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Protein

Inositol-trisphosphate 3-kinase C

Gene

Itpkc

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Can phosphorylate inositol 2,4,5-triphosphate to inositol 2,4,5,6-tetraphosphate.1 Publication

Catalytic activityi

ATP + 1D-myo-inositol 1,4,5-trisphosphate = ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate.

Enzyme regulationi

Activated by Ca2+/calmodulin. Inhibited by high concentrations of the substrate Ins(1,2,4)P3, and allosterically activated by the product Ins(1,3,4,5)P4.

Kineticsi

  1. KM=0.2 µM for Ins(1,4,5)P31 Publication
  2. KM=1.5 µM for Ins(2,4,5)P31 Publication
  3. KM=33 µM for ATP1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei426 – 4261ATPBy similarity
    Binding sitei479 – 4791ATPBy similarity
    Binding sitei481 – 4811SubstrateBy similarity
    Binding sitei553 – 5531ATPBy similarity
    Binding sitei633 – 6331ATPBy similarity
    Binding sitei636 – 6361SubstrateBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi466 – 4683ATPBy similarity

    GO - Molecular functioni

    • ATP binding Source: UniProtKB-KW
    • inositol-1,4,5-trisphosphate 3-kinase activity Source: UniProtKB-EC
    • kinase activity Source: RGD

    GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Ligandi

    ATP-binding, Calmodulin-binding, Nucleotide-binding

    Enzyme and pathway databases

    BRENDAi2.7.1.127. 5301.
    ReactomeiR-RNO-1855204. Synthesis of IP3 and IP4 in the cytosol.
    SABIO-RKQ80ZG2.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Inositol-trisphosphate 3-kinase C (EC:2.7.1.127)
    Alternative name(s):
    Inositol 1,4,5-trisphosphate 3-kinase C
    Short name:
    IP3 3-kinase C
    Short name:
    IP3K C
    Short name:
    InsP 3-kinase C
    Gene namesi
    Name:Itpkc
    Synonyms:Ip3kc
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    Proteomesi
    • UP000002494 Componenti: Chromosome 1

    Organism-specific databases

    RGDi631336. Itpkc.

    Subcellular locationi

    • Nucleus 1 Publication
    • Cytoplasm 1 Publication

    • Note: Shuttles actively between nucleus and cytoplasm with both nuclear import and nuclear export activity.

    GO - Cellular componenti

    Complete GO annotation...

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi318 – 3269Missing : No export in the cytoplasm. 1 Publication
    Mutagenesisi318 – 3214LCPV → ACPA: No export in the cytoplasm. 1 Publication

    Chemistry

    ChEMBLiCHEMBL2667.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 678678Inositol-trisphosphate 3-kinase CPRO_0000234072Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei160 – 1601PhosphoserineBy similarity
    Modified residuei398 – 3981PhosphoserineBy similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiQ80ZG2.

    PTM databases

    iPTMnetiQ80ZG2.
    PhosphoSiteiQ80ZG2.

    Expressioni

    Tissue specificityi

    Highly expressed in tongue epithelium, taste papilla, heart, brain and testis. Weakly expressed in lung, liver and kidney.1 Publication

    Gene expression databases

    BgeeiENSRNOG00000013945.
    GenevisibleiQ80ZG2. RN.

    Interactioni

    Protein-protein interaction databases

    STRINGi10116.ENSRNOP00000018696.

    Chemistry

    BindingDBiQ80ZG2.

    Structurei

    3D structure databases

    ProteinModelPortaliQ80ZG2.
    SMRiQ80ZG2. Positions 389-678.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni502 – 5087Substrate bindingBy similarity
    Regioni504 – 5129Calmodulin-bindingBy similarity
    Regioni529 – 5368Substrate bindingBy similarity

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi318 – 3269Nuclear export signal

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiKOG1621. Eukaryota.
    ENOG410Y8AC. LUCA.
    GeneTreeiENSGT00390000017438.
    HOGENOMiHOG000113089.
    HOVERGENiHBG081804.
    InParanoidiQ80ZG2.
    KOiK00911.
    OMAiSWKELYT.
    OrthoDBiEOG091G03J3.
    PhylomeDBiQ80ZG2.
    TreeFamiTF318394.

    Family and domain databases

    InterProiIPR005522. IPK.
    [Graphical view]
    PANTHERiPTHR12400. PTHR12400. 2 hits.
    PfamiPF03770. IPK. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q80ZG2-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MRRCPCRGSL SEAEAGALPA EARMGLEALR GGRRRQPGLQ RPGPGAGGPT
    60 70 80 90 100
    GRPEGGGPRA WIEGFSLHSE AERTDFGPAP CPDGPQAEPC GDEHEECEAA
    110 120 130 140 150
    GLGVASEKPS QNKELDGSNL QTHPKLSSPL AEMEMAGSWT DGFRTDLHRP
    160 170 180 190 200
    DLQARPKRAS LCTQPGFDES WTELDRSELW QTLPERDKPW VDHLRTHQDM
    210 220 230 240 250
    SRLQNHPACP SPEPSAGTSC KELSADGSRT PHDTDGFWIE SQTDGSLIGP
    260 270 280 290 300
    STQTACRQPA NDGFSAQDTD GTLIQPGTDD GPWVDSVLEK SNGDDPLMEP
    310 320 330 340 350
    EPRDLVTNLC SHLECSSLCP VPRLIITSES PEPGAQPLGP QARIEGGTGG
    360 370 380 390 400
    FSSASSFDES EDDLVAGGGG TSDPEDRSGS KPWKKLKTVL KYSPFVVSFH
    410 420 430 440 450
    KHYYPWVQLS GHAGNFQAGE DGRILKRFCQ CEQRSLELLM GDPLRPFVPT
    460 470 480 490 500
    YYGMVQRDGQ AFNQMEDLLA DFEGPSIMDC KMGSRTYLEE ELVKARERPK
    510 520 530 540 550
    PRKDMYEKMV AVDPGAPTPE EHAQGAVTKP RYMQWRETLS STSTLGFRIE
    560 570 580 590 600
    GIKKADGTCN TNFKKTQALE QVTKVLEDFV NGDVGILRKY VARLEDLRDT
    610 620 630 640 650
    LENSPFFKTH EVVGSSLLFV HDHTGLAKVW MIDFGKTVAL PDHQMLSHRL
    660 670
    PWAEGNREDG YLWGLDNLIC LLQGLAQS
    Length:678
    Mass (Da):74,463
    Last modified:June 1, 2003 - v1
    Checksum:i5EDEA14255DACD2B
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AY160770 mRNA. Translation: AAO20335.1.
    AJ440782 mRNA. Translation: CAD29464.1.
    RefSeqiNP_835195.1. NM_178094.3.
    UniGeneiRn.85907.

    Genome annotation databases

    EnsembliENSRNOT00000018696; ENSRNOP00000018696; ENSRNOG00000013945.
    GeneIDi308451.
    KEGGirno:308451.
    UCSCiRGD:631336. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AY160770 mRNA. Translation: AAO20335.1.
    AJ440782 mRNA. Translation: CAD29464.1.
    RefSeqiNP_835195.1. NM_178094.3.
    UniGeneiRn.85907.

    3D structure databases

    ProteinModelPortaliQ80ZG2.
    SMRiQ80ZG2. Positions 389-678.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi10116.ENSRNOP00000018696.

    Chemistry

    BindingDBiQ80ZG2.
    ChEMBLiCHEMBL2667.

    PTM databases

    iPTMnetiQ80ZG2.
    PhosphoSiteiQ80ZG2.

    Proteomic databases

    PaxDbiQ80ZG2.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsembliENSRNOT00000018696; ENSRNOP00000018696; ENSRNOG00000013945.
    GeneIDi308451.
    KEGGirno:308451.
    UCSCiRGD:631336. rat.

    Organism-specific databases

    CTDi80271.
    RGDi631336. Itpkc.

    Phylogenomic databases

    eggNOGiKOG1621. Eukaryota.
    ENOG410Y8AC. LUCA.
    GeneTreeiENSGT00390000017438.
    HOGENOMiHOG000113089.
    HOVERGENiHBG081804.
    InParanoidiQ80ZG2.
    KOiK00911.
    OMAiSWKELYT.
    OrthoDBiEOG091G03J3.
    PhylomeDBiQ80ZG2.
    TreeFamiTF318394.

    Enzyme and pathway databases

    BRENDAi2.7.1.127. 5301.
    ReactomeiR-RNO-1855204. Synthesis of IP3 and IP4 in the cytosol.
    SABIO-RKQ80ZG2.

    Miscellaneous databases

    PROiQ80ZG2.

    Gene expression databases

    BgeeiENSRNOG00000013945.
    GenevisibleiQ80ZG2. RN.

    Family and domain databases

    InterProiIPR005522. IPK.
    [Graphical view]
    PANTHERiPTHR12400. PTHR12400. 2 hits.
    PfamiPF03770. IPK. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Entry informationi

    Entry nameiIP3KC_RAT
    AccessioniPrimary (citable) accession number: Q80ZG2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 2, 2006
    Last sequence update: June 1, 2003
    Last modified: September 7, 2016
    This is version 83 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.