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Protein

E3 ubiquitin-protein ligase TRIM50

Gene

Trim50

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

E3 ubiquitin-protein ligase.By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri16 – 5742RING-typePROSITE-ProRule annotationAdd
BLAST
Zinc fingeri84 – 12542B box-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

  • regulation of establishment of protein localization Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Ubl conjugation pathway

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BRENDAi6.3.2.19. 3474.
ReactomeiR-MMU-983168. Antigen processing: Ubiquitination & Proteasome degradation.

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase TRIM50 (EC:6.3.2.-)
Alternative name(s):
Tripartite motif-containing protein 50
Gene namesi
Name:Trim50
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:2664992. Trim50.

Subcellular locationi

GO - Cellular componenti

  • aggresome Source: MGI
  • cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 483483E3 ubiquitin-protein ligase TRIM50PRO_0000056275Add
BLAST

Post-translational modificationi

Auto-ubiquitinated.By similarity

Keywords - PTMi

Ubl conjugation

Proteomic databases

PaxDbiQ810I2.
PRIDEiQ810I2.

PTM databases

PhosphoSiteiQ810I2.

Expressioni

Tissue specificityi

Expressed in the stomach.1 Publication

Gene expression databases

CleanExiMM_TRIM50.
ExpressionAtlasiQ810I2. baseline and differential.

Interactioni

Subunit structurei

Can form dimers and trimers. Interacts with several E2 ubiquitin-conjugating enzymes, including UBE2L6, UBE2E1, UBE2E3. No interaction with UBE2H (By similarity).By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000066662.

Structurei

3D structure databases

ProteinModelPortaliQ810I2.
SMRiQ810I2. Positions 10-56, 89-230, 282-469.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini275 – 474200B30.2/SPRYPROSITE-ProRule annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili127 – 16943Sequence analysisAdd
BLAST
Coiled coili203 – 23634Sequence analysisAdd
BLAST

Sequence similaritiesi

Belongs to the TRIM/RBCC family.Curated
Contains 1 B box-type zinc finger.PROSITE-ProRule annotation
Contains 1 B30.2/SPRY domain.PROSITE-ProRule annotation
Contains 1 RING-type zinc finger.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri16 – 5742RING-typePROSITE-ProRule annotationAdd
BLAST
Zinc fingeri84 – 12542B box-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Coiled coil, Zinc-finger

Phylogenomic databases

eggNOGiENOG410ITFB. Eukaryota.
ENOG410XWXM. LUCA.
GeneTreeiENSGT00760000118838.
HOGENOMiHOG000234133.
HOVERGENiHBG106596.
InParanoidiQ810I2.
KOiK12024.
PhylomeDBiQ810I2.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
4.10.45.10. 1 hit.
InterProiIPR001870. B30.2/SPRY.
IPR003879. Butyrophylin.
IPR013320. ConA-like_dom.
IPR006574. PRY.
IPR003877. SPRY_dom.
IPR027370. Znf-RING_LisH.
IPR000315. Znf_B-box.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR017907. Znf_RING_CS.
[Graphical view]
PfamiPF13765. PRY. 1 hit.
PF00622. SPRY. 1 hit.
PF00643. zf-B_box. 1 hit.
PF13445. zf-RING_UBOX. 1 hit.
[Graphical view]
PRINTSiPR01407. BUTYPHLNCDUF.
SMARTiSM00336. BBOX. 1 hit.
SM00589. PRY. 1 hit.
SM00184. RING. 1 hit.
SM00449. SPRY. 1 hit.
[Graphical view]
SUPFAMiSSF49899. SSF49899. 1 hit.
PROSITEiPS50188. B302_SPRY. 1 hit.
PS50119. ZF_BBOX. 1 hit.
PS00518. ZF_RING_1. 1 hit.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q810I2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAWRLTVPEL QDQLQCPICL EVFKEPLMLQ CGHSYCKDCL DNLSQHLDSE
60 70 80 90 100
LCCPVCRQSV DCSSSPPNVS LARVIDALRL PGDIEPTVCV HHRNPLSLFC
110 120 130 140 150
EKDQEFICGL CGLLGSHQHH RVTPVSTVYS RMKEELAGRI SELKEEHRNV
160 170 180 190 200
EEHIGKLVNN RTRIINESDV FSWVIRREFQ ELHHLVDEEK ARCLEGLEGH
210 220 230 240 250
TRGLVASLDM QLEQAQGTQE RLAQAEQVLE QFGNESHHEF IRFHSVASRA
260 270 280 290 300
EVQQARPLEG VFSPISFKPA LHQADIKLTV WKRLFRKVLP APASLKLDPA
310 320 330 340 350
TAHPLLELSK GNTVVHCGLL AQRRASQPER FDYSTCVLAS KGFSWGRHYW
360 370 380 390 400
EVVVGSKSDW RLGVIKGTAS RKGKLNKSPE HGVWLIGLKE GRVYEAFGCP
410 420 430 440 450
RLPLPVAGHP HRIGVYLHYE QGELTFFDAD RPDDLRTLYT FQADFQGKLY
460 470 480
PILDTCWHER GSNSLPMVLP PPSGPGHFTL GQV
Length:483
Mass (Da):54,607
Last modified:June 1, 2003 - v1
Checksum:iDA3A7234C1A8145B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY081947 mRNA. Translation: AAL91070.1.
CCDSiCCDS19739.1.
RefSeqiNP_839971.1. NM_178240.2.
XP_006504475.1. XM_006504412.2.
UniGeneiMm.261834.

Genome annotation databases

EnsembliENSMUST00000065785; ENSMUSP00000066662; ENSMUSG00000053388.
GeneIDi215061.
KEGGimmu:215061.
UCSCiuc008zyf.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY081947 mRNA. Translation: AAL91070.1.
CCDSiCCDS19739.1.
RefSeqiNP_839971.1. NM_178240.2.
XP_006504475.1. XM_006504412.2.
UniGeneiMm.261834.

3D structure databases

ProteinModelPortaliQ810I2.
SMRiQ810I2. Positions 10-56, 89-230, 282-469.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000066662.

PTM databases

PhosphoSiteiQ810I2.

Proteomic databases

PaxDbiQ810I2.
PRIDEiQ810I2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000065785; ENSMUSP00000066662; ENSMUSG00000053388.
GeneIDi215061.
KEGGimmu:215061.
UCSCiuc008zyf.1. mouse.

Organism-specific databases

CTDi135892.
MGIiMGI:2664992. Trim50.

Phylogenomic databases

eggNOGiENOG410ITFB. Eukaryota.
ENOG410XWXM. LUCA.
GeneTreeiENSGT00760000118838.
HOGENOMiHOG000234133.
HOVERGENiHBG106596.
InParanoidiQ810I2.
KOiK12024.
PhylomeDBiQ810I2.

Enzyme and pathway databases

BRENDAi6.3.2.19. 3474.
ReactomeiR-MMU-983168. Antigen processing: Ubiquitination & Proteasome degradation.

Miscellaneous databases

NextBioi374596.
PROiQ810I2.
SOURCEiSearch...

Gene expression databases

CleanExiMM_TRIM50.
ExpressionAtlasiQ810I2. baseline and differential.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
4.10.45.10. 1 hit.
InterProiIPR001870. B30.2/SPRY.
IPR003879. Butyrophylin.
IPR013320. ConA-like_dom.
IPR006574. PRY.
IPR003877. SPRY_dom.
IPR027370. Znf-RING_LisH.
IPR000315. Znf_B-box.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR017907. Znf_RING_CS.
[Graphical view]
PfamiPF13765. PRY. 1 hit.
PF00622. SPRY. 1 hit.
PF00643. zf-B_box. 1 hit.
PF13445. zf-RING_UBOX. 1 hit.
[Graphical view]
PRINTSiPR01407. BUTYPHLNCDUF.
SMARTiSM00336. BBOX. 1 hit.
SM00589. PRY. 1 hit.
SM00184. RING. 1 hit.
SM00449. SPRY. 1 hit.
[Graphical view]
SUPFAMiSSF49899. SSF49899. 1 hit.
PROSITEiPS50188. B302_SPRY. 1 hit.
PS50119. ZF_BBOX. 1 hit.
PS00518. ZF_RING_1. 1 hit.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.

Entry informationi

Entry nameiTRI50_MOUSE
AccessioniPrimary (citable) accession number: Q810I2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 7, 2004
Last sequence update: June 1, 2003
Last modified: November 11, 2015
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.