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Protein

Amphoterin-induced protein 1

Gene

Amigo1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Promotes growth and fasciculation of neurites from cultured hippocampal neurons. May be involved in fasciculation as well as myelination of developing neural axons. May have a role in regeneration as well as neural plasticity in the adult nervous system. May mediate homophilic as well as heterophilic cell-cell interaction and contribute to signal transduction through its intracellular domain (By similarity). Assembled with KCNB1 modulates the gating characteristics of the delayed rectifier voltage-dependent potassium channel KCNB1 (PubMed:22056818).By similarity1 Publication

GO - Molecular functioni

  • potassium channel regulator activity Source: UniProtKB

GO - Biological processi

  • axonal fasciculation Source: UniProtKB
  • axonogenesis Source: MGI
  • cell adhesion Source: MGI
  • heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules Source: UniProtKB
  • homophilic cell adhesion via plasma membrane adhesion molecules Source: UniProtKB
  • myelination Source: UniProtKB
  • positive regulation of axonogenesis Source: UniProtKB
  • positive regulation of potassium ion transmembrane transport Source: UniProtKB
  • positive regulation of synapse assembly Source: MGI

Keywordsi

Molecular functionDevelopmental protein
Biological processCell adhesion, Differentiation, Neurogenesis

Names & Taxonomyi

Protein namesi
Recommended name:
Amphoterin-induced protein 1
Alternative name(s):
AMIGO-1
Alivin-2
Gene namesi
Name:Amigo1Imported
Synonyms:Ali2Imported, Amigo1 Publication, Kiaa1163Imported
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:2653612 Amigo1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini28 – 371ExtracellularSequence analysisAdd BLAST344
Transmembranei372 – 392HelicalSequence analysisAdd BLAST21
Topological domaini393 – 492CytoplasmicSequence analysisAdd BLAST100

Keywords - Cellular componenti

Cell membrane, Cell projection, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi348N → A: Prevents from leaving the ER. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 27Sequence analysisAdd BLAST27
ChainiPRO_000001450728 – 492Amphoterin-induced protein 1Sequence analysisAdd BLAST465

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi34 ↔ 40PROSITE-ProRule annotation1 Publication
Disulfide bondi38 ↔ 47PROSITE-ProRule annotation1 Publication
Glycosylationi72N-linked (GlcNAc...) asparagine1 Publication1
Disulfide bondi225 ↔ 253PROSITE-ProRule annotation1 Publication
Disulfide bondi227 ↔ 270PROSITE-ProRule annotation1 Publication
Glycosylationi269N-linked (GlcNAc...) asparagine1 Publication1
Disulfide bondi290 ↔ 340PROSITE-ProRule annotation1 Publication
Glycosylationi315N-linked (GlcNAc...) asparagine1 Publication1
Glycosylationi348N-linked (GlcNAc...) asparagine1 Publication1
Glycosylationi359N-linked (GlcNAc...) asparagine1 Publication1
Modified residuei476PhosphoserineCombined sources1
Modified residuei480PhosphoserineCombined sources1

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ80ZD8
PeptideAtlasiQ80ZD8
PRIDEiQ80ZD8

PTM databases

iPTMnetiQ80ZD8
PhosphoSitePlusiQ80ZD8

Expressioni

Tissue specificityi

Expressed in hippocampal and cortical neurons (at protein level) (PubMed:22056818). High levels in cerebellum, cerebrum, and retina. Low levels in liver, kidney, small intestine, spleen, lung and heart.2 Publications

Gene expression databases

BgeeiENSMUSG00000050947
CleanExiMM_AMIGO1
ExpressionAtlasiQ80ZD8 baseline and differential
GenevisibleiQ80ZD8 MM

Interactioni

Subunit structurei

Homodimer, and heterodimer with AMIGO2 and AMIGO3 (PubMed:21983541). Interacts with KCNB1 (PubMed:22056818).2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Kcnb1Q037174EBI-7511393,EBI-7511364

Protein-protein interaction databases

BioGridi230893, 1 interactor
IntActiQ80ZD8, 1 interactor
MINTiQ80ZD8
STRINGi10090.ENSMUSP00000061244

Structurei

Secondary structure

1492
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi39 – 41Combined sources3
Beta strandi44 – 46Combined sources3
Beta strandi64 – 67Combined sources4
Beta strandi80 – 83Combined sources4
Beta strandi90 – 92Combined sources3
Turni103 – 108Combined sources6
Beta strandi114 – 116Combined sources3
Turni127 – 132Combined sources6
Beta strandi138 – 140Combined sources3
Turni151 – 156Combined sources6
Beta strandi162 – 164Combined sources3
Helixi175 – 177Combined sources3
Beta strandi189 – 191Combined sources3
Helixi202 – 207Combined sources6
Helixi210 – 213Combined sources4
Helixi227 – 238Combined sources12
Helixi242 – 245Combined sources4
Helixi248 – 250Combined sources3
Beta strandi252 – 257Combined sources6
Helixi262 – 264Combined sources3
Beta strandi279 – 281Combined sources3
Beta strandi286 – 288Combined sources3
Beta strandi298 – 302Combined sources5
Beta strandi314 – 321Combined sources8
Beta strandi324 – 329Combined sources6
Beta strandi336 – 343Combined sources8
Beta strandi348 – 360Combined sources13

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2XOTX-ray2.00A/B28-370[»]
ProteinModelPortaliQ80ZD8
SMRiQ80ZD8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini28 – 61LRRNTAdd BLAST34
Repeati62 – 83LRR 11 PublicationAdd BLAST22
Repeati87 – 108LRR 21 PublicationAdd BLAST22
Repeati111 – 132LRR 31 PublicationAdd BLAST22
Repeati135 – 156LRR 41 PublicationAdd BLAST22
Repeati159 – 180LRR 51 PublicationAdd BLAST22
Repeati186 – 206LRR 61 PublicationAdd BLAST21
Domaini208 – 272LRRCTAdd BLAST65
Domaini269 – 352Ig-like C2-typeSequence analysisAdd BLAST84

Domaini

The LRR repeat region mediates homodimerization.

Sequence similaritiesi

Keywords - Domaini

Immunoglobulin domain, Leucine-rich repeat, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IGN4 Eukaryota
ENOG410ZZH4 LUCA
GeneTreeiENSGT00530000063545
HOGENOMiHOG000231327
HOVERGENiHBG080231
InParanoidiQ80ZD8
KOiK22529
OMAiCSEYKER
OrthoDBiEOG091G06PG
TreeFamiTF326838

Family and domain databases

Gene3Di2.60.40.10, 1 hit
3.80.10.10, 1 hit
InterProiView protein in InterPro
IPR031283 AMIGO
IPR031284 AMIGO1
IPR000483 Cys-rich_flank_reg_C
IPR007110 Ig-like_dom
IPR036179 Ig-like_dom_sf
IPR013783 Ig-like_fold
IPR013098 Ig_I-set
IPR003599 Ig_sub
IPR003598 Ig_sub2
IPR001611 Leu-rich_rpt
IPR003591 Leu-rich_rpt_typical-subtyp
IPR032675 LRR_dom_sf
PANTHERiPTHR24368 PTHR24368, 1 hit
PTHR24368:SF1 PTHR24368:SF1, 1 hit
PfamiView protein in Pfam
PF07679 I-set, 1 hit
PF13855 LRR_8, 1 hit
SMARTiView protein in SMART
SM00409 IG, 1 hit
SM00408 IGc2, 1 hit
SM00369 LRR_TYP, 5 hits
SM00082 LRRCT, 1 hit
SUPFAMiSSF48726 SSF48726, 1 hit
PROSITEiView protein in PROSITE
PS50835 IG_LIKE, 1 hit
PS51450 LRR, 6 hits

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 11 Publication (identifier: Q80ZD8-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MQPQRDLRGL WLLLLSVFLL LFEVARAGRS VVSCPANCLC ASNILSCSKQ
60 70 80 90 100
QLPNVPQSLP SYTALLDLSH NNLSRLRAEW TPTRLTNLHS LLLSHNHLNF
110 120 130 140 150
ISSEAFVPVP NLRYLDLSSN HLHTLDEFLF SDLQALEVLL LYNNHIVVVD
160 170 180 190 200
RNAFEDMAQL QKLYLSQNQI SRFPVELIKD GNKLPKLMLL DLSSNKLKKL
210 220 230 240 250
PLTDLQKLPA WVKNGLYLHN NPLECDCKLY QLFSHWQYRQ LSSVMDFQED
260 270 280 290 300
LYCMHSKKLH NIFSLDFFNC SEYKESAWEA HLGDTLTIRC DTKQQGMTKV
310 320 330 340 350
WVSPSNEQVL SQGSNGSVSV RNGDLFFKKV QVEDGGVYTC YAMGETFNET
360 370 380 390 400
LSVELKVYNF TLHGHHDTLN TAYTTLVGCI LSVVLVLIYL YLTPCRCWCR
410 420 430 440 450
GVEKPSSHQG DSLSSSMLST TPNHDPMAGG DKDDGFDRRV AFLEPAGPGQ
460 470 480 490
GQNGKLKPGN TLPVPEATGK GQRRMSDPES VSSVFSDTPI VV
Length:492
Mass (Da):55,343
Last modified:June 1, 2003 - v1
Checksum:iEF1CCD6BA61FE222
GO
Isoform 2Curated (identifier: Q80ZD8-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     290-309: CDTKQQGMTKVWVSPSNEQV → TLPPTVYTRLANLRAHGLYN
     310-492: Missing.

Note: No experimental confirmation available.Curated
Show »
Length:309
Mass (Da):35,661
Checksum:i26C3E666ECEBDA8E
GO

Sequence cautioni

The sequence AAH22907 differs from that shown. Reason: Frameshift at position 113.Curated
The sequence BAD32396 differs from that shown. Reason: Erroneous initiation.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_014166290 – 309CDTKQ…SNEQV → TLPPTVYTRLANLRAHGLYN in isoform 2. 1 PublicationAdd BLAST20
Alternative sequenceiVSP_014167310 – 492Missing in isoform 2. 1 PublicationAdd BLAST183

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY237008 mRNA Translation: AAO48949.1
AB167509 mRNA Translation: BAD12541.1
AK035960 mRNA Translation: BAC29259.1
AK173118 mRNA Translation: BAD32396.1 Different initiation.
AL671854 Genomic DNA No translation available.
BC010598 mRNA Translation: AAH10598.2
BC022907 mRNA Translation: AAH22907.1 Frameshift.
CCDSiCCDS17753.1 [Q80ZD8-1]
CCDS71305.1 [Q80ZD8-2]
RefSeqiNP_001004293.1, NM_001004293.2 [Q80ZD8-1]
NP_001274022.1, NM_001287093.1 [Q80ZD8-2]
NP_666249.2, NM_146137.3 [Q80ZD8-1]
UniGeneiMm.275752

Genome annotation databases

EnsembliENSMUST00000050909; ENSMUSP00000061244; ENSMUSG00000050947 [Q80ZD8-1]
ENSMUST00000106656; ENSMUSP00000102267; ENSMUSG00000050947 [Q80ZD8-1]
ENSMUST00000106659; ENSMUSP00000102270; ENSMUSG00000050947 [Q80ZD8-2]
GeneIDi229715
KEGGimmu:229715
UCSCiuc008qyg.2 mouse [Q80ZD8-1]
uc033hze.1 mouse [Q80ZD8-2]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiAMGO1_MOUSE
AccessioniPrimary (citable) accession number: Q80ZD8
Secondary accession number(s): A2AE40
, Q69ZQ0, Q8R5D4, Q921U9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 21, 2005
Last sequence update: June 1, 2003
Last modified: May 23, 2018
This is version 139 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families
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Main funding by: National Institutes of Health