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Q80Z29

- NAMPT_RAT

UniProt

Q80Z29 - NAMPT_RAT

Protein

Nicotinamide phosphoribosyltransferase

Gene

Nampt

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 89 (01 Oct 2014)
      Sequence version 1 (01 Jun 2003)
      Previous versions | rss
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    Functioni

    Catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, an intermediate in the biosynthesis of NAD. It is the rate limiting component in the mammalian NAD biosynthesis pathway. The secreted form behaves both as a cytokine with immunomodulating properties and an adipokine with anti-diabetic properties, it has no enzymatic activity, partly because of lack of activation by ATP, which has a low level in extracellular space and plasma. Plays a role in the modulation of circadian clock function By similarity.By similarity

    Catalytic activityi

    Nicotinamide D-ribonucleotide + diphosphate = nicotinamide + 5-phospho-alpha-D-ribose 1-diphosphate.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei196 – 1961DiphosphateBy similarity
    Binding sitei219 – 2191Nicotinamide ribonucleotideBy similarity
    Binding sitei247 – 2471DiphosphateBy similarity
    Binding sitei311 – 3111DiphosphateBy similarity
    Binding sitei384 – 3841Nicotinamide ribonucleotide; via amide nitrogen
    Binding sitei392 – 3921Nicotinamide ribonucleotide

    GO - Molecular functioni

    1. cytokine activity Source: RGD
    2. drug binding Source: RGD
    3. nicotinamide phosphoribosyltransferase activity Source: RGD
    4. nicotinate-nucleotide diphosphorylase (carboxylating) activity Source: InterPro
    5. protein homodimerization activity Source: RGD

    GO - Biological processi

    1. female pregnancy Source: RGD
    2. NAD biosynthetic process Source: UniProtKB-UniPathway
    3. positive regulation of smooth muscle cell proliferation Source: RGD
    4. response to organic cyclic compound Source: RGD

    Keywords - Molecular functioni

    Cytokine, Glycosyltransferase, Transferase

    Keywords - Biological processi

    Pyridine nucleotide biosynthesis

    Enzyme and pathway databases

    BRENDAi2.4.2.12. 5301.
    ReactomeiREACT_196405. BMAL1:CLOCK,NPAS2 activates circadian gene expression.
    SABIO-RKQ80Z29.
    UniPathwayiUPA00253; UER00890.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Nicotinamide phosphoribosyltransferase (EC:2.4.2.12)
    Short name:
    NAmPRTase
    Short name:
    Nampt
    Alternative name(s):
    Pre-B-cell colony-enhancing factor 1 homolog
    Short name:
    PBEF
    Visfatin
    Gene namesi
    Name:Nampt
    Synonyms:Pbef1
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 6

    Organism-specific databases

    RGDi631365. Nampt.

    Subcellular locationi

    Nucleus 1 Publication. Cytoplasm 1 Publication. Secreted By similarity
    Note: Under non-inflammatory conditions, visfatin predominantly exhibits a granular pattern within the nucleus. Secreted by endothelial cells upon IL-1beta stimulation. Abundantly secreted in milk, reaching 100-fold higher concentrations compared to maternal serum By similarity.By similarity

    GO - Cellular componenti

    1. cytoplasm Source: RGD
    2. extracellular space Source: UniProtKB-KW
    3. nucleus Source: RGD

    Keywords - Cellular componenti

    Cytoplasm, Nucleus, Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 491491Nicotinamide phosphoribosyltransferasePRO_0000205866Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1 – 11N-acetylmethionineBy similarity

    Keywords - PTMi

    Acetylation

    Proteomic databases

    PaxDbiQ80Z29.
    PRIDEiQ80Z29.

    PTM databases

    PhosphoSiteiQ80Z29.

    Expressioni

    Tissue specificityi

    Expressed in various tissues. At the highest level in liver and at the second highest in heart. The amount is higher in heart than in lung.1 Publication

    Gene expression databases

    GenevestigatoriQ80Z29.

    Interactioni

    Subunit structurei

    Homodimer.1 Publication

    Structurei

    Secondary structure

    1
    491
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi11 – 133
    Beta strandi14 – 163
    Helixi17 – 248
    Beta strandi30 – 3910
    Beta strandi56 – 583
    Helixi62 – 698
    Beta strandi71 – 733
    Helixi77 – 9115
    Helixi98 – 10811
    Beta strandi114 – 1185
    Beta strandi124 – 13815
    Helixi139 – 1435
    Helixi144 – 1474
    Helixi149 – 1535
    Helixi156 – 18025
    Helixi186 – 1883
    Beta strandi189 – 1924
    Helixi195 – 1973
    Beta strandi198 – 2003
    Helixi201 – 21111
    Turni212 – 2143
    Beta strandi217 – 2193
    Helixi222 – 2309
    Beta strandi234 – 2363
    Beta strandi238 – 2403
    Helixi247 – 2504
    Helixi251 – 2533
    Helixi255 – 2573
    Helixi258 – 26811
    Beta strandi270 – 2723
    Beta strandi274 – 2785
    Helixi283 – 2886
    Helixi289 – 2946
    Helixi296 – 2994
    Beta strandi308 – 3114
    Helixi317 – 33115
    Beta strandi348 – 3525
    Helixi358 – 37013
    Helixi375 – 3773
    Beta strandi378 – 3836
    Helixi384 – 3874
    Helixi392 – 3943
    Beta strandi397 – 40610
    Beta strandi409 – 4113
    Helixi421 – 4233
    Beta strandi431 – 4344
    Beta strandi440 – 4434
    Helixi447 – 4504
    Beta strandi453 – 4553
    Beta strandi459 – 4635
    Helixi473 – 4797

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2G95X-ray1.90A/B1-491[»]
    2G96X-ray2.90A/B1-491[»]
    2G97X-ray2.90A/B1-491[»]
    3G8EX-ray3.00A/B1-491[»]
    ProteinModelPortaliQ80Z29.
    SMRiQ80Z29. Positions 9-483.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ80Z29.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni311 – 3133Nicotinamide ribonucleotide binding
    Regioni353 – 3542Nicotinamide ribonucleotide binding

    Sequence similaritiesi

    Belongs to the NAPRTase family.Curated

    Phylogenomic databases

    eggNOGiCOG1488.
    GeneTreeiENSGT00390000006647.
    HOGENOMiHOG000216546.
    HOVERGENiHBG000336.
    KOiK03462.
    OMAiKKFPITE.
    OrthoDBiEOG7PGDQH.

    Family and domain databases

    InterProiIPR007229. Nic_PRibTrfase-Fam.
    IPR016471. Nicotinamide_PRibTrfase.
    IPR002638. Quinolinate_PRibosylTrfase_C.
    [Graphical view]
    PANTHERiPTHR11098. PTHR11098. 1 hit.
    PTHR11098:SF2. PTHR11098:SF2. 1 hit.
    PfamiPF04095. NAPRTase. 1 hit.
    [Graphical view]
    PIRSFiPIRSF005943. NMPRT. 1 hit.
    SUPFAMiSSF51690. SSF51690. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q80Z29-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNAAAEAEFN ILLATDSYKV THYKQYPPNT SKVYSYFECR EKKTENSKVR    50
    KVKYEETVFY GLQYILNKYL KGKVVTKEKI QEAKEVYREH FQDDVFNERG 100
    WNYILEKYDG HLPIEVKAVP EGSVIPRGNV LFTVENTDPE CYWLTNWIET 150
    ILVQSWYPIT VATNSREQKK ILAKYLLETS GNLDGLEYKL HDFGYRGVSS 200
    QETAGIGASA HLVNFKGTDT VAGIALIKKY YGTKDPVPGY SVPAAEHSTI 250
    TAWGKDHEKD AFEHIVTQFS SVPVSVVSDS YDIYNACEKI WGEDLRHLIV 300
    SRSTEAPLII RPDSGNPLDT VLKVLDILGK KFPVSENSKG YKLLPPYLRV 350
    IQGDGVDINT LQEIVEGMKQ KKWSIENVSF GSGGALLQKL TRDLLNCSFK 400
    CSYVVTNGLG VNVFKDPVAD PNKRSKKGRL SLHRTPAGTF VTLEEGKGDL 450
    EEYGHDLLHT VFKNGKVTKS YSFDEVRKNA QLNMEQDVAP H 491
    Length:491
    Mass (Da):55,438
    Last modified:June 1, 2003 - v1
    Checksum:i78F526F0D3F579FC
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB081730 mRNA. Translation: BAC66022.1.
    AY831728 mRNA. Translation: AAX49410.1.
    BC085681 mRNA. Translation: AAH85681.1.
    RefSeqiNP_808789.1. NM_177928.3.
    UniGeneiRn.203508.

    Genome annotation databases

    EnsembliENSRNOT00000013043; ENSRNOP00000013043; ENSRNOG00000009754.
    GeneIDi297508.
    KEGGirno:297508.
    UCSCiRGD:631365. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB081730 mRNA. Translation: BAC66022.1 .
    AY831728 mRNA. Translation: AAX49410.1 .
    BC085681 mRNA. Translation: AAH85681.1 .
    RefSeqi NP_808789.1. NM_177928.3.
    UniGenei Rn.203508.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2G95 X-ray 1.90 A/B 1-491 [» ]
    2G96 X-ray 2.90 A/B 1-491 [» ]
    2G97 X-ray 2.90 A/B 1-491 [» ]
    3G8E X-ray 3.00 A/B 1-491 [» ]
    ProteinModelPortali Q80Z29.
    SMRi Q80Z29. Positions 9-483.
    ModBasei Search...
    MobiDBi Search...

    PTM databases

    PhosphoSitei Q80Z29.

    Proteomic databases

    PaxDbi Q80Z29.
    PRIDEi Q80Z29.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000013043 ; ENSRNOP00000013043 ; ENSRNOG00000009754 .
    GeneIDi 297508.
    KEGGi rno:297508.
    UCSCi RGD:631365. rat.

    Organism-specific databases

    CTDi 10135.
    RGDi 631365. Nampt.

    Phylogenomic databases

    eggNOGi COG1488.
    GeneTreei ENSGT00390000006647.
    HOGENOMi HOG000216546.
    HOVERGENi HBG000336.
    KOi K03462.
    OMAi KKFPITE.
    OrthoDBi EOG7PGDQH.

    Enzyme and pathway databases

    UniPathwayi UPA00253 ; UER00890 .
    BRENDAi 2.4.2.12. 5301.
    Reactomei REACT_196405. BMAL1:CLOCK,NPAS2 activates circadian gene expression.
    SABIO-RK Q80Z29.

    Miscellaneous databases

    EvolutionaryTracei Q80Z29.
    NextBioi 642340.
    PROi Q80Z29.

    Gene expression databases

    Genevestigatori Q80Z29.

    Family and domain databases

    InterProi IPR007229. Nic_PRibTrfase-Fam.
    IPR016471. Nicotinamide_PRibTrfase.
    IPR002638. Quinolinate_PRibosylTrfase_C.
    [Graphical view ]
    PANTHERi PTHR11098. PTHR11098. 1 hit.
    PTHR11098:SF2. PTHR11098:SF2. 1 hit.
    Pfami PF04095. NAPRTase. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF005943. NMPRT. 1 hit.
    SUPFAMi SSF51690. SSF51690. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Growth phase-dependent changes in the subcellular localization of pre-B-cell colony-enhancing factor."
      Kitani T., Okuno S., Fujisawa H.
      FEBS Lett. 544:74-78(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    2. "NAD biosynthesis pathway mediated by nicotinamide phosphoribosyltransferase attenuates ischemic neuronal cell death."
      Xing J., Gao Y., Cao G., Chen J.
      Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: Sprague-Dawley.
      Tissue: Brain.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Heart.
    4. "Crystal structure of visfatin/pre-B cell colony-enhancing factor 1/nicotinamide phosphoribosyltransferase, free and in complex with the anti-cancer agent FK-866."
      Kim M.-K., Lee J.H., Kim H., Park S.J., Kim S.H., Kang G.B., Lee Y.S., Kim J.B., Kim K.K., Suh S.W., Eom S.H.
      J. Mol. Biol. 362:66-77(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS) IN COMPLEXES WITH NICOTINAMIDE MONONUCLEOTIDE AND FK866, SUBUNIT.

    Entry informationi

    Entry nameiNAMPT_RAT
    AccessioniPrimary (citable) accession number: Q80Z29
    Secondary accession number(s): Q2VT47
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 5, 2005
    Last sequence update: June 1, 2003
    Last modified: October 1, 2014
    This is version 89 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3