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Q811B3

- ATS12_MOUSE

UniProt

Q811B3 - ATS12_MOUSE

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Protein

A disintegrin and metalloproteinase with thrombospondin motifs 12

Gene
Adamts12
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

Metalloprotease that plays a role in the degradation of COMP By similarity. Cleaves also alpha-2 macroglobulin and aggregan. Has anti-tumorigenic properties By similarity.

Cofactori

Binds 1 zinc ion per subunit By similarity.

Enzyme regulationi

Inhibited by alpha-2 macroglobulin By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi212 – 2121Zinc; in inhibited form By similarity
Metal bindingi396 – 3961Zinc; catalytic By similarity
Active sitei397 – 3971 By similarity
Metal bindingi400 – 4001Zinc; catalytic By similarity
Metal bindingi406 – 4061Zinc; catalytic By similarity

GO - Molecular functioni

  1. metalloendopeptidase activity Source: InterPro
  2. zinc ion binding Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Metalloprotease, Protease

Keywords - Ligandi

Metal-binding, Zinc

Protein family/group databases

MEROPSiM12.237.

Names & Taxonomyi

Protein namesi
Recommended name:
A disintegrin and metalloproteinase with thrombospondin motifs 12 (EC:3.4.24.-)
Short name:
ADAM-TS 12
Short name:
ADAM-TS12
Short name:
ADAMTS-12
Gene namesi
Name:Adamts12
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 15

Organism-specific databases

MGIiMGI:2146046. Adamts12.

Subcellular locationi

GO - Cellular componenti

  1. proteinaceous extracellular matrix Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Extracellular matrix, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2525 Reviewed predictionAdd
BLAST
Propeptidei26 – 244219 By similarityPRO_0000029188Add
BLAST
Chaini245 – 16001356A disintegrin and metalloproteinase with thrombospondin motifs 12PRO_0000029189Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi105 – 1051N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi326 ↔ 380 By similarity
Disulfide bondi355 ↔ 362 By similarity
Disulfide bondi374 ↔ 455 By similarity
Disulfide bondi413 ↔ 439 By similarity
Disulfide bondi482 ↔ 505 By similarity
Disulfide bondi493 ↔ 511 By similarity
Disulfide bondi500 ↔ 530 By similarity
Disulfide bondi524 ↔ 535 By similarity
Disulfide bondi558 ↔ 595 By similarity
Disulfide bondi562 ↔ 600 By similarity
Disulfide bondi573 ↔ 585 By similarity

Post-translational modificationi

The precursor is cleaved by a furin endopeptidase By similarity.
Subjected to an intracellular maturation process yielding a 120 kDa N-terminal fragment containing the metalloproteinase, disintegrin, one TSP type-1 and the Cys-rich domains and a 83 kDa C-terminal fragment containing the spacer 2 and four TSP type-1 domains By similarity.
Glycosylated. Can be O-fucosylated by POFUT2 on a serine or a threonine residue found within the consensus sequence C1-X(2)-(S/T)-C2-G of the TSP type-1 repeat domains where C1 and C2 are the first and second cysteine residue of the repeat, respectively. Fucosylated repeats can then be further glycosylated by the addition of a beta-1,3-glucose residue by the glucosyltransferase, B3GALTL. Fucosylation mediates the efficient secretion of ADAMTS family members. Also can be C-glycosylated with one or two mannose molecules on tryptophan residues within the consensus sequence W-X-X-W of the TPRs, and N-glycosylated. These other glycosylations can also facilitate secretion By similarity.

Keywords - PTMi

Cleavage on pair of basic residues, Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

PRIDEiQ811B3.

PTM databases

PhosphoSiteiQ811B3.

Expressioni

Gene expression databases

BgeeiQ811B3.
GenevestigatoriQ811B3.

Interactioni

Subunit structurei

Interacts with COMP By similarity.

Structurei

3D structure databases

ProteinModelPortaliQ811B3.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini250 – 460211Peptidase M12BAdd
BLAST
Domaini469 – 54880DisintegrinAdd
BLAST
Domaini546 – 60156TSP type-1 1Add
BLAST
Domaini827 – 88761TSP type-1 2Add
BLAST
Domaini891 – 94757TSP type-1 3Add
BLAST
Domaini948 – 100154TSP type-1 4Add
BLAST
Domaini1318 – 137154TSP type-1 5Add
BLAST
Domaini1373 – 142856TSP type-1 6Add
BLAST
Domaini1429 – 147749TSP type-1 7Add
BLAST
Domaini1478 – 153861TSP type-1 8Add
BLAST
Domaini1541 – 158141PLACAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni705 – 831127Spacer 1Add
BLAST
Regioni1001 – 1321321Spacer 2Add
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi210 – 2178Cysteine switch By similarity

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi306 – 3094Poly-Glu
Compositional biasi601 – 704104Cys-richAdd
BLAST

Domaini

The C-terminal four TSP1-like repeats are necessary and sufficient for binding COMP By similarity.
The spacer domain and the TSP type-1 domains are important for a tight interaction with the extracellular matrix By similarity.
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.

Sequence similaritiesi

Contains 1 disintegrin domain.
Contains 1 PLAC domain.
Contains 8 TSP type-1 domains.

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiNOG237764.
GeneTreeiENSGT00750000117354.
HOGENOMiHOG000015092.
HOVERGENiHBG050620.
InParanoidiQ811B3.
KOiK08626.
OMAiPFIMSRQ.
OrthoDBiEOG7V765X.
TreeFamiTF313537.

Family and domain databases

Gene3Di3.40.390.10. 1 hit.
InterProiIPR010294. ADAM_spacer1.
IPR024079. MetalloPept_cat_dom.
IPR001590. Peptidase_M12B.
IPR013273. Peptidase_M12B_ADAM-TS.
IPR002870. Peptidase_M12B_N.
IPR010909. PLAC.
IPR000884. Thrombospondin_1_rpt.
[Graphical view]
PfamiPF05986. ADAM_spacer1. 1 hit.
PF01562. Pep_M12B_propep. 1 hit.
PF01421. Reprolysin. 1 hit.
PF00090. TSP_1. 7 hits.
[Graphical view]
PRINTSiPR01857. ADAMTSFAMILY.
SMARTiSM00209. TSP1. 8 hits.
[Graphical view]
SUPFAMiSSF82895. SSF82895. 8 hits.
PROSITEiPS50215. ADAM_MEPRO. 1 hit.
PS50900. PLAC. 1 hit.
PS50092. TSP1. 6 hits.
PS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q811B3-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MPCARGSWLA KLSIVAQLIN FGAFCHGRQT QPWPVRFPDP RQEHFIKSLP     50
EYHIVSPVQV DAGGHVLSYG LHHPVTSSRK KRAAGGSGDQ LYYRISHEEK 100
DLFFNLTVNW EFLSNGYVVE KRYGNLSHVK MVASSGQPCH LRGTVLQQGT 150
TVGIGTAALS ACQGLTGFFH LPHGDFFIEP VKKHPLTEEG SYPHVVYRRQ 200
SIRAPETKEP ICGLKDSLDN SVKQELQREK WERKTLRSRS LSRRSISKER 250
WVETLVVADT KTVEYHGSEN VESYILTIMN MVTGLFHSPS IGNLVHIVVV 300
RLILLEEEEQ GLKIVHHAEK TLSSFCKWQK SINPKSDLNP VHHDVAVLIT 350
RKDICAGVNR PCETLGLSQL SGMCQPHRSC NINEDSGLPL AFTIAHELGH 400
SFGIQHDGKE NDCEPVGRHP YIMSQQIQYD PTPLTWSKCS KEYITRFLDR 450
GRGFCLDDIP SKKGLKSNVI APGVIYDVHH QCQLQYGPNA TFCQEVENVC 500
QTLWCSVKGF CRSKLDAAAD GTRCGEKKWC MAGKCITVGK KPESIPGGWG 550
RWSPWSHCSR TCGAGAQSAE RLCNNPEPKF GGKYCTGERK RYRLCNVHPC 600
RSDTPTFRQM QCSEFDTVPY KNQFYRWFPV FNAAHPCELY CRPIDEQFSE 650
RMLEAVIDGT PCFEGGNSRN VCINGICKRV GCDYEIDSNA TEDRCGVCLG 700
DGSACQTVKK LFRQKEGSGY VDIGLIPKGA RDIRVMEIKA AGNFLAIRSE 750
DPEKYYLNGG FIIQWNGNYK LAGTVFQYDR KGDLEKLIAP GPTNESVWLQ 800
LLFQVTNPGI KYEYTVRKDG LDNDVEKLLY FWQFGRWTEC SVTCGTGIRR 850
QAAHCVKKGH GIVKTTFCNP ETQPSVRQKK CHEKDCPPRW WAGEWEACST 900
TCGPYGEKKR TVLCIQTMGS DEQALPATDC QHLLKPKALV SCNRDILCPS 950
DWTVGNWSEC SVSCGGGVRI RSVTCAKNLN EPCDKTRKPN SRALCGLQQC 1000
PFSRRVLKPN KDIAPSGKNQ STAEHDPFKP IPAPTSRPTP LSTPTVPESM 1050
STSTPTINSL GSTIASQEDA NGMGWQNNST QAEEGSHFPT SSGSTSQVPV 1100
TSWSLSIQPD DENVSSSAIG PTSEGDFWAT TTSDSGLSSS DAMTWQVTPF 1150
YSTMTTDPEV EIHSGSGEDS DQPLNKDKSN SVIWNKIGVP EHDAPMETDA 1200
ELPLGPPPTS YMGEEPSWPP FSTKMEGSLP AWSFKNETPR DDGMIAEKSR 1250
KIPLPLAGDH HPATSEKLEN HDKLALPNTT NPTQGFGPVL TEEDASNLIA 1300
EGFLLNASDY KHLMKDHSPA YWIVGNWSKC STTCGLGAYW RSVECSSGVD 1350
ADCTTIQRPD PAKKCHLRPC AGWRVGNWSK CSRNCSGGFK IREVQCMDSL 1400
DHHRSLRPFH CQFLAGAPPP LSMSCNLEPC GEWQVEPWSQ CSRSCGGGVQ 1450
ERGVSCPGGL CDWTKRPATT VPCNRHLCCH WATGNWELCN TSCGGGSQKR 1500
TIHCIPSENS TTEDQDQCLC DHQVKPPEFQ TCNQQACRKS ADLTCLKDRL 1550
SISFCQTLKS MRKCSVPSVR AQCCLSCPQA PSIHTQRQRK QQLLQNHDML 1600
Length:1,600
Mass (Da):177,769
Last modified:July 27, 2011 - v2
Checksum:iF125753A42D4AD04
GO
Isoform 2 (identifier: Q811B3-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     167-171: GFFHL → VLITL
     172-1600: Missing.

Note: No experimental confirmation available.

Show »
Length:171
Mass (Da):18,843
Checksum:i6846F3A8C242B832
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei167 – 1715GFFHL → VLITL in isoform 2. VSP_013149
Alternative sequencei172 – 16001429Missing in isoform 2. VSP_013150Add
BLAST

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti1010 – 10101N → H in CAD60967. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ537452 mRNA. Translation: CAD60967.1.
AK048612 mRNA. Translation: BAC33391.1.
AK054015 mRNA. Translation: BAC35621.1.
AC102219 Genomic DNA. No translation available.
AC156609 Genomic DNA. No translation available.
AC159963 Genomic DNA. No translation available.
CCDSiCCDS27384.1. [Q811B3-1]
RefSeqiNP_780710.2. NM_175501.2. [Q811B3-1]
UniGeneiMm.482727.

Genome annotation databases

EnsembliENSMUST00000061318; ENSMUSP00000057796; ENSMUSG00000047497. [Q811B3-1]
GeneIDi239337.
KEGGimmu:239337.
UCSCiuc007vha.1. mouse. [Q811B3-2]
uc007vhb.1. mouse. [Q811B3-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ537452 mRNA. Translation: CAD60967.1 .
AK048612 mRNA. Translation: BAC33391.1 .
AK054015 mRNA. Translation: BAC35621.1 .
AC102219 Genomic DNA. No translation available.
AC156609 Genomic DNA. No translation available.
AC159963 Genomic DNA. No translation available.
CCDSi CCDS27384.1. [Q811B3-1 ]
RefSeqi NP_780710.2. NM_175501.2. [Q811B3-1 ]
UniGenei Mm.482727.

3D structure databases

ProteinModelPortali Q811B3.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

MEROPSi M12.237.

PTM databases

PhosphoSitei Q811B3.

Proteomic databases

PRIDEi Q811B3.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000061318 ; ENSMUSP00000057796 ; ENSMUSG00000047497 . [Q811B3-1 ]
GeneIDi 239337.
KEGGi mmu:239337.
UCSCi uc007vha.1. mouse. [Q811B3-2 ]
uc007vhb.1. mouse. [Q811B3-1 ]

Organism-specific databases

CTDi 81792.
MGIi MGI:2146046. Adamts12.

Phylogenomic databases

eggNOGi NOG237764.
GeneTreei ENSGT00750000117354.
HOGENOMi HOG000015092.
HOVERGENi HBG050620.
InParanoidi Q811B3.
KOi K08626.
OMAi PFIMSRQ.
OrthoDBi EOG7V765X.
TreeFami TF313537.

Miscellaneous databases

NextBioi 384074.
PROi Q811B3.
SOURCEi Search...

Gene expression databases

Bgeei Q811B3.
Genevestigatori Q811B3.

Family and domain databases

Gene3Di 3.40.390.10. 1 hit.
InterProi IPR010294. ADAM_spacer1.
IPR024079. MetalloPept_cat_dom.
IPR001590. Peptidase_M12B.
IPR013273. Peptidase_M12B_ADAM-TS.
IPR002870. Peptidase_M12B_N.
IPR010909. PLAC.
IPR000884. Thrombospondin_1_rpt.
[Graphical view ]
Pfami PF05986. ADAM_spacer1. 1 hit.
PF01562. Pep_M12B_propep. 1 hit.
PF01421. Reprolysin. 1 hit.
PF00090. TSP_1. 7 hits.
[Graphical view ]
PRINTSi PR01857. ADAMTSFAMILY.
SMARTi SM00209. TSP1. 8 hits.
[Graphical view ]
SUPFAMi SSF82895. SSF82895. 8 hits.
PROSITEi PS50215. ADAM_MEPRO. 1 hit.
PS50900. PLAC. 1 hit.
PS50092. TSP1. 6 hits.
PS00142. ZINC_PROTEASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Mouse ADAMTS-12."
    Cal S., Lopez-Otin C.
    Submitted (JAN-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-1009 (ISOFORM 1).
    Strain: C57BL/6J.
    Tissue: Embryonic head and Oviduct.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.

Entry informationi

Entry nameiATS12_MOUSE
AccessioniPrimary (citable) accession number: Q811B3
Secondary accession number(s): E9QKD6, Q8BK92, Q8BKY1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: July 27, 2011
Last modified: July 9, 2014
This is version 91 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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