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Q80YV2

- NIPA_MOUSE

UniProt

Q80YV2 - NIPA_MOUSE

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Protein

Nuclear-interacting partner of ALK

Gene

Zc3hc1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Essential component of an SCF-type E3 ligase complex, SCF(NIPA), a complex that controls mitotic entry by mediating ubiquitination and subsequent degradation of cyclin B1 (CCNB1). Its cell-cycle-dependent phosphorylation regulates the assembly of the SCF(NIPA) complex, restricting CCNB1 ubiquitination activity to interphase. Its inactivation results in nuclear accumulation of CCNB1 in interphase and premature mitotic entry (By similarity). Overexpression may be able to protect from apoptosis induced by IL-3 withdrawal.By similarity

Pathwayi

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri102 – 15655C3HC-typeAdd
BLAST

GO - Molecular functioni

  1. zinc ion binding Source: InterPro

GO - Biological processi

  1. mitotic nuclear division Source: UniProtKB-KW
  2. negative regulation of extrinsic apoptotic signaling pathway in absence of ligand Source: MGI
  3. protein ubiquitination Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, Mitosis, Ubl conjugation pathway

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Nuclear-interacting partner of ALK
Alternative name(s):
Nuclear-interacting partner of anaplastic lymphoma kinase
Short name:
mNIPA
Zinc finger C3HC-type protein 1
Gene namesi
Name:Zc3hc1
Synonyms:Nipa
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 6

Organism-specific databases

MGIiMGI:1916023. Zc3hc1.

Subcellular locationi

Nucleus By similarity

GO - Cellular componenti

  1. nuclear membrane Source: Ensembl
  2. nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 501500Nuclear-interacting partner of ALKPRO_0000096850Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity
Modified residuei24 – 241PhosphoserineBy similarity
Modified residuei28 – 281PhosphothreonineBy similarity
Modified residuei58 – 581PhosphoserineBy similarity
Modified residuei62 – 621Phosphoserine1 Publication
Modified residuei320 – 3201PhosphoserineBy similarity
Modified residuei328 – 3281PhosphoserineBy similarity
Modified residuei332 – 3321PhosphothreonineBy similarity
Modified residuei334 – 3341PhosphoserineBy similarity
Modified residuei337 – 3371PhosphoserineBy similarity
Modified residuei343 – 3431PhosphoserineBy similarity
Modified residuei353 – 3531PhosphoserineBy similarity
Modified residuei358 – 3581PhosphoserineBy similarity
Modified residuei369 – 3691PhosphoserineBy similarity
Modified residuei383 – 3831PhosphothreonineBy similarity
Modified residuei394 – 3941PhosphoserineBy similarity

Post-translational modificationi

Phosphorylated. Phosphorylated on Ser residues at G2/M phase, but not during S and G0 phases. May also be weakly phosphorylated on Tyr residues. Ser-353 phosphorylation, a major site during the course of cell-cycle-dedendent phosphorylation, results in its dissociation from the SCF(NIPA) complex, thereby preventing CCNB1 degradation leading to mitotic entry (By similarity).By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiQ80YV2.
PaxDbiQ80YV2.
PRIDEiQ80YV2.

PTM databases

PhosphoSiteiQ80YV2.

Expressioni

Gene expression databases

BgeeiQ80YV2.
CleanExiMM_ZC3HC1.
ExpressionAtlasiQ80YV2. baseline and differential.
GenevestigatoriQ80YV2.

Interactioni

Subunit structurei

Interacts with SKP1. Component of a SCF(NIPA) E3 complex with SKP1, RBX1 and CUL1 when not phosphorylated on Ser-353. Interacts with CCNB1 (By similarity).By similarity

Protein-protein interaction databases

IntActiQ80YV2. 1 interaction.
MINTiMINT-4112467.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni170 – 21041F-box-likeAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi395 – 4017Nuclear localization signalBy similarity

Domaini

The F-box-like region is required for the interaction with SKP1.By similarity

Sequence similaritiesi

Contains 1 C3HC-type zinc finger.Curated

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri102 – 15655C3HC-typeAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiNOG246473.
GeneTreeiENSGT00390000006086.
HOGENOMiHOG000039985.
HOVERGENiHBG082030.
InParanoidiQ80YV2.
OMAiKWAGKPS.
OrthoDBiEOG7TXKGG.
PhylomeDBiQ80YV2.
TreeFamiTF314674.

Family and domain databases

InterProiIPR013909. NIPA/Rsm1.
IPR012935. Znf_C3HC-like.
[Graphical view]
PfamiPF08600. Rsm1. 1 hit.
PF07967. zf-C3HC. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q80YV2-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAATSEGPLF AASIEKTWGS VVRSPEGTPQ KVRELIDEGI VPEEGGTEPK
60 70 80 90 100
DTAATFQSVD GSPQAEQSPL ESTSKEAFFH RVETFSSLKW AGKPPELSPL
110 120 130 140 150
ICAKYGWVTV ECDMLKCSSC QAFLCASLQP TFDFGRYKER CAELKKSLCS
160 170 180 190 200
AHEKFCFWPD SPSPDRFGML PLGEPAVLIS EFLDRFQSLC HLDLQLPSLR
210 220 230 240 250
PEDLKTMCLT EDAVSALLHL LEDELDFHAD DRKTTSKLGS DVQVQATACV
260 270 280 290 300
LSLCGWACSS LEPTQLSLIT CYQCMRKVGL WGFQQIESSM TDLEASFGLT
310 320 330 340 350
SSPIPGVEGR PEHFPLVPES PRRMMTRSQD ATVSPGSEQS EKSPGPIVSR
360 370 380 390 400
TRSWESSSPV DRPELEAASP TTRSRPVTRS MGTGDSAGVE VPSSPLRRTK
410 420 430 440 450
RARLCSSSSS DTSPRSFFDP TSQHRDWCPW VNITLVKETK ENGETEVDAC
460 470 480 490 500
TPAEPGWKAV LTILLAHKRS NQPAETDSMS LSEKSRKVFR IFRQWESSSS

S
Length:501
Mass (Da):55,196
Last modified:June 1, 2003 - v1
Checksum:i1FB522C93F8A8178
GO
Isoform 2 (identifier: Q80YV2-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     480-501: SLSEKSRKVFRIFRQWESSSSS → LK

Note: No experimental confirmation available.

Show »
Length:481
Mass (Da):52,824
Checksum:iF173139617A1A3AD
GO

Sequence cautioni

The sequence AAH37445.1 differs from that shown. Reason: Erroneous initiation.

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti65 – 651A → S in CAD61162. (PubMed:12748172)Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei480 – 50122SLSEK…SSSSS → LK in isoform 2. 1 PublicationVSP_015219Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ537495 mRNA. Translation: CAD61162.1.
AK088527 mRNA. Translation: BAC40404.1.
AK151694 mRNA. Translation: BAE30618.1.
AK167465 mRNA. Translation: BAE39549.1.
BC024560 mRNA. Translation: AAH24560.1.
BC037445 mRNA. Translation: AAH37445.1. Different initiation.
BC050141 mRNA. Translation: AAH50141.1.
CCDSiCCDS19970.1. [Q80YV2-2]
RefSeqiNP_766323.1. NM_172735.2. [Q80YV2-2]
XP_006505126.1. XM_006505063.1. [Q80YV2-1]
XP_006505127.1. XM_006505064.1. [Q80YV2-2]
UniGeneiMm.29780.

Genome annotation databases

EnsembliENSMUST00000080812; ENSMUSP00000079627; ENSMUSG00000039130. [Q80YV2-1]
ENSMUST00000102992; ENSMUSP00000100057; ENSMUSG00000039130. [Q80YV2-2]
GeneIDi232679.
KEGGimmu:232679.
UCSCiuc009bfc.1. mouse. [Q80YV2-1]
uc009bfd.1. mouse. [Q80YV2-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ537495 mRNA. Translation: CAD61162.1 .
AK088527 mRNA. Translation: BAC40404.1 .
AK151694 mRNA. Translation: BAE30618.1 .
AK167465 mRNA. Translation: BAE39549.1 .
BC024560 mRNA. Translation: AAH24560.1 .
BC037445 mRNA. Translation: AAH37445.1 . Different initiation.
BC050141 mRNA. Translation: AAH50141.1 .
CCDSi CCDS19970.1. [Q80YV2-2 ]
RefSeqi NP_766323.1. NM_172735.2. [Q80YV2-2 ]
XP_006505126.1. XM_006505063.1. [Q80YV2-1 ]
XP_006505127.1. XM_006505064.1. [Q80YV2-2 ]
UniGenei Mm.29780.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q80YV2. 1 interaction.
MINTi MINT-4112467.

PTM databases

PhosphoSitei Q80YV2.

Proteomic databases

MaxQBi Q80YV2.
PaxDbi Q80YV2.
PRIDEi Q80YV2.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000080812 ; ENSMUSP00000079627 ; ENSMUSG00000039130 . [Q80YV2-1 ]
ENSMUST00000102992 ; ENSMUSP00000100057 ; ENSMUSG00000039130 . [Q80YV2-2 ]
GeneIDi 232679.
KEGGi mmu:232679.
UCSCi uc009bfc.1. mouse. [Q80YV2-1 ]
uc009bfd.1. mouse. [Q80YV2-2 ]

Organism-specific databases

CTDi 51530.
MGIi MGI:1916023. Zc3hc1.

Phylogenomic databases

eggNOGi NOG246473.
GeneTreei ENSGT00390000006086.
HOGENOMi HOG000039985.
HOVERGENi HBG082030.
InParanoidi Q80YV2.
OMAi KWAGKPS.
OrthoDBi EOG7TXKGG.
PhylomeDBi Q80YV2.
TreeFami TF314674.

Enzyme and pathway databases

UniPathwayi UPA00143 .

Miscellaneous databases

NextBioi 381189.
PROi Q80YV2.
SOURCEi Search...

Gene expression databases

Bgeei Q80YV2.
CleanExi MM_ZC3HC1.
ExpressionAtlasi Q80YV2. baseline and differential.
Genevestigatori Q80YV2.

Family and domain databases

InterProi IPR013909. NIPA/Rsm1.
IPR012935. Znf_C3HC-like.
[Graphical view ]
Pfami PF08600. Rsm1. 1 hit.
PF07967. zf-C3HC. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Identification and characterization of a nuclear interacting partner of anaplastic lymphoma kinase (NIPA)."
    Ouyang T., Bai R.-Y., Bassermann F., von Klitzing C., Klumpen S., Miething C., Morris S.W., Peschel C., Duyster J.
    J. Biol. Chem. 278:30028-30036(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), POSSIBLE FUNCTION.
    Strain: Swiss Webster.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Strain: C57BL/6J and NOD.
    Tissue: Bone marrow, Placenta and Thymus.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: C57BL/6J, FVB/N and FVB/N-3.
    Tissue: Embryo and Mammary tumor.
  4. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-62, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic brain.
  5. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  6. "Large scale localization of protein phosphorylation by use of electron capture dissociation mass spectrometry."
    Sweet S.M., Bailey C.M., Cunningham D.L., Heath J.K., Cooper H.J.
    Mol. Cell. Proteomics 8:904-912(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic fibroblast.

Entry informationi

Entry nameiNIPA_MOUSE
AccessioniPrimary (citable) accession number: Q80YV2
Secondary accession number(s): Q3TJE6
, Q80Z11, Q8BTW5, Q8CI56, Q8R3U7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 30, 2005
Last sequence update: June 1, 2003
Last modified: October 29, 2014
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3