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Protein

Single-strand selective monofunctional uracil-DNA glycosylase

Gene

Smug1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Recognizes base lesions in the genome and initiates base excision DNA repair. Acts as a monofunctional DNA glycosylase specific for uracil (U) residues in DNA with a preference for single-stranded DNA substrates. The activity is greater toward mismatches (U/G) compared to matches (U/A). Excises uracil (U), 5-formyluracil (fU) and uracil derivatives bearing an oxidized group at C5 [5-hydroxyuracil (hoU) and 5-hydroxymethyluracil (hmU)] in ssDNA and dsDNA, but not analogous cytosine derivatives (5-hydroxycytosine and 5-formylcytosine), nor other oxidized bases. The activity is damage-specific and salt-dependent. The substrate preference is the following: ssDNA > dsDNA (G pair) = dsDNA (A pair) at low salt concentration, and dsDNA (G pair) > dsDNA (A pair) > ssDNA at high salt concentration.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei84Substrate; via amide nitrogenBy similarity1
Binding sitei98Substrate; via amide nitrogenBy similarity1
Binding sitei163SubstrateBy similarity1
Binding sitei239SubstrateBy similarity1

GO - Molecular functioni

  • DNA binding Source: UniProtKB-KW
  • oxidized pyrimidine nucleobase lesion DNA N-glycosylase activity Source: RGD
  • single-strand selective uracil DNA N-glycosylase activity Source: HGNC
  • uracil DNA N-glycosylase activity Source: HGNC

GO - Biological processi

  • base-excision repair Source: HGNC
  • DNA repair Source: RGD

Keywordsi

Molecular functionDNA-binding, Hydrolase
Biological processDNA damage, DNA repair

Enzyme and pathway databases

BRENDAi3.2.2.27 5301
ReactomeiR-RNO-110329 Cleavage of the damaged pyrimidine
R-RNO-110357 Displacement of DNA glycosylase by APEX1

Names & Taxonomyi

Protein namesi
Recommended name:
Single-strand selective monofunctional uracil-DNA glycosylase (EC:3.2.2.-)
Gene namesi
Name:Smug1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 7

Organism-specific databases

RGDi631403 Smug1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000719941 – 278Single-strand selective monofunctional uracil-DNA glycosylaseAdd BLAST278

Proteomic databases

PaxDbiQ811Q1
PRIDEiQ811Q1

Expressioni

Gene expression databases

BgeeiENSRNOG00000036842

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000052164

Structurei

3D structure databases

ProteinModelPortaliQ811Q1
SMRiQ811Q1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni173 – 187DNA bindingBy similarityAdd BLAST15

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG410IFBA Eukaryota
ENOG410XQUR LUCA
GeneTreeiENSGT00390000004897
HOGENOMiHOG000220288
HOVERGENiHBG084399
InParanoidiQ811Q1
KOiK10800
OMAiRFWGFFR
OrthoDBiEOG091G0R85
PhylomeDBiQ811Q1
TreeFamiTF324356

Family and domain databases

Gene3Di3.40.470.10, 1 hit
InterProiView protein in InterPro
IPR036895 Uracil-DNA_glycosylase-like_sf
SUPFAMiSSF52141 SSF52141, 1 hit

Sequencei

Sequence statusi: Complete.

Q811Q1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAVSQTFPPG PAHEPASALM EPCARSLAEG FLEEELRLNA ELSQLQFPEP
60 70 80 90 100
VGVIYNPVDY AWEPHRNYVT RYCQGPKEVL FLGMNPGPFG MAQTGVPFGE
110 120 130 140 150
VNVVRDWLGI GGSVLSPPQE HPKRPVLGLE CPQSEVSGAR FWGFFRTLCG
160 170 180 190 200
QPQVFFRHCF VHNLCPLLFL APSGRNLTPA DLPAKHREQL LSICDAALCR
210 220 230 240 250
QVQLLGVRLV VGVGRLAEQR ARRALAGLTP EVQVEGLLHP SPRSPQANKG
260 270
WETAARERLQ ELGLLPLLTD EGSVRPTP
Length:278
Mass (Da):30,562
Last modified:June 1, 2003 - v1
Checksum:iC1A047F60AB76F56
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY191521 mRNA Translation: AAO38671.1
RefSeqiNP_808795.1, NM_177934.3
XP_008763971.1, XM_008765749.2
XP_017450389.1, XM_017594900.1
XP_017450390.1, XM_017594901.1
XP_017450391.1, XM_017594902.1
UniGeneiRn.162974

Genome annotation databases

EnsembliENSRNOT00000055290; ENSRNOP00000052164; ENSRNOG00000036842
ENSRNOT00000082149; ENSRNOP00000069685; ENSRNOG00000036842
GeneIDi315344
KEGGirno:315344

Similar proteinsi

Entry informationi

Entry nameiSMUG1_RAT
AccessioniPrimary (citable) accession number: Q811Q1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 16, 2005
Last sequence update: June 1, 2003
Last modified: May 23, 2018
This is version 100 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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