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Protein

Rho GTPase-activating protein 15

Gene

Arhgap15

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

GTPase activator for the Rho-type GTPases by converting them to an inactive GDP-bound state. Has activity toward RAC1. Overexpression results in an increase in actin stress fibers and cell contraction (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

GTPase activation

Enzyme and pathway databases

ReactomeiR-MMU-194840. Rho GTPase cycle.

Names & Taxonomyi

Protein namesi
Recommended name:
Rho GTPase-activating protein 15
Alternative name(s):
ArhGAP15
Rho-type GTPase-activating protein 15
Gene namesi
Name:Arhgap15
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:1923367. Arhgap15.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 481481Rho GTPase-activating protein 15PRO_0000317575Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei51 – 511PhosphoserineBy similarity
Modified residuei111 – 1111PhosphoserineBy similarity
Modified residuei204 – 2041PhosphoserineCombined sources
Modified residuei207 – 2071PhosphoserineBy similarity
Modified residuei249 – 2491PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ811M1.
MaxQBiQ811M1.
PaxDbiQ811M1.
PRIDEiQ811M1.

PTM databases

iPTMnetiQ811M1.
PhosphoSiteiQ811M1.

Expressioni

Gene expression databases

BgeeiQ811M1.
CleanExiMM_ARHGAP15.
ExpressionAtlasiQ811M1. baseline and differential.
GenevisibleiQ811M1. MM.

Interactioni

Protein-protein interaction databases

BioGridi217972. 2 interactions.
STRINGi10090.ENSMUSP00000056461.

Structurei

3D structure databases

ProteinModelPortaliQ811M1.
SMRiQ811M1. Positions 88-199, 268-476.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini87 – 197111PHPROSITE-ProRule annotationAdd
BLAST
Domaini287 – 476190Rho-GAPPROSITE-ProRule annotationAdd
BLAST

Domaini

The PH domain is required for localization to the membrane.By similarity

Sequence similaritiesi

Contains 1 PH domain.PROSITE-ProRule annotation
Contains 1 Rho-GAP domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG1449. Eukaryota.
KOG1450. Eukaryota.
ENOG410ZP6T. LUCA.
GeneTreeiENSGT00840000129684.
HOGENOMiHOG000294167.
HOVERGENiHBG005328.
InParanoidiQ811M1.
OMAiQDNATRI.
PhylomeDBiQ811M1.
TreeFamiTF329345.

Family and domain databases

Gene3Di1.10.555.10. 1 hit.
2.30.29.30. 1 hit.
InterProiIPR011993. PH_dom-like.
IPR001849. PH_domain.
IPR015767. Rho_GTPase_act.
IPR008936. Rho_GTPase_activation_prot.
IPR000198. RhoGAP_dom.
[Graphical view]
PANTHERiPTHR23181. PTHR23181. 1 hit.
PfamiPF00169. PH. 1 hit.
PF00620. RhoGAP. 1 hit.
[Graphical view]
SMARTiSM00233. PH. 1 hit.
SM00324. RhoGAP. 1 hit.
[Graphical view]
SUPFAMiSSF48350. SSF48350. 1 hit.
SSF50729. SSF50729. 1 hit.
PROSITEiPS50003. PH_DOMAIN. 1 hit.
PS50238. RHOGAP. 1 hit.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q811M1-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEKRTSCSVQ TSTNCDNSLE ILNSAHQATG AVQMRIKNAN SHQDRQSQTK
60 70 80 90 100
SMILTDAGKV TEPISRHRRN HSQHVLKDVI PPLEHPMVEK EGYLQKAKIA
110 120 130 140 150
DGGKKLRKNW STSWIVLSGR KIEFYKDSKQ QALPNMKTRH NVESVDLCGA
160 170 180 190 200
HIEWAKEKSS RKSVFQITTV SGNEFLLQSD IDFLILDWFQ AIKNAIDRLP
210 220 230 240 250
KNPSCGSLEL FNLQRSSSSE LPSHCHIDRK EQKPEHRKSF MFRLHHSASD
260 270 280 290 300
TSDKNRVKSR LKKFISRRPS LKTLQEKGLI KDQIFGSHLH TVCEREHSTV
310 320 330 340 350
PWFVKQCIEA VEKRGLDVDG IYRVSGNLAT IQKLRFIVNQ EEKLNLDDSQ
360 370 380 390 400
WEDIHVVTGA LKMFFRELSE PLFPYSFFER FVEAIKKQDS NEKIETMRSL
410 420 430 440 450
VKRLPPPNHD TMKILFRHLT KIVAKASQNL MSTQSLGIVF GPTLLRAENE
460 470 480
SGNVAVHMVY QNQIAEFMLT EYDKIFSSEE D
Length:481
Mass (Da):55,339
Last modified:June 1, 2003 - v1
Checksum:iB679FC5159BE20B2
GO
Isoform 2 (identifier: Q811M1-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     316-316: L → G
     317-481: Missing.

Note: No experimental confirmation available.
Show »
Length:316
Mass (Da):36,213
Checksum:iE01897D2B49F0675
GO
Isoform 3 (identifier: Q811M1-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     167-186: ITTVSGNEFLLQSDIDFLIL → VRMPPVLNFLLFPPNLSPLR
     187-481: Missing.

Note: No experimental confirmation available.
Show »
Length:186
Mass (Da):21,179
Checksum:iD6B39E2A92CBE4FA
GO
Isoform 4 (identifier: Q811M1-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     109-481: Missing.

Note: No experimental confirmation available.
Show »
Length:108
Mass (Da):12,114
Checksum:iCF58919A4D40B797
GO

Sequence cautioni

The sequence AAH34881.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti46 – 461Q → K in BAE41865 (PubMed:16141072).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei109 – 481373Missing in isoform 4. 1 PublicationVSP_031048Add
BLAST
Alternative sequencei167 – 18620ITTVS…DFLIL → VRMPPVLNFLLFPPNLSPLR in isoform 3. 1 PublicationVSP_031049Add
BLAST
Alternative sequencei187 – 481295Missing in isoform 3. 1 PublicationVSP_031050Add
BLAST
Alternative sequencei316 – 3161L → G in isoform 2. 1 PublicationVSP_031051
Alternative sequencei317 – 481165Missing in isoform 2. 1 PublicationVSP_031052Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK031022 mRNA. Translation: BAC27217.1.
AK170537 mRNA. Translation: BAE41865.1.
AK172407 mRNA. Translation: BAE42992.1.
AL732320
, AL844535, AL845160, AL928611, AL935152 Genomic DNA. Translation: CAM15030.1.
AL844535
, AL732320, AL845160, AL928611, AL935152 Genomic DNA. Translation: CAM17611.1.
AL928611
, AL732320, AL844535, AL845160, AL935152 Genomic DNA. Translation: CAM19036.1.
AL845160
, AL732320, AL844535, AL928611, AL935152 Genomic DNA. Translation: CAM20252.1.
AL935152
, AL732320, AL844535, AL845160, AL928611 Genomic DNA. Translation: CAM27855.1.
BC024887 mRNA. Translation: AAH24887.1.
BC034881 mRNA. Translation: AAH34881.1. Different initiation.
CCDSiCCDS16019.1. [Q811M1-1]
RefSeqiNP_722542.2. NM_153820.3. [Q811M1-1]
XP_006498462.1. XM_006498399.2.
UniGeneiMm.217350.

Genome annotation databases

EnsembliENSMUST00000055776; ENSMUSP00000056461; ENSMUSG00000049744. [Q811M1-1]
ENSMUST00000112822; ENSMUSP00000108441; ENSMUSG00000049744. [Q811M1-4]
GeneIDi76117.
KEGGimmu:76117.
UCSCiuc008jou.2. mouse. [Q811M1-3]
uc008jov.1. mouse. [Q811M1-2]
uc008jow.1. mouse. [Q811M1-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK031022 mRNA. Translation: BAC27217.1.
AK170537 mRNA. Translation: BAE41865.1.
AK172407 mRNA. Translation: BAE42992.1.
AL732320
, AL844535, AL845160, AL928611, AL935152 Genomic DNA. Translation: CAM15030.1.
AL844535
, AL732320, AL845160, AL928611, AL935152 Genomic DNA. Translation: CAM17611.1.
AL928611
, AL732320, AL844535, AL845160, AL935152 Genomic DNA. Translation: CAM19036.1.
AL845160
, AL732320, AL844535, AL928611, AL935152 Genomic DNA. Translation: CAM20252.1.
AL935152
, AL732320, AL844535, AL845160, AL928611 Genomic DNA. Translation: CAM27855.1.
BC024887 mRNA. Translation: AAH24887.1.
BC034881 mRNA. Translation: AAH34881.1. Different initiation.
CCDSiCCDS16019.1. [Q811M1-1]
RefSeqiNP_722542.2. NM_153820.3. [Q811M1-1]
XP_006498462.1. XM_006498399.2.
UniGeneiMm.217350.

3D structure databases

ProteinModelPortaliQ811M1.
SMRiQ811M1. Positions 88-199, 268-476.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi217972. 2 interactions.
STRINGi10090.ENSMUSP00000056461.

PTM databases

iPTMnetiQ811M1.
PhosphoSiteiQ811M1.

Proteomic databases

EPDiQ811M1.
MaxQBiQ811M1.
PaxDbiQ811M1.
PRIDEiQ811M1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000055776; ENSMUSP00000056461; ENSMUSG00000049744. [Q811M1-1]
ENSMUST00000112822; ENSMUSP00000108441; ENSMUSG00000049744. [Q811M1-4]
GeneIDi76117.
KEGGimmu:76117.
UCSCiuc008jou.2. mouse. [Q811M1-3]
uc008jov.1. mouse. [Q811M1-2]
uc008jow.1. mouse. [Q811M1-1]

Organism-specific databases

CTDi55843.
MGIiMGI:1923367. Arhgap15.

Phylogenomic databases

eggNOGiKOG1449. Eukaryota.
KOG1450. Eukaryota.
ENOG410ZP6T. LUCA.
GeneTreeiENSGT00840000129684.
HOGENOMiHOG000294167.
HOVERGENiHBG005328.
InParanoidiQ811M1.
OMAiQDNATRI.
PhylomeDBiQ811M1.
TreeFamiTF329345.

Enzyme and pathway databases

ReactomeiR-MMU-194840. Rho GTPase cycle.

Miscellaneous databases

PROiQ811M1.
SOURCEiSearch...

Gene expression databases

BgeeiQ811M1.
CleanExiMM_ARHGAP15.
ExpressionAtlasiQ811M1. baseline and differential.
GenevisibleiQ811M1. MM.

Family and domain databases

Gene3Di1.10.555.10. 1 hit.
2.30.29.30. 1 hit.
InterProiIPR011993. PH_dom-like.
IPR001849. PH_domain.
IPR015767. Rho_GTPase_act.
IPR008936. Rho_GTPase_activation_prot.
IPR000198. RhoGAP_dom.
[Graphical view]
PANTHERiPTHR23181. PTHR23181. 1 hit.
PfamiPF00169. PH. 1 hit.
PF00620. RhoGAP. 1 hit.
[Graphical view]
SMARTiSM00233. PH. 1 hit.
SM00324. RhoGAP. 1 hit.
[Graphical view]
SUPFAMiSSF48350. SSF48350. 1 hit.
SSF50729. SSF50729. 1 hit.
PROSITEiPS50003. PH_DOMAIN. 1 hit.
PS50238. RHOGAP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2; 3 AND 4).
    Strain: C57BL/6J and NOD.
    Tissue: Spleen and Thymus.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: FVB/N.
    Tissue: Mammary tumor.
  4. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-204, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Lung and Spleen.

Entry informationi

Entry nameiRHG15_MOUSE
AccessioniPrimary (citable) accession number: Q811M1
Secondary accession number(s): Q3T9M8
, Q3TCU0, Q8C0I5, Q8JZY0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: June 1, 2003
Last modified: June 8, 2016
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.