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Q80Z39 (HCAR2_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 82. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Hydroxycarboxylic acid receptor 2
Alternative name(s):
G-protein coupled receptor 109
G-protein coupled receptor 109A
G-protein coupled receptor HM74
Niacin receptor 1
Nicotinic acid receptor
Protein PUMA-G
Gene names
Name:Hcar2
Synonyms:Gpr109, Gpr109a, Gpr109b, Niacr1, Pumag
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length360 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Acts as a high affinity receptor for both nicotinic acid (also known as niacin) and (D)-beta-hydroxybutyrate and mediates increased adiponectin secretion and decreased lipolysis through G(i)-protein-mediated inhibition of adenylyl cyclase. This pharmacological effect requires nicotinic acid doses that are much higher than those provided by a normal diet. Mediates nicotinic acid-induced apoptosis in mature neutrophils. Receptor activation by nicotinic acid results in reduced cAMP levels which may affect activity of cAMP-dependent protein kinase A and phosphorylation of target proteins, leading to neutrophil apoptosis. The rank order of potency for the displacement of nicotinic acid binding is 5-methyl pyrazole-3-carboxylic acid = pyridine-3-acetic acid > acifran > 5-methyl nicotinic acid = acipimox >> nicotinuric acid = nicotinamide. Ref.1 Ref.2 Ref.3

Subcellular location

Cell membrane; Multi-pass membrane protein.

Tissue specificity

Expressed in adipose tissue, lung and spleen. Ref.1 Ref.2

Sequence similarities

Belongs to the G-protein coupled receptor 1 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 360360Hydroxycarboxylic acid receptor 2
PRO_0000069606

Regions

Topological domain1 – 3030Extracellular Potential
Transmembrane31 – 5121Helical; Name=1; Potential
Topological domain52 – 609Cytoplasmic Potential
Transmembrane61 – 8121Helical; Name=2; Potential
Topological domain82 – 9817Extracellular Potential
Transmembrane99 – 11921Helical; Name=3; Potential
Topological domain120 – 14021Cytoplasmic Potential
Transmembrane141 – 16121Helical; Name=4; Potential
Topological domain162 – 18928Extracellular Potential
Transmembrane190 – 21021Helical; Name=5; Potential
Topological domain211 – 22616Cytoplasmic Potential
Transmembrane227 – 24721Helical; Name=6; Potential
Topological domain248 – 27023Extracellular Potential
Transmembrane271 – 29121Helical; Name=7; Potential
Topological domain292 – 36069Cytoplasmic Potential

Amino acid modifications

Disulfide bond97 ↔ 174 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q80Z39 [UniParc].

Last modified June 1, 2003. Version 1.
Checksum: 975BDEBCA448A6C5

FASTA36041,459
        10         20         30         40         50         60 
MSKQNHFLVI NGKNCCVFRD ENIAKVLPPV LGLEFVFGLL GNGLALWIFC FHLKSWKSSR 

        70         80         90        100        110        120 
IFLFNLAVAD FLLIICLPFL TDNYVQNWDW RFGSIPCRVM LFMLAMNRQG SIIFLTVVAV 

       130        140        150        160        170        180 
DRYFRVVHPH HFLNKISNRT AAIISCFLWG ITIGLTVHLL YTDMMTRNGD ANLCSSFSIC 

       190        200        210        220        230        240 
YTFRWHDAMF LLEFFLPLGI ILFCSGRIIW SLRQRQMDRH VKIKRAINFI MVVAIVFVIC 

       250        260        270        280        290        300 
FLPSVAVRIR IFWLLYKHNV RNCDIYSSVD LAFFTTLSFT YMNSMLDPVV YYFSSPSFPN 

       310        320        330        340        350        360 
FFSTCINRCL RRKTLGEPDN NRSTSVELTG DPSTIRSIPG ALMTDPSEPG SPPYLASTSR 

« Hide

References

[1]"Molecular identification of nicotinic acid receptor."
Soga T., Kamohara M., Takasaki J., Matsumoto S., Saito T., Ohishi T., Hiyama H., Matsuo A., Matsushime H., Furuichi K.
Biochem. Biophys. Res. Commun. 303:364-369(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY.
[2]"Molecular identification of high and low affinity receptors for nicotinic acid."
Wise A., Foord S.M., Fraser N.J., Barnes A.A., Elshourbagy N., Eilert M., Ignar D.M., Murdock P.R., Steplewski K., Green A., Brown A.J., Dowell S.J., Szekeres P.G., Hassall D.G., Marshall F.H., Wilson S., Pike N.B.
J. Biol. Chem. 278:9869-9874(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, TISSUE SPECIFICITY, CHARACTERIZATION.
[3]"Niacin stimulates adiponectin secretion through the GPR109A receptor."
Plaisance E.P., Lukasova M., Offermanns S., Zhang Y., Cao G., Judd R.L.
Am. J. Physiol. 296:E549-E558(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB103062 mRNA. Translation: BAC58009.1.
RefSeqNP_852141.1. NM_181476.1.
UniGeneRn.214478.

3D structure databases

ProteinModelPortalQ80Z39.
ModBaseSearch...
MobiDBSearch...

Chemistry

BindingDBQ80Z39.
ChEMBLCHEMBL4731.
GuidetoPHARMACOLOGY312.

Protein family/group databases

GPCRDBSearch...

PTM databases

PhosphoSiteQ80Z39.

Proteomic databases

PRIDEQ80Z39.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000032249; ENSRNOP00000036057; ENSRNOG00000026653.
GeneID353250.
KEGGrno:353250.
UCSCRGD:727952. rat.

Organism-specific databases

CTD80885.
RGD727952. Hcar2.

Phylogenomic databases

eggNOGNOG148880.
GeneTreeENSGT00510000046798.
InParanoidQ80Z39.
KOK08402.
OMARAINFIM.
OrthoDBEOG7R56SR.
PhylomeDBQ80Z39.
TreeFamTF330775.

Gene expression databases

GenevestigatorQ80Z39.

Family and domain databases

Gene3D1.20.1070.10. 1 hit.
InterProIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR028017. HCAR2/3_rcpt.
[Graphical view]
PANTHERPTHR24231:SF22. PTHR24231:SF22. 1 hit.
PfamPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSPR00237. GPCRRHODOPSN.
PROSITEPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio672627.
PROQ80Z39.

Entry information

Entry nameHCAR2_RAT
AccessionPrimary (citable) accession number: Q80Z39
Entry history
Integrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: June 1, 2003
Last modified: April 16, 2014
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

7-transmembrane G-linked receptors

List of 7-transmembrane G-linked receptor entries