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Protein

Sodium-driven chloride bicarbonate exchanger

Gene

Slc4a10

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Electrogenic sodium/bicarbonate cotransporter in exchange for intracellular chloride. Plays an important role in regulating intracellular pH.1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Antiport, Ion transport, Sodium transport, Symport, Transport

Keywords - Ligandi

Sodium

Names & Taxonomyi

Protein namesi
Recommended name:
Sodium-driven chloride bicarbonate exchanger
Alternative name(s):
Solute carrier family 4 member 10
Gene namesi
Name:Slc4a10
Synonyms:Ncbe
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi631407. Slc4a10.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 508508CytoplasmicSequence analysisAdd
BLAST
Transmembranei509 – 52921HelicalSequence analysisAdd
BLAST
Topological domaini530 – 5378ExtracellularSequence analysis
Transmembranei538 – 55821HelicalSequence analysisAdd
BLAST
Topological domaini559 – 5613CytoplasmicSequence analysis
Transmembranei562 – 58221HelicalSequence analysisAdd
BLAST
Topological domaini583 – 59513ExtracellularSequence analysisAdd
BLAST
Transmembranei596 – 61621HelicalSequence analysisAdd
BLAST
Topological domaini617 – 6259CytoplasmicSequence analysis
Transmembranei626 – 64621HelicalSequence analysisAdd
BLAST
Topological domaini647 – 71973ExtracellularSequence analysisAdd
BLAST
Transmembranei720 – 74021HelicalSequence analysisAdd
BLAST
Topological domaini741 – 76121CytoplasmicSequence analysisAdd
BLAST
Transmembranei762 – 78221HelicalSequence analysisAdd
BLAST
Topological domaini783 – 80826ExtracellularSequence analysisAdd
BLAST
Transmembranei809 – 82921HelicalSequence analysisAdd
BLAST
Topological domaini830 – 85425CytoplasmicSequence analysisAdd
BLAST
Transmembranei855 – 87521HelicalSequence analysisAdd
BLAST
Topological domaini876 – 91136ExtracellularSequence analysisAdd
BLAST
Transmembranei912 – 93221HelicalSequence analysisAdd
BLAST
Topological domaini933 – 9342CytoplasmicSequence analysis
Transmembranei935 – 95521HelicalSequence analysisAdd
BLAST
Topological domaini956 – 99742ExtracellularSequence analysisAdd
BLAST
Transmembranei998 – 101821HelicalSequence analysisAdd
BLAST
Topological domaini1019 – 111799CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11171117Sodium-driven chloride bicarbonate exchangerPRO_0000245242Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei89 – 891PhosphoserineCombined sources
Modified residuei94 – 941PhosphothreonineBy similarity
Modified residuei275 – 2751PhosphoserineBy similarity
Modified residuei1056 – 10561PhosphoserineBy similarity
Modified residuei1084 – 10841PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ80ZA5.
PRIDEiQ80ZA5.

Expressioni

Tissue specificityi

Expressed in the brain, in the hippocampus as well as in dentate gyrus, cortical layers, cerebellum, olfactory bulb and in the epithelial cells of the choroid plexus. Detected in pituitary, testis, kidney and ileum. Detected also in spleen and lung.3 Publications

Developmental stagei

Expressed in spinal cord and brain at E19 through adulthood.1 Publication

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
Slc9a3r1P704413EBI-8613086,EBI-1184085From a different organism.

Protein-protein interaction databases

IntActiQ80ZA5. 1 interaction.
STRINGi10116.ENSRNOP00000059155.

Structurei

3D structure databases

ProteinModelPortaliQ80ZA5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1172. Eukaryota.
ENOG410XPHD. LUCA.
HOVERGENiHBG004326.
InParanoidiQ80ZA5.
KOiK13861.
PhylomeDBiQ80ZA5.

Family and domain databases

Gene3Di3.40.1100.10. 2 hits.
InterProiIPR013769. Band3_cytoplasmic_dom.
IPR011531. HCO3_transpt_C.
IPR003020. HCO3_transpt_euk.
IPR003024. Na/HCO3_transpt.
IPR016152. PTrfase/Anion_transptr.
[Graphical view]
PANTHERiPTHR11453. PTHR11453. 1 hit.
PfamiPF07565. Band_3_cyto. 1 hit.
PF00955. HCO3_cotransp. 1 hit.
[Graphical view]
PRINTSiPR01231. HCO3TRNSPORT.
PR01232. NAHCO3TRSPRT.
SUPFAMiSSF55804. SSF55804. 2 hits.
TIGRFAMsiTIGR00834. ae. 1 hit.

Sequences (7)i

Sequence statusi: Complete.

This entry describes 7 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q80ZA5-1) [UniParc]FASTAAdd to basket

Also known as: rb1NCBE

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEIKDQGAQM EPLLPTRNDE EAVVDRGGTR SILKTHFEKE DLEGHRTLFI
60 70 80 90 100
GVHVPLGGRK SHRRHRHRGH KHRKRDRERD SGLEDGGESP SFDTPSQRVQ
110 120 130 140 150
FILGTEDDDE EHIPHDLFTE LDEICWREGE DAEWRETARW LKFEEDVEDG
160 170 180 190 200
GERWSKPYVA TLSLHSLFEL RSCILNGTVL LDMHANTLEE IADMVLDQQV
210 220 230 240 250
SSGQLNEDVR HRVHEALMKQ HHHQSQKKLT NRIPIVRSFA DIGKKQSEPN
260 270 280 290 300
SMDKNGQVVS PQSAPACAEN KNDVSRENST VDFSKGLGGQ QKGHTSPCGM
310 320 330 340 350
KQRLDKGPPH QQEREVDLHF MKKIPPGAEA SNILVGELEF LDRTVVAFVR
360 370 380 390 400
LSPAVLLQGL AEVPIPSRFL FILLGPLGKG QQYHEIGRSI ATLMTDEVFH
410 420 430 440 450
DVAYKAKDRN DLVSGIDEFL DQVTVLPPGE WDPSIRIEPP KNVPSQEKRK
460 470 480 490 500
TPSLPNGTAA HGGPEQHGGH SGPELQRTGR IFGGLILDIK RKAPFFWSDF
510 520 530 540 550
RDAFSLQCLA SFLFLYCACM SPVITFGGLL GEATEGRISA IESLFGASMT
560 570 580 590 600
GIAYSLFGGQ PLTILGSTGP VLVFEKILFK FCKEYGLSYL SLRASIGLWT
610 620 630 640 650
ATLCIILVAT DASSLVCYIT RFTEEAFASL ICIIFIYEAL EKLFELSESY
660 670 680 690 700
PINMHNDLEL LTQYSCNCME PHSPSNDTLK EWRESNISAS DIIWGNLTVS
710 720 730 740 750
ECRSLHGEYV GRACGHGHPY VPDVLFWSVI LFFSTVTMSA TLKQFKTSRY
760 770 780 790 800
FPTKVRSIVS DFAVFLTILC MVLIDYAIGI PSPKLQVPSV FKPTRDDRGW
810 820 830 840 850
FVTPLGPNPW WTIIAAIIPA LLCTILIFMD QQITAVIINR KEHKLKKGCG
860 870 880 890 900
YHLDLLMVAV MLGVCSIMGL PWFVAATVLS ITHVNSLKLE SECSAPGEQP
910 920 930 940 950
KFLGIREQRV TGLMIFILMG SSVFMTSILK FIPMPVLYGV FLYMGASSLK
960 970 980 990 1000
GIQLFDRIKL FWMPAKHQPD FIYLRHVPLR KVHLFTVIQM SCLGLLWIIK
1010 1020 1030 1040 1050
VSRAAIVFPM MVLALVFVRK LMDFLFTKRE LSWLDDLMPE SKKKKLEDAE
1060 1070 1080 1090 1100
KEEEQSMLAM EDEGTVQLPL EGHYRDDPSV INISDEMSKT AMWGNLLVTA
1110
DNSKEKESRF PSKSSPS
Length:1,117
Mass (Da):125,639
Last modified:June 1, 2003 - v1
Checksum:i51A27EFE653C3232
GO
Isoform 2 (identifier: Q80ZA5-2) [UniParc]FASTAAdd to basket

Also known as: rb4NCBE

The sequence of this isoform differs from the canonical sequence as follows:
     255-255: N → NA
     286-315: Missing.
     1115-1117: SPS → IESRKEKKADSGKGVDRETCL

Show »
Length:1,106
Mass (Da):124,503
Checksum:iCAB2CBDFB0876AC5
GO
Isoform 3 (identifier: Q80ZA5-3) [UniParc]FASTAAdd to basket

Also known as: rb2NCBE

The sequence of this isoform differs from the canonical sequence as follows:
     286-315: Missing.
     1115-1117: SPS → IESRKEKKADSGKGVDRETCL

Show »
Length:1,105
Mass (Da):124,432
Checksum:i50357AD41747F888
GO
Isoform 4 (identifier: Q80ZA5-4) [UniParc]FASTAAdd to basket

Also known as: rb5NCBE

The sequence of this isoform differs from the canonical sequence as follows:
     255-255: N → NA
     286-315: Missing.

Show »
Length:1,088
Mass (Da):122,442
Checksum:iF676A4A5382C6F94
GO
Isoform 5 (identifier: Q80ZA5-5) [UniParc]FASTAAdd to basket

Also known as: rb3NCBE

The sequence of this isoform differs from the canonical sequence as follows:
     255-255: N → NA
     1053-1053: E → H
     1054-1117: Missing.

Show »
Length:1,054
Mass (Da):118,620
Checksum:i2B28DC19A9796AC7
GO
Isoform 6 (identifier: Q80ZA5-6) [UniParc]FASTAAdd to basket

Also known as: rb6NCBE

The sequence of this isoform differs from the canonical sequence as follows:
     255-255: N → NA
     286-315: Missing.
     603-607: LCIIL → FLPLT
     608-1117: Missing.

Show »
Length:578
Mass (Da):64,626
Checksum:iB2CCECF7A9D82529
GO
Isoform 7 (identifier: Q80ZA5-7) [UniParc]FASTAAdd to basket

Also known as: rb7NCBE

The sequence of this isoform differs from the canonical sequence as follows:
     255-255: N → NA
     286-315: Missing.
     481-498: IFGGLILDIKRKAPFFWS → SPISGIFASPEFFHRLCG
     499-1117: Missing.

Show »
Length:469
Mass (Da):52,759
Checksum:iC527F48F7343A7E0
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei255 – 2551N → NA in isoform 2, isoform 4, isoform 5, isoform 6 and isoform 7. 1 PublicationVSP_019655
Alternative sequencei286 – 31530Missing in isoform 2, isoform 3, isoform 4, isoform 6 and isoform 7. 2 PublicationsVSP_019656Add
BLAST
Alternative sequencei481 – 49818IFGGL…PFFWS → SPISGIFASPEFFHRLCG in isoform 7. 1 PublicationVSP_019657Add
BLAST
Alternative sequencei499 – 1117619Missing in isoform 7. 1 PublicationVSP_019658Add
BLAST
Alternative sequencei603 – 6075LCIIL → FLPLT in isoform 6. 1 PublicationVSP_019659
Alternative sequencei608 – 1117510Missing in isoform 6. 1 PublicationVSP_019660Add
BLAST
Alternative sequencei1053 – 10531E → H in isoform 5. 1 PublicationVSP_019661
Alternative sequencei1054 – 111764Missing in isoform 5. 1 PublicationVSP_019662Add
BLAST
Alternative sequencei1115 – 11173SPS → IESRKEKKADSGKGVDRETC L in isoform 2 and isoform 3. 2 PublicationsVSP_019663

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF439855 mRNA. Translation: AAO59639.1.
AF439856 mRNA. Translation: AAO59640.1.
AY579373 mRNA. Translation: AAS89262.1.
AY579374 mRNA. Translation: AAS89263.1.
AY579375 mRNA. Translation: AAS89264.1.
AY579376 mRNA. Translation: AAS89265.1.
AY579377 mRNA. Translation: AAS89266.1.
RefSeqiNP_835193.1. NM_178092.2. [Q80ZA5-1]
XP_006234344.1. XM_006234282.2. [Q80ZA5-2]
XP_006234345.1. XM_006234283.2. [Q80ZA5-3]
XP_006234347.1. XM_006234285.2. [Q80ZA5-4]
UniGeneiRn.15193.
Rn.205129.

Genome annotation databases

GeneIDi295645.
KEGGirno:295645.
UCSCiRGD:631407. rat. [Q80ZA5-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF439855 mRNA. Translation: AAO59639.1.
AF439856 mRNA. Translation: AAO59640.1.
AY579373 mRNA. Translation: AAS89262.1.
AY579374 mRNA. Translation: AAS89263.1.
AY579375 mRNA. Translation: AAS89264.1.
AY579376 mRNA. Translation: AAS89265.1.
AY579377 mRNA. Translation: AAS89266.1.
RefSeqiNP_835193.1. NM_178092.2. [Q80ZA5-1]
XP_006234344.1. XM_006234282.2. [Q80ZA5-2]
XP_006234345.1. XM_006234283.2. [Q80ZA5-3]
XP_006234347.1. XM_006234285.2. [Q80ZA5-4]
UniGeneiRn.15193.
Rn.205129.

3D structure databases

ProteinModelPortaliQ80ZA5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ80ZA5. 1 interaction.
STRINGi10116.ENSRNOP00000059155.

Proteomic databases

PaxDbiQ80ZA5.
PRIDEiQ80ZA5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi295645.
KEGGirno:295645.
UCSCiRGD:631407. rat. [Q80ZA5-1]

Organism-specific databases

CTDi57282.
RGDi631407. Slc4a10.

Phylogenomic databases

eggNOGiKOG1172. Eukaryota.
ENOG410XPHD. LUCA.
HOVERGENiHBG004326.
InParanoidiQ80ZA5.
KOiK13861.
PhylomeDBiQ80ZA5.

Miscellaneous databases

NextBioi639819.
PROiQ80ZA5.

Family and domain databases

Gene3Di3.40.1100.10. 2 hits.
InterProiIPR013769. Band3_cytoplasmic_dom.
IPR011531. HCO3_transpt_C.
IPR003020. HCO3_transpt_euk.
IPR003024. Na/HCO3_transpt.
IPR016152. PTrfase/Anion_transptr.
[Graphical view]
PANTHERiPTHR11453. PTHR11453. 1 hit.
PfamiPF07565. Band_3_cyto. 1 hit.
PF00955. HCO3_cotransp. 1 hit.
[Graphical view]
PRINTSiPR01231. HCO3TRNSPORT.
PR01232. NAHCO3TRSPRT.
SUPFAMiSSF55804. SSF55804. 2 hits.
TIGRFAMsiTIGR00834. ae. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Two variants of the rat brain sodium-driven chloride bicarbonate exchanger (NCBE): developmental expression and addition of a PDZ motif."
    Giffard R.G., Lee Y.-S., Ouyang Y.-B., Murphy S.L., Monyer H.
    Eur. J. Neurosci. 18:2935-2945(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 3), TISSUE SPECIFICITY.
    Tissue: Brain.
  2. "Cloning and functional characterization of 'rb5NCBE'."
    Karim Z., Attmane-Elakeb A., Vernimmen C., Bichara M.M.
    Submitted (MAR-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 2; 4; 5; 6 AND 7).
    Strain: Sprague-Dawley.
    Tissue: Brain.
  3. "The Na+-driven Cl-/HCO3- exchanger. Cloning, tissue distribution, and functional characterization."
    Wang C.-Z., Yano H., Nagashima K., Seino S.
    J. Biol. Chem. 275:35486-35490(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY.
  4. "A SCL4A10 gene product maps selectively to the basolateral plasma membrane of choroid plexus epithelial cells."
    Praetorius J., Nejsum L.N., Nielsen S.
    Am. J. Physiol. 286:C601-C610(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, SUBCELLULAR LOCATION.
  5. "Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues."
    Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C., Olsen J.V.
    Nat. Commun. 3:876-876(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-89, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiS4A10_RAT
AccessioniPrimary (citable) accession number: Q80ZA5
Secondary accession number(s): Q6PU70
, Q6PU71, Q6PU72, Q6PU73, Q6PU74, Q80ZA6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 11, 2006
Last sequence update: June 1, 2003
Last modified: May 11, 2016
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.