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Protein

Protein phosphatase 1E

Gene

Ppm1e

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Protein phosphatase that inactivates multifunctional CaM kinases such as CAMK4 and CAMK2. Dephosphorylates and inactivates PAK. May play a role in the inhibition of actin fiber stress breakdown and in morphological changes driven by TNK2/CDC42. Inactivates multifunctional CaM kinases. Dephosphorylates PRKAA2 (By similarity).By similarity1 Publication

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Binds 2 magnesium or manganese ions per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi270Manganese 1By similarity1
Metal bindingi270Manganese 2By similarity1
Metal bindingi271Manganese 1; via carbonyl oxygenBy similarity1
Metal bindingi432Manganese 2By similarity1
Metal bindingi476Manganese 2By similarity1

GO - Molecular functioni

  • metal ion binding Source: UniProtKB-KW
  • protein serine/threonine phosphatase activity Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Ligandi

Magnesium, Manganese, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Protein phosphatase 1E (EC:3.1.3.16)
Alternative name(s):
Ca(2+)/calmodulin-dependent protein kinase phosphatase N
Short name:
CaMKP-N
CaMKP-nucleus
Short name:
CaMKN
Partner of PIX 1
Partner of PIX-alpha
Short name:
Partner of PIXA
Gene namesi
Name:Ppm1e
Synonyms:Camkn
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 10

Organism-specific databases

RGDi735028. Ppm1e.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: RGD
  • mitochondrion Source: RGD
  • nucleolus Source: Ensembl
  • nucleus Source: UniProtKB
  • protein complex Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002868221 – 750Protein phosphatase 1EAdd BLAST750

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei532PhosphoserineBy similarity1
Modified residuei545PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ80Z30.
PRIDEiQ80Z30.

PTM databases

iPTMnetiQ80Z30.
PhosphoSitePlusiQ80Z30.

Expressioni

Tissue specificityi

Highly expressed in brain especially in hippocampus, thalamus and midbrain and to a lower extent in heart and spinal cord. Weakly expressed in testis. A very slow signal is also observed in heart, kidney and liver.2 Publications

Gene expression databases

BgeeiENSRNOG00000024730.

Interactioni

Subunit structurei

Heterotrimer. Interacts with PAX1 and ARHGEF6 (or ARHGEF7) (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
AldoaP050652EBI-7473061,EBI-522118
GapdhP047975EBI-7473061,EBI-349219

Protein-protein interaction databases

IntActiQ80Z30. 4 interactors.
MINTiMINT-4601785.
STRINGi10116.ENSRNOP00000003859.

Structurei

3D structure databases

ProteinModelPortaliQ80Z30.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati31 – 3212
Repeati33 – 3422
Repeati35 – 3632
Repeati37 – 384; approximate2
Repeati39 – 4052
Repeati41 – 4262
Repeati43 – 4472
Domaini224 – 485PPM-type phosphatasePROSITE-ProRule annotationAdd BLAST262

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni31 – 447 X 2 AA tandem repeats of P-EAdd BLAST14

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi7 – 102Glu-richAdd BLAST96
Compositional biasi112 – 127Pro-richAdd BLAST16

Sequence similaritiesi

Belongs to the PP2C family.Curated
Contains 1 PPM-type phosphatase domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0698. Eukaryota.
COG0631. LUCA.
GeneTreeiENSGT00840000129732.
HOGENOMiHOG000059266.
HOVERGENiHBG098260.
InParanoidiQ80Z30.
KOiK17501.
OMAiPVEMFGP.
OrthoDBiEOG091G08KG.
PhylomeDBiQ80Z30.
TreeFamiTF317617.

Family and domain databases

Gene3Di3.60.40.10. 1 hit.
InterProiIPR015655. PP2C.
IPR000222. PP2C_BS.
IPR001932. PPM-type_phosphatase_dom.
[Graphical view]
PANTHERiPTHR13832. PTHR13832. 1 hit.
PfamiPF00481. PP2C. 1 hit.
[Graphical view]
SMARTiSM00332. PP2Cc. 1 hit.
[Graphical view]
SUPFAMiSSF81606. SSF81606. 1 hit.
PROSITEiPS01032. PPM_1. 1 hit.
PS51746. PPM_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q80Z30-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGCIPEEKT YRRFLELFLG EFRGPCGGGE PEPEPESEPE PEPEAELVAA
60 70 80 90 100
EAAEASGEDP GEDAATVEAA EEGVQDQDPE PEEEAVEEEA AAEGEEEEEE
110 120 130 140 150
EEAAAPGHSA VPPPQPQLPP LPPLPRPLSE RITREEVEGE SLDLCLQQLY
160 170 180 190 200
KYNCPSFLAA ALARATSDEV LQSDLSAHCI PKETDGTEGT VEIETVKLAR
210 220 230 240 250
SVFSKLHEIC CNWVKDFPLR RRPQIYYETS IHAIKNMRRK MEDKHVCIPD
260 270 280 290 300
FNMLFNLEDQ EEQAYFAVFD GHGGVDAAIY ASVHLHVNLV RQEMFPHDPA
310 320 330 340 350
EALCRAFRVT DERFVQKAAR ESLRCGTTGV VTFIRGNMLH VAWVGDSQVM
360 370 380 390 400
LVRKGQAVEL MKPHKPDRED EKQRIEALGG CVVWFGAWRV NGSLSVSRAI
410 420 430 440 450
GDAEHKPYIC GDADSASTVL DGTEDYLILA CDGFYDTVNP DEAVKVVSDH
460 470 480 490 500
LKENNGDSSM VAHKLVASAR DAGSSDNITV IVVFLRDMNK AVNVSEESDW
510 520 530 540 550
TENSFQGGQE DGGDDKENHG ECKRPWPQHQ CSAPADLGYE GRVDSFTDRT
560 570 580 590 600
SLSPGPQINV LEDPDYLDLT QIETSKPHST QFLPPVEMIG PGAPKKAYVN
610 620 630 640 650
ELIMEESSVT PSQPERSGAG ELLVSFNLGS TGQQICRMEN LSPVYSGLEN
660 670 680 690 700
EQFKSPGKRA SRLYHLRHHY SKRQRGFRFN PKFYSFFSAQ ESSRKIGISL
710 720 730 740 750
SSLTRSGKRN KMLRSSLPWR ENSWEGYSGN MAIRKRNNIS CPDLPWDYKI
Length:750
Mass (Da):83,439
Last modified:June 1, 2003 - v1
Checksum:iAD18B60517D57347
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB081729 mRNA. Translation: BAC66021.1.
RefSeqiNP_942068.1. NM_198773.1.
UniGeneiRn.201345.

Genome annotation databases

EnsembliENSRNOT00000003859; ENSRNOP00000003859; ENSRNOG00000024730.
GeneIDi360593.
KEGGirno:360593.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB081729 mRNA. Translation: BAC66021.1.
RefSeqiNP_942068.1. NM_198773.1.
UniGeneiRn.201345.

3D structure databases

ProteinModelPortaliQ80Z30.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ80Z30. 4 interactors.
MINTiMINT-4601785.
STRINGi10116.ENSRNOP00000003859.

PTM databases

iPTMnetiQ80Z30.
PhosphoSitePlusiQ80Z30.

Proteomic databases

PaxDbiQ80Z30.
PRIDEiQ80Z30.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000003859; ENSRNOP00000003859; ENSRNOG00000024730.
GeneIDi360593.
KEGGirno:360593.

Organism-specific databases

CTDi22843.
RGDi735028. Ppm1e.

Phylogenomic databases

eggNOGiKOG0698. Eukaryota.
COG0631. LUCA.
GeneTreeiENSGT00840000129732.
HOGENOMiHOG000059266.
HOVERGENiHBG098260.
InParanoidiQ80Z30.
KOiK17501.
OMAiPVEMFGP.
OrthoDBiEOG091G08KG.
PhylomeDBiQ80Z30.
TreeFamiTF317617.

Miscellaneous databases

PROiQ80Z30.

Gene expression databases

BgeeiENSRNOG00000024730.

Family and domain databases

Gene3Di3.60.40.10. 1 hit.
InterProiIPR015655. PP2C.
IPR000222. PP2C_BS.
IPR001932. PPM-type_phosphatase_dom.
[Graphical view]
PANTHERiPTHR13832. PTHR13832. 1 hit.
PfamiPF00481. PP2C. 1 hit.
[Graphical view]
SMARTiSM00332. PP2Cc. 1 hit.
[Graphical view]
SUPFAMiSSF81606. SSF81606. 1 hit.
PROSITEiPS01032. PPM_1. 1 hit.
PS51746. PPM_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPPM1E_RAT
AccessioniPrimary (citable) accession number: Q80Z30
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 15, 2007
Last sequence update: June 1, 2003
Last modified: November 2, 2016
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.