Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Protein phosphatase 1E

Gene

Ppm1e

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Protein phosphatase that inactivates multifunctional CaM kinases such as CAMK4 and CAMK2. Dephosphorylates and inactivates PAK. May play a role in the inhibition of actin fiber stress breakdown and in morphological changes driven by TNK2/CDC42. Inactivates multifunctional CaM kinases. Dephosphorylates PRKAA2 (By similarity).By similarity

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Binds 2 magnesium or manganese ions per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi270 – 2701Manganese 1By similarity
Metal bindingi270 – 2701Manganese 2By similarity
Metal bindingi271 – 2711Manganese 1; via carbonyl oxygenBy similarity
Metal bindingi432 – 4321Manganese 2By similarity
Metal bindingi476 – 4761Manganese 2By similarity

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. protein serine/threonine phosphatase activity Source: UniProtKB

GO - Biological processi

  1. cellular response to drug Source: Ensembl
  2. negative regulation of protein kinase activity Source: Ensembl
  3. peptidyl-threonine dephosphorylation Source: Ensembl
  4. positive regulation of stress fiber assembly Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Ligandi

Magnesium, Manganese, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Protein phosphatase 1E (EC:3.1.3.16)
Alternative name(s):
Ca(2+)/calmodulin-dependent protein kinase phosphatase N
Short name:
CaMKP-N
CaMKP-nucleus
Short name:
CaMKN
Partner of PIX 1
Partner of PIX-alpha
Short name:
Partner of PIXA
Gene namesi
Name:Ppm1e
Synonyms:Camkn
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 10

Organism-specific databases

RGDi735028. Ppm1e.

Subcellular locationi

Nucleus. Cytoplasm
Note: A truncated form, major form, with the C-terminal part missing, is mostly found in the cytoplasm and a little in the nucleus throughout the central nervous system. Although in some large neurons, the full length, minor form, is found in the nucleus.

GO - Cellular componenti

  1. cytoplasm Source: RGD
  2. mitochondrion Source: RGD
  3. nucleolus Source: Ensembl
  4. nucleus Source: UniProtKB
  5. protein complex Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 750750Protein phosphatase 1EPRO_0000286822Add
BLAST

Proteomic databases

PaxDbiQ80Z30.
PRIDEiQ80Z30.

Expressioni

Tissue specificityi

Highly expressed in brain especially in hippocampus, thalamus and midbrain and to a lower extent in heart and spinal cord. Weakly expressed in testis. A very slow signal is also observed in heart, kidney and liver.2 Publications

Gene expression databases

GenevestigatoriQ80Z30.

Interactioni

Subunit structurei

Heterotrimer. Interacts with PAX1 and ARHGEF6 (or ARHGEF7) (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
AldoaP050652EBI-7473061,EBI-522118
GapdhP047975EBI-7473061,EBI-349219

Protein-protein interaction databases

IntActiQ80Z30. 4 interactions.
MINTiMINT-4601785.
STRINGi10116.ENSRNOP00000003859.

Structurei

3D structure databases

ProteinModelPortaliQ80Z30.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati31 – 3221
Repeati33 – 3422
Repeati35 – 3623
Repeati37 – 3824; approximate
Repeati39 – 4025
Repeati41 – 4226
Repeati43 – 4427
Domaini243 – 485243PP2C-likeAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni31 – 44147 X 2 AA tandem repeats of P-EAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi7 – 10296Glu-richAdd
BLAST
Compositional biasi112 – 12716Pro-richAdd
BLAST

Sequence similaritiesi

Belongs to the PP2C family.Curated
Contains 1 PP2C-like domain.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG0631.
GeneTreeiENSGT00740000114971.
HOGENOMiHOG000059266.
HOVERGENiHBG098260.
InParanoidiQ80Z30.
KOiK17501.
OMAiPVEMFGP.
OrthoDBiEOG7D2FDH.
PhylomeDBiQ80Z30.
TreeFamiTF317617.

Family and domain databases

Gene3Di3.60.40.10. 1 hit.
InterProiIPR001932. PP2C-like_dom.
IPR000222. PP2C_Mn2_Asp60_BS.
IPR015655. Protein_Pase_2C.
[Graphical view]
PANTHERiPTHR13832. PTHR13832. 1 hit.
PfamiPF00481. PP2C. 1 hit.
[Graphical view]
SMARTiSM00331. PP2C_SIG. 1 hit.
SM00332. PP2Cc. 1 hit.
[Graphical view]
SUPFAMiSSF81606. SSF81606. 1 hit.
PROSITEiPS01032. PP2C. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q80Z30-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGCIPEEKT YRRFLELFLG EFRGPCGGGE PEPEPESEPE PEPEAELVAA
60 70 80 90 100
EAAEASGEDP GEDAATVEAA EEGVQDQDPE PEEEAVEEEA AAEGEEEEEE
110 120 130 140 150
EEAAAPGHSA VPPPQPQLPP LPPLPRPLSE RITREEVEGE SLDLCLQQLY
160 170 180 190 200
KYNCPSFLAA ALARATSDEV LQSDLSAHCI PKETDGTEGT VEIETVKLAR
210 220 230 240 250
SVFSKLHEIC CNWVKDFPLR RRPQIYYETS IHAIKNMRRK MEDKHVCIPD
260 270 280 290 300
FNMLFNLEDQ EEQAYFAVFD GHGGVDAAIY ASVHLHVNLV RQEMFPHDPA
310 320 330 340 350
EALCRAFRVT DERFVQKAAR ESLRCGTTGV VTFIRGNMLH VAWVGDSQVM
360 370 380 390 400
LVRKGQAVEL MKPHKPDRED EKQRIEALGG CVVWFGAWRV NGSLSVSRAI
410 420 430 440 450
GDAEHKPYIC GDADSASTVL DGTEDYLILA CDGFYDTVNP DEAVKVVSDH
460 470 480 490 500
LKENNGDSSM VAHKLVASAR DAGSSDNITV IVVFLRDMNK AVNVSEESDW
510 520 530 540 550
TENSFQGGQE DGGDDKENHG ECKRPWPQHQ CSAPADLGYE GRVDSFTDRT
560 570 580 590 600
SLSPGPQINV LEDPDYLDLT QIETSKPHST QFLPPVEMIG PGAPKKAYVN
610 620 630 640 650
ELIMEESSVT PSQPERSGAG ELLVSFNLGS TGQQICRMEN LSPVYSGLEN
660 670 680 690 700
EQFKSPGKRA SRLYHLRHHY SKRQRGFRFN PKFYSFFSAQ ESSRKIGISL
710 720 730 740 750
SSLTRSGKRN KMLRSSLPWR ENSWEGYSGN MAIRKRNNIS CPDLPWDYKI
Length:750
Mass (Da):83,439
Last modified:June 1, 2003 - v1
Checksum:iAD18B60517D57347
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB081729 mRNA. Translation: BAC66021.1.
RefSeqiNP_942068.1. NM_198773.1.
UniGeneiRn.201345.

Genome annotation databases

EnsembliENSRNOT00000003859; ENSRNOP00000003859; ENSRNOG00000024730.
GeneIDi360593.
KEGGirno:360593.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB081729 mRNA. Translation: BAC66021.1.
RefSeqiNP_942068.1. NM_198773.1.
UniGeneiRn.201345.

3D structure databases

ProteinModelPortaliQ80Z30.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ80Z30. 4 interactions.
MINTiMINT-4601785.
STRINGi10116.ENSRNOP00000003859.

Proteomic databases

PaxDbiQ80Z30.
PRIDEiQ80Z30.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000003859; ENSRNOP00000003859; ENSRNOG00000024730.
GeneIDi360593.
KEGGirno:360593.

Organism-specific databases

CTDi22843.
RGDi735028. Ppm1e.

Phylogenomic databases

eggNOGiCOG0631.
GeneTreeiENSGT00740000114971.
HOGENOMiHOG000059266.
HOVERGENiHBG098260.
InParanoidiQ80Z30.
KOiK17501.
OMAiPVEMFGP.
OrthoDBiEOG7D2FDH.
PhylomeDBiQ80Z30.
TreeFamiTF317617.

Miscellaneous databases

NextBioi673375.
PROiQ80Z30.

Gene expression databases

GenevestigatoriQ80Z30.

Family and domain databases

Gene3Di3.60.40.10. 1 hit.
InterProiIPR001932. PP2C-like_dom.
IPR000222. PP2C_Mn2_Asp60_BS.
IPR015655. Protein_Pase_2C.
[Graphical view]
PANTHERiPTHR13832. PTHR13832. 1 hit.
PfamiPF00481. PP2C. 1 hit.
[Graphical view]
SMARTiSM00331. PP2C_SIG. 1 hit.
SM00332. PP2Cc. 1 hit.
[Graphical view]
SUPFAMiSSF81606. SSF81606. 1 hit.
PROSITEiPS01032. PP2C. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Subcellular distributions of rat CaM kinase phosphatase N and other members of the CaM kinase regulatory system."
    Kitani T., Okuno S., Takeuchi M., Fujisawa H.
    J. Neurochem. 86:77-85(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, TISSUE SPECIFICITY, FUNCTION.
    Tissue: Brain.
  2. "Identification and characterization of CaMKP-N, nuclear calmodulin-dependent protein kinase phosphatase."
    Takeuchi M., Ishida A., Kameshita I., Kitani T., Okuno S., Fujisawa H.
    J. Biochem. 130:833-840(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
  3. "Post-translational excision of the carboxyl-terminal segment of CaM kinase phosphatase N and its cytosolic occurrence in the brain."
    Kitani T., Okuno S., Nakamura Y., Tokuno H., Takeuchi M., Fujisawa H.
    J. Neurochem. 96:374-384(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.

Entry informationi

Entry nameiPPM1E_RAT
AccessioniPrimary (citable) accession number: Q80Z30
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 15, 2007
Last sequence update: June 1, 2003
Last modified: January 7, 2015
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.