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Protein

Brain-specific homeobox protein homolog

Gene

Bsx

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

DNA binding protein that function as transcriptional activator. Is essentiel for normal postnatal growth and nursing. Is an essential factor for neuronal neuropeptide Y and agouti-related peptide function and locomotory behavior in the control of energy balance.3 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi108 – 16861HomeoboxPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. RNA polymerase II core promoter proximal region sequence-specific DNA binding Source: NTNU_SB
  2. RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription Source: NTNU_SB
  3. sequence-specific DNA binding Source: MGI
  4. sequence-specific DNA binding transcription factor activity Source: MGI

GO - Biological processi

  1. brain development Source: UniProtKB
  2. eating behavior Source: MGI
  3. locomotory behavior Source: MGI
  4. mammary gland involution Source: MGI
  5. positive regulation of transcription from RNA polymerase II promoter Source: NTNU_SB
  6. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Brain-specific homeobox protein homolog
Gene namesi
Name:BsxImported
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 9

Organism-specific databases

MGIiMGI:2669849. Bsx.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: MGI
  2. nucleus Source: MGI
  3. transcription factor complex Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi109 – 1091R → G: Abolishes the nuclear localization; when associated with G-101. 1 Publication
Mutagenesisi111 – 1111R → G: Abolishes the nuclear localization; when associated with G-109. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 232232Brain-specific homeobox protein homologPRO_0000048844Add
BLAST

Proteomic databases

PRIDEiQ810B3.

PTM databases

PhosphoSiteiQ810B3.

Expressioni

Tissue specificityi

Expressed in brain. In brain, it is restricted to a few specific developing brain structures such as pineal gland, telencephalic septum, hypothalamic pre-mammillary body and arcuate nucleus.1 Publication

Developmental stagei

Expressed in early embryonic stages of epiphysis development from E9.5 onwards.1 Publication

Gene expression databases

BgeeiQ810B3.
CleanExiMM_BSX.
GenevestigatoriQ810B3.

Interactioni

Protein-protein interaction databases

BioGridi232691. 2 interactions.

Structurei

3D structure databases

ProteinModelPortaliQ810B3.
SMRiQ810B3. Positions 104-167.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi68 – 8013His-richAdd
BLAST

Sequence similaritiesi

Belongs to the distal-less homeobox family.Sequence Analysis
Contains 1 homeobox DNA-binding domain.PROSITE-ProRule annotation

Keywords - Domaini

Homeobox

Phylogenomic databases

eggNOGiNOG242366.
GeneTreeiENSGT00780000121835.
HOGENOMiHOG000231924.
HOVERGENiHBG050738.
InParanoidiQ810B3.
OMAiLHKPEHH.
OrthoDBiEOG7QVM51.
PhylomeDBiQ810B3.
TreeFamiTF350735.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
InterProiIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR020479. Homeobox_metazoa.
IPR009057. Homeodomain-like.
[Graphical view]
PfamiPF00046. Homeobox. 1 hit.
[Graphical view]
PRINTSiPR00024. HOMEOBOX.
SMARTiSM00389. HOX. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
PROSITEiPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q810B3-1) [UniParc]FASTAAdd to basket

Also known as: Bsxla

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MNLNFTSPLH PASSQRPTSF FIEDILLHKP KPLREVAPDH FASSLASRVP
60 70 80 90 100
LLDYGYPLMP TPTLLTPHAH HPLHKGDHHH PYFLTTSGMP VPALFPHPQH
110 120 130 140 150
AELPGKHCRR RKARTVFSDS QLSGLEKRFE IQRYLSTPER VELATALSLS
160 170 180 190 200
ETQVKTWFQN RRMKHKKQLR KSQDEPKAAD GPESPEGSPR APEGAPADAR
210 220 230
LSLPAGAFVL TEPEDEVDIG DEGELSSGPH VL
Length:232
Mass (Da):25,862
Last modified:June 1, 2003 - v1
Checksum:iFEE7C9F1574F5624
GO
Isoform 2 (identifier: Q810B3-2) [UniParc]FASTAAdd to basket

Also known as: Bsxlb

The sequence of this isoform differs from the canonical sequence as follows:
     88-131: GMPVPALFPH...LSGLEKRFEI → ALRPGEKVRN...NVVPEPADEA
     132-232: Missing.

Show »
Length:130
Mass (Da):14,323
Checksum:i7CA6FD721CB2ED1D
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei88 – 13144GMPVP…KRFEI → ALRPGEKVRNPALPVDARAC GAHCPQPLRDSGENVVPEPA DEA in isoform 2. 1 PublicationVSP_027570Add
BLAST
Alternative sequencei132 – 232101Missing in isoform 2. 1 PublicationVSP_027571Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY233396 mRNA. Translation: AAO84023.1.
BC104386 mRNA. Translation: AAI04387.1.
BC104387 mRNA. Translation: AAI04388.1.
CCDSiCCDS23084.1. [Q810B3-1]
RefSeqiNP_839976.1. NM_178245.3. [Q810B3-1]
UniGeneiMm.261652.

Genome annotation databases

EnsembliENSMUST00000067375; ENSMUSP00000068057; ENSMUSG00000054360. [Q810B3-1]
GeneIDi244813.
KEGGimmu:244813.
UCSCiuc009paa.1. mouse. [Q810B3-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Web resourcesi

Protein Spotlight

Of fidgets and food - Issue 85 of August 2007

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY233396 mRNA. Translation: AAO84023.1.
BC104386 mRNA. Translation: AAI04387.1.
BC104387 mRNA. Translation: AAI04388.1.
CCDSiCCDS23084.1. [Q810B3-1]
RefSeqiNP_839976.1. NM_178245.3. [Q810B3-1]
UniGeneiMm.261652.

3D structure databases

ProteinModelPortaliQ810B3.
SMRiQ810B3. Positions 104-167.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi232691. 2 interactions.

PTM databases

PhosphoSiteiQ810B3.

Proteomic databases

PRIDEiQ810B3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000067375; ENSMUSP00000068057; ENSMUSG00000054360. [Q810B3-1]
GeneIDi244813.
KEGGimmu:244813.
UCSCiuc009paa.1. mouse. [Q810B3-1]

Organism-specific databases

CTDi390259.
MGIiMGI:2669849. Bsx.

Phylogenomic databases

eggNOGiNOG242366.
GeneTreeiENSGT00780000121835.
HOGENOMiHOG000231924.
HOVERGENiHBG050738.
InParanoidiQ810B3.
OMAiLHKPEHH.
OrthoDBiEOG7QVM51.
PhylomeDBiQ810B3.
TreeFamiTF350735.

Miscellaneous databases

NextBioi386432.
PROiQ810B3.
SOURCEiSearch...

Gene expression databases

BgeeiQ810B3.
CleanExiMM_BSX.
GenevestigatoriQ810B3.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
InterProiIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR020479. Homeobox_metazoa.
IPR009057. Homeodomain-like.
[Graphical view]
PfamiPF00046. Homeobox. 1 hit.
[Graphical view]
PRINTSiPR00024. HOMEOBOX.
SMARTiSM00389. HOX. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
PROSITEiPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Identification of vertebrate homologs of the Drosophila brain-specific homeobox gene."
    Assimacopoulos S., Ragsdale C.W.
    Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: CD-1Imported.
    Tissue: Hypothalamus1 Publication and Pineal gland1 Publication.
  2. "Cloning and functional analysis of hypothalamic homeobox gene Bsx1a and its isoform, Bsx1b."
    Chu H.-Y., Ohtoshi A.
    Mol. Cell. Biol. 27:3743-3749(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), FUNCTION, MUTAGENESIS OF ARG-109 AND ARG-111, SUBCELLULAR LOCATION.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
  4. "Bsx, an evolutionary conserved brain specific homeobox gene expressed in the septum, epiphysis, mammillary bodies and arcuate nucleus."
    Cremona M., Colombo E., Andreazzoli M., Cossu G., Broccoli V.
    Gene Expr. Patterns 4:47-51(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
  5. "A role for brain-specific homeobox factor bsx in the control of hyperphagia and locomotory behavior."
    Sakkou M., Wiedmer P., Anlag K., Hamm A., Seuntjens E., Ettwiller L., Tschoep M.H., Treier M.
    Cell Metab. 5:450-463(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  6. "A brain-specific homeobox gene, bsx, is essential for proper postnatal growth and nursing."
    McArthur T., Ohtoshi A.
    Mol. Cell. Biol. 27:5120-5127(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiBSH_MOUSE
AccessioniPrimary (citable) accession number: Q810B3
Secondary accession number(s): Q3SXB1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 31, 2004
Last sequence update: June 1, 2003
Last modified: February 4, 2015
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Protein Spotlight
    Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.