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Protein

Mucin-2

Gene

Muc2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Coats the epithelia of the intestines, airways, and other mucus membrane-containing organs. Thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces. Major constituent of both the inner and outer mucus layers of the colon and may play a role in excluding bacteria from the inner mucus layer.By similarity2 Publications

GO - Biological processi

  • apoptotic process Source: MGI
  • epithelial cell development Source: MGI
  • maintenance of gastrointestinal epithelium Source: MGI
  • negative regulation of cell migration Source: MGI
  • negative regulation of cell proliferation Source: MGI
  • positive regulation of apoptotic process Source: MGI
Complete GO annotation...

Protein family/group databases

MEROPSiI08.954.

Names & Taxonomyi

Protein namesi
Recommended name:
Mucin-21 Publication
Short name:
MUC-21 Publication
Alternative name(s):
Colonic mucin1 Publication
Short name:
MCM1 Publication
Secreted gel-forming mucinImported
Gene namesi
Name:Muc2Imported
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:1339364. Muc2.

Subcellular locationi

GO - Cellular componenti

  • inner mucus layer Source: UniProtKB
  • outer mucus layer Source: UniProtKB
  • proteinaceous extracellular matrix Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Disruption phenotypei

Aberrant intestinal crypt morphology and altered cell maturation and migration. Frequent development of adenomas in the small intestine which progress to invasive adenocarcinomas, as well as rectal tumors. Absence of inner and outer mucus layers in the colon so that bacteria are in direct contact with the colon epithelium and enter into the cells and crypts in contrast to wild-type animals which are devoid of bacteria in the inner mucus layer.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 20Sequence analysisAdd BLAST20
ChainiPRO_000037806221 – 2680Mucin-2Sequence analysisAdd BLAST2660

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi? ↔ 2659Sequence analysis
Modified residuei21PhosphoserineBy similarity1
Disulfide bondi56 ↔ 64Sequence analysis
Glycosylationi667N-linked (GlcNAc...)Sequence analysis1
Glycosylationi1227N-linked (GlcNAc...)Sequence analysis1
Glycosylationi1849N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi2575 ↔ 2622Sequence analysis
Disulfide bondi2589 ↔ 2636Sequence analysis
Disulfide bondi2598 ↔ 2652Sequence analysis
Disulfide bondi2602 ↔ 2654Sequence analysis

Post-translational modificationi

O-glycosylated.1 Publication
May undergo proteolytic cleavage in the outer mucus layer of the colon, contributing to the expanded volume and loose nature of this layer which allows for bacterial colonization in contrast to the inner mucus layer which is dense and devoid of bacteria.1 Publication
May undergo autocatalytic cleavage in vivo triggered by the low pH of the late secretory pathway.By similarity

Keywords - PTMi

Autocatalytic cleavage, Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiQ80Z19.
PeptideAtlasiQ80Z19.
PRIDEiQ80Z19.

PTM databases

SwissPalmiQ80Z19.

Expressioni

Tissue specificityi

Highly expressed in goblet cells of the colon with lower levels in the small intestine and no expression in the stomach (at protein level).2 Publications

Gene expression databases

BgeeiENSMUSG00000025515.

Interactioni

Subunit structurei

Homotrimer; disulfide-linked. Dimerizes in the endoplasmic reticulum via its C-terminal region and polymerizes via its N-terminal region by disulfide-linked trimerization (By similarity). Interacts with FCGBP. Interacts with AGR2; disulfide-linked (By similarity).By similarity

Structurei

3D structure databases

ProteinModelPortaliQ80Z19.
SMRiQ80Z19.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini33 – 238VWFD 1PROSITE-ProRule annotationAdd BLAST206
Domaini295 – 351TILSequence analysisAdd BLAST57
Domaini388 – 601VWFD 2PROSITE-ProRule annotationAdd BLAST214
Domaini857 – 1062VWFD 3PROSITE-ProRule annotationAdd BLAST206
Domaini1985 – 2197VWFD 4PROSITE-ProRule annotationAdd BLAST213
Domaini2315 – 2386VWFC 1PROSITE-ProRule annotationAdd BLAST72
Domaini2424 – 2491VWFC 2PROSITE-ProRule annotationAdd BLAST68
Domaini2575 – 2660CTCKPROSITE-ProRule annotationAdd BLAST86

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi1257 – 1913Thr-richSequence analysisAdd BLAST657

Sequence similaritiesi

Contains 1 CTCK (C-terminal cystine knot-like) domain.PROSITE-ProRule annotation
Contains 1 TIL (trypsin inhibitory-like) domain.Sequence analysis
Contains 2 VWFC domains.PROSITE-ProRule annotation
Contains 4 VWFD domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

HOGENOMiHOG000203111.
InParanoidiQ80Z19.
TreeFamiTF337106.

Family and domain databases

InterProiIPR006207. Cys_knot_C.
IPR028580. MUC2.
IPR002919. TIL_dom.
IPR014853. Unchr_dom_Cys-rich.
IPR001007. VWF_dom.
IPR001846. VWF_type-D.
IPR025155. WxxW_domain.
[Graphical view]
PANTHERiPTHR11339:SF261. PTHR11339:SF261. 2 hits.
PfamiPF08742. C8. 4 hits.
PF13330. Mucin2_WxxW. 2 hits.
PF01826. TIL. 2 hits.
PF00094. VWD. 4 hits.
[Graphical view]
SMARTiSM00832. C8. 4 hits.
SM00041. CT. 1 hit.
SM00214. VWC. 4 hits.
SM00215. VWC_out. 2 hits.
SM00216. VWD. 4 hits.
[Graphical view]
SUPFAMiSSF57567. SSF57567. 4 hits.
PROSITEiPS01185. CTCK_1. 1 hit.
PS01225. CTCK_2. 1 hit.
PS01208. VWFC_1. 2 hits.
PS50184. VWFC_2. 2 hits.
PS51233. VWFD. 4 hits.
[Graphical view]

Sequencei

Sequence statusi: Fragments.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q80Z19-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGLPLARLVA ACLVLALAKG SELQKEARSR NHVCSTWGDF HYKTFDGDVY
60 70 80 90 100
RFPGLCDYNF ASDCRDSYKE FAVHLKRGLG EAGGHSQIES ILITIKDDTI
110 120 130 140 150
YLTHKLAVVN GAMVSTPHYS SGLLIEKNDA YTKVYSRAGL SLMWNREDAL
160 170 180 190 200
MVELDSRFQN HTCGLCGDFN GMQTNYEFLS EEGIQFSAIE FGNMQKINKP
210 220 230 240 250
EVQCEDPEAV QEPESCSEHR AECERLLTSA AFEDCQTRVP VESYVRACMH
260 270 280 290 300
DRCQCPKGGA CECSTLAEFS RQCSHAGGRP ENWRTASLCP KKCPNNMVYL
310 320 330 340 350
ESSSPCVDTC SHLEVSSLCE EHYMDGCFCP EGTVYDDITG SGCIPVSQCH
360 370 380 390 400
CKLHGHLYMP GQEFTNDCEQ CVCNAGRWVC KDLPCPETCA LEGGSHITTF
410 420 430 440 450
DGKKFTFHGD CYYVLTKSEH NDSYALLGEL ASCGSTDKQT CLKTVVLLTD
460 470 480 490 500
DKKNVVAFKS GGSVLLNEME VTLPHVAASF SIFQPSSYHI VVNTKFGLRL
510 520 530 540 550
QIQLLPVMQL FVTLDQAAQG QVQGLCGNFN GLESDDFMTS GGMVEATGAG
560 570 580 590 600
FANTWKAQSS CHDKLDWLDD PCSLNIETNY AEHWCSLLKR SETPFARCHL
610 620 630 640 650
AVDPTEYYKR CKYDTCNCQN NEDCMCAALS SYARACAAKG VMLWGWRERV
660 670 680 690 700
CNKDVHACPS SQIFMYNLTT CQQTCRSLSE GDSHCLKGFA PVEGCGCPDH
710 720 730 740 750
TFMDEKGRCV PLAKCSCYHH GLYLEAGDVI LRQEERCICR NGRLQCTQVK
760 770 780 790 800
LIGHTCQYPK ILVDCNNLTA LAVRKPRPTS CQTLVAGYYH TECISGCVCP
810 820 830 840 850
DGLLDDGRGG CVEEDKCPCI HNKDLYSSGE SIKLDCNNTC TCQKGRWECT
860 870 880 890 900
RYACHSTCSI YGSGHYITFD GKHYDFDGHC SYVAVQDYCG QNSTGSFSII
910 920 930 940 950
TENVPCGTTG VTCSKAIKIF IGGTELKLVD KHRVVKQLEE GHHVPYITRE
960 970 980 990 1000
VGQYLVVEAS SGIIVIWDKK TTIFIKLDPS YKGTVCGLCG NFDDQTKNDF
1010 1020 1030 1040 1050
TTRDHMVVTS ELDFGNSWKE ASTCPDVSHN PDPCSLNPHR RSWAEKQCSI
1060 1070 1080 1090 1100
IKSRVFKVCH SKVDPTVFYE ACVHDSCSCD TGGDCDCFCS AVASYAQECT
1110 1120 1130 1140 1150
KAEACVFWRT PDLCPIFCDY YNPPDECEWH YEPCGNRSFE TCRTLNGIHS
1160 1170 1180 1190 1200
NISVSYLEGC YPRCPEDRPI YDEDLKKCVT GDKCGCYIED TRYPPGGSVP
1210 1220 1230 1240 1250
TDEICKSCTC TNTSKIECHP DEGKILNMTQ DGIFCYWEFC GPNGTVGQHF
1260 1270 1280 1290 1300
NICGSSTAIP STTTSFTTIS TPISTTPIST TITTTTVTMT TEQVPCCFWS
1310 1320 1330 1340 1350
DWINKYHPTK ENGGDRETFT HVCSAPEDIE CRAATDPKLS WEELGQKVQC
1360 1370 1380 1390 1400
NVSTGLICNN EDQYGIGEFE LCYDYEIRVN CCYPMEYCTP STISPTTSTT
1410 1420 1430 1440 1450
TLSTTPPTSS PTTLPTSSPV TSSATLPTTS SITSTISPTT SPSTATQTIS
1460 1470 1480 1490 1500
VTTSQTSSSA TPPNSSPTSS ATTSPTTSSG TSTATSPSTS PTTSSTFTTP
1510 1520 1530 1540 1550
PSTTCIDDCK WTGWLDSGKP TYDIKSGDFE LIKGVCEPHW EVQNISCRAV
1560 1570 1580 1590 1600
MHSNIPLDQL GQIVVCNKEV GLVCKNEDQE IGGIIPMRMC LNYEINVYCC
1610 1620 1630 1640 1650
NPICFTSTPS STTTETPTTT STTKTSILTS TTTQTPSPSP TTTVTPTPAP
1660 1670 1680 1690 1700
TTTQIPTSTS TTTQTTTPTP ITETSTPTST ISQTPSPAST TTVTPATTST
1710 1720 1730 1740 1750
TTETSTSTST TTQTTSPTPT VTETSTPRST TTQTPSPVPT TTVTSTPTPT
1760 1770 1780 1790 1800
IGETTTPKRP PSTSTPTSFT VPTETTTQTR PLSTTPTTLE TTRTSSWGTF
1810 1820 1830 1840 1850
SSTSPITSPS TVWTHTETQV TCCVLNEMFY GPGELVYNST HGGTCFYVNC
1860 1870 1880 1890 1900
SLDCHLQFFN WSCPSTPSTP TPSTPTPTPS QTTTPSTTSS KSTPSTPQST
1910 1920 1930 1940 1950
SPKSTLSTPT KTTPYGCPDF DPPRQVNETW WLCNCTMAIC NHDNVVEIVP
1960 1970 1980 1990 2000
LKCDPPPMPT CANGLKPVRV PDADNCCWHW ECDCYCTGWG DPHFVTFDGL
2010 2020 2030 2040 2050
YYSYQGNCTY VLVEEITPTV DNFGVYIDNY HCDANDKVSC PRTLIVRHET
2060 2070 2080 2090 2100
QEVQIKTVRM MPIEVEVQVN KQLVALPYKK YGLEVYESGI NIVVNISRLE
2110 2120 2130 2140 2150
AKISYNGLSF SIRLPYKLFV NNTKGQCGTC TNNTADDCIL PSGKIISDCE
2160 2170 2180 2190 2200
IAADEWLVND PSKPHCPHKG LTTKRPATTT PGLSLNNCTV SPVCHLIMDS
2210 2220 2230 2240 2250
LFSQCHAFVP PKHYYEACLF DSCYVPGSNM ECASVQAYAT LCAKEGVCID
2260 2270 2280 2290 2300
WRNHTQGVCS VKCPPHKQYQ ACGPEEEPTC QPSSSQNSTL LVEGCFCPEG
2310 2320 2330 2340 2350
TTKFAPGYDV CVKTCGCVGP DNVPREFGEH FEFDCKDCVC REGGSGIVCQ
2360 2370 2380 2390 2400
PKKCSGGNQT TCEEDGTYLV VETNPDDKCC NITSCKCDTK RCKAERPTCL
2410 2420 2430 2440 2450
LGFEVKTEIV PGKCCPVYSC VPKGVCVHQN AEYQPGSPVY SNKCQDCVCT
2460 2470 2480 2490 2500
NILDNSTQLN VISCTHVPCN ISCSSGFELV DVPGECCKKC QQTHCIIEGP
2510 2520 2530 2540 2550
KQQYIILKPG EIHKNPSNKC TFFSCMKINN QLISSVSNIT CPDFNPSDCV
2560 2570 2580 2590 2600
SGSITYMPNG CCKTCIPQNQ TRVPCSAVSV MKEISYNGCT KNISMNYCFG
2610 2620 2630 2640 2650
SCGTFAMYSA QVQGLDHRCS CCKEEKTSVR SVTLECPDGS ELSHTYTHIE
2660 2670 2680
SCLCQDTVCG LPQAQQVRTR RSSPRFLGRK
Length:2,680
Mass (Da):293,436
Last modified:June 16, 2009 - v2
Checksum:iD67D8664AAB15933
GO

Sequence cautioni

The sequence AAH30862 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti301E → G in AAD01593 (PubMed:9886986).Curated1
Non-adjacent residuesi1442 – 1443Curated2
Non-adjacent residuesi1758 – 1759Curated2
Sequence conflicti2120V → G in AAH30862 (PubMed:15489334).Curated1
Sequence conflicti2120V → G in AAH36168 (PubMed:15489334).Curated1
Sequence conflicti2398T → P in AAH36168 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ511872 Genomic DNA. Translation: CAD54414.1.
AJ511873, AJ511874 Genomic DNA. Translation: CAD54416.1.
AF016695 mRNA. Translation: AAD01593.1.
BC024540 mRNA. No translation available.
BC030862 mRNA. Translation: AAH30862.1. Different initiation.
BC036168 mRNA. Translation: AAH36168.1.
AK008250 mRNA. Translation: BAB25557.1.
UniGeneiMm.2041.
Mm.461296.

Genome annotation databases

UCSCiuc029wpp.2. mouse.

Cross-referencesi

Web resourcesi

Mucin database

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ511872 Genomic DNA. Translation: CAD54414.1.
AJ511873, AJ511874 Genomic DNA. Translation: CAD54416.1.
AF016695 mRNA. Translation: AAD01593.1.
BC024540 mRNA. No translation available.
BC030862 mRNA. Translation: AAH30862.1. Different initiation.
BC036168 mRNA. Translation: AAH36168.1.
AK008250 mRNA. Translation: BAB25557.1.
UniGeneiMm.2041.
Mm.461296.

3D structure databases

ProteinModelPortaliQ80Z19.
SMRiQ80Z19.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

MEROPSiI08.954.

PTM databases

SwissPalmiQ80Z19.

Proteomic databases

MaxQBiQ80Z19.
PeptideAtlasiQ80Z19.
PRIDEiQ80Z19.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

UCSCiuc029wpp.2. mouse.

Organism-specific databases

MGIiMGI:1339364. Muc2.

Phylogenomic databases

HOGENOMiHOG000203111.
InParanoidiQ80Z19.
TreeFamiTF337106.

Miscellaneous databases

ChiTaRSiMuc2. mouse.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000025515.

Family and domain databases

InterProiIPR006207. Cys_knot_C.
IPR028580. MUC2.
IPR002919. TIL_dom.
IPR014853. Unchr_dom_Cys-rich.
IPR001007. VWF_dom.
IPR001846. VWF_type-D.
IPR025155. WxxW_domain.
[Graphical view]
PANTHERiPTHR11339:SF261. PTHR11339:SF261. 2 hits.
PfamiPF08742. C8. 4 hits.
PF13330. Mucin2_WxxW. 2 hits.
PF01826. TIL. 2 hits.
PF00094. VWD. 4 hits.
[Graphical view]
SMARTiSM00832. C8. 4 hits.
SM00041. CT. 1 hit.
SM00214. VWC. 4 hits.
SM00215. VWC_out. 2 hits.
SM00216. VWD. 4 hits.
[Graphical view]
SUPFAMiSSF57567. SSF57567. 4 hits.
PROSITEiPS01185. CTCK_1. 1 hit.
PS01225. CTCK_2. 1 hit.
PS01208. VWFC_1. 2 hits.
PS50184. VWFC_2. 2 hits.
PS51233. VWFD. 4 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMUC2_MOUSE
AccessioniPrimary (citable) accession number: Q80Z19
Secondary accession number(s): Q0P637
, Q80Z17, Q8K0Q1, Q9CVG8, Q9Z2U5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 16, 2009
Last sequence update: June 16, 2009
Last modified: November 2, 2016
This is version 98 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.