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Q80YS6

- AFAP1_MOUSE

UniProt

Q80YS6 - AFAP1_MOUSE

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Protein
Actin filament-associated protein 1
Gene
Afap1, Kiaa3018
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Can cross-link actin filaments into both network and bundle structures. May modulate changes in actin filament integrity and induce lamellipodia formation. May function as an adapter molecule that links other proteins, such as SRC and PKC to the actin cytoskeleton By similarity.

Keywords - Ligandi

Actin-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Actin filament-associated protein 1
Alternative name(s):
110 kDa actin filament-associated protein
Short name:
AFAP-110
Gene namesi
Name:Afap1
Synonyms:Kiaa3018
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 5

Organism-specific databases

MGIiMGI:1917542. Afap1.

Subcellular locationi

Cytoplasmcytoskeleton By similarity
Note: Localizes with stress fibers in quiescent cells, concentrated in cell motility structures such as lamellipodia, filopodia and membrane ruffles By similarity.

GO - Cellular componenti

  1. actin cytoskeleton Source: Ensembl
  2. cytoplasm Source: UniProtKB-KW
  3. focal adhesion Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 731731Actin filament-associated protein 1
PRO_0000317659Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionine By similarity
Modified residuei283 – 2831Phosphoserine By similarity
Modified residuei284 – 2841Phosphoserine By similarity
Modified residuei549 – 5491Phosphoserine By similarity
Modified residuei665 – 6651Phosphoserine By similarity
Modified residuei666 – 6661Phosphoserine By similarity
Modified residuei669 – 6691Phosphoserine1 Publication
Modified residuei680 – 6801Phosphoserine By similarity
Modified residuei688 – 6881Phosphoserine By similarity

Post-translational modificationi

Phosphorylated on tyrosine residues by SRC By similarity.

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ80YS6.
PRIDEiQ80YS6.

PTM databases

PhosphoSiteiQ80YS6.

Expressioni

Gene expression databases

BgeeiQ80YS6.
CleanExiMM_AFAP1.
GenevestigatoriQ80YS6.

Interactioni

Subunit structurei

Monomer and homomultimer. Interacts via its C-terminus with F-actin; probably involving AFAP1 multimers. Interacts with activated SRC SH3-SH2 domains. Interacts via its PH 1 domain with PRKCA, PRKCB and PRKCI By similarity.

Protein-protein interaction databases

IntActiQ80YS6. 1 interaction.
MINTiMINT-7300872.
STRINGi10090.ENSMUSP00000067779.

Structurei

3D structure databases

ProteinModelPortaliQ80YS6.
SMRiQ80YS6. Positions 343-438.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini152 – 24897PH 1
Add
BLAST
Domaini348 – 44295PH 2
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni595 – 63844Interaction with F-actin By similarity
Add
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili558 – 64992 Reviewed prediction
Add
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi70 – 734SH3-binding By similarity
Motifi93 – 964SH2-binding 1 By similarity
Motifi452 – 4576SH2-binding 2 By similarity

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi59 – 10547Pro-rich
Add
BLAST

Sequence similaritiesi

Contains 2 PH domains.

Keywords - Domaini

Coiled coil, Repeat, SH3-binding

Phylogenomic databases

eggNOGiNOG48103.
GeneTreeiENSGT00390000003998.
HOGENOMiHOG000033832.
HOVERGENiHBG106875.
InParanoidiQ80YS6.
OMAiAGRKTQV.
OrthoDBiEOG7KQ217.
PhylomeDBiQ80YS6.
TreeFamiTF332622.

Family and domain databases

Gene3Di2.30.29.30. 2 hits.
InterProiIPR001849. PH_domain.
IPR011993. PH_like_dom.
[Graphical view]
PfamiPF00169. PH. 2 hits.
[Graphical view]
SMARTiSM00233. PH. 2 hits.
[Graphical view]
PROSITEiPS50003. PH_DOMAIN. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q80YS6-1 [UniParc]FASTAAdd to Basket

« Hide

MEELIVELRL FLELLDHEYL TSTVREKKAV LTNILLRLQS SKGFEVKDHA    50
QKAEANNLPA PPQMPLPEIP QPWLPPDSGP PPLPTSSLPE GYYEEAVPLS 100
PGKAPEYITS NYDSDAMSSS YESYDEEEED GKGKKTRHQW PSEEASMDLV 150
KDAKICAFLL RKKRFGQWTK LLCVIKDTKL LCYKSSKDQQ PQMELPLQGC 200
SITYIPRDSK KKKHELKITQ QGTDPLVLAV QSKEQAEQWL KVIKEAYSGC 250
SGPVDPECSP PPSTSAPVNK AELEKKLSSE RPSSDGEGGV ENGVTTCNGK 300
EQAKRKKPSK SEAKGTVSKV TGKKITKIIG LGKKKPSTDE QTSSAEEDVP 350
TCGYLNVLSN SRWRERWCRV KDSKLILHKD RADLKTHLVS IPLRGCEVIP 400
GLDSKHPLTF RLLRNGQEVA VLEASSSEDM GRWIGILLAE TGSSTDPGAL 450
HYDYIDVEMS ANVIQTAKQT FCFMNRRAVS TSPYLGSLSN GYAHPSGTAL 500
HYDDVPCVNG SLKNKKPPAS SNGVPVKGKA PSSQQKKVET AGGVKRTASN 550
AEQYKYGKNR VEADAKRLQS KEEELLKRKE ALRNRLAQLR KERKDLRAAI 600
EVNAGRKTQA ALEDKLKRLE EECKQREAER VSLELELTEV KESLKKALAG 650
GVTLGLAIEP RSGTSSPQSP VFRHRTLENS PISSCDTSDA EGPLPVNSAA 700
VLKKSQPSSG SSPCRGHVLQ KAKEWELKNG T 731
Length:731
Mass (Da):80,615
Last modified:June 1, 2003 - v1
Checksum:i58D3D31BED5C2FCD
GO

Sequence cautioni

The sequence BAC98293.1 differs from that shown. Reason: Erroneous initiation.

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti43 – 431G → R in BAE28086. 1 Publication
Sequence conflicti52 – 521K → R in BAE28086. 1 Publication
Sequence conflicti232 – 2321S → G in BAC98293. 1 Publication
Sequence conflicti249 – 2491G → D in BAC98293. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK129483 mRNA. Translation: BAC98293.1. Different initiation.
AK147706 mRNA. Translation: BAE28086.1.
AK148000 mRNA. Translation: BAE28280.1.
AK161385 mRNA. Translation: BAE36364.1.
BC050814 mRNA. Translation: AAH50814.1.
CCDSiCCDS19236.1.
RefSeqiNP_081649.1. NM_027373.2.
UniGeneiMm.125167.

Genome annotation databases

EnsembliENSMUST00000064571; ENSMUSP00000067779; ENSMUSG00000029094.
GeneIDi70292.
KEGGimmu:70292.
UCSCiuc008xej.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK129483 mRNA. Translation: BAC98293.1 . Different initiation.
AK147706 mRNA. Translation: BAE28086.1 .
AK148000 mRNA. Translation: BAE28280.1 .
AK161385 mRNA. Translation: BAE36364.1 .
BC050814 mRNA. Translation: AAH50814.1 .
CCDSi CCDS19236.1.
RefSeqi NP_081649.1. NM_027373.2.
UniGenei Mm.125167.

3D structure databases

ProteinModelPortali Q80YS6.
SMRi Q80YS6. Positions 343-438.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q80YS6. 1 interaction.
MINTi MINT-7300872.
STRINGi 10090.ENSMUSP00000067779.

PTM databases

PhosphoSitei Q80YS6.

Proteomic databases

PaxDbi Q80YS6.
PRIDEi Q80YS6.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000064571 ; ENSMUSP00000067779 ; ENSMUSG00000029094 .
GeneIDi 70292.
KEGGi mmu:70292.
UCSCi uc008xej.1. mouse.

Organism-specific databases

CTDi 60312.
MGIi MGI:1917542. Afap1.
Rougei Search...

Phylogenomic databases

eggNOGi NOG48103.
GeneTreei ENSGT00390000003998.
HOGENOMi HOG000033832.
HOVERGENi HBG106875.
InParanoidi Q80YS6.
OMAi AGRKTQV.
OrthoDBi EOG7KQ217.
PhylomeDBi Q80YS6.
TreeFami TF332622.

Miscellaneous databases

ChiTaRSi AFAP1. mouse.
NextBioi 331322.
PROi Q80YS6.
SOURCEi Search...

Gene expression databases

Bgeei Q80YS6.
CleanExi MM_AFAP1.
Genevestigatori Q80YS6.

Family and domain databases

Gene3Di 2.30.29.30. 2 hits.
InterProi IPR001849. PH_domain.
IPR011993. PH_like_dom.
[Graphical view ]
Pfami PF00169. PH. 2 hits.
[Graphical view ]
SMARTi SM00233. PH. 2 hits.
[Graphical view ]
PROSITEi PS50003. PH_DOMAIN. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Prediction of the coding sequences of mouse homologues of KIAA gene: III. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
    Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S., Saga Y., Nagase T., Ohara O., Koga H.
    DNA Res. 10:167-180(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Melanocyte, Placenta and Testis.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Testis.
  4. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-669, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.

Entry informationi

Entry nameiAFAP1_MOUSE
AccessioniPrimary (citable) accession number: Q80YS6
Secondary accession number(s): Q3UGX1, Q6ZPE4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: June 1, 2003
Last modified: September 3, 2014
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3