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Protein

Microtubule-associated serine/threonine-protein kinase 4

Gene

Mast4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.By similarity

Cofactori

Mg2+By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei597 – 5971ATPPROSITE-ProRule annotationBy similarity
Active sitei691 – 6911Proton acceptorPROSITE-ProRule annotationBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi574 – 5829ATPPROSITE-ProRule annotationBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.11.1. 3474.

Names & Taxonomyi

Protein namesi
Recommended name:
Microtubule-associated serine/threonine-protein kinase 4 (EC:2.7.11.1)
Gene namesi
Name:Mast4Imported
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 13

Organism-specific databases

MGIiMGI:1918885. Mast4.

Subcellular locationi

  • Cytoplasm By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 26182618Microtubule-associated serine/threonine-protein kinase 4PRO_0000293629Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei204 – 2041PhosphoserineCombined sources
Modified residuei211 – 2111PhosphoserineCombined sources
Modified residuei268 – 2681PhosphoserineBy similarity
Modified residuei912 – 9121PhosphoserineBy similarity
Modified residuei1290 – 12901PhosphoserineCombined sources
Modified residuei1368 – 13681PhosphoserineCombined sources
Modified residuei1382 – 13821PhosphoserineBy similarity
Modified residuei1395 – 13951PhosphoserineCombined sources
Modified residuei1417 – 14171PhosphoserineBy similarity
Modified residuei1465 – 14651PhosphoserineBy similarity
Modified residuei1521 – 15211PhosphoserineBy similarity
Modified residuei1776 – 17761PhosphoserineBy similarity
Modified residuei1819 – 18191PhosphoserineBy similarity
Modified residuei1906 – 19061PhosphoserineBy similarity
Modified residuei2439 – 24391PhosphoserineBy similarity
Modified residuei2517 – 25171PhosphoserineBy similarity
Modified residuei2549 – 25491PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ811L6.
PaxDbiQ811L6.
PeptideAtlasiQ811L6.
PRIDEiQ811L6.

PTM databases

iPTMnetiQ811L6.
PhosphoSiteiQ811L6.

Expressioni

Gene expression databases

BgeeiENSMUSG00000034751.
ExpressionAtlasiQ811L6. baseline and differential.
GenevisibleiQ811L6. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000128464.

Structurei

3D structure databases

ProteinModelPortaliQ811L6.
SMRiQ811L6. Positions 379-479, 1138-1229.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini568 – 841274Protein kinasePROSITE-ProRule annotationAdd
BLAST
Domaini842 – 90463AGC-kinase C-terminalSequence analysisAdd
BLAST
Domaini1139 – 122789PDZPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 AGC-kinase C-terminal domain.Sequence analysis
Contains 1 PDZ (DHR) domain.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0606. Eukaryota.
ENOG410XPWX. LUCA.
GeneTreeiENSGT00530000063286.
HOGENOMiHOG000046662.
HOVERGENiHBG108118.
InParanoidiQ811L6.
KOiK08789.
OMAiECLPGNP.
OrthoDBiEOG091G00IH.
TreeFamiTF313149.

Family and domain databases

Gene3Di1.20.1480.20. 1 hit.
2.30.42.10. 1 hit.
InterProiIPR000961. AGC-kinase_C.
IPR011009. Kinase-like_dom.
IPR015022. MA_Ser/Thr_Kinase_dom.
IPR023142. MAST_pre-PK_dom.
IPR001478. PDZ.
IPR000719. Prot_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF08926. DUF1908. 1 hit.
PF00595. PDZ. 1 hit.
PF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00228. PDZ. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF140482. SSF140482. 1 hit.
SSF50156. SSF50156. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS51285. AGC_KINASE_CTER. 1 hit.
PS50106. PDZ. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 13 Publications (identifier: Q811L6-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGEKVSEAPE PVPRGCSGHG ARTLVSSAAA VSSEGASSAE SSSGSETLSE
60 70 80 90 100
EGEPSRFSCR SQPPRPPGGA LGTRLPAAWA PARVALERGV PTLPLPHPGG
110 120 130 140 150
AVLPVPQVSS ASQEEQDEEL DHILSPPPMP FRKCSNPDVA CGLGKSLKYK
160 170 180 190 200
RQLSEDGKQL RRGSLGGALT GRYLLPNPVA GQAWPASAET SNLVRMRSQA
210 220 230 240 250
LGQSAPSLTA SLKELSLPRR GSLCRTSNRK SLIGNGQSPA LPRPHSPLSA
260 270 280 290 300
HAGNSPQDSP RNFSPSASAH FSFARRTDGR RWSLASLPSS GYGTNTPSST
310 320 330 340 350
VSSSCSSQEK LHQLPYQPTP DELHFLSKHF CTTESIATEN RCRNTPMRPR
360 370 380 390 400
SRSLSPGRSP ACCDHEIIMM NHVYKERFPK ATAQMEERLK EIITSYSPDH
410 420 430 440 450
VLPLADGVLS FTHHQIIELA RDCLDKSHQG LITSRYFFEL QHKLDKLLQE
460 470 480 490 500
AHDRSESGEL AFIKQLVRKI LIVIARPARL LECLEFDPEE FYYLLEAAEG
510 520 530 540 550
HAKEGQGIKT DIPRYIISQL GLNKDPLEEM AQLGNYDSRT AETPEMDESV
560 570 580 590 600
SSSNTSLRLR RKPRESDFET IKLISNGAYG AVYFVRHKES RQRFAMKKIN
610 620 630 640 650
KQNLILRNQI QQAFVERDIL TFAENPFVVS MYCSFETRRH LCMVMEYVEG
660 670 680 690 700
GDCATLMKNM GPLPVDMARM YFAETVLALE YLHNYGIVHR DLKPDNLLVT
710 720 730 740 750
SMGHIKLTDF GLSKVGLMSM TTNLYEGHIE KDAREFLDKQ VCGTPEYIAP
760 770 780 790 800
EVILRQGYGK PVDWWAMGII LYEFLVGCVP FFGDTPEELF GQVISDEINW
810 820 830 840 850
PEKDEAPPPD AQELITLLLR QNPLERLGTG GAYEVKQHRF FRSLDWNSLL
860 870 880 890 900
RQKAEFIPQL ESEDDTSYFD TRSEKYHHME TEEEDDTNDE DFTVEIRQFS
910 920 930 940 950
SCSHRFSKVF SSIDRITQNS GEDKDDSEDK TKSTTLPSTE TLSWSSEYSE
960 970 980 990 1000
MQQLSTSNSS DTESNRCKLS SGLLPKLAIS TDGEQDEAVP CSGDPREEPE
1010 1020 1030 1040 1050
KPVPPSEECT QEEPEVTTPA STISSSTLSV GSFSEHLDQI NGRSECVDST
1060 1070 1080 1090 1100
DNSSKPSSEP TSHVARQRLE STEKKKISGK VTKSLSASAL SLMIPGDMFA
1110 1120 1130 1140 1150
VSPLGSPMSP HSLSSDPSSS RDSSPSRDSS AASASPHQPI VIHSSGKNYG
1160 1170 1180 1190 1200
FTIRAIRVYV GDSDIYTVHH IVWNVEEGSP AYQAGLKAGD LITHINGEPV
1210 1220 1230 1240 1250
HGLVHTEVIE LLLKSGNKVS ITTTPFENTS IKTGPARRNS YKGRMVRRSK
1260 1270 1280 1290 1300
KSKKKESLER RRSLFKKLAK QPSPLLHTSR SFSCLNRSLS SGESLPGSPT
1310 1320 1330 1340 1350
HSLSPRSPTP SYRSTPDFPS GTNSSQSSSP SSSAPNSPAG SGHIRPSTLH
1360 1370 1380 1390 1400
GLAPKLSGQR YRSGRRKSAG SIPLSPLART PSPTPQPTSP QRSPSPLLGH
1410 1420 1430 1440 1450
SLGNAKITQA FPSKMHSPPT IVRHIVRPKS AEPPRSPLLK RVQSEEKLSP
1460 1470 1480 1490 1500
SYGSDKKLLC SRKHSLEVTQ EEVQREQCQR EVTLQSLEEN VCDAPSLSRA
1510 1520 1530 1540 1550
RPVEQGCLKR PVSRKVGRQE SVDDLDRDKL KAKVVVKKPE EKHESHQKPH
1560 1570 1580 1590 1600
SLGGDSESYA LFRLEEREKK VYSKGLERSG HFENTSAELP SVGSLLKDTL
1610 1620 1630 1640 1650
HKQASVRASE GVTSDGAACS LTPGEHSQSL GDFKRASASG ILHDSVCPIS
1660 1670 1680 1690 1700
DRPAPGKVEY SEKASQAKEL LRSEKLDSKL ANIDYLRKKM SLDDKDDSHC
1710 1720 1730 1740 1750
AILKPKITSS AHECLPGNPI RPMAGQQETP PASENRAFIN STHTPQMSAV
1760 1770 1780 1790 1800
SFVPLKALAG RVENGGEKAG LAAPESPVRK SPSEYKLEGR SVSCLKPIEG
1810 1820 1830 1840 1850
TLDIALLSGP HASKTELLSP EPAQSPSPGI NVGPCVPLAL PGSSGKKGDS
1860 1870 1880 1890 1900
TSLREPSSAN LKVNKSYLLE PRFLPPSRAL QDSLAASGPE PKSKPERKLI
1910 1920 1930 1940 1950
HPSARSPATV TESNLQQKEG GPATHQDRST DTRNLPGPGQ TLHNVDLPRL
1960 1970 1980 1990 2000
CTRAPLPPEG TPAKEKPCLK EPSAKVKSEW SAVRDDGHRD PCAKLCPAET
2010 2020 2030 2040 2050
GKASDSSKPL PSGGRTQPDF YKQTQTSEKA WAHAKTNHKD SQDEVKSLAR
2060 2070 2080 2090 2100
EDSASLLYEK EIGRARKGPE PKPEVPATRC PPQPPGIEGE KREKLSAAPS
2110 2120 2130 2140 2150
LQKQAPKEPD RKEQTSQRPG GSGPQQPPPT KELSNSASWQ HGSSPSHTLK
2160 2170 2180 2190 2200
KEPGTKAAAA EPSTSLHDTP RSATATTTAI ATTTTTTSAG HSDCSSHKAR
2210 2220 2230 2240 2250
PGPDPSPSKS KHQDRSLSSQ KLSAGSAKGK EPVTQPLGGS IREGKGGSKG
2260 2270 2280 2290 2300
PVDTFSAVLT TQGKASDVLV QGEGRVSIIV HTEECPLDAK LKNTNGGCPP
2310 2320 2330 2340 2350
EMQAKHPPRQ GHLSEAADQK PLIAGEKQSP SPKHPKPSTV KDYPSLCRQT
2360 2370 2380 2390 2400
DRSPSHQATT GDRKAEGKKC TDALYVAAPE GYKPEASPSL HHGETGLRGS
2410 2420 2430 2440 2450
ERPPMGMGKG FSEPKGKGPG PQKSLAETGK PSGMKRSPSA TVQSSLRSAA
2460 2470 2480 2490 2500
PPEKSLSYSA SFPEAQPGVR EVPAANSSPS SAKATGGTSE FPAPSSRDHR
2510 2520 2530 2540 2550
KLQSGGDGRS QMIKSDSLPS FRLSTSALES HFQDPQVPIA SGHRGRALSV
2560 2570 2580 2590 2600
TAATGEPKGR ELAQPPPVRK QNACREATRA PPAPSTDRSL PLSSEKDFVV
2610
RQRRGKETLR SSPHKKAS
Note: No experimental confirmation available.Curated
Length:2,618
Mass (Da):284,004
Last modified:July 27, 2011 - v3
Checksum:i493D6B86DA86BA5C
GO
Isoform 21 Publication (identifier: Q811L6-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     350-521: Missing.
     1030-1096: Missing.
     1263-1282: SLFKKLAKQPSPLLHTSRSF → DRKKKKKRELSSRCLPSSNR
     1283-2618: Missing.

Note: No experimental confirmation available.Curated
Show »
Length:1,043
Mass (Da):115,187
Checksum:iA5ED609C263092EF
GO

Sequence cautioni

The sequence AK090136 differs from that shown. Reason: Frameshift at position 1682. 1 Publication

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti546 – 5461M → T in AAH42511 (PubMed:15489334).Curated
Sequence conflicti1963 – 19631A → T in BAC97924 (PubMed:14621295).Curated
Sequence conflicti1977 – 19771K → I in BAC28008 (PubMed:16141072).Curated
Sequence conflicti2143 – 21431S → G in BAC28008 (PubMed:16141072).Curated
Sequence conflicti2181 – 21811A → ATA in BAC97924 (PubMed:14621295).Curated
Sequence conflicti2444 – 24441S → G in BAC28008 (PubMed:16141072).Curated
Sequence conflicti2507 – 25071D → E in BAE23322 (PubMed:16141072).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei350 – 521172Missing in isoform 2. 1 PublicationVSP_041616Add
BLAST
Alternative sequencei1030 – 109667Missing in isoform 2. 1 PublicationVSP_052478Add
BLAST
Alternative sequencei1263 – 128220SLFKK…TSRSF → DRKKKKKRELSSRCLPSSNR in isoform 2. 1 PublicationVSP_052479Add
BLAST
Alternative sequencei1283 – 26181336Missing in isoform 2. 1 PublicationVSP_052480Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK032755 mRNA. Translation: BAC28008.1.
AK090136 mRNA. No translation available.
AK137360 mRNA. Translation: BAE23322.1.
AC112791 Genomic DNA. No translation available.
AC114827 Genomic DNA. No translation available.
AC124387 Genomic DNA. No translation available.
AC161446 Genomic DNA. No translation available.
AC165290 Genomic DNA. No translation available.
AC167975 Genomic DNA. No translation available.
BC042511 mRNA. Translation: AAH42511.1.
AK129114 mRNA. Translation: BAC97924.1.
Z78145 mRNA. Translation: CAB01547.1.
CCDSiCCDS49349.1. [Q811L6-1]
RefSeqiNP_780380.2. NM_175171.3. [Q811L6-1]
XP_011242972.1. XM_011244670.1. [Q811L6-1]
UniGeneiMm.202606.
Mm.447520.

Genome annotation databases

EnsembliENSMUST00000167058; ENSMUSP00000128464; ENSMUSG00000034751. [Q811L6-1]
GeneIDi328329.
KEGGimmu:328329.
UCSCiuc007rsb.2. mouse. [Q811L6-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK032755 mRNA. Translation: BAC28008.1.
AK090136 mRNA. No translation available.
AK137360 mRNA. Translation: BAE23322.1.
AC112791 Genomic DNA. No translation available.
AC114827 Genomic DNA. No translation available.
AC124387 Genomic DNA. No translation available.
AC161446 Genomic DNA. No translation available.
AC165290 Genomic DNA. No translation available.
AC167975 Genomic DNA. No translation available.
BC042511 mRNA. Translation: AAH42511.1.
AK129114 mRNA. Translation: BAC97924.1.
Z78145 mRNA. Translation: CAB01547.1.
CCDSiCCDS49349.1. [Q811L6-1]
RefSeqiNP_780380.2. NM_175171.3. [Q811L6-1]
XP_011242972.1. XM_011244670.1. [Q811L6-1]
UniGeneiMm.202606.
Mm.447520.

3D structure databases

ProteinModelPortaliQ811L6.
SMRiQ811L6. Positions 379-479, 1138-1229.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000128464.

PTM databases

iPTMnetiQ811L6.
PhosphoSiteiQ811L6.

Proteomic databases

MaxQBiQ811L6.
PaxDbiQ811L6.
PeptideAtlasiQ811L6.
PRIDEiQ811L6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000167058; ENSMUSP00000128464; ENSMUSG00000034751. [Q811L6-1]
GeneIDi328329.
KEGGimmu:328329.
UCSCiuc007rsb.2. mouse. [Q811L6-1]

Organism-specific databases

CTDi375449.
MGIiMGI:1918885. Mast4.

Phylogenomic databases

eggNOGiKOG0606. Eukaryota.
ENOG410XPWX. LUCA.
GeneTreeiENSGT00530000063286.
HOGENOMiHOG000046662.
HOVERGENiHBG108118.
InParanoidiQ811L6.
KOiK08789.
OMAiECLPGNP.
OrthoDBiEOG091G00IH.
TreeFamiTF313149.

Enzyme and pathway databases

BRENDAi2.7.11.1. 3474.

Miscellaneous databases

PROiQ811L6.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000034751.
ExpressionAtlasiQ811L6. baseline and differential.
GenevisibleiQ811L6. MM.

Family and domain databases

Gene3Di1.20.1480.20. 1 hit.
2.30.42.10. 1 hit.
InterProiIPR000961. AGC-kinase_C.
IPR011009. Kinase-like_dom.
IPR015022. MA_Ser/Thr_Kinase_dom.
IPR023142. MAST_pre-PK_dom.
IPR001478. PDZ.
IPR000719. Prot_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF08926. DUF1908. 1 hit.
PF00595. PDZ. 1 hit.
PF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00228. PDZ. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF140482. SSF140482. 1 hit.
SSF50156. SSF50156. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS51285. AGC_KINASE_CTER. 1 hit.
PS50106. PDZ. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMAST4_MOUSE
AccessioniPrimary (citable) accession number: Q811L6
Secondary accession number(s): E9QAH7
, Q3UVE7, Q62489, Q6ZQE0, Q8BME3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 10, 2007
Last sequence update: July 27, 2011
Last modified: September 7, 2016
This is version 115 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.