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Q80ZG2 (IP3KC_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified September 21, 2011. Version 52. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Inositol-trisphosphate 3-kinase C
EC=2.7.1.127
Alternative name(s):
Inositol 1,4,5-trisphosphate 3-kinase C
Short name=IP3 3-kinase C
Short name=IP3K C
Short name=InsP 3-kinase C
Gene names
Name:Itpkc
Synonyms:Ip3kc
OrganismRattus norvegicus (Rat)
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length678 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Can phosphorylate inositol 2,4,5-triphosphate to inositol 2,4,5,6-tetraphosphate. Ref.1

Catalytic activity

ATP + 1D-myo-inositol 1,4,5-trisphosphate = ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate.

Enzyme regulation

Activated by Ca2+/calmodulin. Inhibited by high concentrations of the substrate Ins(1,2,4)P3, and allosterically activated by the product Ins(1,3,4,5)P4.

Subcellular location

Nucleus. Cytoplasm. Note: Shuttles actively between nucleus and cytoplasm with both nuclear import and nuclear export activity. Ref.1

Tissue specificity

Highly expressed in tongue epithelium, taste papilla, heart, brain and testis. Weakly expressed in lung, liver and kidney. Ref.1

Sequence similarities

Belongs to the inositol phosphokinase (IPK) family.

Biophysicochemical properties

Kinetic parameters:

KM=0.2 µM for Ins(1,4,5)P3 Ref.1

KM=1.5 µM for Ins(2,4,5)P3

KM=33 µM for ATP

Ontologies

Keywords
   Cellular componentCytoplasm
Nucleus
   LigandATP-binding
Calmodulin-binding
Nucleotide-binding
   Molecular functionKinase
Transferase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

nucleus

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

calmodulin binding

Inferred from electronic annotation. Source: UniProtKB-KW

inositol-1,4,5-trisphosphate 3-kinase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 678678Inositol-trisphosphate 3-kinase C
PRO_0000234072

Regions

Nucleotide binding466 – 4683ATP By similarity
Region318 – 3269Nuclear export signal
Region502 – 5087Substrate binding By similarity
Region504 – 5129Calmodulin-binding By similarity
Region529 – 5368Substrate binding By similarity

Sites

Binding site4261ATP By similarity
Binding site4791ATP By similarity
Binding site4811Substrate By similarity
Binding site5531ATP By similarity
Binding site6331ATP By similarity
Binding site6361Substrate By similarity

Experimental info

Mutagenesis318 – 3269Missing: No export in the cytoplasm. Ref.1
Mutagenesis318 – 3214LCPV → ACPA: No export in the cytoplasm. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q80ZG2 [UniParc].

Last modified June 1, 2003. Version 1.
Checksum: 5EDEA14255DACD2B

FASTA67874,463
        10         20         30         40         50         60 
MRRCPCRGSL SEAEAGALPA EARMGLEALR GGRRRQPGLQ RPGPGAGGPT GRPEGGGPRA 

        70         80         90        100        110        120 
WIEGFSLHSE AERTDFGPAP CPDGPQAEPC GDEHEECEAA GLGVASEKPS QNKELDGSNL 

       130        140        150        160        170        180 
QTHPKLSSPL AEMEMAGSWT DGFRTDLHRP DLQARPKRAS LCTQPGFDES WTELDRSELW 

       190        200        210        220        230        240 
QTLPERDKPW VDHLRTHQDM SRLQNHPACP SPEPSAGTSC KELSADGSRT PHDTDGFWIE 

       250        260        270        280        290        300 
SQTDGSLIGP STQTACRQPA NDGFSAQDTD GTLIQPGTDD GPWVDSVLEK SNGDDPLMEP 

       310        320        330        340        350        360 
EPRDLVTNLC SHLECSSLCP VPRLIITSES PEPGAQPLGP QARIEGGTGG FSSASSFDES 

       370        380        390        400        410        420 
EDDLVAGGGG TSDPEDRSGS KPWKKLKTVL KYSPFVVSFH KHYYPWVQLS GHAGNFQAGE 

       430        440        450        460        470        480 
DGRILKRFCQ CEQRSLELLM GDPLRPFVPT YYGMVQRDGQ AFNQMEDLLA DFEGPSIMDC 

       490        500        510        520        530        540 
KMGSRTYLEE ELVKARERPK PRKDMYEKMV AVDPGAPTPE EHAQGAVTKP RYMQWRETLS 

       550        560        570        580        590        600 
STSTLGFRIE GIKKADGTCN TNFKKTQALE QVTKVLEDFV NGDVGILRKY VARLEDLRDT 

       610        620        630        640        650        660 
LENSPFFKTH EVVGSSLLFV HDHTGLAKVW MIDFGKTVAL PDHQMLSHRL PWAEGNREDG 

       670 
YLWGLDNLIC LLQGLAQS

« Hide

References

[1]"Rat inositol 1,4,5-trisphosphate 3-kinase C is enzymatically specialized for basal cellular inositol trisphosphate phosphorylation and shuttles actively between nucleus and cytoplasm."
Nalaskowski M.M., Bertsch U., Fanick W., Stockebrand M.C., Schmale H., Mayr G.W.
J. Biol. Chem. 278:19765-19776(2003) [PubMed: 12649294] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, FUNCTION, MUTAGENESIS OF 318-LEU--VAL-321 AND 318-LEU--ILE-326.
Strain: Wistar.
Tissue: Circumvallate papilla.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AY160770 mRNA. Translation: AAO20335.1.
AJ440782 mRNA. Translation: CAD29464.1.
IPIIPI00331949.
RefSeqNP_835195.1. NM_178094.3.
UniGeneRn.85907.

3D structure databases

ProteinModelPortalQ80ZG2.
SMRQ80ZG2. Positions 389-678.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000018696; ENSRNOP00000018696; ENSRNOG00000013945.
GeneID308451.
KEGGrno:308451.
UCSCNM_178094. rat.

Organism-specific databases

CTD80271.
RGD631336. Itpkc.

Phylogenomic databases

eggNOGroNOG12623.
GeneTreeENSGT00390000017438.
HOVERGENHBG081804.
InParanoidQ80ZG2.
OMADNLWTHQ.
OrthoDBEOG476JZS.
PhylomeDBQ80ZG2.

Gene expression databases

ArrayExpressQ80ZG2.
GenevestigatorQ80ZG2.
GermOnlineENSRNOG00000013945. Rattus norvegicus.

Family and domain databases

InterProIPR005522. IPK.
[Graphical view]
PANTHERPTHR12400. IPK. 1 hit.
PfamPF03770. IPK. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio658916.

Entry information

Entry nameIP3KC_RAT
AccessionPrimary (citable) accession number: Q80ZG2
Entry history
Integrated into UniProtKB/Swiss-Prot: May 2, 2006
Last sequence update: June 1, 2003
Last modified: September 21, 2011
This is version 52 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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