ID   ATG4C_MOUSE             Reviewed;         458 AA.
AC   Q811C2; B1ASJ9; Q6PD25;
DT   15-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT   27-JUL-2011, sequence version 2.
DT   16-NOV-2011, entry version 73.
DE   RecName: Full=Cysteine protease ATG4C;
DE            EC=3.4.22.-;
DE   AltName: Full=AUT-like 3 cysteine endopeptidase;
DE   AltName: Full=Autophagin-3;
DE   AltName: Full=Autophagy-related cysteine endopeptidase 3;
DE   AltName: Full=Autophagy-related protein 4 homolog C;
GN   Name=Atg4c; Synonyms=Apg4c, Autl3;
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi;
OC   Muroidea; Muridae; Murinae; Mus; Mus.
OX   NCBI_TaxID=10090;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Brain;
RX   PubMed=12446702; DOI=10.1074/jbc.M208247200;
RA   Marino G., Uria J.A., Puente X.S., Quesada V., Bordallo J.,
RA   Lopez-Otin C.;
RT   "Human autophagins, a family of cysteine proteinases potentially
RT   implicated in cell degradation by autophagy.";
RL   J. Biol. Chem. 278:3671-3678(2003).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=C57BL/6;
RX   PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA   Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S.,
RA   She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W.,
RA   Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T.,
RA   Zhou S., Teague B., Potamousis K., Churas C., Place M., Herschleb J.,
RA   Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z.,
RA   Lindblad-Toh K., Eichler E.E., Ponting C.P.;
RT   "Lineage-specific biology revealed by a finished genome assembly of
RT   the mouse.";
RL   PLoS Biol. 7:E1000112-E1000112(2009).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=C57BL/6; TISSUE=Brain;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA
RT   project: the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
CC   -!- FUNCTION: Cysteine protease required for autophagy, which cleaves
CC       the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP,
CC       allowing the liberation of form I. A subpopulation of form I is
CC       subsequently converted to a smaller form (form II). Form II, with
CC       a revealed C-terminal glycine, is considered to be the
CC       phosphatidylethanolamine (PE)-conjugated form, and has the
CC       capacity for the binding to autophagosomes (By similarity).
CC   -!- ENZYME REGULATION: Inhibited by N-ethylmaleimide (By similarity).
CC   -!- SUBCELLULAR LOCATION: Cytoplasm (Probable).
CC   -!- SIMILARITY: Belongs to the peptidase C54 family.
CC   -----------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution-NoDerivs License
CC   -----------------------------------------------------------------------
DR   EMBL; AJ312233; CAC85555.1; -; Genomic_DNA.
DR   EMBL; AL627166; CAM26343.1; -; Genomic_DNA.
DR   EMBL; BC058981; AAH58981.1; -; mRNA.
DR   IPI; IPI00177235; -.
DR   RefSeq; NP_001139439.1; NM_001145967.1.
DR   RefSeq; NP_778194.3; NM_175029.3.
DR   UniGene; Mm.241663; -.
DR   ProteinModelPortal; Q811C2; -.
DR   SMR; Q811C2; 38-426.
DR   STRING; Q811C2; -.
DR   MEROPS; C54.004; -.
DR   PhosphoSite; Q811C2; -.
DR   PRIDE; Q811C2; -.
DR   Ensembl; ENSMUST00000030279; ENSMUSP00000030279; ENSMUSG00000028550.
DR   GeneID; 242557; -.
DR   KEGG; mmu:242557; -.
DR   CTD; 84938; -.
DR   MGI; MGI:2651854; Atg4c.
DR   GeneTree; ENSGT00530000063000; -.
DR   HOGENOM; HBG445086; -.
DR   HOVERGEN; HBG050537; -.
DR   InParanoid; Q811C2; -.
DR   OrthoDB; EOG4GB760; -.
DR   PhylomeDB; Q811C2; -.
DR   NextBio; 457721; -.
DR   ArrayExpress; Q811C2; -.
DR   Bgee; Q811C2; -.
DR   CleanEx; MM_ATG4C; -.
DR   Genevestigator; Q811C2; -.
DR   GermOnline; ENSMUSG00000028550; Mus musculus.
DR   GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR   GO; GO:0004197; F:cysteine-type endopeptidase activity; ISS:UniProtKB.
DR   GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR   GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR   InterPro; IPR005078; Peptidase_C54.
DR   KO; K08342; -.
DR   PANTHER; PTHR22624; Peptidase_C54; 1.
DR   Pfam; PF03416; Peptidase_C54; 1.
PE   2: Evidence at transcript level;
KW   Acetylation; Autophagy; Complete proteome; Cytoplasm; Hydrolase;
KW   Protease; Protein transport; Reference proteome; Thiol protease;
KW   Transport; Ubl conjugation pathway.
FT   CHAIN         1    458       Cysteine protease ATG4C.
FT                                /FTId=PRO_0000215850.
FT   ACT_SITE    111    111       Nucleophile (By similarity).
FT   ACT_SITE    345    345       Potential.
FT   ACT_SITE    347    347       By similarity.
FT   MOD_RES       1      1       N-acetylmethionine (By similarity).
FT   CONFLICT     87     87       I -> L (in Ref. 3; AAH58981).
FT   CONFLICT    190    190       A -> T (in Ref. 1; CAC85555).
SQ   SEQUENCE   458 AA;  52056 MW;  E9F1C1A697CA4978 CRC64;
     MEASGTDEVD KLKTKFISAW NNMKYSWVLK TKTYFSRNSP VLLLGKCYHF KYEDESKMLP
     ARSGCAIEDH VIAGNVEEFR KDFISRIWLT YREEFPQIEA SALTTDCGWG CTLRTGQMLL
     AQGLILHFLG RAWTWPDALH IENADSDSWT SNTVKKFTAS FEASLSGDRE LRTPAVSLKE
     TSGKCPDDHA VRNEAYHRKI ISWFGDSPVA VFGLHRLIEF GKKSGKKAGD WYGPAVVAHI
     LRKAVEEARH PDLQGLTIYV AQDCTVYNSD VIDKQTDSVT AGDARDKAVI ILVPVRLGGE
     RTNTDYLEFV KGVLSLEYCV GIIGGKPKQS YYFAGFQDDS LIYMDPHYCQ SFVDVSIKDF
     PLETFHCPSP KKMSFRKMDP SCTIGFYCRN VQDFERASEE ITKMLKISSK EKYPLFTFVN
     GHSKDFDFTS TAASEEDLFS EDERKNFKRF STEEFVLL
//