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Q80Z30 (PPM1E_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 83. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protein phosphatase 1E

EC=3.1.3.16
Alternative name(s):
Ca(2+)/calmodulin-dependent protein kinase phosphatase N
Short name=CaMKP-N
CaMKP-nucleus
Short name=CaMKN
Partner of PIX 1
Partner of PIX-alpha
Short name=Partner of PIXA
Gene names
Name:Ppm1e
Synonyms:Camkn
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length750 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Protein phosphatase that inactivates multifunctional CaM kinases such as CAMK4 and CAMK2. Dephosphorylates and inactivates PAK. May play a role in the inhibition of actin fiber stress breakdown and in morphological changes driven by TNK2/CDC42. Inactivates multifunctional CaM kinases. Dephosphorylates PRKAA2 By similarity. Ref.1

Catalytic activity

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity.

Subunit structure

Heterotrimer. Interacts with PAX1 and ARHGEF6 (or ARHGEF7) By similarity.

Subcellular location

Nucleus. Cytoplasm. Note: A truncated form, major form, with the C-terminal part missing, is mostly found in the cytoplasm and a little in the nucleus throughout the central nervous system. Although in some large neurons, the full length, minor form, is found in the nucleus. Ref.1 Ref.2 Ref.3

Tissue specificity

Highly expressed in brain especially in hippocampus, thalamus and midbrain and to a lower extent in heart and spinal cord. Weakly expressed in testis. A very slow signal is also observed in heart, kidney and liver. Ref.1 Ref.2

Sequence similarities

Belongs to the PP2C family.

Contains 1 PP2C-like domain.

Binary interactions

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 750750Protein phosphatase 1E
PRO_0000286822

Regions

Repeat31 – 3221
Repeat33 – 3422
Repeat35 – 3623
Repeat37 – 3824; approximate
Repeat39 – 4025
Repeat41 – 4226
Repeat43 – 4427
Domain243 – 485243PP2C-like
Region31 – 44147 X 2 AA tandem repeats of P-E
Compositional bias7 – 10296Glu-rich
Compositional bias112 – 12716Pro-rich

Sites

Metal binding2701Manganese 1 By similarity
Metal binding2701Manganese 2 By similarity
Metal binding2711Manganese 1; via carbonyl oxygen By similarity
Metal binding4321Manganese 2 By similarity
Metal binding4761Manganese 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q80Z30 [UniParc].

Last modified June 1, 2003. Version 1.
Checksum: AD18B60517D57347

FASTA75083,439
        10         20         30         40         50         60 
MAGCIPEEKT YRRFLELFLG EFRGPCGGGE PEPEPESEPE PEPEAELVAA EAAEASGEDP 

        70         80         90        100        110        120 
GEDAATVEAA EEGVQDQDPE PEEEAVEEEA AAEGEEEEEE EEAAAPGHSA VPPPQPQLPP 

       130        140        150        160        170        180 
LPPLPRPLSE RITREEVEGE SLDLCLQQLY KYNCPSFLAA ALARATSDEV LQSDLSAHCI 

       190        200        210        220        230        240 
PKETDGTEGT VEIETVKLAR SVFSKLHEIC CNWVKDFPLR RRPQIYYETS IHAIKNMRRK 

       250        260        270        280        290        300 
MEDKHVCIPD FNMLFNLEDQ EEQAYFAVFD GHGGVDAAIY ASVHLHVNLV RQEMFPHDPA 

       310        320        330        340        350        360 
EALCRAFRVT DERFVQKAAR ESLRCGTTGV VTFIRGNMLH VAWVGDSQVM LVRKGQAVEL 

       370        380        390        400        410        420 
MKPHKPDRED EKQRIEALGG CVVWFGAWRV NGSLSVSRAI GDAEHKPYIC GDADSASTVL 

       430        440        450        460        470        480 
DGTEDYLILA CDGFYDTVNP DEAVKVVSDH LKENNGDSSM VAHKLVASAR DAGSSDNITV 

       490        500        510        520        530        540 
IVVFLRDMNK AVNVSEESDW TENSFQGGQE DGGDDKENHG ECKRPWPQHQ CSAPADLGYE 

       550        560        570        580        590        600 
GRVDSFTDRT SLSPGPQINV LEDPDYLDLT QIETSKPHST QFLPPVEMIG PGAPKKAYVN 

       610        620        630        640        650        660 
ELIMEESSVT PSQPERSGAG ELLVSFNLGS TGQQICRMEN LSPVYSGLEN EQFKSPGKRA 

       670        680        690        700        710        720 
SRLYHLRHHY SKRQRGFRFN PKFYSFFSAQ ESSRKIGISL SSLTRSGKRN KMLRSSLPWR 

       730        740        750 
ENSWEGYSGN MAIRKRNNIS CPDLPWDYKI 

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References

[1]"Subcellular distributions of rat CaM kinase phosphatase N and other members of the CaM kinase regulatory system."
Kitani T., Okuno S., Takeuchi M., Fujisawa H.
J. Neurochem. 86:77-85(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, TISSUE SPECIFICITY, FUNCTION.
Tissue: Brain.
[2]"Identification and characterization of CaMKP-N, nuclear calmodulin-dependent protein kinase phosphatase."
Takeuchi M., Ishida A., Kameshita I., Kitani T., Okuno S., Fujisawa H.
J. Biochem. 130:833-840(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[3]"Post-translational excision of the carboxyl-terminal segment of CaM kinase phosphatase N and its cytosolic occurrence in the brain."
Kitani T., Okuno S., Nakamura Y., Tokuno H., Takeuchi M., Fujisawa H.
J. Neurochem. 96:374-384(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB081729 mRNA. Translation: BAC66021.1.
RefSeqNP_942068.1. NM_198773.1.
UniGeneRn.201345.

3D structure databases

ProteinModelPortalQ80Z30.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ80Z30. 4 interactions.
MINTMINT-4601785.
STRING10116.ENSRNOP00000003859.

Proteomic databases

PaxDbQ80Z30.
PRIDEQ80Z30.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000003859; ENSRNOP00000003859; ENSRNOG00000024730.
GeneID360593.
KEGGrno:360593.

Organism-specific databases

CTD22843.
RGD735028. Ppm1e.

Phylogenomic databases

eggNOGCOG0631.
GeneTreeENSGT00740000114971.
HOGENOMHOG000059266.
HOVERGENHBG098260.
InParanoidQ80Z30.
KOK17501.
OMAPHSAQFL.
OrthoDBEOG7D2FDH.
PhylomeDBQ80Z30.
TreeFamTF317617.

Gene expression databases

GenevestigatorQ80Z30.

Family and domain databases

Gene3D3.60.40.10. 1 hit.
InterProIPR001932. PP2C-like_dom.
IPR000222. PP2C_Mn2_Asp60_BS.
IPR015655. Protein_Pase_2C.
[Graphical view]
PANTHERPTHR13832. PTHR13832. 1 hit.
PfamPF00481. PP2C. 1 hit.
[Graphical view]
SMARTSM00331. PP2C_SIG. 1 hit.
SM00332. PP2Cc. 1 hit.
[Graphical view]
SUPFAMSSF81606. SSF81606. 1 hit.
PROSITEPS01032. PP2C. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio673375.
PROQ80Z30.

Entry information

Entry namePPM1E_RAT
AccessionPrimary (citable) accession number: Q80Z30
Entry history
Integrated into UniProtKB/Swiss-Prot: May 15, 2007
Last sequence update: June 1, 2003
Last modified: April 16, 2014
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families