Q80YV2 (NIPA_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Nuclear-interacting partner of ALK

Alternative name(s):
Nuclear-interacting partner of anaplastic lymphoma kinase
Short name=mNIPA
Zinc finger C3HC-type protein 1

Gene names

Name:	Zc3hc1
Synonyms:	Nipa

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	501 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Essential component of an SCF-type E3 ligase complex, SCF(NIPA), a complex that controls mitotic entry by mediating ubiquitination and subsequent degradation of cyclin B1 (CCNB1). Its cell-cycle-dependent phosphorylation regulates the assembly of the SCF(NIPA) complex, restricting CCNB1 ubiquitination activity to interphase. Its inactivation results in nuclear accumulation of CCNB1 in interphase and premature mitotic entry By similarity. Overexpression may be able to protect from apoptosis induced by IL-3 withdrawal. Ref.1
Pathway	Protein modification; protein ubiquitination.
Subunit structure	Interacts with SKP1. Component of a SCF(NIPA) E3 complex with SKP1, RBX1 and CUL1 when not phosphorylated on Ser-353. Interacts with CCNB1 By similarity.
Subcellular location	Nucleus By similarity.
Domain	The F-box-like region is required for the interaction with SKP1 By similarity.
Post-translational modification	Phosphorylated. Phosphorylated on Ser residues at G2/M phase, but not during S and G0 phases. May also be weakly phosphorylated on Tyr residues. Ser-353 phosphorylation, a major site during the course of cell-cycle-dedendent phosphorylation, results in its dissociation from the SCF(NIPA) complex, thereby preventing CCNB1 degradation leading to mitotic entry By similarity.
Sequence similarities	Contains 1 C3HC-type zinc finger.
Sequence caution	The sequence AAH37445.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Cell cycle Cell division Mitosis Ubl conjugation pathway
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
Domain	Zinc-finger
Ligand	Metal-binding Zinc
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell division Inferred from electronic annotation. Source: UniProtKB-KW mitosis Inferred from electronic annotation. Source: UniProtKB-KW negative regulation of apoptotic process Inferred from direct assay Ref.1. Source: MGI protein ubiquitination Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	nuclear membrane Inferred from electronic annotation. Source: Compara nucleus Inferred from direct assay Ref.1. Source: MGI
Molecular_function	zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q80YV2-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q80YV2-2)

The sequence of this isoform differs from the canonical sequence as follows:
     480-501: SLSEKSRKVFRIFRQWESSSSS → LK

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 501	501	Nuclear-interacting partner of ALK		PRO_0000096850
Regions
	Zinc finger	102 – 156	55	C3HC-type
	Region	170 – 210	41	F-box-like
	Motif	395 – 401	7	Nuclear localization signal By similarity
Amino acid modifications
	Modified residue	24	1	Phosphoserine By similarity
	Modified residue	28	1	Phosphothreonine By similarity
	Modified residue	58	1	Phosphoserine By similarity
	Modified residue	62	1	Phosphoserine Ref.4
	Modified residue	320	1	Phosphoserine By similarity
	Modified residue	328	1	Phosphoserine By similarity
	Modified residue	332	1	Phosphothreonine By similarity
	Modified residue	334	1	Phosphoserine By similarity
	Modified residue	337	1	Phosphoserine By similarity
	Modified residue	343	1	Phosphoserine By similarity
	Modified residue	353	1	Phosphoserine Ref.5
	Modified residue	358	1	Phosphoserine By similarity
	Modified residue	369	1	Phosphoserine Ref.5
	Modified residue	383	1	Phosphothreonine By similarity
	Modified residue	394	1	Phosphoserine By similarity
Natural variations
	Alternative sequence	480 – 501	22	SLSEK…SSSSS → LK in isoform 2.		VSP_015219
Experimental info
	Sequence conflict	65	1	A → S in CAD61162. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 1FB522C93F8A8178	FASTA	501	55,196
	10 20 30 40 50 60 MAATSEGPLF AASIEKTWGS VVRSPEGTPQ KVRELIDEGI VPEEGGTEPK DTAATFQSVD 70 80 90 100 110 120 GSPQAEQSPL ESTSKEAFFH RVETFSSLKW AGKPPELSPL ICAKYGWVTV ECDMLKCSSC 130 140 150 160 170 180 QAFLCASLQP TFDFGRYKER CAELKKSLCS AHEKFCFWPD SPSPDRFGML PLGEPAVLIS 190 200 210 220 230 240 EFLDRFQSLC HLDLQLPSLR PEDLKTMCLT EDAVSALLHL LEDELDFHAD DRKTTSKLGS 250 260 270 280 290 300 DVQVQATACV LSLCGWACSS LEPTQLSLIT CYQCMRKVGL WGFQQIESSM TDLEASFGLT 310 320 330 340 350 360 SSPIPGVEGR PEHFPLVPES PRRMMTRSQD ATVSPGSEQS EKSPGPIVSR TRSWESSSPV 370 380 390 400 410 420 DRPELEAASP TTRSRPVTRS MGTGDSAGVE VPSSPLRRTK RARLCSSSSS DTSPRSFFDP 430 440 450 460 470 480 TSQHRDWCPW VNITLVKETK ENGETEVDAC TPAEPGWKAV LTILLAHKRS NQPAETDSMS 490 500 LSEKSRKVFR IFRQWESSSS S
	Isoform 2 [UniParc]. Checksum: F173139617A1A3AD	FASTA	481	52,824
	10 20 30 40 50 60 MAATSEGPLF AASIEKTWGS VVRSPEGTPQ KVRELIDEGI VPEEGGTEPK DTAATFQSVD 70 80 90 100 110 120 GSPQAEQSPL ESTSKEAFFH RVETFSSLKW AGKPPELSPL ICAKYGWVTV ECDMLKCSSC 130 140 150 160 170 180 QAFLCASLQP TFDFGRYKER CAELKKSLCS AHEKFCFWPD SPSPDRFGML PLGEPAVLIS 190 200 210 220 230 240 EFLDRFQSLC HLDLQLPSLR PEDLKTMCLT EDAVSALLHL LEDELDFHAD DRKTTSKLGS 250 260 270 280 290 300 DVQVQATACV LSLCGWACSS LEPTQLSLIT CYQCMRKVGL WGFQQIESSM TDLEASFGLT 310 320 330 340 350 360 SSPIPGVEGR PEHFPLVPES PRRMMTRSQD ATVSPGSEQS EKSPGPIVSR TRSWESSSPV 370 380 390 400 410 420 DRPELEAASP TTRSRPVTRS MGTGDSAGVE VPSSPLRRTK RARLCSSSSS DTSPRSFFDP 430 440 450 460 470 480 TSQHRDWCPW VNITLVKETK ENGETEVDAC TPAEPGWKAV LTILLAHKRS NQPAETDSML K

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification and characterization of a nuclear interacting partner of anaplastic lymphoma kinase (NIPA)." Ouyang T., Bai R.-Y., Bassermann F., von Klitzing C., Klumpen S., Miething C., Morris S.W., Peschel C., Duyster J. J. Biol. Chem. 278:30028-30036(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), POSSIBLE FUNCTION. Strain: Swiss Webster.
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Strain: C57BL/6J and NOD. Tissue: Bone marrow, Placenta and Thymus.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: C57BL/6J, FVB/N and FVB/N-3. Tissue: Embryo and Mammary tumor.
[4]	"Phosphoproteomic analysis of the developing mouse brain." Ballif B.A., Villen J., Beausoleil S.A., Schwartz D., Gygi S.P. Mol. Cell. Proteomics 3:1093-1101(2004) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-62, MASS SPECTROMETRY. Tissue: Embryonic brain.
[5]	"Large-scale phosphorylation analysis of mouse liver." Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-353 AND SER-369, MASS SPECTROMETRY. Tissue: Liver.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ537495 mRNA. Translation: CAD61162.1. AK088527 mRNA. Translation: BAC40404.1. AK151694 mRNA. Translation: BAE30618.1. AK167465 mRNA. Translation: BAE39549.1. BC024560 mRNA. Translation: AAH24560.1. BC037445 mRNA. Translation: AAH37445.1. Different initiation. BC050141 mRNA. Translation: AAH50141.1.
IPI	IPI00465879. IPI00649674.
RefSeq	NP_766323.1. NM_172735.2.
UniGene	Mm.29780. Mm.474686.
3D structure databases
ModBase	Search...
PTM databases
PhosphoSite	Q80YV2.
Proteomic databases
PaxDb	Q80YV2.
PRIDE	Q80YV2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000080812; ENSMUSP00000079627; ENSMUSG00000039130. ENSMUST00000102992; ENSMUSP00000100057; ENSMUSG00000039130.
GeneID	232679.
KEGG	mmu:232679.
UCSC	uc009bfc.1. mouse. uc009bfd.1. mouse.
Organism-specific databases
CTD	51530.
MGI	MGI:1916023. Zc3hc1.
Phylogenomic databases
eggNOG	NOG246473.
GeneTree	ENSGT00390000006086.
HOGENOM	HOG000039985.
HOVERGEN	HBG082030.
InParanoid	Q80YV2.
OMA	FWPDSPC.
Enzyme and pathway databases
UniPathway	UPA00143.
Gene expression databases
ArrayExpress	Q80YV2.
Bgee	Q80YV2.
CleanEx	MM_ZC3HC1.
Genevestigator	Q80YV2.
GermOnline	ENSMUSG00000039130. Mus musculus.
Family and domain databases
InterPro	IPR013909. NIPA/Rsm1. IPR012935. Znf_C3HC-like. [Graphical view]
Pfam	PF08600. Rsm1. 1 hit. PF07967. zf-C3HC. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	381189.
SOURCE	Search...

Entry information

Entry name

NIPA_MOUSE

Accession

Primary (citable) accession number: Q80YV2
Secondary accession number(s): Q3TJE6 , Q80Z11, Q8BTW5, Q8CI56, Q8R3U7

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 30, 2005
Last sequence update:	June 1, 2003
Last modified:	April 3, 2013
This is version 80 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program