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Q811Q0 (KCMB2_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 70. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Calcium-activated potassium channel subunit beta-2
Alternative name(s):
BK channel subunit beta-2
Short name=BKbeta2
Calcium-activated potassium channel, subfamily M subunit beta-2
Charybdotoxin receptor subunit beta-2
K(VCA)beta-2
Maxi K channel subunit beta-2
Rbeta3
Slo-beta-2
Gene names
Name:Kcnmb2
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length235 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Regulatory subunit of the calcium activated potassium KCNMA1 (maxiK) channel. Modulates the calcium sensitivity and gating kinetics of KCNMA1, thereby contributing to KCNMA1 channel diversity. Acts as a negative regulator that confers rapid and complete inactivation of KCNMA1 channel complex By similarity.

Subunit structure

Interacts with KCNMA1 tetramer. There are probably 4 molecules of KCMNB2 per KCNMA1 tetramer By similarity.

Subcellular location

Membrane; Multi-pass membrane protein By similarity.

Tissue specificity

Highly expressed in brain and heart. Also expressed in lung. Ref.3

Domain

The ball and chain domain mediates the inactivation of KCNMA1. It occludes the conduction pathway of KCNMA1 channels, and comprises the pore-blocking ball domain (residues 1-17) and the chain domain (residues 20-45) linking it to the transmembrane segment. The ball domain is made up of a flexible N-terminus anchored at a well ordered loop-helix motif. The chain domain consists of a 4-turn helix with an unfolded linker at its C-terminus By similarity.

Post-translational modification

N-glycosylated By similarity.

Sequence similarities

Belongs to the KCNMB (TC 8.A.14.1) family. KCNMB2 subfamily. [View classification]

Ontologies

Keywords
   Biological processIon transport
Transport
   Cellular componentMembrane
   DomainTransmembrane
Transmembrane helix
   Molecular functionIon channel
   PTMGlycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processpotassium ion transmembrane transport

Inferred from direct assay Ref.2. Source: GOC

   Cellular_componentintegral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functioncalcium-activated potassium channel activity

Inferred from electronic annotation. Source: InterPro

potassium channel activity

Inferred from direct assay Ref.2. Source: RGD

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 235235Calcium-activated potassium channel subunit beta-2
PRO_0000187053

Regions

Topological domain1 – 4646Cytoplasmic Potential
Transmembrane47 – 6721Helical; Name=1; Potential
Topological domain68 – 194127Extracellular Potential
Transmembrane195 – 21521Helical; Name=2; Potential
Topological domain216 – 23520Cytoplasmic Potential
Region1 – 4545Ball and chain

Amino acid modifications

Glycosylation881N-linked (GlcNAc...) Potential
Glycosylation961N-linked (GlcNAc...) Potential
Glycosylation1191N-linked (GlcNAc...) Potential

Experimental info

Sequence conflict841R → C in CAD56888. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Q811Q0 [UniParc].

Last modified June 1, 2003. Version 1.
Checksum: E111B041867A3F70

FASTA23527,154
        10         20         30         40         50         60 
MFIWTSGRTS SSYRHDEKRN IYQKIRDHDL LDKRKTVTAL KAGEDRAILL GLAMMVCSIM 

        70         80         90        100        110        120 
MYFLLGITLL RSYMQSVWTE EAQRALLNVS ITETFNCSFS CGPDCWKLSQ YPCLQVYVNL 

       130        140        150        160        170        180 
TSSGEKLLLY HTEETMKINQ KCSYIPKCGN NFEESMSLVS VVMENFRRHQ HFPCYSDPEG 

       190        200        210        220        230 
NQKSVILTKL YSSNVLFHSL FWPTCMMAGG VAIVAMVKLT QYLSLLCERI QRINR 

« Hide

References

[1]"Rat inactivating beta 2 subunit of large conductance Ca2+-activated K+ channel (KCNMB2, rSlo beta 2 subunit)."
Eghbali M., Foroughi S., Toro L., Stefani E.
Submitted (DEC-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: Sprague-Dawley.
Tissue: Heart.
[2]"Expression of a Ca2+-activated BK channel mRNA and its splice variants in the rat cochlea."
Langer P., Gruender S., Ruesch A.
J. Comp. Neurol. 455:198-209(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 40-201.
Strain: Wistar.
Tissue: Brain.
[3]"Molecular basis for the inactivation of Ca2+- and voltage-dependent BK channels in adrenal chromaffin cells and rat insulinoma tumor cells."
Xia X.-M., Ding J.-P., Lingle C.J.
J. Neurosci. 19:5255-5264(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY191836 mRNA. Translation: AAO43501.1.
AJ517198 mRNA. Translation: CAD56888.1.
RefSeqNP_789831.1. NM_176861.1.
UniGeneRn.220612.
Rn.92476.

3D structure databases

ProteinModelPortalQ811Q0.
SMRQ811Q0. Positions 1-45.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PRIDEQ811Q0.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID294961.
KEGGrno:294961.
UCSCRGD:631398. rat.

Organism-specific databases

CTD10242.
RGD631398. Kcnmb2.

Phylogenomic databases

eggNOGNOG44064.
HOGENOMHOG000290180.
HOVERGENHBG052223.
InParanoidQ811Q0.
KOK04938.
PhylomeDBQ811Q0.

Gene expression databases

GenevestigatorQ811Q0.

Family and domain databases

Gene3D4.10.81.20. 1 hit.
InterProIPR003930. K_chnl_Ca-activ_BK_bsu.
IPR015382. KCNMB2_ball_chain_dom.
[Graphical view]
PANTHERPTHR10258. PTHR10258. 1 hit.
PfamPF03185. CaKB. 1 hit.
PF09303. KcnmB2_inactiv. 1 hit.
[Graphical view]
PRINTSPR01450. BKCHANNELB.
ProtoNetSearch...

Other

NextBio638780.
PROQ811Q0.

Entry information

Entry nameKCMB2_RAT
AccessionPrimary (citable) accession number: Q811Q0
Secondary accession number(s): Q8CF83
Entry history
Integrated into UniProtKB/Swiss-Prot: April 13, 2004
Last sequence update: June 1, 2003
Last modified: April 16, 2014
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families