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Protein

Bone marrow stromal antigen 2

Gene

Bst2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

IFN-induced antiviral host restriction factor which efficiently blocks the release of diverse mammalian enveloped viruses by directly tethering nascent virions to the membranes of infected cells. Acts as a direct physical tether, holding virions to the cell membrane and linking virions to each other. The tethered virions can be internalized by endocytosis and subsequently degraded or they can remain on the cell surface. In either case, their spread as cell-free virions is restricted. Its target viruses belong to diverse families, including retroviridae: human immunodeficiency virus type 1 (HIV-1), mouse mammary tumor virus (MMTV) and murine leukemia virus (MLV), filoviridae: ebola virus (EBOV), arenaviridae: lassa virus (LASV), and rhabdoviridae: vesicular stomatitis virus (VSV). Can inhibit cell surface proteolytic activity of MMP14 causing decreased activation of MMP15 which results in inhibition of cell growth and migration. Can stimulate signaling by LILRA4/ILT7 and consequently provide negative feedback to the production of IFN by plasmacytoid dendritic cells in response to viral infection (By similarity). Plays a role in the organization of the subapical actin cytoskeleton in polarized epithelial cells.By similarity2 Publications

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Immunity, Innate immunity

Names & Taxonomyi

Protein namesi
Recommended name:
Bone marrow stromal antigen 2
Short name:
BST-2
Alternative name(s):
Protein DAMP-1
CD_antigen: CD317
Gene namesi
Name:Bst2
Synonyms:Damp1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi727849. Bst2.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 2626CytoplasmicSequence analysisAdd
BLAST
Transmembranei27 – 4721Helical; Signal-anchor for type II membrane proteinSequence analysisAdd
BLAST
Topological domaini48 – 152105ExtracellularSequence analysisAdd
BLAST

GO - Cellular componenti

  • anchored component of membrane Source: UniProtKB-KW
  • apical plasma membrane Source: UniProtKB
  • cell surface Source: UniProtKB
  • Golgi apparatus Source: UniProtKB-SubCell
  • integral component of membrane Source: GO_Central
  • intracellular Source: RGD
  • late endosome Source: UniProtKB-SubCell
  • membrane raft Source: UniProtKB-SubCell
  • plasma membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Endosome, Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 152152Bone marrow stromal antigen 2PRO_0000253555Add
BLAST
Propeptidei153 – 17220Removed in mature formSequence analysisPRO_0000253556Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Cross-linki20 – 20Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Disulfide bondi58 – 58InterchainBy similarity
Disulfide bondi68 – 68InterchainBy similarity
Glycosylationi70 – 701N-linked (GlcNAc...)Sequence analysis
Disulfide bondi96 – 96InterchainBy similarity
Glycosylationi97 – 971N-linked (GlcNAc...)Sequence analysis
Lipidationi152 – 1521GPI-anchor amidated serineSequence analysis

Post-translational modificationi

N-glycosylated.1 Publication
The GPI anchor is essential for its antiviral activity.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, GPI-anchor, Isopeptide bond, Lipoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ811A2.
PRIDEiQ811A2.

PTM databases

iPTMnetiQ811A2.
PhosphoSiteiQ811A2.

Expressioni

Tissue specificityi

Ubiquitously expressed, with highest levels in brain and liver. Present in liver (at protein level).1 Publication

Interactioni

Subunit structurei

Parallel homodimer; disulfide-linked. May form homotetramers under reducing conditions. Dimerization is essential for its antiviral activity. Interacts with MMP14 (via C-terminal cytoplasmic tail) (By similarity). Interacts with LILRA4/ILT7 (By similarity). Interacts (via cytoplasmic domain) with ARHGAP44.By similarity1 Publication

Protein-protein interaction databases

BioGridi266956. 1 interaction.
STRINGi10116.ENSRNOP00000031515.

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili103 – 14947Sequence analysisAdd
BLAST

Domaini

The extracellular coiled coil domain forms an extended 170 A long semi-flexible rod-like structure important for virion retention at the cell surface and prevention of virus spreading.By similarity

Keywords - Domaini

Coiled coil, Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410JE1U. Eukaryota.
ENOG41115GP. LUCA.
HOVERGENiHBG004902.
InParanoidiQ811A2.
KOiK06731.
OMAiECRNTTH.
OrthoDBiEOG7D59R5.
PhylomeDBiQ811A2.

Family and domain databases

InterProiIPR024886. BST2.
[Graphical view]
PANTHERiPTHR15190. PTHR15190. 1 hit.
PfamiPF16716. BST2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q811A2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAPSFYHYLP VAMDERWEPK GWSIRRWWLV AAILVVLIGV VLVCLIVYFA
60 70 80 90 100
NAAHSEACKN GLRLQDECRN TTHLLKHQLT RAQDSLLQTE MQANSCNQTV
110 120 130 140 150
MDLRDSLKKK VSQTQEQQAR IKELENKIER LNQELENLRT QKEISTTVQV
160 170
NSGGSVVVSS LLVLVAVLFL HF
Length:172
Mass (Da):19,674
Last modified:June 1, 2003 - v1
Checksum:i5B1018EBBDD0FB19
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ538349 mRNA. Translation: CAD61869.1.
RefSeqiNP_937767.1. NM_198134.2.
UniGeneiRn.234068.

Genome annotation databases

GeneIDi378947.
KEGGirno:378947.
UCSCiRGD:727849. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ538349 mRNA. Translation: CAD61869.1.
RefSeqiNP_937767.1. NM_198134.2.
UniGeneiRn.234068.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi266956. 1 interaction.
STRINGi10116.ENSRNOP00000031515.

PTM databases

iPTMnetiQ811A2.
PhosphoSiteiQ811A2.

Proteomic databases

PaxDbiQ811A2.
PRIDEiQ811A2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi378947.
KEGGirno:378947.
UCSCiRGD:727849. rat.

Organism-specific databases

CTDi684.
RGDi727849. Bst2.

Phylogenomic databases

eggNOGiENOG410JE1U. Eukaryota.
ENOG41115GP. LUCA.
HOVERGENiHBG004902.
InParanoidiQ811A2.
KOiK06731.
OMAiECRNTTH.
OrthoDBiEOG7D59R5.
PhylomeDBiQ811A2.

Miscellaneous databases

PROiQ811A2.

Family and domain databases

InterProiIPR024886. BST2.
[Graphical view]
PANTHERiPTHR15190. PTHR15190. 1 hit.
PfamiPF16716. BST2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Bst-2/HM1.24 is a raft-associated apical membrane protein with an unusual topology."
    Kupzig S., Korolchuk V., Rollason R., Sugden A., Wilde A., Banting G.
    Traffic 4:694-709(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, TOPOLOGY, GPI-ANCHOR, GLYCOSYLATION, SUBCELLULAR LOCATION.
    Strain: Wistar.
    Tissue: Liver.
  2. "A CD317/tetherin-RICH2 complex plays a critical role in the organization of the subapical actin cytoskeleton in polarized epithelial cells."
    Rollason R., Korolchuk V., Hamilton C., Jepson M., Banting G.
    J. Cell Biol. 184:721-736(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH ARHGAP44.
  3. "Endogenous CD317/Tetherin limits replication of HIV-1 and murine leukemia virus in rodent cells and is resistant to antagonists from primate viruses."
    Goffinet C., Schmidt S., Kern C., Oberbremer L., Keppler O.T.
    J. Virol. 84:11374-11384(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiBST2_RAT
AccessioniPrimary (citable) accession number: Q811A2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 17, 2006
Last sequence update: June 1, 2003
Last modified: July 6, 2016
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.