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Q810U3 (NFASC_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 101. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Neurofascin
Gene names
Name:Nfasc
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length1240 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Cell adhesion, ankyrin-binding protein which may be involved in neurite extension, axonal guidance, synaptogenesis, myelination and neuron-glial cell interactions By similarity.

Subunit structure

Horseshoe-shaped homodimer. Probable constituent of a neurofascin/NRCAM/ankyrin-G complex. Associates with the sodium channel beta-1 (SCN1B) and beta-3 (SCN3B) subunits. Interacts with GLDN/gliomedin By similarity. Interacts with MYOC. Ref.3

Subcellular location

Cell membrane; Single-pass type I membrane protein.

Domain

Homophilic adhesion is primarily mediated by the interaction of the second Ig-like domains By similarity.

Sequence similarities

Belongs to the immunoglobulin superfamily. L1/neurofascin/NgCAM family.

Contains 4 fibronectin type-III domains.

Contains 6 Ig-like C2-type (immunoglobulin-like) domains.

Ontologies

Keywords
   Biological processCell adhesion
   Cellular componentCell membrane
Membrane
   DomainImmunoglobulin domain
Repeat
Signal
Transmembrane
Transmembrane helix
   PTMDisulfide bond
Glycoprotein
Phosphoprotein
   Technical termComplete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processaxon guidance

Inferred from direct assay PubMed 15479642. Source: MGI

clustering of voltage-gated sodium channels

Inferred from mutant phenotype PubMed 20188654. Source: MGI

heterotypic cell-cell adhesion

Inferred from genetic interaction PubMed 20188654. Source: GOC

paranodal junction assembly

Inferred from mutant phenotype PubMed 16337912PubMed 20371806. Source: MGI

peripheral nervous system development

Inferred from electronic annotation. Source: InterPro

protein localization to juxtaparanode region of axon

Inferred from mutant phenotype PubMed 20371806. Source: MGI

protein localization to paranode region of axon

Inferred from mutant phenotype PubMed 20371806. Source: MGI

synapse organization

Inferred from mutant phenotype PubMed 15479642. Source: MGI

transmission of nerve impulse

Inferred from mutant phenotype PubMed 16337912. Source: MGI

   Cellular_componentaxon

Inferred from direct assay PubMed 15479642PubMed 16701205. Source: MGI

integral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

paranodal junction

Inferred from direct assay PubMed 18048693. Source: MGI

paranode region of axon

Inferred from direct assay PubMed 12975355. Source: BHF-UCL

plasma membrane

Inferred from direct assay PubMed 15479642. Source: MGI

   Molecular_functionprotein binding involved in heterotypic cell-cell adhesion

Inferred from genetic interaction PubMed 20188654. Source: MGI

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2424 Potential
Chain25 – 12401216Neurofascin
PRO_0000015050

Regions

Topological domain25 – 11101086Extracellular Potential
Transmembrane1111 – 113121Helical; Potential
Topological domain1132 – 1240109Cytoplasmic Potential
Domain41 – 13797Ig-like C2-type 1
Domain143 – 23088Ig-like C2-type 2
Domain244 – 33289Ig-like C2-type 3
Domain337 – 42488Ig-like C2-type 4
Domain430 – 51788Ig-like C2-type 5
Domain521 – 60383Ig-like C2-type 6
Domain630 – 72596Fibronectin type-III 1
Domain727 – 82397Fibronectin type-III 2
Domain827 – 92397Fibronectin type-III 3
Domain1007 – 109993Fibronectin type-III 4
Compositional bias924 – 100683Thr-rich

Amino acid modifications

Modified residue4811Phosphotyrosine By similarity
Modified residue4851Phosphoserine By similarity
Glycosylation3051N-linked (GlcNAc...) Potential
Glycosylation4091N-linked (GlcNAc...) Potential
Glycosylation4461N-linked (GlcNAc...) Potential
Glycosylation4831N-linked (GlcNAc...) Potential
Glycosylation7521N-linked (GlcNAc...) Potential
Glycosylation7781N-linked (GlcNAc...) Potential
Glycosylation8661N-linked (GlcNAc...) Potential
Glycosylation8811N-linked (GlcNAc...) Potential
Disulfide bond63 ↔ 118 By similarity
Disulfide bond162 ↔ 213 By similarity
Disulfide bond268 ↔ 316 By similarity
Disulfide bond358 ↔ 408 By similarity
Disulfide bond452 ↔ 501 Potential
Disulfide bond543 ↔ 592 Potential

Sequences

Sequence LengthMass (Da)Tools
Q810U3 [UniParc].

Last modified June 1, 2003. Version 1.
Checksum: 6DE8935B5B02E965

FASTA1,240137,975
        10         20         30         40         50         60 
MARQQAPPWV HIALILFLLS LGGAIEIPMD PSIQNELTQP PTITKQSVKD HIVDPRDNIL 

        70         80         90        100        110        120 
IECEAKGNPA PSFHWTRNSR FFNIAKDPRV SMRRRSGTLV IDFRSGGRPE EYEGEYQCFA 

       130        140        150        160        170        180 
RNKFGTALSN RIRLQVSKSP LWPKENLDPV VVQEGAPLTL QCNPPPGLPS PVIFWMSSSM 

       190        200        210        220        230        240 
EPITQDKRVS QGHNGDLYFS NVMLQDMQTD YSCNARFHFT HTIQQKNPFT LKVLTTRGVA 

       250        260        270        280        290        300 
ERTPSFMYPQ GTSSSQMVLR GMDLLLECIA SGVPTPDIAW YKKGGDLPSN KAKFENFNKA 

       310        320        330        340        350        360 
LRITNVSEED SGEYFCLASN KMGSIRHTIS VRVKAAPYWL DEPKNLILAP GEDGRLVCRA 

       370        380        390        400        410        420 
NGNPKPTVQW MVNGEPLQSA PPNPNREVAG DTIIFRDTQI SSRAVYQCNT SNEHGYLLAN 

       430        440        450        460        470        480 
AFVSVLDVPP RMLSARNQLI RVILYNRTRL DCPFFGSPIP TLRWFKNGQG SNLDGGNYHV 

       490        500        510        520        530        540 
YENGSLEIKM IRKEDQGIYT CVATNILGKA ENQVRLEVKD PTRIYRMPED QVAKRGTTVQ 

       550        560        570        580        590        600 
LECRVKHDPS LKLTVSWLKD DEPLYIGNRM KKEDDSLTIF GVAERDQGSY TCMASTELDQ 

       610        620        630        640        650        660 
DLAKAYLTVL ADQATPTNRL AALPKGRPDR PRDLELTDLA ERSVRLTWIP GDDNNSPITD 

       670        680        690        700        710        720 
YVVQFEEDQF QPGVWHDHSR FPGSVNSAVL HLSPYVNYQF RVIAVNEVGS SHPSLPSERY 

       730        740        750        760        770        780 
RTSGAPPESN PSDVKGEGTR KNNMEITWTP MNATSAFGPN LRYIVKWRRR ETRETWNNVT 

       790        800        810        820        830        840 
VWGSRYVVGQ TPVYVPYEIR VQAENDFGKG PEPDTIIGYS GEDLPSAPRR FRVRQPNLET 

       850        860        870        880        890        900 
INLEWDHPEH PNGILIGYIL RYVPFNGTKL GKQMVENFSP NQTKFSVQRA DPVSRYRFSL 

       910        920        930        940        950        960 
SARTQVGSGE AATEESPAPP NEATPTAAPP TLPPTTVGTT GLVSSTDATA LAATSEATTV 

       970        980        990       1000       1010       1020 
PIIPTVVPTT VATTIATTTT TTAATTTTTT TESPPTTTAG TKIHETAPDE QSIWNVTVLP 

      1030       1040       1050       1060       1070       1080 
NSKWANITWK HNFRPGTDFV VEYIDSNHTK KTVPVKAQAQ PIQLTDLFPG MTYTLRVYSR 

      1090       1100       1110       1120       1130       1140 
DNEGISSTVI TFMTSTAYTN NQADIATQGW FIGLMCAIAL LVLILLIVCF IKRSRGGKYP 

      1150       1160       1170       1180       1190       1200 
VREKKDVPLG PEDPKEEDGS FDYSDEDNKP LQGSQTSLDG TIKQQESDDS LVDYGEGGEG 

      1210       1220       1230       1240 
QFNEDGSFIG QYTVKKDKEE TEGNESSEAT SPVNAIYSLA 

« Hide

References

[1]"Expression patterns of L1-family cell recognition molecules L1, CHL1, NrCAM, and neurofascin in the mouse brain."
Dirks P., Montag-Sallaz M., Montag D.
Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: Swiss Webster.
Tissue: Brain.
[2]Lubec G., Kang S.U.
Submitted (APR-2007) to UniProtKB
Cited for: PROTEIN SEQUENCE OF 96-104; 243-260; 335-355; 387-396; 493-509; 573-585; 633-642; 681-719; 810-829 AND 1057-1076, IDENTIFICATION BY MASS SPECTROMETRY.
Strain: C57BL/6.
Tissue: Brain.
[3]"Myocilin mediates myelination in the peripheral nervous system through ErbB2/3 signaling."
Kwon H.S., Johnson T.V., Joe M.K., Abu-Asab M., Zhang J., Chan C.C., Tomarev S.I.
J. Biol. Chem. 288:26357-26371(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH MYOC.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AJ543322 mRNA. Translation: CAD65849.1.
RefSeqNP_874385.1. NM_182716.4.
UniGeneMm.326702.

3D structure databases

ProteinModelPortalQ810U3.
SMRQ810U3. Positions 37-915.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ810U3. 3 interactions.

PTM databases

PhosphoSiteQ810U3.

Proteomic databases

PaxDbQ810U3.
PRIDEQ810U3.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000094569; ENSMUSP00000092148; ENSMUSG00000026442.
GeneID269116.
KEGGmmu:269116.
UCSCuc007cpe.2. mouse.

Organism-specific databases

CTD23114.
MGIMGI:104753. Nfasc.

Phylogenomic databases

eggNOGNOG293951.
GeneTreeENSGT00690000101868.
HOGENOMHOG000231380.
HOVERGENHBG000144.
KOK06757.
OrthoDBEOG7R56RM.
PhylomeDBQ810U3.
TreeFamTF351098.

Enzyme and pathway databases

ReactomeREACT_188576. Developmental Biology.

Gene expression databases

BgeeQ810U3.
CleanExMM_NFASC.
GenevestigatorQ810U3.

Family and domain databases

Gene3D2.60.40.10. 10 hits.
InterProIPR003961. Fibronectin_type3.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR013098. Ig_I-set.
IPR003599. Ig_sub.
IPR003598. Ig_sub2.
IPR026966. Neurofascin/L1/NrCAM_C.
IPR026965. NFASC.
[Graphical view]
PANTHERPTHR10489:SF41. PTHR10489:SF41. 1 hit.
PfamPF13882. Bravo_FIGEY. 1 hit.
PF00041. fn3. 4 hits.
PF07679. I-set. 5 hits.
[Graphical view]
SMARTSM00060. FN3. 4 hits.
SM00409. IG. 2 hits.
SM00408. IGc2. 4 hits.
[Graphical view]
SUPFAMSSF49265. SSF49265. 3 hits.
PROSITEPS50853. FN3. 4 hits.
PS50835. IG_LIKE. 6 hits.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSNFASC. mouse.
NextBio392698.
PROQ810U3.
SOURCESearch...

Entry information

Entry nameNFASC_MOUSE
AccessionPrimary (citable) accession number: Q810U3
Entry history
Integrated into UniProtKB/Swiss-Prot: November 14, 2003
Last sequence update: June 1, 2003
Last modified: April 16, 2014
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot