Claspin - Mus musculus (Mouse)

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Q80YR7 (CLSPN_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 64. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Claspin

Gene names

Name:

Clspn

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	1315 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Required for checkpoint mediated cell cycle arrest in response to inhibition of DNA replication or to DNA damage induced by both ionizing and UV irradiation. Adapter protein which binds to BRCA1 and the checkpoint kinase CHEK1 and facilitates the ATR-dependent phosphorylation of both proteins. Can also bind specifically to branched DNA structures and may associate with S-phase chromatin following formation of the pre-replication complex (pre-RC). This may indicate a role for this protein as a sensor which monitors the integrity of DNA replication forks By similarity.
Subunit structure	Interacts (phosphorylation-dependent) with CHEK1; regulates CLSPN function in checkpoint for DNA damage and replication. Interacts with ATR and RAD9A and these interactions are slightly reduced during checkpoint activation. Interacts with BRCA1 and this interaction increases during checkpoint activation. Interacts with TIMELESS By similarity.
Subcellular location	Nucleus By similarity. Chromosome By similarity.
Domain	The C-terminus of the protein contains 3 potential CHEK1-binding motifs (CKB motifs). Potential phosphorylation sites within CKB motif 1 and CKB motif 2 are required for interaction with CHEK1 By similarity.
Post-translational modification	Phosphorylated. Undergoes ATR-dependent phosphorylation by CHEK1 during activation of DNA replication or damage checkpoints. Phosphorylation by CSNK1G1/CK1 promotes CHEK1 binding By similarity. Ubiquitinated by the anaphase promoting complex/cyclosome (APC/C) during G1 phase, leading to its degradation by the proteasome. Ubiquitination is mediated via its interaction with FZR1/CDH1. Following DNA damage, it is deubiquitinated by USP28 in G2 phase, preventing its degradation By similarity.
Sequence similarities	Belongs to the claspin family.

Ontologies

Keywords
Biological process	Cell cycle DNA damage DNA repair
Cellular component	Chromosome Nucleus
Domain	Coiled coil Repeat
Ligand	DNA-binding
PTM	Acetylation Phosphoprotein Ubl conjugation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	DNA repair Inferred from electronic annotation. Source: UniProtKB-KW G2 DNA damage checkpoint Inferred from sequence or structural similarity. Source: UniProtKB activation of protein kinase activity Inferred from electronic annotation. Source: Compara mitotic DNA replication checkpoint Inferred from electronic annotation. Source: Compara peptidyl-serine phosphorylation Inferred from electronic annotation. Source: Compara
Cellular_component	chromosome Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 1315

1315

Claspin

PRO_0000089876

Regions

Repeat

887 – 896

CKB motif 1

Repeat

917 – 926

CKB motif 2

Repeat

954 – 963

CKB motif 3

Coiled coil

159 – 187

Potential

Coiled coil

599 – 626

Potential

Coiled coil

1001 – 1036

Potential

Amino acid modifications

Modified residue

Phosphoserine By similarity

Modified residue

Phosphoserine By similarity

Modified residue

220

Phosphoserine By similarity

Modified residue

698

Phosphoserine By similarity

Modified residue

701

Phosphoserine By similarity

Modified residue

785

Phosphoserine By similarity

Modified residue

787

Phosphoserine By similarity

Modified residue

810

Phosphoserine By similarity

Modified residue

816

Phosphoserine By similarity

Modified residue

823

Phosphoserine By similarity

Modified residue

868

N6-acetyllysine By similarity

Modified residue

893

Phosphothreonine; by CHEK1 By similarity

Modified residue

990

Phosphoserine By similarity

Modified residue

996

Phosphoserine By similarity

Modified residue

998

Phosphoserine By similarity

Modified residue

1133

Phosphoserine By similarity

Modified residue

1265

Phosphoserine By similarity

Experimental info

Sequence conflict

727

Y → H in AAH50848. Ref.2

Sequence conflict

1013

E → EE in AAP97538. Ref.3

Sequences

Sequence

Length

Mass (Da)

Tools

Q80YR7 [UniParc].

Last modified July 27, 2011. Version 2.
Checksum: 762AA39E19D8EAA3
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FASTA

1,315

146,715

        10         20         30         40         50         60 
MTGEVGSEVN LEVNDLKLLS QEAADSPVDS GQGSFETLEP LSERDSDEEI FVSKKPKSRK 

        70         80         90        100        110        120 
VLQDSDSEAE DRDDAPEKPT YDDSAEDTQE NLHSGKSQSR SFPKALADSD ESDMEETPSQ 

       130        140        150        160        170        180 
ESPETQEAPS LEPGHQTGHS VDFTTGRKLS KTLLREGAEG KAKSKRRLEK EERTMEKIRR 

       190        200        210        220        230        240 
LKKKETRCEE SDADRPLNDS GCLLEDSDLF ETGLEEENDS ALEDEESLES IRAAVKNKVK 

       250        260        270        280        290        300 
NRKKKEPTLE SEAFSLEDGN ELSKGSARKE RKAARLSKEA LKKLHSETQR LVRESALNLP 

       310        320        330        340        350        360 
YHMPESKTIH DFFKRKPRPT CQGSAMALLK SCKYQSGHYK ETVNPADAAG MGAEDSSRGS 

       370        380        390        400        410        420 
EQRTGAGIAA ETNVLSEVSE EAGITAGSDE ACGKDPVRRG ELEIEETEKH SDDRPYSPGD 

       430        440        450        460        470        480 
RSMSQQESSI PRIEDNEGHQ AGDLTESDPP ALEGEELKTV EKTDAKEGMP EQKTQSAAAA 

       490        500        510        520        530        540 
AVAVVTAAAA PPEKVRRFTV DRLRQLGVDV SSQPRLGADE DSFVILDEPK TNRELEALKQ 

       550        560        570        580        590        600 
RFWRHANPAA SPRACQTVNV NIIVKDLGTN GKEELKAEVV PVTLAAEKLE GASHAKPGEK 

       610        620        630        640        650        660 
LQMLKAKLQE AMKLRRLEER QKRQALFKLD NEDGFEEEEE EEEMTDESEE DGEEETTEYL 

       670        680        690        700        710        720 
LGSEDTETKD EKETDKENTD TSSDIGKSVA LCVPKPLSSD STLLLFKDSS SKMGYFPTEE 

       730        740        750        760        770        780 
KSETDEYLAK QSDKLDEDDS SSLLTKESSH NSSFELIGST IPSYQPCNRQ IGRGASFLPT 

       790        800        810        820        830        840 
AGFRSPSPGL FRGSLISSAS KSSGKLSEPS LPVEDSQDLY TASPEPKTLF LGAGDFQFCL 

       850        860        870        880        890        900 
EDDTQSQLLD ADGFLNIRNH RHRYQAVKPQ LPLASMDENA MDANMDELLD LCTGQFTSQP 

       910        920        930        940        950        960 
EEKCQPRKND KKENMEELLN LCSGKFPTQD ASPVAPLGLR SQEKESSTED PMEEALALCS 

       970        980        990       1000       1010       1020 
GSFPTDREEE GEEEEFGDFQ LVSKENGFAS DEDEHSDSND EELALDLEDD EEELLKQSEK 

      1030       1040       1050       1060       1070       1080 
MKRQMRLKKY LEDEAEVSGS DVGSEDEYDG EEIDEYEEDV IDEVLPSDEE LESQIKKIHM 

      1090       1100       1110       1120       1130       1140 
KTMLDDDKRR LRLYQERYLA DGDLHSDGPG RTRKFRWKHI DDTSQMDLFH RDSDDDQVEE 

      1150       1160       1170       1180       1190       1200 
QLDETEAKWR KERIEREQWL REQAQQGKIA ADEEDIGDDS QFMMLAKKVT AKALQKNASH 

      1210       1220       1230       1240       1250       1260 
TVVVQESKSV LRNPFETIRP GGAHQLKTGS LLNQPKAVLQ KLAALSDLNP SAPRNSRNFV 

      1270       1280       1290       1300       1310 
FHTLSPTKAE AAKDSSKPQV RRRGLSSMMS PSPKRLKTNG SSPGPKRSIF RYLES

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6J.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Limb.
[3]	"Checkpoint control in gametogenesis." Hasthorpe S. Submitted (JUN-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 906-1315. Strain: ICR. Tissue: Testis.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL606935 Genomic DNA. Translation: CAM17430.1. BC050848 mRNA. Translation: AAH50848.1. AY324187 mRNA. Translation: AAP97538.1.
IPI	IPI00330880.
RefSeq	NP_780763.3. NM_175554.4.
UniGene	Mm.243962.
3D structure databases
ModBase	Search...
PTM databases
PhosphoSite	Q80YR7.
Proteomic databases
PaxDb	Q80YR7.
PRIDE	Q80YR7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000048391; ENSMUSP00000045344; ENSMUSG00000042489.
GeneID	269582.
KEGG	mmu:269582.
Organism-specific databases
CTD	63967.
MGI	MGI:2445153. Clspn.
Phylogenomic databases
eggNOG	NOG314362.
GeneTree	ENSGT00390000012738.
HOGENOM	HOG000049140.
HOVERGEN	HBG080104.
InParanoid	B1ARX6.
OMA	KKLHSET.
OrthoDB	EOG4PG60X.
Gene expression databases
ArrayExpress	Q80YR7.
Bgee	Q80YR7.
CleanEx	MM_CLSPN.
Genevestigator	Q80YR7.
GermOnline	ENSMUSG00000042489. Mus musculus.
Family and domain databases
InterPro	IPR024147. Claspin. IPR024146. Claspin/Claspin-like. [Graphical view]
PANTHER	PTHR14396. PTHR14396. 1 hit. PTHR14396:SF4. PTHR14396:SF4. 1 hit.
ProtoNet	Search...
Other
NextBio	392895.
SOURCE	Search...

Entry information

Entry name

CLSPN_MOUSE

Accession

Primary (citable) accession number: Q80YR7
Secondary accession number(s): B1ARX6, Q69GM2

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 30, 2005
Last sequence update:	July 27, 2011
Last modified:	May 1, 2013
This is version 64 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents