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Q80YV2

- NIPA_MOUSE

UniProt

Q80YV2 - NIPA_MOUSE

Protein

Nuclear-interacting partner of ALK

Gene

Zc3hc1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 94 (01 Oct 2014)
      Sequence version 1 (01 Jun 2003)
      Previous versions | rss
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    Functioni

    Essential component of an SCF-type E3 ligase complex, SCF(NIPA), a complex that controls mitotic entry by mediating ubiquitination and subsequent degradation of cyclin B1 (CCNB1). Its cell-cycle-dependent phosphorylation regulates the assembly of the SCF(NIPA) complex, restricting CCNB1 ubiquitination activity to interphase. Its inactivation results in nuclear accumulation of CCNB1 in interphase and premature mitotic entry By similarity. Overexpression may be able to protect from apoptosis induced by IL-3 withdrawal.By similarity

    Pathwayi

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri102 – 15655C3HC-typeAdd
    BLAST

    GO - Molecular functioni

    1. protein binding Source: MGI
    2. zinc ion binding Source: InterPro

    GO - Biological processi

    1. mitotic nuclear division Source: UniProtKB-KW
    2. negative regulation of extrinsic apoptotic signaling pathway in absence of ligand Source: MGI
    3. protein ubiquitination Source: UniProtKB-UniPathway

    Keywords - Biological processi

    Cell cycle, Cell division, Mitosis, Ubl conjugation pathway

    Keywords - Ligandi

    Metal-binding, Zinc

    Enzyme and pathway databases

    UniPathwayiUPA00143.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Nuclear-interacting partner of ALK
    Alternative name(s):
    Nuclear-interacting partner of anaplastic lymphoma kinase
    Short name:
    mNIPA
    Zinc finger C3HC-type protein 1
    Gene namesi
    Name:Zc3hc1
    Synonyms:Nipa
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 6

    Organism-specific databases

    MGIiMGI:1916023. Zc3hc1.

    Subcellular locationi

    Nucleus By similarity

    GO - Cellular componenti

    1. nuclear membrane Source: Ensembl
    2. nucleus Source: MGI

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 501500Nuclear-interacting partner of ALKPRO_0000096850Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylalanineBy similarity
    Modified residuei24 – 241PhosphoserineBy similarity
    Modified residuei28 – 281PhosphothreonineBy similarity
    Modified residuei58 – 581PhosphoserineBy similarity
    Modified residuei62 – 621Phosphoserine1 Publication
    Modified residuei320 – 3201PhosphoserineBy similarity
    Modified residuei328 – 3281PhosphoserineBy similarity
    Modified residuei332 – 3321PhosphothreonineBy similarity
    Modified residuei334 – 3341PhosphoserineBy similarity
    Modified residuei337 – 3371PhosphoserineBy similarity
    Modified residuei343 – 3431PhosphoserineBy similarity
    Modified residuei353 – 3531PhosphoserineBy similarity
    Modified residuei358 – 3581PhosphoserineBy similarity
    Modified residuei369 – 3691PhosphoserineBy similarity
    Modified residuei383 – 3831PhosphothreonineBy similarity
    Modified residuei394 – 3941PhosphoserineBy similarity

    Post-translational modificationi

    Phosphorylated. Phosphorylated on Ser residues at G2/M phase, but not during S and G0 phases. May also be weakly phosphorylated on Tyr residues. Ser-353 phosphorylation, a major site during the course of cell-cycle-dedendent phosphorylation, results in its dissociation from the SCF(NIPA) complex, thereby preventing CCNB1 degradation leading to mitotic entry By similarity.By similarity

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    MaxQBiQ80YV2.
    PaxDbiQ80YV2.
    PRIDEiQ80YV2.

    PTM databases

    PhosphoSiteiQ80YV2.

    Expressioni

    Gene expression databases

    ArrayExpressiQ80YV2.
    BgeeiQ80YV2.
    CleanExiMM_ZC3HC1.
    GenevestigatoriQ80YV2.

    Interactioni

    Subunit structurei

    Interacts with SKP1. Component of a SCF(NIPA) E3 complex with SKP1, RBX1 and CUL1 when not phosphorylated on Ser-353. Interacts with CCNB1 By similarity.By similarity

    Protein-protein interaction databases

    IntActiQ80YV2. 1 interaction.
    MINTiMINT-4112467.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni170 – 21041F-box-likeAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi395 – 4017Nuclear localization signalBy similarity

    Domaini

    The F-box-like region is required for the interaction with SKP1.By similarity

    Sequence similaritiesi

    Contains 1 C3HC-type zinc finger.Curated

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri102 – 15655C3HC-typeAdd
    BLAST

    Keywords - Domaini

    Zinc-finger

    Phylogenomic databases

    eggNOGiNOG246473.
    GeneTreeiENSGT00390000006086.
    HOGENOMiHOG000039985.
    HOVERGENiHBG082030.
    InParanoidiQ80YV2.
    OMAiKWAGKPS.
    OrthoDBiEOG7TXKGG.
    PhylomeDBiQ80YV2.
    TreeFamiTF314674.

    Family and domain databases

    InterProiIPR013909. NIPA/Rsm1.
    IPR012935. Znf_C3HC-like.
    [Graphical view]
    PfamiPF08600. Rsm1. 1 hit.
    PF07967. zf-C3HC. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q80YV2-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MAATSEGPLF AASIEKTWGS VVRSPEGTPQ KVRELIDEGI VPEEGGTEPK    50
    DTAATFQSVD GSPQAEQSPL ESTSKEAFFH RVETFSSLKW AGKPPELSPL 100
    ICAKYGWVTV ECDMLKCSSC QAFLCASLQP TFDFGRYKER CAELKKSLCS 150
    AHEKFCFWPD SPSPDRFGML PLGEPAVLIS EFLDRFQSLC HLDLQLPSLR 200
    PEDLKTMCLT EDAVSALLHL LEDELDFHAD DRKTTSKLGS DVQVQATACV 250
    LSLCGWACSS LEPTQLSLIT CYQCMRKVGL WGFQQIESSM TDLEASFGLT 300
    SSPIPGVEGR PEHFPLVPES PRRMMTRSQD ATVSPGSEQS EKSPGPIVSR 350
    TRSWESSSPV DRPELEAASP TTRSRPVTRS MGTGDSAGVE VPSSPLRRTK 400
    RARLCSSSSS DTSPRSFFDP TSQHRDWCPW VNITLVKETK ENGETEVDAC 450
    TPAEPGWKAV LTILLAHKRS NQPAETDSMS LSEKSRKVFR IFRQWESSSS 500
    S 501
    Length:501
    Mass (Da):55,196
    Last modified:June 1, 2003 - v1
    Checksum:i1FB522C93F8A8178
    GO
    Isoform 2 (identifier: Q80YV2-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         480-501: SLSEKSRKVFRIFRQWESSSSS → LK

    Note: No experimental confirmation available.

    Show »
    Length:481
    Mass (Da):52,824
    Checksum:iF173139617A1A3AD
    GO

    Sequence cautioni

    The sequence AAH37445.1 differs from that shown. Reason: Erroneous initiation.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti65 – 651A → S in CAD61162. (PubMed:12748172)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei480 – 50122SLSEK…SSSSS → LK in isoform 2. 1 PublicationVSP_015219Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ537495 mRNA. Translation: CAD61162.1.
    AK088527 mRNA. Translation: BAC40404.1.
    AK151694 mRNA. Translation: BAE30618.1.
    AK167465 mRNA. Translation: BAE39549.1.
    BC024560 mRNA. Translation: AAH24560.1.
    BC037445 mRNA. Translation: AAH37445.1. Different initiation.
    BC050141 mRNA. Translation: AAH50141.1.
    CCDSiCCDS19970.1. [Q80YV2-2]
    RefSeqiNP_766323.1. NM_172735.2. [Q80YV2-2]
    XP_006505126.1. XM_006505063.1. [Q80YV2-1]
    XP_006505127.1. XM_006505064.1. [Q80YV2-2]
    UniGeneiMm.29780.

    Genome annotation databases

    EnsembliENSMUST00000080812; ENSMUSP00000079627; ENSMUSG00000039130. [Q80YV2-1]
    ENSMUST00000102992; ENSMUSP00000100057; ENSMUSG00000039130. [Q80YV2-2]
    GeneIDi232679.
    KEGGimmu:232679.
    UCSCiuc009bfc.1. mouse. [Q80YV2-1]
    uc009bfd.1. mouse. [Q80YV2-2]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ537495 mRNA. Translation: CAD61162.1 .
    AK088527 mRNA. Translation: BAC40404.1 .
    AK151694 mRNA. Translation: BAE30618.1 .
    AK167465 mRNA. Translation: BAE39549.1 .
    BC024560 mRNA. Translation: AAH24560.1 .
    BC037445 mRNA. Translation: AAH37445.1 . Different initiation.
    BC050141 mRNA. Translation: AAH50141.1 .
    CCDSi CCDS19970.1. [Q80YV2-2 ]
    RefSeqi NP_766323.1. NM_172735.2. [Q80YV2-2 ]
    XP_006505126.1. XM_006505063.1. [Q80YV2-1 ]
    XP_006505127.1. XM_006505064.1. [Q80YV2-2 ]
    UniGenei Mm.29780.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q80YV2. 1 interaction.
    MINTi MINT-4112467.

    PTM databases

    PhosphoSitei Q80YV2.

    Proteomic databases

    MaxQBi Q80YV2.
    PaxDbi Q80YV2.
    PRIDEi Q80YV2.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000080812 ; ENSMUSP00000079627 ; ENSMUSG00000039130 . [Q80YV2-1 ]
    ENSMUST00000102992 ; ENSMUSP00000100057 ; ENSMUSG00000039130 . [Q80YV2-2 ]
    GeneIDi 232679.
    KEGGi mmu:232679.
    UCSCi uc009bfc.1. mouse. [Q80YV2-1 ]
    uc009bfd.1. mouse. [Q80YV2-2 ]

    Organism-specific databases

    CTDi 51530.
    MGIi MGI:1916023. Zc3hc1.

    Phylogenomic databases

    eggNOGi NOG246473.
    GeneTreei ENSGT00390000006086.
    HOGENOMi HOG000039985.
    HOVERGENi HBG082030.
    InParanoidi Q80YV2.
    OMAi KWAGKPS.
    OrthoDBi EOG7TXKGG.
    PhylomeDBi Q80YV2.
    TreeFami TF314674.

    Enzyme and pathway databases

    UniPathwayi UPA00143 .

    Miscellaneous databases

    NextBioi 381189.
    PROi Q80YV2.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q80YV2.
    Bgeei Q80YV2.
    CleanExi MM_ZC3HC1.
    Genevestigatori Q80YV2.

    Family and domain databases

    InterProi IPR013909. NIPA/Rsm1.
    IPR012935. Znf_C3HC-like.
    [Graphical view ]
    Pfami PF08600. Rsm1. 1 hit.
    PF07967. zf-C3HC. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Identification and characterization of a nuclear interacting partner of anaplastic lymphoma kinase (NIPA)."
      Ouyang T., Bai R.-Y., Bassermann F., von Klitzing C., Klumpen S., Miething C., Morris S.W., Peschel C., Duyster J.
      J. Biol. Chem. 278:30028-30036(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), POSSIBLE FUNCTION.
      Strain: Swiss Webster.
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
      Strain: C57BL/6J and NOD.
      Tissue: Bone marrow, Placenta and Thymus.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Strain: C57BL/6J, FVB/N and FVB/N-3.
      Tissue: Embryo and Mammary tumor.
    4. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-62, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Embryonic brain.
    5. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Liver.
    6. "Large scale localization of protein phosphorylation by use of electron capture dissociation mass spectrometry."
      Sweet S.M., Bailey C.M., Cunningham D.L., Heath J.K., Cooper H.J.
      Mol. Cell. Proteomics 8:904-912(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Embryonic fibroblast.

    Entry informationi

    Entry nameiNIPA_MOUSE
    AccessioniPrimary (citable) accession number: Q80YV2
    Secondary accession number(s): Q3TJE6
    , Q80Z11, Q8BTW5, Q8CI56, Q8R3U7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 30, 2005
    Last sequence update: June 1, 2003
    Last modified: October 1, 2014
    This is version 94 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3