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Protein

Nuclear-interacting partner of ALK

Gene

Zc3hc1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Essential component of an SCF-type E3 ligase complex, SCF(NIPA), a complex that controls mitotic entry by mediating ubiquitination and subsequent degradation of cyclin B1 (CCNB1). Its cell-cycle-dependent phosphorylation regulates the assembly of the SCF(NIPA) complex, restricting CCNB1 ubiquitination activity to interphase. Its inactivation results in nuclear accumulation of CCNB1 in interphase and premature mitotic entry (By similarity). Overexpression may be able to protect from apoptosis induced by IL-3 withdrawal.By similarity

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri102 – 156C3HC-typeAdd BLAST55

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, Mitosis, Ubl conjugation pathway

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Nuclear-interacting partner of ALK
Alternative name(s):
Nuclear-interacting partner of anaplastic lymphoma kinase
Short name:
mNIPA
Zinc finger C3HC-type protein 1
Gene namesi
Name:Zc3hc1
Synonyms:Nipa
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:1916023. Zc3hc1.

Subcellular locationi

GO - Cellular componenti

  • nuclear membrane Source: MGI
  • nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000968502 – 501Nuclear-interacting partner of ALKAdd BLAST500

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei24PhosphoserineBy similarity1
Modified residuei28PhosphothreonineBy similarity1
Modified residuei58PhosphoserineBy similarity1
Modified residuei62PhosphoserineCombined sources1
Modified residuei68PhosphoserineCombined sources1
Modified residuei84PhosphothreonineBy similarity1
Modified residuei320PhosphoserineBy similarity1
Modified residuei328PhosphoserineBy similarity1
Modified residuei332PhosphothreonineBy similarity1
Modified residuei334PhosphoserineCombined sources1
Modified residuei337PhosphoserineCombined sources1
Modified residuei343PhosphoserineCombined sources1
Modified residuei353PhosphoserineCombined sources1
Modified residuei358PhosphoserineCombined sources1
Modified residuei369PhosphoserineCombined sources1
Modified residuei380PhosphoserineBy similarity1
Modified residuei383PhosphothreonineBy similarity1
Modified residuei394PhosphoserineBy similarity1
Modified residuei406PhosphoserineCombined sources1
Modified residuei482PhosphoserineBy similarity1

Post-translational modificationi

Phosphorylated. Phosphorylated on Ser residues at G2/M phase, but not during S and G0 phases. May also be weakly phosphorylated on Tyr residues. Ser-353 phosphorylation, a major site during the course of cell-cycle-dedendent phosphorylation, results in its dissociation from the SCF(NIPA) complex, thereby preventing CCNB1 degradation leading to mitotic entry (By similarity).By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ80YV2.
PaxDbiQ80YV2.
PeptideAtlasiQ80YV2.
PRIDEiQ80YV2.

PTM databases

iPTMnetiQ80YV2.
PhosphoSitePlusiQ80YV2.

Expressioni

Gene expression databases

BgeeiENSMUSG00000039130.
CleanExiMM_ZC3HC1.
ExpressionAtlasiQ80YV2. baseline and differential.
GenevisibleiQ80YV2. MM.

Interactioni

Subunit structurei

Interacts with SKP1. Component of a SCF(NIPA) E3 complex with SKP1, RBX1 and CUL1 when not phosphorylated on Ser-353. Interacts with CCNB1 (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

IntActiQ80YV2. 2 interactors.
MINTiMINT-4112467.
STRINGi10090.ENSMUSP00000100057.

Structurei

3D structure databases

ProteinModelPortaliQ80YV2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni170 – 210F-box-likeAdd BLAST41

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi395 – 401Nuclear localization signalBy similarity7

Domaini

The F-box-like region is required for the interaction with SKP1.By similarity

Sequence similaritiesi

Contains 1 C3HC-type zinc finger.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri102 – 156C3HC-typeAdd BLAST55

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG4765. Eukaryota.
ENOG410ZQX4. LUCA.
GeneTreeiENSGT00390000006086.
HOGENOMiHOG000039985.
HOVERGENiHBG082030.
InParanoidiQ80YV2.
OMAiYSLKWAG.
OrthoDBiEOG091G0HKR.
PhylomeDBiQ80YV2.
TreeFamiTF314674.

Family and domain databases

InterProiIPR013909. NIPA/Rsm1.
IPR012935. Znf_C3HC-like.
[Graphical view]
PfamiPF08600. Rsm1. 1 hit.
PF07967. zf-C3HC. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q80YV2-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAATSEGPLF AASIEKTWGS VVRSPEGTPQ KVRELIDEGI VPEEGGTEPK
60 70 80 90 100
DTAATFQSVD GSPQAEQSPL ESTSKEAFFH RVETFSSLKW AGKPPELSPL
110 120 130 140 150
ICAKYGWVTV ECDMLKCSSC QAFLCASLQP TFDFGRYKER CAELKKSLCS
160 170 180 190 200
AHEKFCFWPD SPSPDRFGML PLGEPAVLIS EFLDRFQSLC HLDLQLPSLR
210 220 230 240 250
PEDLKTMCLT EDAVSALLHL LEDELDFHAD DRKTTSKLGS DVQVQATACV
260 270 280 290 300
LSLCGWACSS LEPTQLSLIT CYQCMRKVGL WGFQQIESSM TDLEASFGLT
310 320 330 340 350
SSPIPGVEGR PEHFPLVPES PRRMMTRSQD ATVSPGSEQS EKSPGPIVSR
360 370 380 390 400
TRSWESSSPV DRPELEAASP TTRSRPVTRS MGTGDSAGVE VPSSPLRRTK
410 420 430 440 450
RARLCSSSSS DTSPRSFFDP TSQHRDWCPW VNITLVKETK ENGETEVDAC
460 470 480 490 500
TPAEPGWKAV LTILLAHKRS NQPAETDSMS LSEKSRKVFR IFRQWESSSS

S
Length:501
Mass (Da):55,196
Last modified:June 1, 2003 - v1
Checksum:i1FB522C93F8A8178
GO
Isoform 2 (identifier: Q80YV2-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     480-501: SLSEKSRKVFRIFRQWESSSSS → LK

Note: No experimental confirmation available.
Show »
Length:481
Mass (Da):52,824
Checksum:iF173139617A1A3AD
GO

Sequence cautioni

The sequence AAH37445 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti65A → S in CAD61162 (PubMed:12748172).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_015219480 – 501SLSEK…SSSSS → LK in isoform 2. 1 PublicationAdd BLAST22

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ537495 mRNA. Translation: CAD61162.1.
AK088527 mRNA. Translation: BAC40404.1.
AK151694 mRNA. Translation: BAE30618.1.
AK167465 mRNA. Translation: BAE39549.1.
BC024560 mRNA. Translation: AAH24560.1.
BC037445 mRNA. Translation: AAH37445.1. Different initiation.
BC050141 mRNA. Translation: AAH50141.1.
CCDSiCCDS19970.1. [Q80YV2-2]
CCDS80508.1. [Q80YV2-1]
RefSeqiNP_001298015.1. NM_001311086.1. [Q80YV2-1]
NP_766323.1. NM_172735.2. [Q80YV2-2]
XP_017177060.1. XM_017321571.1. [Q80YV2-2]
UniGeneiMm.29780.

Genome annotation databases

EnsembliENSMUST00000080812; ENSMUSP00000079627; ENSMUSG00000039130. [Q80YV2-1]
ENSMUST00000102992; ENSMUSP00000100057; ENSMUSG00000039130. [Q80YV2-2]
GeneIDi232679.
KEGGimmu:232679.
UCSCiuc009bfc.1. mouse. [Q80YV2-1]
uc009bfd.1. mouse. [Q80YV2-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ537495 mRNA. Translation: CAD61162.1.
AK088527 mRNA. Translation: BAC40404.1.
AK151694 mRNA. Translation: BAE30618.1.
AK167465 mRNA. Translation: BAE39549.1.
BC024560 mRNA. Translation: AAH24560.1.
BC037445 mRNA. Translation: AAH37445.1. Different initiation.
BC050141 mRNA. Translation: AAH50141.1.
CCDSiCCDS19970.1. [Q80YV2-2]
CCDS80508.1. [Q80YV2-1]
RefSeqiNP_001298015.1. NM_001311086.1. [Q80YV2-1]
NP_766323.1. NM_172735.2. [Q80YV2-2]
XP_017177060.1. XM_017321571.1. [Q80YV2-2]
UniGeneiMm.29780.

3D structure databases

ProteinModelPortaliQ80YV2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ80YV2. 2 interactors.
MINTiMINT-4112467.
STRINGi10090.ENSMUSP00000100057.

PTM databases

iPTMnetiQ80YV2.
PhosphoSitePlusiQ80YV2.

Proteomic databases

EPDiQ80YV2.
PaxDbiQ80YV2.
PeptideAtlasiQ80YV2.
PRIDEiQ80YV2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000080812; ENSMUSP00000079627; ENSMUSG00000039130. [Q80YV2-1]
ENSMUST00000102992; ENSMUSP00000100057; ENSMUSG00000039130. [Q80YV2-2]
GeneIDi232679.
KEGGimmu:232679.
UCSCiuc009bfc.1. mouse. [Q80YV2-1]
uc009bfd.1. mouse. [Q80YV2-2]

Organism-specific databases

CTDi51530.
MGIiMGI:1916023. Zc3hc1.

Phylogenomic databases

eggNOGiKOG4765. Eukaryota.
ENOG410ZQX4. LUCA.
GeneTreeiENSGT00390000006086.
HOGENOMiHOG000039985.
HOVERGENiHBG082030.
InParanoidiQ80YV2.
OMAiYSLKWAG.
OrthoDBiEOG091G0HKR.
PhylomeDBiQ80YV2.
TreeFamiTF314674.

Enzyme and pathway databases

UniPathwayiUPA00143.

Miscellaneous databases

PROiQ80YV2.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000039130.
CleanExiMM_ZC3HC1.
ExpressionAtlasiQ80YV2. baseline and differential.
GenevisibleiQ80YV2. MM.

Family and domain databases

InterProiIPR013909. NIPA/Rsm1.
IPR012935. Znf_C3HC-like.
[Graphical view]
PfamiPF08600. Rsm1. 1 hit.
PF07967. zf-C3HC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNIPA_MOUSE
AccessioniPrimary (citable) accession number: Q80YV2
Secondary accession number(s): Q3TJE6
, Q80Z11, Q8BTW5, Q8CI56, Q8R3U7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 30, 2005
Last sequence update: June 1, 2003
Last modified: November 2, 2016
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.