Q80Z19 (MUC2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Mucin-2
Short name=MUC-2

Alternative name(s):
Colonic mucin
Short name=MCM
Secreted gel-forming mucin

Gene names

Name:

Muc2

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	2680 AA.
Sequence status	Fragments.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Coats the epithelia of the intestines, airways, and other mucus membrane-containing organs. Thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces. Major constituent of both the inner and outer mucus layers of the colon and may play a role in excluding bacteria from the inner mucus layer. Ref.6 Ref.7 UniProtKB Q02817
Subunit structure	Homotrimer; disulfide-linked. Dimerizes in the endoplasmic reticulum via its C-terminal region and polymerizes via its N-terminal region by disulfide-linked trimerization By similarity. Interacts with FCGBP. Interacts with AGR2; disulfide-linked By similarity. Ref.7 UniProtKB Q02817
Subcellular location	Secreted. Note: In the intestine, secreted into the inner and outer mucus layers. Ref.6 Ref.7
Tissue specificity	Highly expressed in goblet cells of the colon with lower levels in the small intestine and no expression in the stomach (at protein level). Ref.1 Ref.2
Post-translational modification	O-glycosylated. Ref.2 May undergo proteolytic cleavage in the outer mucus layer of the colon, contributing to the expanded volume and loose nature of this layer which allows for bacterial colonization in contrast to the inner mucus layer which is dense and devoid of bacteria. Ref.6 May undergo autocatalytic cleavage in vivo triggered by the low pH of the late secretory pathway By similarity. UniProtKB Q02817
Disruption phenotype	Aberrant intestinal crypt morphology and altered cell maturation and migration. Frequent development of adenomas in the small intestine which progress to invasive adenocarcinomas, as well as rectal tumors. Absence of inner and outer mucus layers in the colon so that bacteria are in direct contact with the colon epithelium and enter into the cells and crypts in contrast to wild-type animals which are devoid of bacteria in the inner mucus layer. Ref.5 Ref.6
Sequence similarities	Contains 1 CTCK (C-terminal cystine knot-like) domain. Contains 1 TIL (trypsin inhibitory-like) domain. Contains 2 VWFC domains. Contains 4 VWFD domains.
Sequence caution	The sequence AAH30862.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Cellular component	Secreted
Domain	Repeat Signal
PTM	Autocatalytic cleavage Disulfide bond Glycoprotein Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	apoptotic process Inferred from mutant phenotype Ref.5. Source: MGI induction of apoptosis Inferred from mutant phenotype Ref.5. Source: MGI negative regulation of cell migration Inferred from mutant phenotype Ref.5. Source: MGI negative regulation of cell proliferation Inferred from mutant phenotype Ref.5. Source: MGI
Cellular_component	inner mucus layer Inferred from direct assay Ref.6Ref.7. Source: UniProtKB outer mucus layer Inferred from direct assay Ref.6Ref.7. Source: UniProtKB proteinaceous extracellular matrix Inferred from direct assay PubMed 11352827PubMed 19411636. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 20	20	Potential
	Chain	21 – 2680	2660	Mucin-2		PRO_0000378062
Regions
	Domain	33 – 238	206	VWFD 1
	Domain	295 – 351	57	TIL
	Domain	388 – 601	214	VWFD 2
	Domain	857 – 1062	206	VWFD 3
	Domain	1985 – 2197	213	VWFD 4
	Domain	2315 – 2386	72	VWFC 1
	Domain	2424 – 2491	68	VWFC 2
	Domain	2575 – 2660	86	CTCK
	Compositional bias	1257 – 1913	657	Thr-rich
Amino acid modifications
	Modified residue	21	1	Phosphoserine By similarity UniProtKB Q02817
	Glycosylation	667	1	N-linked (GlcNAc...) Potential
	Glycosylation	1227	1	N-linked (GlcNAc...) Potential
	Glycosylation	1849	1	N-linked (GlcNAc...) Potential
	Disulfide bond	56 ↔ 64		By similarity
	Disulfide bond	2575 ↔ 2622		By similarity
	Disulfide bond	2589 ↔ 2636		By similarity
	Disulfide bond	2598 ↔ 2652		By similarity
	Disulfide bond	2602 ↔ 2654		By similarity
	Disulfide bond	? ↔ 2659		By similarity
Experimental info
	Sequence conflict	301	1	E → G in AAD01593. Ref.2
	Sequence conflict	2120	1	V → G in AAH30862. Ref.3
	Sequence conflict	2120	1	V → G in AAH36168. Ref.3
	Sequence conflict	2398	1	T → P in AAH36168. Ref.3
	Non-adjacent residues	1442 – 1443	2
	Non-adjacent residues	1758 – 1759	2

Sequences

	Sequence		Length	Mass (Da)
	Q80Z19 [UniParc]. Last modified June 16, 2009. Version 2. Checksum: D67D8664AAB15933	FASTA	2,680	293,436
	10 20 30 40 50 60 MGLPLARLVA ACLVLALAKG SELQKEARSR NHVCSTWGDF HYKTFDGDVY RFPGLCDYNF 70 80 90 100 110 120 ASDCRDSYKE FAVHLKRGLG EAGGHSQIES ILITIKDDTI YLTHKLAVVN GAMVSTPHYS 130 140 150 160 170 180 SGLLIEKNDA YTKVYSRAGL SLMWNREDAL MVELDSRFQN HTCGLCGDFN GMQTNYEFLS 190 200 210 220 230 240 EEGIQFSAIE FGNMQKINKP EVQCEDPEAV QEPESCSEHR AECERLLTSA AFEDCQTRVP 250 260 270 280 290 300 VESYVRACMH DRCQCPKGGA CECSTLAEFS RQCSHAGGRP ENWRTASLCP KKCPNNMVYL 310 320 330 340 350 360 ESSSPCVDTC SHLEVSSLCE EHYMDGCFCP EGTVYDDITG SGCIPVSQCH CKLHGHLYMP 370 380 390 400 410 420 GQEFTNDCEQ CVCNAGRWVC KDLPCPETCA LEGGSHITTF DGKKFTFHGD CYYVLTKSEH 430 440 450 460 470 480 NDSYALLGEL ASCGSTDKQT CLKTVVLLTD DKKNVVAFKS GGSVLLNEME VTLPHVAASF 490 500 510 520 530 540 SIFQPSSYHI VVNTKFGLRL QIQLLPVMQL FVTLDQAAQG QVQGLCGNFN GLESDDFMTS 550 560 570 580 590 600 GGMVEATGAG FANTWKAQSS CHDKLDWLDD PCSLNIETNY AEHWCSLLKR SETPFARCHL 610 620 630 640 650 660 AVDPTEYYKR CKYDTCNCQN NEDCMCAALS SYARACAAKG VMLWGWRERV CNKDVHACPS 670 680 690 700 710 720 SQIFMYNLTT CQQTCRSLSE GDSHCLKGFA PVEGCGCPDH TFMDEKGRCV PLAKCSCYHH 730 740 750 760 770 780 GLYLEAGDVI LRQEERCICR NGRLQCTQVK LIGHTCQYPK ILVDCNNLTA LAVRKPRPTS 790 800 810 820 830 840 CQTLVAGYYH TECISGCVCP DGLLDDGRGG CVEEDKCPCI HNKDLYSSGE SIKLDCNNTC 850 860 870 880 890 900 TCQKGRWECT RYACHSTCSI YGSGHYITFD GKHYDFDGHC SYVAVQDYCG QNSTGSFSII 910 920 930 940 950 960 TENVPCGTTG VTCSKAIKIF IGGTELKLVD KHRVVKQLEE GHHVPYITRE VGQYLVVEAS 970 980 990 1000 1010 1020 SGIIVIWDKK TTIFIKLDPS YKGTVCGLCG NFDDQTKNDF TTRDHMVVTS ELDFGNSWKE 1030 1040 1050 1060 1070 1080 ASTCPDVSHN PDPCSLNPHR RSWAEKQCSI IKSRVFKVCH SKVDPTVFYE ACVHDSCSCD 1090 1100 1110 1120 1130 1140 TGGDCDCFCS AVASYAQECT KAEACVFWRT PDLCPIFCDY YNPPDECEWH YEPCGNRSFE 1150 1160 1170 1180 1190 1200 TCRTLNGIHS NISVSYLEGC YPRCPEDRPI YDEDLKKCVT GDKCGCYIED TRYPPGGSVP 1210 1220 1230 1240 1250 1260 TDEICKSCTC TNTSKIECHP DEGKILNMTQ DGIFCYWEFC GPNGTVGQHF NICGSSTAIP 1270 1280 1290 1300 1310 1320 STTTSFTTIS TPISTTPIST TITTTTVTMT TEQVPCCFWS DWINKYHPTK ENGGDRETFT 1330 1340 1350 1360 1370 1380 HVCSAPEDIE CRAATDPKLS WEELGQKVQC NVSTGLICNN EDQYGIGEFE LCYDYEIRVN 1390 1400 1410 1420 1430 1440 CCYPMEYCTP STISPTTSTT TLSTTPPTSS PTTLPTSSPV TSSATLPTTS SITSTISPTT 1450 1460 1470 1480 1490 1500 SPSTATQTIS VTTSQTSSSA TPPNSSPTSS ATTSPTTSSG TSTATSPSTS PTTSSTFTTP 1510 1520 1530 1540 1550 1560 PSTTCIDDCK WTGWLDSGKP TYDIKSGDFE LIKGVCEPHW EVQNISCRAV MHSNIPLDQL 1570 1580 1590 1600 1610 1620 GQIVVCNKEV GLVCKNEDQE IGGIIPMRMC LNYEINVYCC NPICFTSTPS STTTETPTTT 1630 1640 1650 1660 1670 1680 STTKTSILTS TTTQTPSPSP TTTVTPTPAP TTTQIPTSTS TTTQTTTPTP ITETSTPTST 1690 1700 1710 1720 1730 1740 ISQTPSPAST TTVTPATTST TTETSTSTST TTQTTSPTPT VTETSTPRST TTQTPSPVPT 1750 1760 1770 1780 1790 1800 TTVTSTPTPT IGETTTPKRP PSTSTPTSFT VPTETTTQTR PLSTTPTTLE TTRTSSWGTF 1810 1820 1830 1840 1850 1860 SSTSPITSPS TVWTHTETQV TCCVLNEMFY GPGELVYNST HGGTCFYVNC SLDCHLQFFN 1870 1880 1890 1900 1910 1920 WSCPSTPSTP TPSTPTPTPS QTTTPSTTSS KSTPSTPQST SPKSTLSTPT KTTPYGCPDF 1930 1940 1950 1960 1970 1980 DPPRQVNETW WLCNCTMAIC NHDNVVEIVP LKCDPPPMPT CANGLKPVRV PDADNCCWHW 1990 2000 2010 2020 2030 2040 ECDCYCTGWG DPHFVTFDGL YYSYQGNCTY VLVEEITPTV DNFGVYIDNY HCDANDKVSC 2050 2060 2070 2080 2090 2100 PRTLIVRHET QEVQIKTVRM MPIEVEVQVN KQLVALPYKK YGLEVYESGI NIVVNISRLE 2110 2120 2130 2140 2150 2160 AKISYNGLSF SIRLPYKLFV NNTKGQCGTC TNNTADDCIL PSGKIISDCE IAADEWLVND 2170 2180 2190 2200 2210 2220 PSKPHCPHKG LTTKRPATTT PGLSLNNCTV SPVCHLIMDS LFSQCHAFVP PKHYYEACLF 2230 2240 2250 2260 2270 2280 DSCYVPGSNM ECASVQAYAT LCAKEGVCID WRNHTQGVCS VKCPPHKQYQ ACGPEEEPTC 2290 2300 2310 2320 2330 2340 QPSSSQNSTL LVEGCFCPEG TTKFAPGYDV CVKTCGCVGP DNVPREFGEH FEFDCKDCVC 2350 2360 2370 2380 2390 2400 REGGSGIVCQ PKKCSGGNQT TCEEDGTYLV VETNPDDKCC NITSCKCDTK RCKAERPTCL 2410 2420 2430 2440 2450 2460 LGFEVKTEIV PGKCCPVYSC VPKGVCVHQN AEYQPGSPVY SNKCQDCVCT NILDNSTQLN 2470 2480 2490 2500 2510 2520 VISCTHVPCN ISCSSGFELV DVPGECCKKC QQTHCIIEGP KQQYIILKPG EIHKNPSNKC 2530 2540 2550 2560 2570 2580 TFFSCMKINN QLISSVSNIT CPDFNPSDCV SGSITYMPNG CCKTCIPQNQ TRVPCSAVSV 2590 2600 2610 2620 2630 2640 MKEISYNGCT KNISMNYCFG SCGTFAMYSA QVQGLDHRCS CCKEEKTSVR SVTLECPDGS 2650 2660 2670 2680 ELSHTYTHIE SCLCQDTVCG LPQAQQVRTR RSSPRFLGRK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The mouse secreted gel-forming mucin gene cluster." Escande F., Porchet N., Bernigaud A., Petitprez D., Aubert J.-P., Buisine M.-P. Biochim. Biophys. Acta 1676:240-250(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-1442 AND 1759-2680, TISSUE SPECIFICITY. Strain: C57BL/6J.
[2]	"Gastrointestinal expression and partial cDNA cloning of murine Muc2." van Klinken B.J.-W., Einerhand A.W.C., Duits L.A., Makkink M.K., Tytgat K.M.A.J., Renes I.B., Verburg M., Bueller H.A., Dekker J. Am. J. Physiol. 276:G115-G124(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-301, TISSUE SPECIFICITY, GLYCOSYLATION. Strain: 129. Tissue: Colon.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1443-1758 AND 1795-2680. Strain: FVB/N. Tissue: Colon.
[4]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 2199-2680. Strain: C57BL/6J. Tissue: Small intestine.
[5]	"Colorectal cancer in mice genetically deficient in the mucin Muc2." Velcich A., Yang W., Heyer J., Fragale A., Nicholas C., Viani S., Kucherlapati R., Lipkin M., Yang K., Augenlicht L. Science 295:1726-1729(2002) [PubMed] [Europe PMC] [Abstract] Cited for: DISRUPTION PHENOTYPE.
[6]	"The inner of the two Muc2 mucin-dependent mucus layers in colon is devoid of bacteria." Johansson M.E.V., Phillipson M., Petersson J., Velcich A., Holm L., Hansson G.C. Proc. Natl. Acad. Sci. U.S.A. 105:15064-15069(2008) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE.
[7]	"Proteomic analyses of the two mucus layers of the colon barrier reveal that their main component, the Muc2 mucin, is strongly bound to the Fcgbp protein." Johansson M.E.V., Thomsson K.A., Hansson G.C. J. Proteome Res. 8:3549-3557(2009) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY, FUNCTION, INTERACTION WITH FCGBP, SUBCELLULAR LOCATION.
+	Additional computationally mapped references.

Web resources

Mucin database

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ511872 Genomic DNA. Translation: CAD54414.1. AJ511873, AJ511874 Genomic DNA. Translation: CAD54416.1. AF016695 mRNA. Translation: AAD01593.1. BC024540 mRNA. No translation available. BC030862 mRNA. Translation: AAH30862.1. Different initiation. BC036168 mRNA. Translation: AAH36168.1. AK008250 mRNA. Translation: BAB25557.1.
IPI	IPI00849211.
UniGene	Mm.2041. Mm.461296.
3D structure databases
ProteinModelPortal	Q80Z19.
SMR	Q80Z19. Positions 2260-2315.
ModBase	Search...
Proteomic databases
PaxDb	Q80Z19.
PRIDE	Q80Z19.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000026590; ENSMUSP00000026590; ENSMUSG00000025515.
UCSC	uc009kma.2. mouse.
Organism-specific databases
MGI	MGI:1339364. Muc2.
Phylogenomic databases
eggNOG	NOG12793.
GeneTree	ENSGT00700000104201.
HOGENOM	HOG000203111.
InParanoid	Q8K0Q1.
OrthoDB	EOG4QJRMP.
Gene expression databases
Genevestigator	Q80Z19.
Family and domain databases
InterPro	IPR006207. Cys_knot_C. IPR002919. TIL_dom. IPR014853. Unchr_dom_Cys-rich. IPR001007. VWF_C. IPR001846. VWF_type-D. IPR025155. WxxW_domain. [Graphical view]
Pfam	PF08742. C8. 4 hits. PF13330. Mucin2_WxxW. 2 hits. PF01826. TIL. 1 hit. PF00094. VWD. 4 hits. [Graphical view]
SMART	SM00832. C8. 4 hits. SM00041. CT. 1 hit. SM00214. VWC. 3 hits. SM00216. VWD. 4 hits. [Graphical view]
SUPFAM	SSF57567. Cysrich_TIL. 5 hits.
PROSITE	PS01185. CTCK_1. 1 hit. PS01225. CTCK_2. 1 hit. PS01208. VWFC_1. 2 hits. PS50184. VWFC_2. 2 hits. PS51233. VWFD. 4 hits. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	MUC2. mouse.
SOURCE	Search...

Entry information

Entry name

MUC2_MOUSE

Accession

Primary (citable) accession number: Q80Z19
Secondary accession number(s): Q0P637 , Q80Z17, Q8K0Q1, Q9CVG8, Q9Z2U5

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 16, 2009
Last sequence update:	June 16, 2009
Last modified:	April 3, 2013
This is version 69 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program