Reviewed, UniProtKB/Swiss-Prot Q810B3 (BSH_MOUSE)

Names and origin

Protein names

Recommended name:
    Brain-specific homeobox protein homolog

Gene names

Name:

Bsx

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	232 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	DNA binding protein that function as transcriptional activator. Is essentiel for normal postnatal growth and nursing. Is an essential factor for neuronal neuropeptide Y and agouti-related peptide function and locomotory behavior in the control of energy balance. Ref.2 Ref.5 Ref.6
Subcellular location	Isoform 1: Nucleus. Isoform 2: Cytoplasm.
Tissue specificity	Expressed in brain. In brain, it is restricted to a few specific developing brain structures such as pineal gland, telencephalic septum, hypothalamic pre-mammillary body and arcuate nucleus. Ref.4
Developmental stage	Expressed in early embryonic stages of epiphysis development from E9.5 onwards. Ref.4
Sequence similarities	Belongs to the distal-less homeobox family. Contains 1 homeobox DNA-binding domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Cytoplasm Nucleus
Coding sequence diversity	Alternative splicing
Domain	Homeobox
Ligand	DNA-binding
Molecular function	Activator
Gene Ontology (GO)
Biological process	brain development Inferred from sequence or structural similarity. Source: UniProtKB eating behavior Ref.5 Inferred from mutant phenotype. Source: MGI locomotory behavior Ref.5 Inferred from mutant phenotype. Source: MGI mammary gland involution Ref.6 Inferred from mutant phenotype. Source: MGI positive regulation of transcription from RNA polymerase II promoter Ref.2 Ref.5 Inferred from direct assay. Source: MGI transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Ref.2 Inferred from direct assay. Source: MGI transcription factor complex Ref.5 Inferred from direct assay. Source: MGI
Molecular function	protein binding Ref.5 Inferred from physical interaction. Source: MGI sequence-specific DNA binding Ref.2 Ref.5 Inferred from direct assay. Source: MGI transcription factor activity Ref.2 Inferred from direct assay. Source: MGI
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q810B3-1)

Also known as: Bsxla;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q810B3-2)

Also known as: Bsxlb;

The sequence of this isoform differs from the canonical sequence as follows:
     88-131: GMPVPALFPH...LSGLEKRFEI → ALRPGEKVRN...NVVPEPADEA
     132-232: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 232	232	Brain-specific homeobox protein homolog		PRO_0000048844
Regions
	DNA binding	108 – 168	61	Homeobox
	Compositional bias	68 – 80	13	His-rich
Natural variations
	Alternative sequence	88 – 131	44	GMPVP…KRFEI → ALRPGEKVRNPALPVDARAC GAHCPQPLRDSGENVVPEPA DEA in isoform 2.		VSP_027570
	Alternative sequence	132 – 232	101	Missing in isoform 2.		VSP_027571
Experimental info
	Mutagenesis	109	1	R → G: Abolishes the nuclear localization; when associated with G-101. Ref.2
	Mutagenesis	111	1	R → G: Abolishes the nuclear localization; when associated with G-109. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (Bsxla) [UniParc]. Last modified June 1, 2003. Version 1. Checksum: FEE7C9F1574F5624	FASTA	232	25,862
	10 20 30 40 50 60 MNLNFTSPLH PASSQRPTSF FIEDILLHKP KPLREVAPDH FASSLASRVP LLDYGYPLMP 70 80 90 100 110 120 TPTLLTPHAH HPLHKGDHHH PYFLTTSGMP VPALFPHPQH AELPGKHCRR RKARTVFSDS 130 140 150 160 170 180 QLSGLEKRFE IQRYLSTPER VELATALSLS ETQVKTWFQN RRMKHKKQLR KSQDEPKAAD 190 200 210 220 230 GPESPEGSPR APEGAPADAR LSLPAGAFVL TEPEDEVDIG DEGELSSGPH VL
	Isoform 2 (Bsxlb). Checksum: 7CA6FD721CB2ED1D	FASTA	130	14,323
	10 20 30 40 50 60 MNLNFTSPLH PASSQRPTSF FIEDILLHKP KPLREVAPDH FASSLASRVP LLDYGYPLMP 70 80 90 100 110 120 TPTLLTPHAH HPLHKGDHHH PYFLTTSALR PGEKVRNPAL PVDARACGAH CPQPLRDSGE 130 NVVPEPADEA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of vertebrate homologs of the Drosophila brain-specific homeobox gene." Assimacopoulos S., Ragsdale C.W. Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: CD-1. Tissue: Hypothalamus and Pineal gland.
[2]	"Cloning and functional analysis of hypothalamic homeobox gene Bsx1a and its isoform, Bsx1b." Chu H.-Y., Ohtoshi A. Mol. Cell. Biol. 27:3743-3749(2007) [PubMed: 17353277] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), FUNCTION, MUTAGENESIS OF ARG-109 AND ARG-111, SUBCELLULAR LOCATION.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[4]	"Bsx, an evolutionary conserved brain specific homeobox gene expressed in the septum, epiphysis, mammillary bodies and arcuate nucleus." Cremona M., Colombo E., Andreazzoli M., Cossu G., Broccoli V. Gene Expr. Patterns 4:47-51(2004) [PubMed: 14678827] [Abstract] Cited for: TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
[5]	"A role for brain-specific homeobox factor bsx in the control of hyperphagia and locomotory behavior." Sakkou M., Wiedmer P., Anlag K., Hamm A., Seuntjens E., Ettwiller L., Tschoep M.H., Treier M. Cell Metab. 5:450-463(2007) [PubMed: 17550780] [Abstract] Cited for: FUNCTION.
[6]	"A brain-specific homeobox gene, bsx, is essential for proper postnatal growth and nursing." McArthur T., Ohtoshi A. Mol. Cell. Biol. 27:5120-5127(2007) [PubMed: 17485440] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Web resources

Protein Spotlight

Of fidgets and food - Issue 85 of August 2007

Cross-references

Sequence databases
EMBLGenBankDDBJ	AY233396 mRNA. Translation: AAO84023.1. BC104386 mRNA. Translation: AAI04387.1. BC104387 mRNA. Translation: AAI04388.1.
IPI	IPI00329841. IPI00857232.
RefSeq	NP_839976.1.
UniGene	Mm.261652
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q810B3.
Proteomic databases
PRIDE	Q810B3.
Genome annotation databases
Ensembl	ENSMUST00000067375; ENSMUSP00000068057; ENSMUSG00000054360; Mus musculus. [Genome view]
GeneID	244813.
KEGG	mmu:244813.
UCSC	uc009paa.1. mouse.
Organism-specific databases
CTD	244813.
MGI	MGI:2669849. Bsx.
Phylogenomic databases
HOGENOM	Q810B3.
HOVERGEN	Q810B3.
OMA	ILAPHPH
OrthoDB	EOG9QC3QS
Gene expression databases
ArrayExpress	Q810B3.
Bgee	Q810B3.
CleanEx	MM_BSX.
Genevestigator	Q810B3.
Family and domain databases
InterPro	IPR001356. Homeobox. IPR017970. Homeobox_CS. IPR020479. Homeobox_region. IPR009057. Homeodomain-like. IPR012287. Homeodomain-rel. [Graphical view]
Gene3D	G3DSA:1.10.10.60. Homeodomain-rel. 1 hit.
Pfam	PF00046. Homeobox. 1 hit. [Graphical view]
PRINTS	PR00024. HOMEOBOX.
SMART	SM00389. HOX. 1 hit. [Graphical view]
PROSITE	PS00027. HOMEOBOX_1. 1 hit. PS50071. HOMEOBOX_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	386432.
SOURCE	Search...

Entry information

Entry name

BSH_MOUSE

Accession

Primary (citable) accession number: Q810B3
Secondary accession number(s): Q3SXB1

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 31, 2004
Last sequence update:	June 1, 2003
Last modified:	November 24, 2009
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)