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Protein

Sperm-associated antigen 1

Gene

Spag1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

May play a role in the cytoplasmic assembly of the ciliary dynein arms (By similarity). May play a role in fertilization. Binds GTP and has GTPase activity (By similarity).By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi756 – 7638GTPSequence analysis

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Fertilization

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Sperm-associated antigen 1
Alternative name(s):
Infertility-related sperm protein Spag-1
TPR-containing protein involved in spermatogenesis
Short name:
TPIS
Gene namesi
Name:Spag1
Synonyms:Tpis
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 15

Organism-specific databases

MGIiMGI:1349387. Spag1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 901901Sperm-associated antigen 1PRO_0000106325Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei351 – 3511PhosphoserineCombined sources
Modified residuei359 – 3591PhosphoserineCombined sources
Modified residuei703 – 7031PhosphoserineBy similarity
Modified residuei739 – 7391PhosphoserineCombined sources
Modified residuei740 – 7401PhosphoserineCombined sources
Modified residuei766 – 7661PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ80ZX8.
MaxQBiQ80ZX8.
PaxDbiQ80ZX8.
PRIDEiQ80ZX8.

PTM databases

iPTMnetiQ80ZX8.
PhosphoSiteiQ80ZX8.

Expressioni

Tissue specificityi

Detected in cerebellum, tongue, esophagus, forestomach, sperm and testis.1 Publication

Developmental stagei

Expression is very low in embryonic epidermis at E13.5 and increases from E14.5 to E16.5. In young mice expression increases in the testis of 2 to 6 weeks old animals, and then remains stable.1 Publication

Gene expression databases

BgeeiQ80ZX8.
GenevisibleiQ80ZX8. MM.

Interactioni

Protein-protein interaction databases

BioGridi205078. 1 interaction.
STRINGi10090.ENSMUSP00000047335.

Structurei

3D structure databases

ProteinModelPortaliQ80ZX8.
SMRiQ80ZX8. Positions 143-328, 432-573, 604-737.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati213 – 24634TPR 1Add
BLAST
Repeati247 – 27933TPR 2Add
BLAST
Repeati280 – 31334TPR 3Add
BLAST
Repeati430 – 46435TPR 4Add
BLAST
Repeati472 – 50534TPR 5Add
BLAST
Repeati507 – 53933TPR 6Add
BLAST
Repeati606 – 63934TPR 7Add
BLAST
Repeati640 – 67334TPR 8Add
BLAST

Sequence similaritiesi

Contains 8 TPR repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, TPR repeat

Phylogenomic databases

eggNOGiKOG1124. Eukaryota.
COG0457. LUCA.
GeneTreeiENSGT00840000129761.
HOGENOMiHOG000293399.
HOVERGENiHBG079204.
InParanoidiQ80ZX8.
KOiK19870.
OMAiPLQAWHP.
OrthoDBiEOG75QR4W.
PhylomeDBiQ80ZX8.
TreeFamiTF106251.

Family and domain databases

Gene3Di1.25.40.10. 3 hits.
InterProiIPR025986. RPAP3-like_C.
IPR013026. TPR-contain_dom.
IPR011990. TPR-like_helical_dom.
IPR001440. TPR_1.
IPR019734. TPR_repeat.
[Graphical view]
PfamiPF13877. RPAP3_C. 1 hit.
PF00515. TPR_1. 2 hits.
PF13181. TPR_8. 1 hit.
[Graphical view]
SMARTiSM00028. TPR. 8 hits.
[Graphical view]
SUPFAMiSSF48452. SSF48452. 3 hits.
PROSITEiPS50005. TPR. 6 hits.
PS50293. TPR_REGION. 4 hits.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q80ZX8-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTAKAKDCPS LWGFGTTKTF KIPIEHLDFK YIENCSDVKH LEKILYVLRS
60 70 80 90 100
GEEGYYPELT EFCEKCLTNL APKSRALRKD KPAETASSFS AEEWEKIDSD
110 120 130 140 150
LKSWVSEIKR EENTCHFHDP ENHPGVEDPL PPVRGSTCCP HSGKETYSKS
160 170 180 190 200
KTAKKRIPRD YAEWDKFDVE KECSKIDEDY KEKTVINNKA HLSKIETKIE
210 220 230 240 250
TAGLTEKEKS FLANREKGKG NEAFYSGDYE EAVMYYTRSL SALPTAIAYN
260 270 280 290 300
NRAQAEIKLQ RWSSALEDCE KALELDPGNV KALLRRATTY KHQNKLQEAV
310 320 330 340 350
DDLRKVLQVE PDNDLAKKTL SEVERDLKNS EPVSELQTKG KRMVIEEVEN
360 370 380 390 400
SGDEGGKGSA DEREDGGSDE AAMGNIQKKL MVRRSEGGRR SRRGRTPGPR
410 420 430 440 450
AEQQGGLRET ATASTGDSHY PEEPRAADNP SGLKRRGNEL FRGGQFAEAA
460 470 480 490 500
AQYSVAIAQL EPTGSANADE LSILYSNRAA CYLKEGNCRD CIQDCNRALE
510 520 530 540 550
LHPFSVKPLL RRAMAYETLE QYRNAYVDYK TVLQIDCGIQ LASDSANRIA
560 570 580 590 600
RILTELDGSK WRERLPPIPA VPTSEPLRVW LPAAETPDQD PCPNNCMPSI
610 620 630 640 650
TDEKMFQALK EEGNQLVKDK NYKDAISKYN ECLKINSKAC AIYTNRALCY
660 670 680 690 700
LKLGQFEEAK LDCEQALQID GENVKASHRL ALAQKGLENC RESGVDPSQV
710 720 730 740 750
LLSPDSSEAA RHLDTKNDTA PPSKGRERRR IQVQEVDGSS DEEPERPAEA
760 770 780 790 800
SATSAPARDG VEDGGSAEPA EKLDVSKPTN AYEFGQVLST ISARKDEEAC
810 820 830 840 850
AHLLAITAPK DLPLLLSNKL EGDTFLLLIQ SLKSHLVAKD PSLVYEHLLY
860 870 880 890 900
LSKAERFKTM LTLINKGQKE QMAQLFDGLS DTQSDGLTAE DVQALRRQYE

L
Length:901
Mass (Da):100,670
Last modified:June 1, 2003 - v1
Checksum:iC8D14100AD00D69F
GO
Isoform 2 (identifier: Q80ZX8-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-372: Missing.

Show »
Length:529
Mass (Da):58,511
Checksum:i8230355A0D615C8C
GO
Isoform 3 (identifier: Q80ZX8-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-233: Missing.

Note: No experimental confirmation available.
Show »
Length:668
Mass (Da):73,972
Checksum:i0BD1BC0558F357F8
GO

Sequence cautioni

The sequence BAC27944.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti115 – 1151C → R in AAF06160 (PubMed:10527845).Curated
Sequence conflicti364 – 3641E → A in AAF06160 (PubMed:10527845).Curated
Sequence conflicti419 – 4191H → Y in AAF06160 (PubMed:10527845).Curated
Sequence conflicti419 – 4191H → Y in AAF06161 (PubMed:10527845).Curated
Sequence conflicti664 – 6641E → D in AAF06160 (PubMed:10527845).Curated
Sequence conflicti664 – 6641E → D in AAF06161 (PubMed:10527845).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 372372Missing in isoform 2. 1 PublicationVSP_012684Add
BLAST
Alternative sequencei1 – 233233Missing in isoform 3. 1 PublicationVSP_012685Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF181252 mRNA. Translation: AAF06160.1.
AF181253 mRNA. Translation: AAF06161.1.
AK032601 mRNA. Translation: BAC27944.1. Different initiation.
BC046313 mRNA. Translation: AAH46313.1.
CCDSiCCDS37061.1. [Q80ZX8-1]
PIRiJC7111.
RefSeqiNP_036161.2. NM_012031.3. [Q80ZX8-1]
XP_006520138.1. XM_006520075.2. [Q80ZX8-1]
XP_006520139.1. XM_006520076.2. [Q80ZX8-1]
XP_006520141.1. XM_006520078.2. [Q80ZX8-3]
XP_006520142.1. XM_006520079.2. [Q80ZX8-3]
XP_011243659.1. XM_011245357.1. [Q80ZX8-1]
UniGeneiMm.295539.

Genome annotation databases

EnsembliENSMUST00000047348; ENSMUSP00000047335; ENSMUSG00000037617. [Q80ZX8-1]
ENSMUST00000171205; ENSMUSP00000132233; ENSMUSG00000037617. [Q80ZX8-1]
GeneIDi26942.
KEGGimmu:26942.
UCSCiuc007vmn.1. mouse. [Q80ZX8-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF181252 mRNA. Translation: AAF06160.1.
AF181253 mRNA. Translation: AAF06161.1.
AK032601 mRNA. Translation: BAC27944.1. Different initiation.
BC046313 mRNA. Translation: AAH46313.1.
CCDSiCCDS37061.1. [Q80ZX8-1]
PIRiJC7111.
RefSeqiNP_036161.2. NM_012031.3. [Q80ZX8-1]
XP_006520138.1. XM_006520075.2. [Q80ZX8-1]
XP_006520139.1. XM_006520076.2. [Q80ZX8-1]
XP_006520141.1. XM_006520078.2. [Q80ZX8-3]
XP_006520142.1. XM_006520079.2. [Q80ZX8-3]
XP_011243659.1. XM_011245357.1. [Q80ZX8-1]
UniGeneiMm.295539.

3D structure databases

ProteinModelPortaliQ80ZX8.
SMRiQ80ZX8. Positions 143-328, 432-573, 604-737.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi205078. 1 interaction.
STRINGi10090.ENSMUSP00000047335.

PTM databases

iPTMnetiQ80ZX8.
PhosphoSiteiQ80ZX8.

Proteomic databases

EPDiQ80ZX8.
MaxQBiQ80ZX8.
PaxDbiQ80ZX8.
PRIDEiQ80ZX8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000047348; ENSMUSP00000047335; ENSMUSG00000037617. [Q80ZX8-1]
ENSMUST00000171205; ENSMUSP00000132233; ENSMUSG00000037617. [Q80ZX8-1]
GeneIDi26942.
KEGGimmu:26942.
UCSCiuc007vmn.1. mouse. [Q80ZX8-1]

Organism-specific databases

CTDi6674.
MGIiMGI:1349387. Spag1.

Phylogenomic databases

eggNOGiKOG1124. Eukaryota.
COG0457. LUCA.
GeneTreeiENSGT00840000129761.
HOGENOMiHOG000293399.
HOVERGENiHBG079204.
InParanoidiQ80ZX8.
KOiK19870.
OMAiPLQAWHP.
OrthoDBiEOG75QR4W.
PhylomeDBiQ80ZX8.
TreeFamiTF106251.

Miscellaneous databases

PROiQ80ZX8.
SOURCEiSearch...

Gene expression databases

BgeeiQ80ZX8.
GenevisibleiQ80ZX8. MM.

Family and domain databases

Gene3Di1.25.40.10. 3 hits.
InterProiIPR025986. RPAP3-like_C.
IPR013026. TPR-contain_dom.
IPR011990. TPR-like_helical_dom.
IPR001440. TPR_1.
IPR019734. TPR_repeat.
[Graphical view]
PfamiPF13877. RPAP3_C. 1 hit.
PF00515. TPR_1. 2 hits.
PF13181. TPR_8. 1 hit.
[Graphical view]
SMARTiSM00028. TPR. 8 hits.
[Graphical view]
SUPFAMiSSF48452. SSF48452. 3 hits.
PROSITEiPS50005. TPR. 6 hits.
PS50293. TPR_REGION. 4 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A tetratricopeptide repeat-containing protein gene, tpis, whose expression is induced with differentiation of spermatogenic cells."
    Takaishi M., Huh N.-H.
    Biochem. Biophys. Res. Commun. 264:81-85(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), DEVELOPMENTAL STAGE, TISSUE SPECIFICITY.
    Strain: ICR.
    Tissue: Fetal skin and Testis.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
    Strain: C57BL/6J.
    Tissue: Olfactory bulb.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Olfactory epithelium.
  4. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-351; SER-359; SER-739 AND SER-740, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Kidney, Lung and Testis.

Entry informationi

Entry nameiSPAG1_MOUSE
AccessioniPrimary (citable) accession number: Q80ZX8
Secondary accession number(s): Q8CCK7, Q9QZJ3, Q9QZJ4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 2005
Last sequence update: June 1, 2003
Last modified: June 8, 2016
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Antibodies against SPAG1 interfere with fertilization.By similarity

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.