Reviewed, UniProtKB/Swiss-Prot Q811C2 (ATG4C_MOUSE)

Names and origin

Protein names

Recommended name:
Cysteine protease ATG4C
EC=3.4.22.-

Alternative name(s):
Autophagy-related protein 4 homolog C
Autophagin-3
Autophagy-related cysteine endopeptidase 3
AUT-like 3 cysteine endopeptidase

Gene names

Name:	Atg4c
Synonyms:	Apg4c, Autl3

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	458 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes By similarity.
Enzyme regulation	Inhibited by N-ethylmaleimide By similarity.
Subcellular location	Cytoplasm Probable.
Sequence similarities	Belongs to the peptidase C54 family.

Ontologies

Keywords
Biological process	Autophagy Protein transport Transport Ubl conjugation pathway
Cellular component	Cytoplasm
Molecular function	Hydrolase Protease Thiol protease
PTM	Acetylation
Gene Ontology (GO)
Biological process	autophagy Ref.1 Inferred from sequence or structural similarity. Source: UniProtKB modification-dependent protein catabolic process Inferred from electronic annotation. Source: UniProtKB-KW protein transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Ref.1 Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	cysteine-type endopeptidase activity Ref.1 Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 458	458	Cysteine protease ATG4C		PRO_0000215850
Sites
	Active site	111	1	Nucleophile By similarity
	Active site	345	1	Potential
	Active site	347	1	By similarity
Amino acid modifications
	Modified residue	1	1	N-acetylmethionine By similarity
Experimental info
	Sequence conflict	87	1	I → L in AAH58981. Ref.2
	Sequence conflict	190	1	T → A in AAH58981. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q811C2-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: E6A134A6939A0C78	FASTA	458	52,086
	10 20 30 40 50 60 MEASGTDEVD KLKTKFISAW NNMKYSWVLK TKTYFSRNSP VLLLGKCYHF KYEDESKMLP 70 80 90 100 110 120 ARSGCAIEDH VIAGNVEEFR KDFISRIWLT YREEFPQIEA SALTTDCGWG CTLRTGQMLL 130 140 150 160 170 180 AQGLILHFLG RAWTWPDALH IENADSDSWT SNTVKKFTAS FEASLSGDRE LRTPAVSLKE 190 200 210 220 230 240 TSGKCPDDHT VRNEAYHRKI ISWFGDSPVA VFGLHRLIEF GKKSGKKAGD WYGPAVVAHI 250 260 270 280 290 300 LRKAVEEARH PDLQGLTIYV AQDCTVYNSD VIDKQTDSVT AGDARDKAVI ILVPVRLGGE 310 320 330 340 350 360 RTNTDYLEFV KGVLSLEYCV GIIGGKPKQS YYFAGFQDDS LIYMDPHYCQ SFVDVSIKDF 370 380 390 400 410 420 PLETFHCPSP KKMSFRKMDP SCTIGFYCRN VQDFERASEE ITKMLKISSK EKYPLFTFVN 430 440 450 GHSKDFDFTS TAASEEDLFS EDERKNFKRF STEEFVLL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Human autophagins, a family of cysteine proteinases potentially implicated in cell degradation by autophagy." Marino G., Uria J.A., Puente X.S., Quesada V., Bordallo J., Lopez-Otin C. J. Biol. Chem. 278:3671-3678(2003) [PubMed: 12446702] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Brain.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6. Tissue: Brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ312233 Genomic DNA. Translation: CAC85555.1. BC058981 mRNA. Translation: AAH58981.1.
IPI	IPI00177235.
RefSeq	NP_001139439.1. NP_778194.3. XP_001471904.1.
UniGene	Mm.241663
3D structure databases
SMR	Q811C2. Positions 38-426.
ModBase	Search...
Protein-protein interaction databases
STRING	Q811C2.
Protein family/group databases
MEROPS	C54.004.
PTM databases
PhosphoSite	Q811C2.
Proteomic databases
PRIDE	Q811C2.
Genome annotation databases
Ensembl	ENSMUST00000030279; ENSMUSP00000030279; ENSMUSG00000028550; Mus musculus. [Genome view]
GeneID	100044217. 242557.
KEGG	mmu:100044217. mmu:242557.
UCSC	uc008tuu.1. mouse.
Organism-specific databases
CTD	242557.
MGI	MGI:2651854. Atg4c.
Phylogenomic databases
HOGENOM	HBG445086.
HOVERGEN	HBG050537.
InParanoid	Q811C2.
PhylomeDB	Q811C2.
Gene expression databases
ArrayExpress	Q811C2.
Bgee	Q811C2.
CleanEx	MM_ATG4C.
Genevestigator	Q811C2.
GermOnline	ENSMUSG00000028550. Mus musculus.
Family and domain databases
InterPro	IPR005078. Peptidase_C54. [Graphical view]
PANTHER	PTHR22624. Peptidase_C54. 1 hit.
Pfam	PF03416. Peptidase_C54. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	457721.
SOURCE	Search...

Entry information

Entry name

ATG4C_MOUSE

Accession

Primary (citable) accession number: Q811C2
Secondary accession number(s): Q6PD25

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2005
Last sequence update:	June 1, 2003
Last modified:	March 2, 2010
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)