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Protein

Neurofascin

Gene

Nfasc

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Cell adhesion, ankyrin-binding protein which may be involved in neurite extension, axonal guidance, synaptogenesis, myelination and neuron-glial cell interactions.By similarity

GO - Molecular functioni

  • protein binding involved in heterotypic cell-cell adhesion Source: MGI

GO - Biological processi

  • axon guidance Source: MGI
  • clustering of voltage-gated sodium channels Source: MGI
  • heterotypic cell-cell adhesion Source: GOC
  • paranodal junction assembly Source: MGI
  • peripheral nervous system development Source: InterPro
  • protein localization to juxtaparanode region of axon Source: MGI
  • protein localization to paranode region of axon Source: MGI
  • synapse organization Source: MGI
  • transmission of nerve impulse Source: MGI
Complete GO annotation...

Keywords - Biological processi

Cell adhesion

Enzyme and pathway databases

ReactomeiREACT_318189. Neurofascin interactions.
REACT_318927. Interaction between L1 and Ankyrins.

Names & Taxonomyi

Protein namesi
Recommended name:
Neurofascin
Gene namesi
Name:Nfasc
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:104753. Nfasc.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini25 – 11101086ExtracellularSequence AnalysisAdd
BLAST
Transmembranei1111 – 113121HelicalSequence AnalysisAdd
BLAST
Topological domaini1132 – 1240109CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  • axon Source: MGI
  • extracellular exosome Source: MGI
  • focal adhesion Source: MGI
  • integral component of membrane Source: UniProtKB-KW
  • myelin sheath Source: UniProtKB
  • paranodal junction Source: MGI
  • paranode region of axon Source: BHF-UCL
  • plasma membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2424Sequence AnalysisAdd
BLAST
Chaini25 – 12401216NeurofascinPRO_0000015050Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi63 ↔ 118PROSITE-ProRule annotation
Disulfide bondi162 ↔ 213PROSITE-ProRule annotation
Disulfide bondi268 ↔ 316PROSITE-ProRule annotation
Glycosylationi305 – 3051N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi358 ↔ 408PROSITE-ProRule annotation
Glycosylationi409 – 4091N-linked (GlcNAc...)Sequence Analysis
Glycosylationi446 – 4461N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi452 ↔ 501PROSITE-ProRule annotation
Modified residuei481 – 4811PhosphotyrosineBy similarity
Glycosylationi483 – 4831N-linked (GlcNAc...)Sequence Analysis
Modified residuei485 – 4851PhosphoserineBy similarity
Disulfide bondi543 ↔ 592PROSITE-ProRule annotation
Glycosylationi752 – 7521N-linked (GlcNAc...)Sequence Analysis
Glycosylationi778 – 7781N-linked (GlcNAc...)Sequence Analysis
Glycosylationi866 – 8661N-linked (GlcNAc...)Sequence Analysis
Glycosylationi881 – 8811N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiQ810U3.
PaxDbiQ810U3.
PRIDEiQ810U3.

PTM databases

PhosphoSiteiQ810U3.

Expressioni

Gene expression databases

BgeeiQ810U3.
CleanExiMM_NFASC.
ExpressionAtlasiQ810U3. baseline and differential.
GenevisibleiQ810U3. MM.

Interactioni

Subunit structurei

Horseshoe-shaped homodimer. Probable constituent of a neurofascin/NRCAM/ankyrin-G complex. Associates with the sodium channel beta-1 (SCN1B) and beta-3 (SCN3B) subunits. Interacts with GLDN/gliomedin (By similarity). Interacts with MYOC.By similarity1 Publication

Protein-protein interaction databases

IntActiQ810U3. 3 interactions.
STRINGi10090.ENSMUSP00000092148.

Structurei

3D structure databases

ProteinModelPortaliQ810U3.
SMRiQ810U3. Positions 37-426.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini41 – 13797Ig-like C2-type 1Add
BLAST
Domaini143 – 23088Ig-like C2-type 2Add
BLAST
Domaini244 – 33289Ig-like C2-type 3Add
BLAST
Domaini337 – 42488Ig-like C2-type 4Add
BLAST
Domaini430 – 51788Ig-like C2-type 5Add
BLAST
Domaini521 – 60383Ig-like C2-type 6Add
BLAST
Domaini630 – 72596Fibronectin type-III 1PROSITE-ProRule annotationAdd
BLAST
Domaini727 – 82397Fibronectin type-III 2PROSITE-ProRule annotationAdd
BLAST
Domaini827 – 92397Fibronectin type-III 3PROSITE-ProRule annotationAdd
BLAST
Domaini1007 – 109993Fibronectin type-III 4PROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi924 – 100683Thr-richAdd
BLAST

Domaini

Homophilic adhesion is primarily mediated by the interaction of the second Ig-like domains.By similarity

Sequence similaritiesi

Contains 4 fibronectin type-III domains.PROSITE-ProRule annotation

Keywords - Domaini

Immunoglobulin domain, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG293951.
GeneTreeiENSGT00760000118840.
HOGENOMiHOG000231380.
HOVERGENiHBG000144.
InParanoidiQ810U3.
KOiK06757.
OrthoDBiEOG7R56RM.
PhylomeDBiQ810U3.
TreeFamiTF351098.

Family and domain databases

Gene3Di2.60.40.10. 10 hits.
InterProiIPR003961. FN3_dom.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR013098. Ig_I-set.
IPR003599. Ig_sub.
IPR003598. Ig_sub2.
IPR026966. Neurofascin/L1/NrCAM_C.
IPR026965. NFASC.
[Graphical view]
PANTHERiPTHR10489:SF41. PTHR10489:SF41. 1 hit.
PfamiPF13882. Bravo_FIGEY. 1 hit.
PF00041. fn3. 4 hits.
PF07679. I-set. 5 hits.
[Graphical view]
SMARTiSM00060. FN3. 4 hits.
SM00409. IG. 2 hits.
SM00408. IGc2. 4 hits.
[Graphical view]
SUPFAMiSSF49265. SSF49265. 3 hits.
PROSITEiPS50853. FN3. 4 hits.
PS50835. IG_LIKE. 6 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q810U3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MARQQAPPWV HIALILFLLS LGGAIEIPMD PSIQNELTQP PTITKQSVKD
60 70 80 90 100
HIVDPRDNIL IECEAKGNPA PSFHWTRNSR FFNIAKDPRV SMRRRSGTLV
110 120 130 140 150
IDFRSGGRPE EYEGEYQCFA RNKFGTALSN RIRLQVSKSP LWPKENLDPV
160 170 180 190 200
VVQEGAPLTL QCNPPPGLPS PVIFWMSSSM EPITQDKRVS QGHNGDLYFS
210 220 230 240 250
NVMLQDMQTD YSCNARFHFT HTIQQKNPFT LKVLTTRGVA ERTPSFMYPQ
260 270 280 290 300
GTSSSQMVLR GMDLLLECIA SGVPTPDIAW YKKGGDLPSN KAKFENFNKA
310 320 330 340 350
LRITNVSEED SGEYFCLASN KMGSIRHTIS VRVKAAPYWL DEPKNLILAP
360 370 380 390 400
GEDGRLVCRA NGNPKPTVQW MVNGEPLQSA PPNPNREVAG DTIIFRDTQI
410 420 430 440 450
SSRAVYQCNT SNEHGYLLAN AFVSVLDVPP RMLSARNQLI RVILYNRTRL
460 470 480 490 500
DCPFFGSPIP TLRWFKNGQG SNLDGGNYHV YENGSLEIKM IRKEDQGIYT
510 520 530 540 550
CVATNILGKA ENQVRLEVKD PTRIYRMPED QVAKRGTTVQ LECRVKHDPS
560 570 580 590 600
LKLTVSWLKD DEPLYIGNRM KKEDDSLTIF GVAERDQGSY TCMASTELDQ
610 620 630 640 650
DLAKAYLTVL ADQATPTNRL AALPKGRPDR PRDLELTDLA ERSVRLTWIP
660 670 680 690 700
GDDNNSPITD YVVQFEEDQF QPGVWHDHSR FPGSVNSAVL HLSPYVNYQF
710 720 730 740 750
RVIAVNEVGS SHPSLPSERY RTSGAPPESN PSDVKGEGTR KNNMEITWTP
760 770 780 790 800
MNATSAFGPN LRYIVKWRRR ETRETWNNVT VWGSRYVVGQ TPVYVPYEIR
810 820 830 840 850
VQAENDFGKG PEPDTIIGYS GEDLPSAPRR FRVRQPNLET INLEWDHPEH
860 870 880 890 900
PNGILIGYIL RYVPFNGTKL GKQMVENFSP NQTKFSVQRA DPVSRYRFSL
910 920 930 940 950
SARTQVGSGE AATEESPAPP NEATPTAAPP TLPPTTVGTT GLVSSTDATA
960 970 980 990 1000
LAATSEATTV PIIPTVVPTT VATTIATTTT TTAATTTTTT TESPPTTTAG
1010 1020 1030 1040 1050
TKIHETAPDE QSIWNVTVLP NSKWANITWK HNFRPGTDFV VEYIDSNHTK
1060 1070 1080 1090 1100
KTVPVKAQAQ PIQLTDLFPG MTYTLRVYSR DNEGISSTVI TFMTSTAYTN
1110 1120 1130 1140 1150
NQADIATQGW FIGLMCAIAL LVLILLIVCF IKRSRGGKYP VREKKDVPLG
1160 1170 1180 1190 1200
PEDPKEEDGS FDYSDEDNKP LQGSQTSLDG TIKQQESDDS LVDYGEGGEG
1210 1220 1230 1240
QFNEDGSFIG QYTVKKDKEE TEGNESSEAT SPVNAIYSLA
Length:1,240
Mass (Da):137,975
Last modified:June 1, 2003 - v1
Checksum:i6DE8935B5B02E965
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ543322 mRNA. Translation: CAD65849.1.
CCDSiCCDS35707.1.
RefSeqiNP_874385.1. NM_182716.4.
UniGeneiMm.326702.

Genome annotation databases

EnsembliENSMUST00000094569; ENSMUSP00000092148; ENSMUSG00000026442.
GeneIDi269116.
KEGGimmu:269116.
UCSCiuc007cpe.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ543322 mRNA. Translation: CAD65849.1.
CCDSiCCDS35707.1.
RefSeqiNP_874385.1. NM_182716.4.
UniGeneiMm.326702.

3D structure databases

ProteinModelPortaliQ810U3.
SMRiQ810U3. Positions 37-426.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ810U3. 3 interactions.
STRINGi10090.ENSMUSP00000092148.

PTM databases

PhosphoSiteiQ810U3.

Proteomic databases

MaxQBiQ810U3.
PaxDbiQ810U3.
PRIDEiQ810U3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000094569; ENSMUSP00000092148; ENSMUSG00000026442.
GeneIDi269116.
KEGGimmu:269116.
UCSCiuc007cpe.2. mouse.

Organism-specific databases

CTDi23114.
MGIiMGI:104753. Nfasc.

Phylogenomic databases

eggNOGiNOG293951.
GeneTreeiENSGT00760000118840.
HOGENOMiHOG000231380.
HOVERGENiHBG000144.
InParanoidiQ810U3.
KOiK06757.
OrthoDBiEOG7R56RM.
PhylomeDBiQ810U3.
TreeFamiTF351098.

Enzyme and pathway databases

ReactomeiREACT_318189. Neurofascin interactions.
REACT_318927. Interaction between L1 and Ankyrins.

Miscellaneous databases

ChiTaRSiNfasc. mouse.
NextBioi392698.
PROiQ810U3.
SOURCEiSearch...

Gene expression databases

BgeeiQ810U3.
CleanExiMM_NFASC.
ExpressionAtlasiQ810U3. baseline and differential.
GenevisibleiQ810U3. MM.

Family and domain databases

Gene3Di2.60.40.10. 10 hits.
InterProiIPR003961. FN3_dom.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR013098. Ig_I-set.
IPR003599. Ig_sub.
IPR003598. Ig_sub2.
IPR026966. Neurofascin/L1/NrCAM_C.
IPR026965. NFASC.
[Graphical view]
PANTHERiPTHR10489:SF41. PTHR10489:SF41. 1 hit.
PfamiPF13882. Bravo_FIGEY. 1 hit.
PF00041. fn3. 4 hits.
PF07679. I-set. 5 hits.
[Graphical view]
SMARTiSM00060. FN3. 4 hits.
SM00409. IG. 2 hits.
SM00408. IGc2. 4 hits.
[Graphical view]
SUPFAMiSSF49265. SSF49265. 3 hits.
PROSITEiPS50853. FN3. 4 hits.
PS50835. IG_LIKE. 6 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Expression patterns of L1-family cell recognition molecules L1, CHL1, NrCAM, and neurofascin in the mouse brain."
    Dirks P., Montag-Sallaz M., Montag D.
    Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Swiss Webster.
    Tissue: Brain.
  2. Lubec G., Kang S.U.
    Submitted (APR-2007) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 96-104; 243-260; 335-355; 387-396; 493-509; 573-585; 633-642; 681-719; 810-829 AND 1057-1076, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: C57BL/6.
    Tissue: Brain.
  3. "Myocilin mediates myelination in the peripheral nervous system through ErbB2/3 signaling."
    Kwon H.S., Johnson T.V., Joe M.K., Abu-Asab M., Zhang J., Chan C.C., Tomarev S.I.
    J. Biol. Chem. 288:26357-26371(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH MYOC.

Entry informationi

Entry nameiNFASC_MOUSE
AccessioniPrimary (citable) accession number: Q810U3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 14, 2003
Last sequence update: June 1, 2003
Last modified: June 24, 2015
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.