Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

E3 ubiquitin-protein ligase LRSAM1

Gene

Lrsam1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

E3 ubiquitin-protein ligase that mediates monoubiquitination of TSG101 at multiple sites, leading to inactivate the ability of TSG101 to sort endocytic (EGF receptors) cargos.By similarity

Pathway:iprotein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri679 – 71436RING-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Protein transport, Transport, Ubl conjugation pathway

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiREACT_343568. Antigen processing: Ubiquitination & Proteasome degradation.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase LRSAM1 (EC:6.3.2.-)
Alternative name(s):
Leucine-rich repeat and sterile alpha motif-containing protein 1
Tsg101-associated ligase
Gene namesi
Name:Lrsam1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:2684789. Lrsam1.

Subcellular locationi

  • Cytoplasm By similarity

  • Note: Displays a punctuate distribution and localizes to a submembranal ring.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 727727E3 ubiquitin-protein ligase LRSAM1PRO_0000055924Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei234 – 2341PhosphoserineBy similarity
Modified residuei604 – 6041PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ80ZI6.
PaxDbiQ80ZI6.
PRIDEiQ80ZI6.

PTM databases

PhosphoSiteiQ80ZI6.

Expressioni

Tissue specificityi

Widely expressed.1 Publication

Gene expression databases

BgeeiQ80ZI6.
ExpressionAtlasiQ80ZI6. baseline and differential.
GenevisibleiQ80ZI6. MM.

Interactioni

Subunit structurei

Interacts with TSG101.By similarity

Protein-protein interaction databases

IntActiQ80ZI6. 1 interaction.
STRINGi10090.ENSMUSP00000028132.

Structurei

3D structure databases

ProteinModelPortaliQ80ZI6.
SMRiQ80ZI6. Positions 34-216, 679-724.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati30 – 5122LRR 1Add
BLAST
Repeati56 – 7722LRR 2Add
BLAST
Repeati82 – 10322LRR 3Add
BLAST
Repeati105 – 12622LRR 4Add
BLAST
Repeati128 – 15023LRR 5Add
BLAST
Repeati151 – 17222LRR 6Add
BLAST
Domaini569 – 63264SAMPROSITE-ProRule annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili241 – 382142Sequence AnalysisAdd
BLAST
Coiled coili469 – 54779Sequence AnalysisAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi653 – 6564PTAP motif 1
Motifi665 – 6684PTAP motif 2

Domaini

The coiled coil domains interact with the SB domain of TSG101.By similarity
The PTAP motifs mediate the binding to UEV domains.By similarity

Sequence similaritiesi

Contains 6 LRR (leucine-rich) repeats.Curated
Contains 1 RING-type zinc finger.PROSITE-ProRule annotation
Contains 1 SAM (sterile alpha motif) domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri679 – 71436RING-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Coiled coil, Leucine-rich repeat, Repeat, Zinc-finger

Phylogenomic databases

eggNOGiCOG4886.
GeneTreeiENSGT00800000124027.
HOGENOMiHOG000231972.
HOVERGENiHBG052363.
InParanoidiQ80ZI6.
KOiK10641.
OMAiFGAFATC.
OrthoDBiEOG77T149.
PhylomeDBiQ80ZI6.
TreeFamiTF329645.

Family and domain databases

Gene3Di1.10.150.50. 1 hit.
3.30.40.10. 1 hit.
InterProiIPR001611. Leu-rich_rpt.
IPR025875. Leu-rich_rpt_4.
IPR001660. SAM.
IPR013761. SAM/pointed.
IPR011510. SAM_2.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PfamiPF12799. LRR_4. 2 hits.
PF07647. SAM_2. 1 hit.
[Graphical view]
SMARTiSM00184. RING. 1 hit.
SM00454. SAM. 1 hit.
[Graphical view]
SUPFAMiSSF47769. SSF47769. 1 hit.
PROSITEiPS51450. LRR. 4 hits.
PS50105. SAM_DOMAIN. 1 hit.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q80ZI6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPLFFRKRKP SEEARKRLEY QMCLAKEAGA DDILDISKCE LSEIPFGAFA
60 70 80 90 100
TCKVLQKKVL IVHTNHLTSL LPKSCSLLSL VTIKVLDLHE NQLTALPDDM
110 120 130 140 150
GQLTVLQVLN VERNQLTHLP RSIGNLLQLQ TLNVKDNKLK ELPDTLGELR
160 170 180 190 200
SLRTLDISEN EIQRLPQMLA HVRTLETLSL NALAMVYPPP EVCGAGTAAV
210 220 230 240 250
QQFLCKESGL DYYPPSQYLL PVLEQDGAEN TQDSPDGPAS RFSREEAEWQ
260 270 280 290 300
NRFSDYEKRK EQKMLEKLEF ERRLDLGQRE HAELLQQSHS HKDEILQTVK
310 320 330 340 350
QEQTRLEQDL SERQRCLDAE RQQLQEQLKQ TEQSIASRIQ RLLQDNQRQK
360 370 380 390 400
KSSEILKSLE NERIRMEQLM SITQEETENL RQREIAAAMQ QMLTESCKSR
410 420 430 440 450
LIQMAYESQR QSLAQQACSS MAEMDKRFQQ ILSWQQMDQN KAISQILQES
460 470 480 490 500
VMQKAAFEAL QVKKDLMHRQ IRNQIRLIET ELLQLTQLEL KRKSLDTETL
510 520 530 540 550
QEMVSEQRWA LSNLLQQLLK EKKQREEELH GILAELEAKS ETKQENYWLI
560 570 580 590 600
QYQRLLNQKP LSLKLQEEGM ERRLVALLVE LSAEHYLPLF AHHRISLDML
610 620 630 640 650
SRMSPGDLAK VGVSEAGLQH EILRRAQDLL AVPRVQPELK PLENEVLGAL
660 670 680 690 700
EPPTAPRELQ ESVRPSAPPA ELDMPTSECV VCLEREAQMV FLTCGHVCCC
710 720
QQCCQPLRTC PLCRQEISQR LRIYHSS
Length:727
Mass (Da):83,976
Last modified:June 1, 2003 - v1
Checksum:i561B6FE6F730ADAE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC049146 mRNA. Translation: AAH49146.1.
CCDSiCCDS15935.1.
RefSeqiNP_955006.1. NM_199302.2.
XP_006498032.1. XM_006497969.2.
XP_006498033.1. XM_006497970.2.
XP_006498034.1. XM_006497971.2.
XP_006498035.1. XM_006497972.2.
UniGeneiMm.248037.

Genome annotation databases

EnsembliENSMUST00000028132; ENSMUSP00000028132; ENSMUSG00000026792.
ENSMUST00000113200; ENSMUSP00000108825; ENSMUSG00000026792.
GeneIDi227738.
KEGGimmu:227738.
UCSCiuc008jhd.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC049146 mRNA. Translation: AAH49146.1.
CCDSiCCDS15935.1.
RefSeqiNP_955006.1. NM_199302.2.
XP_006498032.1. XM_006497969.2.
XP_006498033.1. XM_006497970.2.
XP_006498034.1. XM_006497971.2.
XP_006498035.1. XM_006497972.2.
UniGeneiMm.248037.

3D structure databases

ProteinModelPortaliQ80ZI6.
SMRiQ80ZI6. Positions 34-216, 679-724.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ80ZI6. 1 interaction.
STRINGi10090.ENSMUSP00000028132.

PTM databases

PhosphoSiteiQ80ZI6.

Proteomic databases

MaxQBiQ80ZI6.
PaxDbiQ80ZI6.
PRIDEiQ80ZI6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000028132; ENSMUSP00000028132; ENSMUSG00000026792.
ENSMUST00000113200; ENSMUSP00000108825; ENSMUSG00000026792.
GeneIDi227738.
KEGGimmu:227738.
UCSCiuc008jhd.2. mouse.

Organism-specific databases

CTDi90678.
MGIiMGI:2684789. Lrsam1.

Phylogenomic databases

eggNOGiCOG4886.
GeneTreeiENSGT00800000124027.
HOGENOMiHOG000231972.
HOVERGENiHBG052363.
InParanoidiQ80ZI6.
KOiK10641.
OMAiFGAFATC.
OrthoDBiEOG77T149.
PhylomeDBiQ80ZI6.
TreeFamiTF329645.

Enzyme and pathway databases

UniPathwayiUPA00143.
ReactomeiREACT_343568. Antigen processing: Ubiquitination & Proteasome degradation.

Miscellaneous databases

NextBioi378812.
PROiQ80ZI6.
SOURCEiSearch...

Gene expression databases

BgeeiQ80ZI6.
ExpressionAtlasiQ80ZI6. baseline and differential.
GenevisibleiQ80ZI6. MM.

Family and domain databases

Gene3Di1.10.150.50. 1 hit.
3.30.40.10. 1 hit.
InterProiIPR001611. Leu-rich_rpt.
IPR025875. Leu-rich_rpt_4.
IPR001660. SAM.
IPR013761. SAM/pointed.
IPR011510. SAM_2.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PfamiPF12799. LRR_4. 2 hits.
PF07647. SAM_2. 1 hit.
[Graphical view]
SMARTiSM00184. RING. 1 hit.
SM00454. SAM. 1 hit.
[Graphical view]
SUPFAMiSSF47769. SSF47769. 1 hit.
PROSITEiPS51450. LRR. 4 hits.
PS50105. SAM_DOMAIN. 1 hit.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Olfactory epithelium.
  2. "Tal, a Tsg101-specific E3 ubiquitin ligase, regulates receptor endocytosis and retrovirus budding."
    Amit I., Yakir L., Katz M., Zwang Y., Marmor M.D., Citri A., Shtiegman K., Alroy I., Tuvia S., Reiss Y., Roubini E., Cohen M., Wides R., Bacharach E., Schubert U., Yarden Y.
    Genes Dev. 18:1737-1752(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.

Entry informationi

Entry nameiLRSM1_MOUSE
AccessioniPrimary (citable) accession number: Q80ZI6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 2005
Last sequence update: June 1, 2003
Last modified: July 22, 2015
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.