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Q80Z30

- PPM1E_RAT

UniProt

Q80Z30 - PPM1E_RAT

Protein

Protein phosphatase 1E

Gene

Ppm1e

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 87 (01 Oct 2014)
      Sequence version 1 (01 Jun 2003)
      Previous versions | rss
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    Functioni

    Protein phosphatase that inactivates multifunctional CaM kinases such as CAMK4 and CAMK2. Dephosphorylates and inactivates PAK. May play a role in the inhibition of actin fiber stress breakdown and in morphological changes driven by TNK2/CDC42. Inactivates multifunctional CaM kinases. Dephosphorylates PRKAA2 By similarity.By similarity

    Catalytic activityi

    [a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

    Cofactori

    Binds 2 magnesium or manganese ions per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi270 – 2701Manganese 1By similarity
    Metal bindingi270 – 2701Manganese 2By similarity
    Metal bindingi271 – 2711Manganese 1; via carbonyl oxygenBy similarity
    Metal bindingi432 – 4321Manganese 2By similarity
    Metal bindingi476 – 4761Manganese 2By similarity

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. protein binding Source: IntAct
    3. protein serine/threonine phosphatase activity Source: UniProtKB

    GO - Biological processi

    1. cellular response to drug Source: Ensembl
    2. negative regulation of protein kinase activity Source: Ensembl
    3. peptidyl-threonine dephosphorylation Source: Ensembl
    4. positive regulation of stress fiber assembly Source: Ensembl

    Keywords - Molecular functioni

    Hydrolase, Protein phosphatase

    Keywords - Ligandi

    Magnesium, Manganese, Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein phosphatase 1E (EC:3.1.3.16)
    Alternative name(s):
    Ca(2+)/calmodulin-dependent protein kinase phosphatase N
    Short name:
    CaMKP-N
    CaMKP-nucleus
    Short name:
    CaMKN
    Partner of PIX 1
    Partner of PIX-alpha
    Short name:
    Partner of PIXA
    Gene namesi
    Name:Ppm1e
    Synonyms:Camkn
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 10

    Organism-specific databases

    RGDi735028. Ppm1e.

    Subcellular locationi

    Nucleus. Cytoplasm
    Note: A truncated form, major form, with the C-terminal part missing, is mostly found in the cytoplasm and a little in the nucleus throughout the central nervous system. Although in some large neurons, the full length, minor form, is found in the nucleus.

    GO - Cellular componenti

    1. cytoplasm Source: RGD
    2. mitochondrion Source: RGD
    3. nucleolus Source: Ensembl
    4. nucleus Source: UniProtKB
    5. protein complex Source: Ensembl

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 750750Protein phosphatase 1EPRO_0000286822Add
    BLAST

    Proteomic databases

    PaxDbiQ80Z30.
    PRIDEiQ80Z30.

    Expressioni

    Tissue specificityi

    Highly expressed in brain especially in hippocampus, thalamus and midbrain and to a lower extent in heart and spinal cord. Weakly expressed in testis. A very slow signal is also observed in heart, kidney and liver.2 Publications

    Gene expression databases

    GenevestigatoriQ80Z30.

    Interactioni

    Subunit structurei

    Heterotrimer. Interacts with PAX1 and ARHGEF6 (or ARHGEF7) By similarity.By similarity

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    AldoaP050652EBI-7473061,EBI-522118
    GapdhP047975EBI-7473061,EBI-349219

    Protein-protein interaction databases

    IntActiQ80Z30. 4 interactions.
    MINTiMINT-4601785.
    STRINGi10116.ENSRNOP00000003859.

    Structurei

    3D structure databases

    ProteinModelPortaliQ80Z30.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati31 – 3221
    Repeati33 – 3422
    Repeati35 – 3623
    Repeati37 – 3824; approximate
    Repeati39 – 4025
    Repeati41 – 4226
    Repeati43 – 4427
    Domaini243 – 485243PP2C-likeAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni31 – 44147 X 2 AA tandem repeats of P-EAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi7 – 10296Glu-richAdd
    BLAST
    Compositional biasi112 – 12716Pro-richAdd
    BLAST

    Sequence similaritiesi

    Belongs to the PP2C family.Curated
    Contains 1 PP2C-like domain.Curated

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiCOG0631.
    GeneTreeiENSGT00740000114971.
    HOGENOMiHOG000059266.
    HOVERGENiHBG098260.
    InParanoidiQ80Z30.
    KOiK17501.
    OMAiPVEMFGP.
    OrthoDBiEOG7D2FDH.
    PhylomeDBiQ80Z30.
    TreeFamiTF317617.

    Family and domain databases

    Gene3Di3.60.40.10. 1 hit.
    InterProiIPR001932. PP2C-like_dom.
    IPR000222. PP2C_Mn2_Asp60_BS.
    IPR015655. Protein_Pase_2C.
    [Graphical view]
    PANTHERiPTHR13832. PTHR13832. 1 hit.
    PfamiPF00481. PP2C. 1 hit.
    [Graphical view]
    SMARTiSM00331. PP2C_SIG. 1 hit.
    SM00332. PP2Cc. 1 hit.
    [Graphical view]
    SUPFAMiSSF81606. SSF81606. 1 hit.
    PROSITEiPS01032. PP2C. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q80Z30-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAGCIPEEKT YRRFLELFLG EFRGPCGGGE PEPEPESEPE PEPEAELVAA    50
    EAAEASGEDP GEDAATVEAA EEGVQDQDPE PEEEAVEEEA AAEGEEEEEE 100
    EEAAAPGHSA VPPPQPQLPP LPPLPRPLSE RITREEVEGE SLDLCLQQLY 150
    KYNCPSFLAA ALARATSDEV LQSDLSAHCI PKETDGTEGT VEIETVKLAR 200
    SVFSKLHEIC CNWVKDFPLR RRPQIYYETS IHAIKNMRRK MEDKHVCIPD 250
    FNMLFNLEDQ EEQAYFAVFD GHGGVDAAIY ASVHLHVNLV RQEMFPHDPA 300
    EALCRAFRVT DERFVQKAAR ESLRCGTTGV VTFIRGNMLH VAWVGDSQVM 350
    LVRKGQAVEL MKPHKPDRED EKQRIEALGG CVVWFGAWRV NGSLSVSRAI 400
    GDAEHKPYIC GDADSASTVL DGTEDYLILA CDGFYDTVNP DEAVKVVSDH 450
    LKENNGDSSM VAHKLVASAR DAGSSDNITV IVVFLRDMNK AVNVSEESDW 500
    TENSFQGGQE DGGDDKENHG ECKRPWPQHQ CSAPADLGYE GRVDSFTDRT 550
    SLSPGPQINV LEDPDYLDLT QIETSKPHST QFLPPVEMIG PGAPKKAYVN 600
    ELIMEESSVT PSQPERSGAG ELLVSFNLGS TGQQICRMEN LSPVYSGLEN 650
    EQFKSPGKRA SRLYHLRHHY SKRQRGFRFN PKFYSFFSAQ ESSRKIGISL 700
    SSLTRSGKRN KMLRSSLPWR ENSWEGYSGN MAIRKRNNIS CPDLPWDYKI 750
    Length:750
    Mass (Da):83,439
    Last modified:June 1, 2003 - v1
    Checksum:iAD18B60517D57347
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB081729 mRNA. Translation: BAC66021.1.
    RefSeqiNP_942068.1. NM_198773.1.
    UniGeneiRn.201345.

    Genome annotation databases

    EnsembliENSRNOT00000003859; ENSRNOP00000003859; ENSRNOG00000024730.
    GeneIDi360593.
    KEGGirno:360593.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB081729 mRNA. Translation: BAC66021.1 .
    RefSeqi NP_942068.1. NM_198773.1.
    UniGenei Rn.201345.

    3D structure databases

    ProteinModelPortali Q80Z30.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q80Z30. 4 interactions.
    MINTi MINT-4601785.
    STRINGi 10116.ENSRNOP00000003859.

    Proteomic databases

    PaxDbi Q80Z30.
    PRIDEi Q80Z30.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000003859 ; ENSRNOP00000003859 ; ENSRNOG00000024730 .
    GeneIDi 360593.
    KEGGi rno:360593.

    Organism-specific databases

    CTDi 22843.
    RGDi 735028. Ppm1e.

    Phylogenomic databases

    eggNOGi COG0631.
    GeneTreei ENSGT00740000114971.
    HOGENOMi HOG000059266.
    HOVERGENi HBG098260.
    InParanoidi Q80Z30.
    KOi K17501.
    OMAi PVEMFGP.
    OrthoDBi EOG7D2FDH.
    PhylomeDBi Q80Z30.
    TreeFami TF317617.

    Miscellaneous databases

    NextBioi 673375.
    PROi Q80Z30.

    Gene expression databases

    Genevestigatori Q80Z30.

    Family and domain databases

    Gene3Di 3.60.40.10. 1 hit.
    InterProi IPR001932. PP2C-like_dom.
    IPR000222. PP2C_Mn2_Asp60_BS.
    IPR015655. Protein_Pase_2C.
    [Graphical view ]
    PANTHERi PTHR13832. PTHR13832. 1 hit.
    Pfami PF00481. PP2C. 1 hit.
    [Graphical view ]
    SMARTi SM00331. PP2C_SIG. 1 hit.
    SM00332. PP2Cc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF81606. SSF81606. 1 hit.
    PROSITEi PS01032. PP2C. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Subcellular distributions of rat CaM kinase phosphatase N and other members of the CaM kinase regulatory system."
      Kitani T., Okuno S., Takeuchi M., Fujisawa H.
      J. Neurochem. 86:77-85(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, TISSUE SPECIFICITY, FUNCTION.
      Tissue: Brain.
    2. "Identification and characterization of CaMKP-N, nuclear calmodulin-dependent protein kinase phosphatase."
      Takeuchi M., Ishida A., Kameshita I., Kitani T., Okuno S., Fujisawa H.
      J. Biochem. 130:833-840(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    3. "Post-translational excision of the carboxyl-terminal segment of CaM kinase phosphatase N and its cytosolic occurrence in the brain."
      Kitani T., Okuno S., Nakamura Y., Tokuno H., Takeuchi M., Fujisawa H.
      J. Neurochem. 96:374-384(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION.

    Entry informationi

    Entry nameiPPM1E_RAT
    AccessioniPrimary (citable) accession number: Q80Z30
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 15, 2007
    Last sequence update: June 1, 2003
    Last modified: October 1, 2014
    This is version 87 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3