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Q80Z30

- PPM1E_RAT

UniProt

Q80Z30 - PPM1E_RAT

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Protein

Protein phosphatase 1E

Gene
Ppm1e, Camkn
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Protein phosphatase that inactivates multifunctional CaM kinases such as CAMK4 and CAMK2. Dephosphorylates and inactivates PAK. May play a role in the inhibition of actin fiber stress breakdown and in morphological changes driven by TNK2/CDC42. Inactivates multifunctional CaM kinases. Dephosphorylates PRKAA2 By similarity.1 Publication

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactori

Binds 2 magnesium or manganese ions per subunit By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi270 – 2701Manganese 1 By similarity
Metal bindingi270 – 2701Manganese 2 By similarity
Metal bindingi271 – 2711Manganese 1; via carbonyl oxygen By similarity
Metal bindingi432 – 4321Manganese 2 By similarity
Metal bindingi476 – 4761Manganese 2 By similarity

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. protein binding Source: IntAct
  3. protein serine/threonine phosphatase activity Source: UniProtKB

GO - Biological processi

  1. cellular response to drug Source: Ensembl
  2. negative regulation of protein kinase activity Source: Ensembl
  3. peptidyl-threonine dephosphorylation Source: Ensembl
  4. positive regulation of stress fiber assembly Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Ligandi

Magnesium, Manganese, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Protein phosphatase 1E (EC:3.1.3.16)
Alternative name(s):
Ca(2+)/calmodulin-dependent protein kinase phosphatase N
Short name:
CaMKP-N
CaMKP-nucleus
Short name:
CaMKN
Partner of PIX 1
Partner of PIX-alpha
Short name:
Partner of PIXA
Gene namesi
Name:Ppm1e
Synonyms:Camkn
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 10

Organism-specific databases

RGDi735028. Ppm1e.

Subcellular locationi

Nucleus. Cytoplasm
Note: A truncated form, major form, with the C-terminal part missing, is mostly found in the cytoplasm and a little in the nucleus throughout the central nervous system. Although in some large neurons, the full length, minor form, is found in the nucleus.3 Publications

GO - Cellular componenti

  1. cytoplasm Source: RGD
  2. mitochondrion Source: RGD
  3. nucleolus Source: Ensembl
  4. nucleus Source: UniProtKB
  5. protein complex Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 750750Protein phosphatase 1EPRO_0000286822Add
BLAST

Proteomic databases

PaxDbiQ80Z30.
PRIDEiQ80Z30.

Expressioni

Tissue specificityi

Highly expressed in brain especially in hippocampus, thalamus and midbrain and to a lower extent in heart and spinal cord. Weakly expressed in testis. A very slow signal is also observed in heart, kidney and liver.2 Publications

Gene expression databases

GenevestigatoriQ80Z30.

Interactioni

Subunit structurei

Heterotrimer. Interacts with PAX1 and ARHGEF6 (or ARHGEF7) By similarity.

Binary interactionsi

WithEntry#Exp.IntActNotes
AldoaP050652EBI-7473061,EBI-522118
GapdhP047975EBI-7473061,EBI-349219

Protein-protein interaction databases

IntActiQ80Z30. 4 interactions.
MINTiMINT-4601785.
STRINGi10116.ENSRNOP00000003859.

Structurei

3D structure databases

ProteinModelPortaliQ80Z30.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati31 – 3221
Repeati33 – 3422
Repeati35 – 3623
Repeati37 – 3824; approximate
Repeati39 – 4025
Repeati41 – 4226
Repeati43 – 4427
Domaini243 – 485243PP2C-likeAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni31 – 44147 X 2 AA tandem repeats of P-EAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi7 – 10296Glu-richAdd
BLAST
Compositional biasi112 – 12716Pro-richAdd
BLAST

Sequence similaritiesi

Belongs to the PP2C family.
Contains 1 PP2C-like domain.

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG0631.
GeneTreeiENSGT00740000114971.
HOGENOMiHOG000059266.
HOVERGENiHBG098260.
InParanoidiQ80Z30.
KOiK17501.
OMAiPVEMFGP.
OrthoDBiEOG7D2FDH.
PhylomeDBiQ80Z30.
TreeFamiTF317617.

Family and domain databases

Gene3Di3.60.40.10. 1 hit.
InterProiIPR001932. PP2C-like_dom.
IPR000222. PP2C_Mn2_Asp60_BS.
IPR015655. Protein_Pase_2C.
[Graphical view]
PANTHERiPTHR13832. PTHR13832. 1 hit.
PfamiPF00481. PP2C. 1 hit.
[Graphical view]
SMARTiSM00331. PP2C_SIG. 1 hit.
SM00332. PP2Cc. 1 hit.
[Graphical view]
SUPFAMiSSF81606. SSF81606. 1 hit.
PROSITEiPS01032. PP2C. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q80Z30-1 [UniParc]FASTAAdd to Basket

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MAGCIPEEKT YRRFLELFLG EFRGPCGGGE PEPEPESEPE PEPEAELVAA    50
EAAEASGEDP GEDAATVEAA EEGVQDQDPE PEEEAVEEEA AAEGEEEEEE 100
EEAAAPGHSA VPPPQPQLPP LPPLPRPLSE RITREEVEGE SLDLCLQQLY 150
KYNCPSFLAA ALARATSDEV LQSDLSAHCI PKETDGTEGT VEIETVKLAR 200
SVFSKLHEIC CNWVKDFPLR RRPQIYYETS IHAIKNMRRK MEDKHVCIPD 250
FNMLFNLEDQ EEQAYFAVFD GHGGVDAAIY ASVHLHVNLV RQEMFPHDPA 300
EALCRAFRVT DERFVQKAAR ESLRCGTTGV VTFIRGNMLH VAWVGDSQVM 350
LVRKGQAVEL MKPHKPDRED EKQRIEALGG CVVWFGAWRV NGSLSVSRAI 400
GDAEHKPYIC GDADSASTVL DGTEDYLILA CDGFYDTVNP DEAVKVVSDH 450
LKENNGDSSM VAHKLVASAR DAGSSDNITV IVVFLRDMNK AVNVSEESDW 500
TENSFQGGQE DGGDDKENHG ECKRPWPQHQ CSAPADLGYE GRVDSFTDRT 550
SLSPGPQINV LEDPDYLDLT QIETSKPHST QFLPPVEMIG PGAPKKAYVN 600
ELIMEESSVT PSQPERSGAG ELLVSFNLGS TGQQICRMEN LSPVYSGLEN 650
EQFKSPGKRA SRLYHLRHHY SKRQRGFRFN PKFYSFFSAQ ESSRKIGISL 700
SSLTRSGKRN KMLRSSLPWR ENSWEGYSGN MAIRKRNNIS CPDLPWDYKI 750
Length:750
Mass (Da):83,439
Last modified:June 1, 2003 - v1
Checksum:iAD18B60517D57347
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB081729 mRNA. Translation: BAC66021.1.
RefSeqiNP_942068.1. NM_198773.1.
UniGeneiRn.201345.

Genome annotation databases

EnsembliENSRNOT00000003859; ENSRNOP00000003859; ENSRNOG00000024730.
GeneIDi360593.
KEGGirno:360593.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB081729 mRNA. Translation: BAC66021.1 .
RefSeqi NP_942068.1. NM_198773.1.
UniGenei Rn.201345.

3D structure databases

ProteinModelPortali Q80Z30.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q80Z30. 4 interactions.
MINTi MINT-4601785.
STRINGi 10116.ENSRNOP00000003859.

Proteomic databases

PaxDbi Q80Z30.
PRIDEi Q80Z30.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000003859 ; ENSRNOP00000003859 ; ENSRNOG00000024730 .
GeneIDi 360593.
KEGGi rno:360593.

Organism-specific databases

CTDi 22843.
RGDi 735028. Ppm1e.

Phylogenomic databases

eggNOGi COG0631.
GeneTreei ENSGT00740000114971.
HOGENOMi HOG000059266.
HOVERGENi HBG098260.
InParanoidi Q80Z30.
KOi K17501.
OMAi PVEMFGP.
OrthoDBi EOG7D2FDH.
PhylomeDBi Q80Z30.
TreeFami TF317617.

Miscellaneous databases

NextBioi 673375.
PROi Q80Z30.

Gene expression databases

Genevestigatori Q80Z30.

Family and domain databases

Gene3Di 3.60.40.10. 1 hit.
InterProi IPR001932. PP2C-like_dom.
IPR000222. PP2C_Mn2_Asp60_BS.
IPR015655. Protein_Pase_2C.
[Graphical view ]
PANTHERi PTHR13832. PTHR13832. 1 hit.
Pfami PF00481. PP2C. 1 hit.
[Graphical view ]
SMARTi SM00331. PP2C_SIG. 1 hit.
SM00332. PP2Cc. 1 hit.
[Graphical view ]
SUPFAMi SSF81606. SSF81606. 1 hit.
PROSITEi PS01032. PP2C. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Subcellular distributions of rat CaM kinase phosphatase N and other members of the CaM kinase regulatory system."
    Kitani T., Okuno S., Takeuchi M., Fujisawa H.
    J. Neurochem. 86:77-85(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, TISSUE SPECIFICITY, FUNCTION.
    Tissue: Brain.
  2. "Identification and characterization of CaMKP-N, nuclear calmodulin-dependent protein kinase phosphatase."
    Takeuchi M., Ishida A., Kameshita I., Kitani T., Okuno S., Fujisawa H.
    J. Biochem. 130:833-840(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
  3. "Post-translational excision of the carboxyl-terminal segment of CaM kinase phosphatase N and its cytosolic occurrence in the brain."
    Kitani T., Okuno S., Nakamura Y., Tokuno H., Takeuchi M., Fujisawa H.
    J. Neurochem. 96:374-384(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.

Entry informationi

Entry nameiPPM1E_RAT
AccessioniPrimary (citable) accession number: Q80Z30
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 15, 2007
Last sequence update: June 1, 2003
Last modified: July 9, 2014
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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