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Q80ZI6

- LRSM1_MOUSE

UniProt

Q80ZI6 - LRSM1_MOUSE

Protein

E3 ubiquitin-protein ligase LRSAM1

Gene

Lrsam1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 102 (01 Oct 2014)
      Sequence version 1 (01 Jun 2003)
      Previous versions | rss
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    Functioni

    E3 ubiquitin-protein ligase that mediates monoubiquitination of TSG101 at multiple sites, leading to inactivate the ability of TSG101 to sort endocytic (EGF receptors) cargos.By similarity

    Pathwayi

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri679 – 71436RING-typePROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. ligase activity Source: UniProtKB-KW
    2. ubiquitin-protein transferase activity Source: UniProtKB
    3. zinc ion binding Source: InterPro

    GO - Biological processi

    1. negative regulation of endocytosis Source: UniProtKB
    2. protein autoubiquitination Source: UniProtKB
    3. protein catabolic process Source: UniProtKB
    4. protein polyubiquitination Source: UniProtKB
    5. ubiquitin-dependent endocytosis Source: UniProtKB
    6. viral budding Source: UniProtKB

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    Protein transport, Transport, Ubl conjugation pathway

    Keywords - Ligandi

    Metal-binding, Zinc

    Enzyme and pathway databases

    ReactomeiREACT_199115. Antigen processing: Ubiquitination & Proteasome degradation.
    UniPathwayiUPA00143.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    E3 ubiquitin-protein ligase LRSAM1 (EC:6.3.2.-)
    Alternative name(s):
    Leucine-rich repeat and sterile alpha motif-containing protein 1
    Tsg101-associated ligase
    Gene namesi
    Name:Lrsam1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 2

    Organism-specific databases

    MGIiMGI:2684789. Lrsam1.

    Subcellular locationi

    Cytoplasm By similarity
    Note: Displays a punctuate distribution and localizes to a submembranal ring.By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB
    2. membrane Source: UniProtKB

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 727727E3 ubiquitin-protein ligase LRSAM1PRO_0000055924Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei234 – 2341PhosphoserineBy similarity
    Modified residuei604 – 6041PhosphoserineBy similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ80ZI6.
    PaxDbiQ80ZI6.
    PRIDEiQ80ZI6.

    PTM databases

    PhosphoSiteiQ80ZI6.

    Expressioni

    Tissue specificityi

    Widely expressed.1 Publication

    Gene expression databases

    BgeeiQ80ZI6.
    GenevestigatoriQ80ZI6.

    Interactioni

    Subunit structurei

    Interacts with TSG101.By similarity

    Protein-protein interaction databases

    IntActiQ80ZI6. 1 interaction.

    Structurei

    3D structure databases

    ProteinModelPortaliQ80ZI6.
    SMRiQ80ZI6. Positions 34-216, 679-724.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati30 – 5122LRR 1Add
    BLAST
    Repeati56 – 7722LRR 2Add
    BLAST
    Repeati82 – 10322LRR 3Add
    BLAST
    Repeati105 – 12622LRR 4Add
    BLAST
    Repeati128 – 15023LRR 5Add
    BLAST
    Repeati151 – 17222LRR 6Add
    BLAST
    Domaini569 – 63264SAMPROSITE-ProRule annotationAdd
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili241 – 382142Sequence AnalysisAdd
    BLAST
    Coiled coili469 – 54779Sequence AnalysisAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi653 – 6564PTAP motif 1
    Motifi665 – 6684PTAP motif 2

    Domaini

    The coiled coil domains interact with the SB domain of TSG101.By similarity
    The PTAP motifs mediate the binding to UEV domains.By similarity

    Sequence similaritiesi

    Contains 6 LRR (leucine-rich) repeats.Curated
    Contains 1 RING-type zinc finger.PROSITE-ProRule annotation
    Contains 1 SAM (sterile alpha motif) domain.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri679 – 71436RING-typePROSITE-ProRule annotationAdd
    BLAST

    Keywords - Domaini

    Coiled coil, Leucine-rich repeat, Repeat, Zinc-finger

    Phylogenomic databases

    eggNOGiCOG4886.
    GeneTreeiENSGT00750000117546.
    HOGENOMiHOG000231972.
    HOVERGENiHBG052363.
    InParanoidiQ80ZI6.
    KOiK10641.
    OMAiIFLNCGH.
    OrthoDBiEOG77T149.
    PhylomeDBiQ80ZI6.
    TreeFamiTF329645.

    Family and domain databases

    Gene3Di1.10.150.50. 1 hit.
    3.30.40.10. 1 hit.
    InterProiIPR001611. Leu-rich_rpt.
    IPR025875. Leu-rich_rpt_4.
    IPR001660. SAM.
    IPR013761. SAM/pointed.
    IPR011510. SAM_2.
    IPR001841. Znf_RING.
    IPR013083. Znf_RING/FYVE/PHD.
    [Graphical view]
    PfamiPF12799. LRR_4. 2 hits.
    PF07647. SAM_2. 1 hit.
    [Graphical view]
    SMARTiSM00184. RING. 1 hit.
    SM00454. SAM. 1 hit.
    [Graphical view]
    SUPFAMiSSF47769. SSF47769. 1 hit.
    PROSITEiPS51450. LRR. 4 hits.
    PS50105. SAM_DOMAIN. 1 hit.
    PS50089. ZF_RING_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q80ZI6-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPLFFRKRKP SEEARKRLEY QMCLAKEAGA DDILDISKCE LSEIPFGAFA    50
    TCKVLQKKVL IVHTNHLTSL LPKSCSLLSL VTIKVLDLHE NQLTALPDDM 100
    GQLTVLQVLN VERNQLTHLP RSIGNLLQLQ TLNVKDNKLK ELPDTLGELR 150
    SLRTLDISEN EIQRLPQMLA HVRTLETLSL NALAMVYPPP EVCGAGTAAV 200
    QQFLCKESGL DYYPPSQYLL PVLEQDGAEN TQDSPDGPAS RFSREEAEWQ 250
    NRFSDYEKRK EQKMLEKLEF ERRLDLGQRE HAELLQQSHS HKDEILQTVK 300
    QEQTRLEQDL SERQRCLDAE RQQLQEQLKQ TEQSIASRIQ RLLQDNQRQK 350
    KSSEILKSLE NERIRMEQLM SITQEETENL RQREIAAAMQ QMLTESCKSR 400
    LIQMAYESQR QSLAQQACSS MAEMDKRFQQ ILSWQQMDQN KAISQILQES 450
    VMQKAAFEAL QVKKDLMHRQ IRNQIRLIET ELLQLTQLEL KRKSLDTETL 500
    QEMVSEQRWA LSNLLQQLLK EKKQREEELH GILAELEAKS ETKQENYWLI 550
    QYQRLLNQKP LSLKLQEEGM ERRLVALLVE LSAEHYLPLF AHHRISLDML 600
    SRMSPGDLAK VGVSEAGLQH EILRRAQDLL AVPRVQPELK PLENEVLGAL 650
    EPPTAPRELQ ESVRPSAPPA ELDMPTSECV VCLEREAQMV FLTCGHVCCC 700
    QQCCQPLRTC PLCRQEISQR LRIYHSS 727
    Length:727
    Mass (Da):83,976
    Last modified:June 1, 2003 - v1
    Checksum:i561B6FE6F730ADAE
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC049146 mRNA. Translation: AAH49146.1.
    CCDSiCCDS15935.1.
    RefSeqiNP_955006.1. NM_199302.2.
    XP_006498032.1. XM_006497969.1.
    XP_006498033.1. XM_006497970.1.
    XP_006498034.1. XM_006497971.1.
    XP_006498035.1. XM_006497972.1.
    UniGeneiMm.248037.

    Genome annotation databases

    EnsembliENSMUST00000028132; ENSMUSP00000028132; ENSMUSG00000026792.
    ENSMUST00000113200; ENSMUSP00000108825; ENSMUSG00000026792.
    GeneIDi227738.
    KEGGimmu:227738.
    UCSCiuc008jhd.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC049146 mRNA. Translation: AAH49146.1 .
    CCDSi CCDS15935.1.
    RefSeqi NP_955006.1. NM_199302.2.
    XP_006498032.1. XM_006497969.1.
    XP_006498033.1. XM_006497970.1.
    XP_006498034.1. XM_006497971.1.
    XP_006498035.1. XM_006497972.1.
    UniGenei Mm.248037.

    3D structure databases

    ProteinModelPortali Q80ZI6.
    SMRi Q80ZI6. Positions 34-216, 679-724.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q80ZI6. 1 interaction.

    PTM databases

    PhosphoSitei Q80ZI6.

    Proteomic databases

    MaxQBi Q80ZI6.
    PaxDbi Q80ZI6.
    PRIDEi Q80ZI6.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000028132 ; ENSMUSP00000028132 ; ENSMUSG00000026792 .
    ENSMUST00000113200 ; ENSMUSP00000108825 ; ENSMUSG00000026792 .
    GeneIDi 227738.
    KEGGi mmu:227738.
    UCSCi uc008jhd.2. mouse.

    Organism-specific databases

    CTDi 90678.
    MGIi MGI:2684789. Lrsam1.

    Phylogenomic databases

    eggNOGi COG4886.
    GeneTreei ENSGT00750000117546.
    HOGENOMi HOG000231972.
    HOVERGENi HBG052363.
    InParanoidi Q80ZI6.
    KOi K10641.
    OMAi IFLNCGH.
    OrthoDBi EOG77T149.
    PhylomeDBi Q80ZI6.
    TreeFami TF329645.

    Enzyme and pathway databases

    UniPathwayi UPA00143 .
    Reactomei REACT_199115. Antigen processing: Ubiquitination & Proteasome degradation.

    Miscellaneous databases

    NextBioi 378812.
    PROi Q80ZI6.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q80ZI6.
    Genevestigatori Q80ZI6.

    Family and domain databases

    Gene3Di 1.10.150.50. 1 hit.
    3.30.40.10. 1 hit.
    InterProi IPR001611. Leu-rich_rpt.
    IPR025875. Leu-rich_rpt_4.
    IPR001660. SAM.
    IPR013761. SAM/pointed.
    IPR011510. SAM_2.
    IPR001841. Znf_RING.
    IPR013083. Znf_RING/FYVE/PHD.
    [Graphical view ]
    Pfami PF12799. LRR_4. 2 hits.
    PF07647. SAM_2. 1 hit.
    [Graphical view ]
    SMARTi SM00184. RING. 1 hit.
    SM00454. SAM. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47769. SSF47769. 1 hit.
    PROSITEi PS51450. LRR. 4 hits.
    PS50105. SAM_DOMAIN. 1 hit.
    PS50089. ZF_RING_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Olfactory epithelium.
    2. "Tal, a Tsg101-specific E3 ubiquitin ligase, regulates receptor endocytosis and retrovirus budding."
      Amit I., Yakir L., Katz M., Zwang Y., Marmor M.D., Citri A., Shtiegman K., Alroy I., Tuvia S., Reiss Y., Roubini E., Cohen M., Wides R., Bacharach E., Schubert U., Yarden Y.
      Genes Dev. 18:1737-1752(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY.

    Entry informationi

    Entry nameiLRSM1_MOUSE
    AccessioniPrimary (citable) accession number: Q80ZI6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 2005
    Last sequence update: June 1, 2003
    Last modified: October 1, 2014
    This is version 102 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3