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Q80Z29

- NAMPT_RAT

UniProt

Q80Z29 - NAMPT_RAT

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Protein

Nicotinamide phosphoribosyltransferase

Gene

Nampt

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, an intermediate in the biosynthesis of NAD. It is the rate limiting component in the mammalian NAD biosynthesis pathway. The secreted form behaves both as a cytokine with immunomodulating properties and an adipokine with anti-diabetic properties, it has no enzymatic activity, partly because of lack of activation by ATP, which has a low level in extracellular space and plasma. Plays a role in the modulation of circadian clock function. NAMPT-dependent oscillatory production of NAD regulates oscillation of clock target gene expression by releasing the core clock component: CLOCK-ARNTL/BMAL1 heterodimer from NAD-dependent SIRT1-mediated suppression.By similarity

Catalytic activityi

Nicotinamide D-ribonucleotide + diphosphate = nicotinamide + 5-phospho-alpha-D-ribose 1-diphosphate.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei196 – 1961DiphosphateBy similarity
Binding sitei219 – 2191Nicotinamide ribonucleotideBy similarity
Binding sitei247 – 2471DiphosphateBy similarity
Binding sitei311 – 3111DiphosphateBy similarity
Binding sitei384 – 3841Nicotinamide ribonucleotide; via amide nitrogen
Binding sitei392 – 3921Nicotinamide ribonucleotide

GO - Molecular functioni

  1. cytokine activity Source: RGD
  2. drug binding Source: RGD
  3. nicotinamide phosphoribosyltransferase activity Source: RGD
  4. nicotinate-nucleotide diphosphorylase (carboxylating) activity Source: InterPro
  5. protein homodimerization activity Source: RGD

GO - Biological processi

  1. circadian regulation of gene expression Source: UniProtKB
  2. female pregnancy Source: RGD
  3. NAD biosynthetic process Source: UniProtKB-UniPathway
  4. positive regulation of smooth muscle cell proliferation Source: RGD
  5. response to organic cyclic compound Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Cytokine, Glycosyltransferase, Transferase

Keywords - Biological processi

Biological rhythms, Pyridine nucleotide biosynthesis

Enzyme and pathway databases

BRENDAi2.4.2.12. 5301.
ReactomeiREACT_196405. BMAL1:CLOCK,NPAS2 activates circadian gene expression.
SABIO-RKQ80Z29.
UniPathwayiUPA00253; UER00890.

Names & Taxonomyi

Protein namesi
Recommended name:
Nicotinamide phosphoribosyltransferase (EC:2.4.2.12)
Short name:
NAmPRTase
Short name:
Nampt
Alternative name(s):
Pre-B-cell colony-enhancing factor 1 homolog
Short name:
PBEF
Visfatin
Gene namesi
Name:Nampt
Synonyms:Pbef1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 6

Organism-specific databases

RGDi631365. Nampt.

Subcellular locationi

Nucleus 1 Publication. Cytoplasm 1 Publication. Secreted By similarity
Note: Under non-inflammatory conditions, visfatin predominantly exhibits a granular pattern within the nucleus. Secreted by endothelial cells upon IL-1beta stimulation. Abundantly secreted in milk, reaching 100-fold higher concentrations compared to maternal serum.By similarity

GO - Cellular componenti

  1. cytoplasm Source: RGD
  2. extracellular space Source: UniProtKB-KW
  3. nucleus Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 491491Nicotinamide phosphoribosyltransferasePRO_0000205866Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiQ80Z29.
PRIDEiQ80Z29.

PTM databases

PhosphoSiteiQ80Z29.

Expressioni

Tissue specificityi

Expressed in various tissues. At the highest level in liver and at the second highest in heart. The amount is higher in heart than in lung.1 Publication

Gene expression databases

GenevestigatoriQ80Z29.

Interactioni

Subunit structurei

Homodimer.1 Publication

Structurei

Secondary structure

1
491
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi11 – 133
Beta strandi14 – 163
Helixi17 – 248
Beta strandi30 – 3910
Beta strandi56 – 583
Helixi62 – 698
Beta strandi71 – 733
Helixi77 – 9115
Helixi98 – 10811
Beta strandi114 – 1185
Beta strandi124 – 13815
Helixi139 – 1435
Helixi144 – 1474
Helixi149 – 1535
Helixi156 – 18025
Helixi186 – 1883
Beta strandi189 – 1924
Helixi195 – 1973
Beta strandi198 – 2003
Helixi201 – 21111
Turni212 – 2143
Beta strandi217 – 2193
Helixi222 – 2309
Beta strandi234 – 2363
Beta strandi238 – 2403
Helixi247 – 2504
Helixi251 – 2533
Helixi255 – 2573
Helixi258 – 26811
Beta strandi270 – 2723
Beta strandi274 – 2785
Helixi283 – 2886
Helixi289 – 2946
Helixi296 – 2994
Beta strandi308 – 3114
Helixi317 – 33115
Beta strandi348 – 3525
Helixi358 – 37013
Helixi375 – 3773
Beta strandi378 – 3836
Helixi384 – 3874
Helixi392 – 3943
Beta strandi397 – 40610
Beta strandi409 – 4113
Helixi421 – 4233
Beta strandi431 – 4344
Beta strandi440 – 4434
Helixi447 – 4504
Beta strandi453 – 4553
Beta strandi459 – 4635
Helixi473 – 4797

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2G95X-ray1.90A/B1-491[»]
2G96X-ray2.90A/B1-491[»]
2G97X-ray2.90A/B1-491[»]
3G8EX-ray3.00A/B1-491[»]
ProteinModelPortaliQ80Z29.
SMRiQ80Z29. Positions 9-483.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ80Z29.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni311 – 3133Nicotinamide ribonucleotide binding
Regioni353 – 3542Nicotinamide ribonucleotide binding

Sequence similaritiesi

Belongs to the NAPRTase family.Curated

Phylogenomic databases

eggNOGiCOG1488.
GeneTreeiENSGT00390000006647.
HOGENOMiHOG000216546.
HOVERGENiHBG000336.
InParanoidiQ80Z29.
KOiK03462.
OMAiKKFPITE.
OrthoDBiEOG7PGDQH.

Family and domain databases

InterProiIPR007229. Nic_PRibTrfase-Fam.
IPR016471. Nicotinamide_PRibTrfase.
IPR002638. Quinolinate_PRibosylTrfase_C.
[Graphical view]
PANTHERiPTHR11098. PTHR11098. 1 hit.
PTHR11098:SF2. PTHR11098:SF2. 1 hit.
PfamiPF04095. NAPRTase. 1 hit.
[Graphical view]
PIRSFiPIRSF005943. NMPRT. 1 hit.
SUPFAMiSSF51690. SSF51690. 1 hit.

Sequencei

Sequence statusi: Complete.

Q80Z29-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MNAAAEAEFN ILLATDSYKV THYKQYPPNT SKVYSYFECR EKKTENSKVR
60 70 80 90 100
KVKYEETVFY GLQYILNKYL KGKVVTKEKI QEAKEVYREH FQDDVFNERG
110 120 130 140 150
WNYILEKYDG HLPIEVKAVP EGSVIPRGNV LFTVENTDPE CYWLTNWIET
160 170 180 190 200
ILVQSWYPIT VATNSREQKK ILAKYLLETS GNLDGLEYKL HDFGYRGVSS
210 220 230 240 250
QETAGIGASA HLVNFKGTDT VAGIALIKKY YGTKDPVPGY SVPAAEHSTI
260 270 280 290 300
TAWGKDHEKD AFEHIVTQFS SVPVSVVSDS YDIYNACEKI WGEDLRHLIV
310 320 330 340 350
SRSTEAPLII RPDSGNPLDT VLKVLDILGK KFPVSENSKG YKLLPPYLRV
360 370 380 390 400
IQGDGVDINT LQEIVEGMKQ KKWSIENVSF GSGGALLQKL TRDLLNCSFK
410 420 430 440 450
CSYVVTNGLG VNVFKDPVAD PNKRSKKGRL SLHRTPAGTF VTLEEGKGDL
460 470 480 490
EEYGHDLLHT VFKNGKVTKS YSFDEVRKNA QLNMEQDVAP H
Length:491
Mass (Da):55,438
Last modified:June 1, 2003 - v1
Checksum:i78F526F0D3F579FC
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB081730 mRNA. Translation: BAC66022.1.
AY831728 mRNA. Translation: AAX49410.1.
BC085681 mRNA. Translation: AAH85681.1.
RefSeqiNP_808789.1. NM_177928.3.
UniGeneiRn.203508.

Genome annotation databases

EnsembliENSRNOT00000013043; ENSRNOP00000013043; ENSRNOG00000009754.
GeneIDi297508.
KEGGirno:297508.
UCSCiRGD:631365. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB081730 mRNA. Translation: BAC66022.1 .
AY831728 mRNA. Translation: AAX49410.1 .
BC085681 mRNA. Translation: AAH85681.1 .
RefSeqi NP_808789.1. NM_177928.3.
UniGenei Rn.203508.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2G95 X-ray 1.90 A/B 1-491 [» ]
2G96 X-ray 2.90 A/B 1-491 [» ]
2G97 X-ray 2.90 A/B 1-491 [» ]
3G8E X-ray 3.00 A/B 1-491 [» ]
ProteinModelPortali Q80Z29.
SMRi Q80Z29. Positions 9-483.
ModBasei Search...
MobiDBi Search...

PTM databases

PhosphoSitei Q80Z29.

Proteomic databases

PaxDbi Q80Z29.
PRIDEi Q80Z29.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000013043 ; ENSRNOP00000013043 ; ENSRNOG00000009754 .
GeneIDi 297508.
KEGGi rno:297508.
UCSCi RGD:631365. rat.

Organism-specific databases

CTDi 10135.
RGDi 631365. Nampt.

Phylogenomic databases

eggNOGi COG1488.
GeneTreei ENSGT00390000006647.
HOGENOMi HOG000216546.
HOVERGENi HBG000336.
InParanoidi Q80Z29.
KOi K03462.
OMAi KKFPITE.
OrthoDBi EOG7PGDQH.

Enzyme and pathway databases

UniPathwayi UPA00253 ; UER00890 .
BRENDAi 2.4.2.12. 5301.
Reactomei REACT_196405. BMAL1:CLOCK,NPAS2 activates circadian gene expression.
SABIO-RK Q80Z29.

Miscellaneous databases

EvolutionaryTracei Q80Z29.
NextBioi 642340.
PROi Q80Z29.

Gene expression databases

Genevestigatori Q80Z29.

Family and domain databases

InterProi IPR007229. Nic_PRibTrfase-Fam.
IPR016471. Nicotinamide_PRibTrfase.
IPR002638. Quinolinate_PRibosylTrfase_C.
[Graphical view ]
PANTHERi PTHR11098. PTHR11098. 1 hit.
PTHR11098:SF2. PTHR11098:SF2. 1 hit.
Pfami PF04095. NAPRTase. 1 hit.
[Graphical view ]
PIRSFi PIRSF005943. NMPRT. 1 hit.
SUPFAMi SSF51690. SSF51690. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Growth phase-dependent changes in the subcellular localization of pre-B-cell colony-enhancing factor."
    Kitani T., Okuno S., Fujisawa H.
    FEBS Lett. 544:74-78(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
  2. "NAD biosynthesis pathway mediated by nicotinamide phosphoribosyltransferase attenuates ischemic neuronal cell death."
    Xing J., Gao Y., Cao G., Chen J.
    Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
    Tissue: Brain.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Heart.
  4. "Crystal structure of visfatin/pre-B cell colony-enhancing factor 1/nicotinamide phosphoribosyltransferase, free and in complex with the anti-cancer agent FK-866."
    Kim M.-K., Lee J.H., Kim H., Park S.J., Kim S.H., Kang G.B., Lee Y.S., Kim J.B., Kim K.K., Suh S.W., Eom S.H.
    J. Mol. Biol. 362:66-77(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS) IN COMPLEXES WITH NICOTINAMIDE MONONUCLEOTIDE AND FK866, SUBUNIT.

Entry informationi

Entry nameiNAMPT_RAT
AccessioniPrimary (citable) accession number: Q80Z29
Secondary accession number(s): Q2VT47
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 5, 2005
Last sequence update: June 1, 2003
Last modified: October 29, 2014
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3