINTSiPR01263. INTCLCHANNEL.
SUPFAMiSSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 1 hit.
TIGRFAMsiTIGR00862. O-ClC. 1 hit.
PROSITEiPS50405. GST_CTER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "CLIC6, a member of the intracellular chloride channel family, interacts with dopamine D(2)-like receptors."
    Griffon N., Jeanneteau F., Prieur F., Diaz J., Sokoloff P.
    Brain Res. Mol. Brain Res. 117:47-57(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INTERACTION WITH DRD2; DRD3 AND DRD4.
    Strain: Sprague-Dawley.
    Tissue: Brain.
  2. "Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues."
    Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C., Olsen J.V.
    Nat. Commun. 3:876-876(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-40; SER-264; SER-303; SER-321 AND SER-368, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiCLIC6_RAT
AccessioniPrimary (citable) accession number: Q811Q2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 2005
Last sequence update: June 1, 2003
Last modified: July 6, 2016
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.