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Protein

Amphoterin-induced protein 1

Gene

Amigo1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Promotes growth and fasciculation of neurites from cultured hippocampal neurons. May be involved in fasciculation as well as myelination of developing neural axons. May have a role in regeneration as well as neural plasticity in the adult nervous system. May mediate homophilic as well as heterophilic cell-cell interaction and contribute to signal transduction through its intracellular domain (PubMed:12629050). Assembled with KCNB1 modulates the gating characteristics of the delayed rectifier voltage-dependent potassium channel KCNB1 (By similarity).1 PublicationBy similarity1 Publication

GO - Molecular functioni

GO - Biological processi

  • axonal fasciculation Source: UniProtKB
  • axonogenesis Source: RGD
  • cell adhesion Source: RGD
  • heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules Source: UniProtKB
  • homophilic cell adhesion via plasma membrane adhesion molecules Source: UniProtKB
  • myelination Source: UniProtKB
  • positive regulation of axonogenesis Source: UniProtKB
  • positive regulation of potassium ion transmembrane transport Source: UniProtKB
  • positive regulation of synapse assembly Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Cell adhesion, Differentiation, Neurogenesis

Names & Taxonomyi

Protein namesi
Recommended name:
Amphoterin-induced protein 1
Alternative name(s):
AMIGO-1
Alivin-2
Gene namesi
Name:Amigo1By similarity
Synonyms:Ali2By similarity, Amigo1 Publication
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 2

Organism-specific databases

RGDi1303079. Amigo1.

Subcellular locationi

  • Cell membrane By similarity; Single-pass type I membrane protein By similarity
  • Perikaryon By similarity
  • Cell projectiondendrite By similarity
  • Cell projectionaxon 1 Publication

  • Note: Associated with axons of neuronal cells (PubMed:12629050). Colocalizes with KCNB1 at high-density somatodendritic clusters on the surface of hippocampal and cortical neurons (By similarity).By similarity

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini28 – 372ExtracellularSequence analysisAdd BLAST345
Transmembranei373 – 393HelicalSequence analysisAdd BLAST21
Topological domaini394 – 493CytoplasmicSequence analysisAdd BLAST100

GO - Cellular componenti

  • axon Source: UniProtKB-SubCell
  • dendrite Source: UniProtKB
  • integral component of membrane Source: RGD
  • integral component of plasma membrane Source: UniProtKB
  • neuronal cell body membrane Source: UniProtKB
  • perikaryon Source: UniProtKB-SubCell
  • voltage-gated potassium channel complex Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cell projection, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 27Sequence analysisAdd BLAST27
ChainiPRO_000001450828 – 493Amphoterin-induced protein 1Sequence analysisAdd BLAST466

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi34 ↔ 40PROSITE-ProRule annotation
Disulfide bondi38 ↔ 47PROSITE-ProRule annotation
Glycosylationi72N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi225 ↔ 253PROSITE-ProRule annotation
Disulfide bondi227 ↔ 270PROSITE-ProRule annotation
Glycosylationi269N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi290 ↔ 341PROSITE-ProRule annotation
Glycosylationi315N-linked (GlcNAc...)Sequence analysis1
Glycosylationi349N-linked (GlcNAc...)Sequence analysis1
Glycosylationi360N-linked (GlcNAc...)Sequence analysis1
Modified residuei477PhosphoserineCombined sources1
Modified residuei481PhosphoserineBy similarity1

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ80ZD7.
PRIDEiQ80ZD7.

PTM databases

iPTMnetiQ80ZD7.
PhosphoSitePlusiQ80ZD7.

Expressioni

Developmental stagei

Expressed at moderate levels in the central nervous system of stage 13-14 embryos. Highest levels at this stage in fiber tracts from dorsal root ganglia and trigeminal ganglion to the spinal chord as well as in fibers on both sides of the Purkinje cell layer of the cerebellum. Expression is down-regulated during postnatal stages P6 to P10 followed by up-regulation at the onset of myelination. High level expression in most myelinated axon tracts of the adult including cerebellum, pons, medulla, and spinal chord as well as in nonmyelinated fiber tracts in the striatum lucidum CA3 region of the hippocampus.1 Publication

Inductioni

By HMGB1/amphoterin; in cultured hippocampal neurons.1 Publication

Gene expression databases

BgeeiENSRNOG00000045665.
GenevisibleiQ80ZD7. RN.

Interactioni

Subunit structurei

Homodimer, and heterodimer with AMIGO2 and AMIGO3 (PubMed:12629050). Interacts with KCNB1 (By similarity).By similarity1 Publication

Protein-protein interaction databases

IntActiQ80ZD7. 1 interactor.
MINTiMINT-8298625.
STRINGi10116.ENSRNOP00000066608.

Structurei

3D structure databases

ProteinModelPortaliQ80ZD7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini28 – 61LRRNTAdd BLAST34
Repeati62 – 83LRR 1Add BLAST22
Repeati87 – 108LRR 2Add BLAST22
Repeati111 – 132LRR 3Add BLAST22
Repeati135 – 156LRR 4Add BLAST22
Repeati159 – 179LRR 5Add BLAST21
Repeati186 – 206LRR 6Add BLAST21
Domaini221 – 272LRRCTAdd BLAST52
Domaini269 – 353Ig-like C2-typeSequence analysisAdd BLAST85

Domaini

The LRR repeat region mediates homodimerization.By similarity

Sequence similaritiesi

Contains 6 LRR (leucine-rich) repeats.Sequence analysis
Contains 1 LRRCT domain.Curated
Contains 1 LRRNT domain.Curated

Keywords - Domaini

Immunoglobulin domain, Leucine-rich repeat, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IGN4. Eukaryota.
ENOG410ZZH4. LUCA.
GeneTreeiENSGT00530000063545.
HOGENOMiHOG000231327.
HOVERGENiHBG080231.
InParanoidiQ80ZD7.
OMAiCSEYKER.
OrthoDBiEOG091G06PG.
PhylomeDBiQ80ZD7.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
3.80.10.10. 1 hit.
InterProiIPR031283. AMIGO.
IPR031284. AMIGO1.
IPR000483. Cys-rich_flank_reg_C.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR013098. Ig_I-set.
IPR003599. Ig_sub.
IPR003598. Ig_sub2.
IPR032675. L_dom-like.
IPR001611. Leu-rich_rpt.
IPR003591. Leu-rich_rpt_typical-subtyp.
[Graphical view]
PANTHERiPTHR24368. PTHR24368. 1 hit.
PTHR24368:SF1. PTHR24368:SF1. 1 hit.
PfamiPF07679. I-set. 1 hit.
PF13855. LRR_8. 1 hit.
[Graphical view]
SMARTiSM00409. IG. 1 hit.
SM00408. IGc2. 1 hit.
SM00369. LRR_TYP. 5 hits.
SM00082. LRRCT. 1 hit.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 1 hit.
SSF52058. SSF52058. 1 hit.
PROSITEiPS50835. IG_LIKE. 1 hit.
PS51450. LRR. 6 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q80ZD7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQPQRDLRGL WLLLLSLFLL LFEVARAGRP VVSCPANCLC ASNILSCSKQ
60 70 80 90 100
QLPNVPQSLP GYTALLDLSH NNLSRLKAEW TPTRLTNLHS LLLSHNHLNF
110 120 130 140 150
ISSEAFVPVP NLRYLDLSSN HLHTLDEFLF SGLQALEVLL LYNNHIVVVD
160 170 180 190 200
RNAFEDMAQL QKLYLSQNMI SRFPLELIKD ANRLPKLTLL DLSSNKLKKL
210 220 230 240 250
PLTDLQKLPA WVKNGLYLHN NPLECDCKLY QLFSHWQYRQ LSSVMDFQED
260 270 280 290 300
LYCVHSKKLH NVFSLDFFNC SEYKESAWEA HLGDTLTITC DTKQQGMTKV
310 320 330 340 350
WVTPSNEQVL NQGANGTVTV SEDGNLHFKE VQVEDGGVYT CYAMGETFNE
360 370 380 390 400
TLSVELKVYN FTLHGHHDTL NTAYTTLVGC ILSVVLVLIY LYLTPCRCWC
410 420 430 440 450
RGVEKPSSHQ GDSLSSSMLS TTPNHDPMAG GDKDDGFDRR VAFLEPAGPG
460 470 480 490
QGQNGKLKPG NTLPVPEATG KGQRRMSDPE SVSSVFSDTP IVV
Length:493
Mass (Da):55,283
Last modified:June 1, 2003 - v1
Checksum:iF7FAAE936B2337D4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY237729 mRNA. Translation: AAO48950.1.
RefSeqiNP_996764.1. NM_206881.1.
XP_006233202.1. XM_006233140.2.
XP_008759613.1. XM_008761391.1.
UniGeneiRn.116338.

Genome annotation databases

EnsembliENSRNOT00000074725; ENSRNOP00000066608; ENSRNOG00000045665.
GeneIDi295365.
KEGGirno:295365.
UCSCiRGD:1303079. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY237729 mRNA. Translation: AAO48950.1.
RefSeqiNP_996764.1. NM_206881.1.
XP_006233202.1. XM_006233140.2.
XP_008759613.1. XM_008761391.1.
UniGeneiRn.116338.

3D structure databases

ProteinModelPortaliQ80ZD7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ80ZD7. 1 interactor.
MINTiMINT-8298625.
STRINGi10116.ENSRNOP00000066608.

PTM databases

iPTMnetiQ80ZD7.
PhosphoSitePlusiQ80ZD7.

Proteomic databases

PaxDbiQ80ZD7.
PRIDEiQ80ZD7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000074725; ENSRNOP00000066608; ENSRNOG00000045665.
GeneIDi295365.
KEGGirno:295365.
UCSCiRGD:1303079. rat.

Organism-specific databases

CTDi57463.
RGDi1303079. Amigo1.

Phylogenomic databases

eggNOGiENOG410IGN4. Eukaryota.
ENOG410ZZH4. LUCA.
GeneTreeiENSGT00530000063545.
HOGENOMiHOG000231327.
HOVERGENiHBG080231.
InParanoidiQ80ZD7.
OMAiCSEYKER.
OrthoDBiEOG091G06PG.
PhylomeDBiQ80ZD7.

Miscellaneous databases

PROiQ80ZD7.

Gene expression databases

BgeeiENSRNOG00000045665.
GenevisibleiQ80ZD7. RN.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
3.80.10.10. 1 hit.
InterProiIPR031283. AMIGO.
IPR031284. AMIGO1.
IPR000483. Cys-rich_flank_reg_C.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR013098. Ig_I-set.
IPR003599. Ig_sub.
IPR003598. Ig_sub2.
IPR032675. L_dom-like.
IPR001611. Leu-rich_rpt.
IPR003591. Leu-rich_rpt_typical-subtyp.
[Graphical view]
PANTHERiPTHR24368. PTHR24368. 1 hit.
PTHR24368:SF1. PTHR24368:SF1. 1 hit.
PfamiPF07679. I-set. 1 hit.
PF13855. LRR_8. 1 hit.
[Graphical view]
SMARTiSM00409. IG. 1 hit.
SM00408. IGc2. 1 hit.
SM00369. LRR_TYP. 5 hits.
SM00082. LRRCT. 1 hit.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 1 hit.
SSF52058. SSF52058. 1 hit.
PROSITEiPS50835. IG_LIKE. 1 hit.
PS51450. LRR. 6 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAMGO1_RAT
AccessioniPrimary (citable) accession number: Q80ZD7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 21, 2005
Last sequence update: June 1, 2003
Last modified: November 2, 2016
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.